Synechococcus sp. RS9916

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; unclassified Synechococcus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q05SH5|Q05SH5_9SYNE Uncharacterized protein OS=Synechococcus sp. RS9916 OX=221359 GN=RS9916_25629 PE=4 SV=1
MM1 pKa = 7.79IPIKK5 pKa = 10.56GWGVDD10 pKa = 3.49HH11 pKa = 7.69GDD13 pKa = 3.84GEE15 pKa = 5.17CVGGRR20 pKa = 11.84PPQHH24 pKa = 6.64INPNNTMPFTFLNFNQKK41 pKa = 10.11LFFGDD46 pKa = 3.82SQDD49 pKa = 4.4DD50 pKa = 3.73AVLSLGSSKK59 pKa = 10.82VINLGGGNNTIYY71 pKa = 11.04SFGTNNTATSGSGDD85 pKa = 3.38DD86 pKa = 3.49RR87 pKa = 11.84FYY89 pKa = 10.83MIGGDD94 pKa = 3.47QTIVSTGGDD103 pKa = 3.22NTAITSSGDD112 pKa = 3.23DD113 pKa = 3.47RR114 pKa = 11.84IIFSNQIGGNDD125 pKa = 3.61YY126 pKa = 10.51VRR128 pKa = 11.84SGSGEE133 pKa = 3.94DD134 pKa = 3.42VIYY137 pKa = 10.5TSGGDD142 pKa = 3.81DD143 pKa = 3.69EE144 pKa = 4.83VHH146 pKa = 6.74SGADD150 pKa = 3.22NDD152 pKa = 3.84QVYY155 pKa = 10.45LGSGNDD161 pKa = 3.4TAYY164 pKa = 10.76LGSGDD169 pKa = 3.86DD170 pKa = 3.31VAYY173 pKa = 10.51AGTGTDD179 pKa = 5.09LIDD182 pKa = 4.93GEE184 pKa = 5.14DD185 pKa = 3.93GNDD188 pKa = 3.9TISGLHH194 pKa = 5.75SKK196 pKa = 10.55GNKK199 pKa = 7.7TFFGRR204 pKa = 11.84AGDD207 pKa = 3.8DD208 pKa = 3.47TLTGGVHH215 pKa = 6.83NDD217 pKa = 3.59YY218 pKa = 11.31LDD220 pKa = 4.01GGADD224 pKa = 3.53ADD226 pKa = 3.87VLTGNEE232 pKa = 4.15GFNIYY237 pKa = 9.34WGGDD241 pKa = 3.34DD242 pKa = 4.94ADD244 pKa = 3.61VFRR247 pKa = 11.84IGRR250 pKa = 11.84GVDD253 pKa = 4.02LIEE256 pKa = 5.19DD257 pKa = 4.06FNLAEE262 pKa = 4.82GDD264 pKa = 3.82TIEE267 pKa = 4.28MVEE270 pKa = 4.69PIASICASVSAHH282 pKa = 4.32VVNYY286 pKa = 10.64SGTLVNGQSISGSIVCEE303 pKa = 4.19TEE305 pKa = 3.39NQARR309 pKa = 11.84DD310 pKa = 4.18FYY312 pKa = 10.59FNWMPQASS320 pKa = 3.6

Molecular weight: 33.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q05WM3|Q05WM3_9SYNE Uncharacterized protein OS=Synechococcus sp. RS9916 OX=221359 GN=RS9916_33607 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.21TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.74RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.99

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2955

0

2955

801681

20

7750

271.3

29.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.756 ± 0.063

1.185 ± 0.024

5.363 ± 0.051

5.458 ± 0.062

2.957 ± 0.04

8.83 ± 0.19

2.057 ± 0.039

4.177 ± 0.048

2.712 ± 0.048

12.063 ± 0.098

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.036 ± 0.03

2.913 ± 0.076

5.371 ± 0.072

4.868 ± 0.052

6.765 ± 0.089

6.667 ± 0.097

5.28 ± 0.123

7.013 ± 0.047

1.721 ± 0.032

1.808 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski