Streptomyces phage Nerdos

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Likavirus; unclassified Likavirus

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CVH1|A0A411CVH1_9CAUD Uncharacterized protein OS=Streptomyces phage Nerdos OX=2510519 GN=65 PE=4 SV=1
MM1 pKa = 7.45SKK3 pKa = 10.54NVVKK7 pKa = 10.49IGMVSVDD14 pKa = 3.28SGTVFVGDD22 pKa = 3.29PCYY25 pKa = 10.19TVTGDD30 pKa = 3.5ASNHH34 pKa = 4.43VEE36 pKa = 4.77TWSEE40 pKa = 3.68WCDD43 pKa = 3.27RR44 pKa = 11.84SPFGAFPFDD53 pKa = 3.33VTEE56 pKa = 4.01PVGPGLGLSIPTYY69 pKa = 9.45WGDD72 pKa = 2.94GGYY75 pKa = 9.48PVYY78 pKa = 11.1AEE80 pKa = 4.21VEE82 pKa = 3.96NGRR85 pKa = 11.84VKK87 pKa = 10.61RR88 pKa = 11.84VTIDD92 pKa = 3.59FDD94 pKa = 3.81PSYY97 pKa = 11.26DD98 pKa = 3.75EE99 pKa = 5.07EE100 pKa = 5.0DD101 pKa = 4.01FEE103 pKa = 5.08

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CVD4|A0A411CVD4_9CAUD Uncharacterized protein OS=Streptomyces phage Nerdos OX=2510519 GN=25 PE=4 SV=1
MM1 pKa = 7.22SVQRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GQVARR12 pKa = 11.84IWKK15 pKa = 4.84TTKK18 pKa = 9.45VTDD21 pKa = 3.28NRR23 pKa = 11.84GNEE26 pKa = 3.7VHH28 pKa = 6.2VANNDD33 pKa = 3.34GPHH36 pKa = 5.82EE37 pKa = 4.1VRR39 pKa = 11.84AAFIPQRR46 pKa = 11.84SAKK49 pKa = 10.63AEE51 pKa = 4.13VPGQMQINITRR62 pKa = 11.84MIVAADD68 pKa = 3.77LEE70 pKa = 4.88GVEE73 pKa = 4.06LWSRR77 pKa = 11.84VEE79 pKa = 3.94WQGKK83 pKa = 6.2QWDD86 pKa = 4.21IVTPPAYY93 pKa = 10.07HH94 pKa = 6.56HH95 pKa = 5.93GTRR98 pKa = 11.84HH99 pKa = 4.58TRR101 pKa = 11.84HH102 pKa = 5.28WSIDD106 pKa = 2.96IRR108 pKa = 11.84EE109 pKa = 4.09RR110 pKa = 11.84PP111 pKa = 3.36

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14913

41

1159

207.1

22.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.628 ± 0.369

0.65 ± 0.106

6.451 ± 0.286

7.276 ± 0.391

3.185 ± 0.203

8.503 ± 0.358

1.965 ± 0.205

4.56 ± 0.297

4.359 ± 0.254

8.449 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.161

3.031 ± 0.142

4.915 ± 0.251

3.105 ± 0.128

6.364 ± 0.329

6.035 ± 0.28

6.25 ± 0.259

7.289 ± 0.244

1.837 ± 0.134

2.997 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski