Colletotrichum camelliae filamentous virus 1
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286M3N6|A0A286M3N6_9VIRU Uncharacterized protein OS=Colletotrichum camelliae filamentous virus 1 OX=2029458 PE=4 SV=1
MM1 pKa = 7.33 VNEE4 pKa = 4.54 VISNAEE10 pKa = 4.01 VGPGITMVRR19 pKa = 11.84 LGRR22 pKa = 11.84 DD23 pKa = 3.19 DD24 pKa = 3.81 MEE26 pKa = 4.52 GVISDD31 pKa = 3.59 FTRR34 pKa = 11.84 THH36 pKa = 6.0 ILDD39 pKa = 3.59 PRR41 pKa = 11.84 FSLSFCIAIYY51 pKa = 10.6 NVIQYY56 pKa = 10.21 PEE58 pKa = 4.61 GYY60 pKa = 9.71 EE61 pKa = 4.01 VADD64 pKa = 4.03 KK65 pKa = 11.17 GNSFGRR71 pKa = 11.84 IVIIADD77 pKa = 3.59 YY78 pKa = 9.95 EE79 pKa = 4.16 WRR81 pKa = 11.84 GLEE84 pKa = 3.76 KK85 pKa = 10.76 DD86 pKa = 3.56 LAIGVWFPDD95 pKa = 3.3 LKK97 pKa = 11.1 AFCFVDD103 pKa = 3.54 LHH105 pKa = 6.58 PVLSDD110 pKa = 3.66 PPNLRR115 pKa = 11.84 PNCWVDD121 pKa = 3.22 VLTGVAGQGVIVTALLGASARR142 pKa = 11.84 LLQDD146 pKa = 3.75 RR147 pKa = 11.84 YY148 pKa = 10.15 LHH150 pKa = 6.36 KK151 pKa = 11.23 SPDD154 pKa = 3.55 KK155 pKa = 10.6 FSCRR159 pKa = 11.84 YY160 pKa = 9.6 EE161 pKa = 4.69 DD162 pKa = 4.06 DD163 pKa = 3.75 SKK165 pKa = 11.86 LPGVQFAGRR174 pKa = 11.84 NVEE177 pKa = 4.31 YY178 pKa = 11.08 NRR180 pKa = 11.84 MAHH183 pKa = 6.32 GLAVPTSADD192 pKa = 3.09 EE193 pKa = 4.79 GGSVLYY199 pKa = 9.89 PDD201 pKa = 4.27 YY202 pKa = 11.14 DD203 pKa = 3.69 SSEE206 pKa = 4.09 EE207 pKa = 4.19 SEE209 pKa = 4.18
Molecular weight: 23.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.499
IPC2_protein 4.558
IPC_protein 4.507
Toseland 4.329
ProMoST 4.584
Dawson 4.469
Bjellqvist 4.647
Wikipedia 4.368
Rodwell 4.342
Grimsley 4.24
Solomon 4.469
Lehninger 4.418
Nozaki 4.584
DTASelect 4.787
Thurlkill 4.355
EMBOSS 4.38
Sillero 4.622
Patrickios 3.567
IPC_peptide 4.469
IPC2_peptide 4.609
IPC2.peptide.svr19 4.574
Protein with the highest isoelectric point:
>tr|A0A286M3N4|A0A286M3N4_9VIRU Uncharacterized protein OS=Colletotrichum camelliae filamentous virus 1 OX=2029458 PE=4 SV=1
MM1 pKa = 7.88 PFFSHH6 pKa = 6.94 RR7 pKa = 11.84 RR8 pKa = 11.84 FLFFFSAVVIGPDD21 pKa = 3.07 RR22 pKa = 11.84 SNPRR26 pKa = 11.84 APRR29 pKa = 11.84 RR30 pKa = 11.84 GWWNVPRR37 pKa = 11.84 DD38 pKa = 3.7 AVV40 pKa = 3.35
Molecular weight: 4.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.182
IPC2_protein 10.35
IPC_protein 11.959
Toseland 12.12
ProMoST 12.632
Dawson 12.12
Bjellqvist 12.12
Wikipedia 12.603
Rodwell 11.623
Grimsley 12.164
Solomon 12.632
Lehninger 12.53
Nozaki 12.12
DTASelect 12.12
Thurlkill 12.12
EMBOSS 12.632
Sillero 12.12
Patrickios 11.418
IPC_peptide 12.632
IPC2_peptide 11.623
IPC2.peptide.svr19 9.429
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
3289
40
771
365.4
39.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.125 ± 0.764
1.459 ± 0.26
6.841 ± 0.508
4.439 ± 0.37
3.588 ± 0.388
7.936 ± 0.572
1.976 ± 0.244
2.615 ± 0.351
2.615 ± 0.448
8.392 ± 0.703
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.524 ± 0.277
2.463 ± 0.194
6.719 ± 0.518
1.763 ± 0.252
8.696 ± 0.642
8.878 ± 0.91
5.412 ± 0.386
10.125 ± 0.632
0.912 ± 0.159
2.524 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here