Neorhizobium sp. NCHU2750

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Neorhizobium; unclassified Neorhizobium

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386DVE1|A0A386DVE1_9RHIZ Amino acid ABC transporter permease OS=Neorhizobium sp. NCHU2750 OX=1825976 GN=NCHU2750_12160 PE=3 SV=1
MM1 pKa = 7.43LLKK4 pKa = 10.77SKK6 pKa = 9.26VLCLLASSALVGIVSLASTANASVGSDD33 pKa = 2.87ILGLSSDD40 pKa = 3.67GSVMLNYY47 pKa = 10.48SYY49 pKa = 11.41DD50 pKa = 3.68ADD52 pKa = 4.39TEE54 pKa = 4.43TGTYY58 pKa = 10.98LLTTTSTGEE67 pKa = 4.19TQQITPPAGAMSVYY81 pKa = 10.13PSALSADD88 pKa = 3.78GKK90 pKa = 9.01TVTGYY95 pKa = 10.99YY96 pKa = 8.71VTDD99 pKa = 4.57AYY101 pKa = 11.21QKK103 pKa = 10.88VAFVWTAAGGFVDD116 pKa = 5.88LGVLDD121 pKa = 4.93EE122 pKa = 4.75DD123 pKa = 4.15TTGAFAQSVSGDD135 pKa = 3.25GSAVAGYY142 pKa = 10.35VSIDD146 pKa = 3.03SYY148 pKa = 10.33MAEE151 pKa = 3.8GFYY154 pKa = 10.27WSSSTGMVGIGTLGGRR170 pKa = 11.84DD171 pKa = 3.31SYY173 pKa = 12.25ANAISTDD180 pKa = 2.74GSTVVGRR187 pKa = 11.84ADD189 pKa = 3.23TADD192 pKa = 3.53EE193 pKa = 4.26VFHH196 pKa = 6.74AFVWSVDD203 pKa = 3.44TNTMQNIDD211 pKa = 3.65TLYY214 pKa = 8.72TSSSATLVSDD224 pKa = 4.94DD225 pKa = 3.52GTAVAGTGSTGTTSGVFRR243 pKa = 11.84WTADD247 pKa = 2.66TGMVDD252 pKa = 2.79IGTLGGIYY260 pKa = 9.93TSVAAMSGDD269 pKa = 3.47GNVLVGQSTTVGEE282 pKa = 4.47SDD284 pKa = 3.42YY285 pKa = 11.24HH286 pKa = 7.22AYY288 pKa = 10.22RR289 pKa = 11.84YY290 pKa = 8.91VASTSTMTDD299 pKa = 2.84LGTLGGTYY307 pKa = 9.98SYY309 pKa = 11.63ASDD312 pKa = 3.64LTADD316 pKa = 3.71GSIVVGRR323 pKa = 11.84ASDD326 pKa = 3.52SASAYY331 pKa = 10.43HH332 pKa = 6.04GFVWSEE338 pKa = 3.74ATGMQTVEE346 pKa = 4.31DD347 pKa = 4.18WLTAHH352 pKa = 7.4GATLEE357 pKa = 4.05GDD359 pKa = 3.61MTTSADD365 pKa = 3.99KK366 pKa = 10.72ISADD370 pKa = 3.07GSVIVGSTTSGSTYY384 pKa = 9.18IARR387 pKa = 11.84VVTTGSDD394 pKa = 3.36DD395 pKa = 3.61STGGDD400 pKa = 3.09SGGGDD405 pKa = 3.8SEE407 pKa = 5.29CNADD411 pKa = 3.47VCDD414 pKa = 4.27GGGEE418 pKa = 4.09SGIIDD423 pKa = 3.6TAKK426 pKa = 10.52YY427 pKa = 9.65FPTVASANNMIVQSGVNSADD447 pKa = 3.14TIMFGAQGAPMRR459 pKa = 11.84NLLKK463 pKa = 10.05TGQKK467 pKa = 10.0SVWGTVDD474 pKa = 3.41GGYY477 pKa = 10.55DD478 pKa = 4.11DD479 pKa = 5.82SDD481 pKa = 3.91HH482 pKa = 7.27ADD484 pKa = 3.36GGLALGDD491 pKa = 3.66FGFGYY496 pKa = 10.79GIAEE500 pKa = 4.16GVTARR505 pKa = 11.84FSAGMTYY512 pKa = 10.19TDD514 pKa = 3.33QDD516 pKa = 3.6LDD518 pKa = 3.46AGGDD522 pKa = 3.34VRR524 pKa = 11.84QRR526 pKa = 11.84GFYY529 pKa = 10.58LSPEE533 pKa = 3.99VSADD537 pKa = 3.29LGRR540 pKa = 11.84NVYY543 pKa = 7.73MTVGGYY549 pKa = 8.24WGRR552 pKa = 11.84SSIDD556 pKa = 2.95SRR558 pKa = 11.84RR559 pKa = 11.84GYY561 pKa = 11.27ASGAITDD568 pKa = 3.68YY569 pKa = 11.38SYY571 pKa = 12.1GDD573 pKa = 3.67TNAEE577 pKa = 3.61TWGAKK582 pKa = 9.1IRR584 pKa = 11.84FDD586 pKa = 3.61WLNAATIADD595 pKa = 4.1TAITPYY601 pKa = 10.89AGLSYY606 pKa = 11.16AHH608 pKa = 6.19TRR610 pKa = 11.84VDD612 pKa = 3.56GFSEE616 pKa = 4.14TGGAFPVEE624 pKa = 4.21FDD626 pKa = 3.74GSSDD630 pKa = 3.08HH631 pKa = 6.65ATIARR636 pKa = 11.84LGSDD640 pKa = 3.85FVRR643 pKa = 11.84PLNDD647 pKa = 3.37TVRR650 pKa = 11.84LLAKK654 pKa = 10.58AEE656 pKa = 3.94LDD658 pKa = 3.76YY659 pKa = 11.43QFEE662 pKa = 4.15NHH664 pKa = 6.24AAATTGTLTGISDD677 pKa = 4.48FDD679 pKa = 5.42LEE681 pKa = 4.54GQDD684 pKa = 4.99LQQFWVRR691 pKa = 11.84GGLGAEE697 pKa = 3.87FDD699 pKa = 3.79VGKK702 pKa = 10.65GVASFMVNATTKK714 pKa = 10.62GQDD717 pKa = 3.08PTVWLRR723 pKa = 11.84SNYY726 pKa = 7.13TVKK729 pKa = 10.76FF730 pKa = 3.7

Molecular weight: 75.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386E6G1|A0A386E6G1_9RHIZ Amino acid ABC transporter substrate-binding protein OS=Neorhizobium sp. NCHU2750 OX=1825976 GN=NCHU2750_45240 PE=3 SV=1
MM1 pKa = 7.66PGKK4 pKa = 10.03SPSSGGVAVKK14 pKa = 10.01GVRR17 pKa = 11.84RR18 pKa = 11.84SALRR22 pKa = 11.84MASTLAAGACGALLAKK38 pKa = 10.38LVGVPLPFLLGPLVFGAAATLAGAPVLTVPYY69 pKa = 9.56GRR71 pKa = 11.84QIGQVVVGVSIGLRR85 pKa = 11.84FIPSVAVATLTLIPVMVAVTVLVIVATSIAALLLMRR121 pKa = 11.84LGGVDD126 pKa = 3.31RR127 pKa = 11.84EE128 pKa = 4.4TAFFATAAAGLAEE141 pKa = 4.33MAVVAQNKK149 pKa = 8.34GANPDD154 pKa = 3.08IVAVVHH160 pKa = 6.51LIRR163 pKa = 11.84VTSIVTSVPILLAIFGHH180 pKa = 6.96PGDD183 pKa = 4.3VPPVAVPLAAEE194 pKa = 4.62LLPLAGLLVVSGIAAYY210 pKa = 10.06LVAPFGMPNTWLLIPTLIGALVALSGFGPFAVPKK244 pKa = 10.45LLLNLAQIVIGTWIGCRR261 pKa = 11.84FRR263 pKa = 11.84RR264 pKa = 11.84EE265 pKa = 3.43IVARR269 pKa = 11.84LPRR272 pKa = 11.84VSLAAVATTAFLLASAALIASLVCWMTGLPFSTAFLSISPAGVTEE317 pKa = 4.21MVLTATAMHH326 pKa = 7.1LDD328 pKa = 3.5AATVTGFQIMRR339 pKa = 11.84IAVVMTTIPLVFRR352 pKa = 11.84LFAYY356 pKa = 10.62LSAPDD361 pKa = 4.45ADD363 pKa = 5.38RR364 pKa = 11.84IDD366 pKa = 3.6APRR369 pKa = 11.84PP370 pKa = 3.45

Molecular weight: 37.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5901

0

5901

1837426

29

2828

311.4

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.821 ± 0.042

0.782 ± 0.01

5.788 ± 0.032

5.61 ± 0.024

3.872 ± 0.018

8.319 ± 0.031

1.974 ± 0.015

5.802 ± 0.024

3.83 ± 0.026

9.851 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.016

2.864 ± 0.019

4.854 ± 0.022

3.183 ± 0.025

6.482 ± 0.037

6.016 ± 0.026

5.434 ± 0.028

7.25 ± 0.028

1.253 ± 0.013

2.322 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski