Streptococcus virus 2972
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q56S78|Q56S78_9CAUD Uncharacterized protein OS=Streptococcus virus 2972 OX=306323 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.58 ANGNDD11 pKa = 3.45 IPDD14 pKa = 4.08 GKK16 pKa = 10.99 SEE18 pKa = 4.08 DD19 pKa = 4.06 FKK21 pKa = 11.35 PGMFFSFAGDD31 pKa = 3.45 EE32 pKa = 4.32 VNITDD37 pKa = 3.77 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.841
IPC2_protein 3.999
IPC_protein 3.897
Toseland 3.694
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.872
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.872
Sillero 4.024
Patrickios 0.477
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|Q56S90|Q56S90_9CAUD Uncharacterized protein OS=Streptococcus virus 2972 OX=306323 PE=4 SV=1
MM1 pKa = 7.83 ADD3 pKa = 3.75 FGVSLLEE10 pKa = 4.01 ARR12 pKa = 11.84 RR13 pKa = 11.84 MTLKK17 pKa = 10.49 EE18 pKa = 3.53 MKK20 pKa = 9.92 LYY22 pKa = 10.32 QKK24 pKa = 10.55 AYY26 pKa = 9.84 KK27 pKa = 10.03 KK28 pKa = 10.52 RR29 pKa = 11.84 FLNKK33 pKa = 9.43 EE34 pKa = 3.69 RR35 pKa = 11.84 EE36 pKa = 4.36 IYY38 pKa = 9.68 QLAYY42 pKa = 10.87 LNRR45 pKa = 11.84 LANATTKK52 pKa = 10.62 DD53 pKa = 3.03 GKK55 pKa = 10.64 KK56 pKa = 10.46 YY57 pKa = 10.49 YY58 pKa = 10.19 FEE60 pKa = 5.48 KK61 pKa = 10.83 FDD63 pKa = 3.95 DD64 pKa = 4.49 FYY66 pKa = 11.56 NAKK69 pKa = 9.54 EE70 pKa = 3.83 RR71 pKa = 11.84 ARR73 pKa = 11.84 EE74 pKa = 3.84 VLGEE78 pKa = 4.35 KK79 pKa = 8.61 ITNSKK84 pKa = 10.24 LLEE87 pKa = 4.07 RR88 pKa = 11.84 AKK90 pKa = 11.02 KK91 pKa = 8.79 NLNYY95 pKa = 10.35 KK96 pKa = 9.9 LEE98 pKa = 4.59 RR99 pKa = 11.84 GLLDD103 pKa = 3.37 GRR105 pKa = 4.46
Molecular weight: 12.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.08
IPC2_protein 9.341
IPC_protein 9.297
Toseland 10.014
ProMoST 9.736
Dawson 10.204
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.701
Grimsley 10.277
Solomon 10.233
Lehninger 10.189
Nozaki 9.97
DTASelect 9.853
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.101
Patrickios 10.292
IPC_peptide 10.233
IPC2_peptide 8.302
IPC2.peptide.svr19 8.348
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
10805
40
1605
245.6
27.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.959 ± 1.269
0.509 ± 0.112
6.145 ± 0.57
6.312 ± 0.758
3.97 ± 0.172
7.265 ± 0.568
1.175 ± 0.194
6.839 ± 0.435
7.552 ± 0.674
7.441 ± 0.457
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.49 ± 0.318
5.599 ± 0.403
2.952 ± 0.302
3.739 ± 0.287
4.174 ± 0.448
7.145 ± 0.713
6.876 ± 0.499
6.645 ± 0.293
1.305 ± 0.201
3.906 ± 0.491
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here