Geobacter sp. OR-1
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A8WXA3|A0A0A8WXA3_9DELT (R)-citramalate synthase OS=Geobacter sp. OR-1 OX=1266765 GN=OR1_02959 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.97 IEE4 pKa = 4.04 YY5 pKa = 8.86 PAIFDD10 pKa = 4.07 PADD13 pKa = 3.46 EE14 pKa = 4.69 GGYY17 pKa = 9.22 TITFHH22 pKa = 7.47 DD23 pKa = 4.53 FPEE26 pKa = 5.24 AISEE30 pKa = 4.12 GDD32 pKa = 3.53 TLEE35 pKa = 4.21 EE36 pKa = 3.34 AHH38 pKa = 6.56 YY39 pKa = 10.78 NAIEE43 pKa = 4.02 VLDD46 pKa = 4.05 LTLQSRR52 pKa = 11.84 MEE54 pKa = 4.64 DD55 pKa = 3.32 NEE57 pKa = 4.74 IIPLPHH63 pKa = 6.59 SEE65 pKa = 4.1 SGANVHH71 pKa = 6.72 MIAPDD76 pKa = 3.66 VNIQAALLSIGG87 pKa = 3.67
Molecular weight: 9.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.732
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.139
Thurlkill 3.77
EMBOSS 3.795
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A0A8WLY5|A0A0A8WLY5_9DELT 60 kDa chaperonin OS=Geobacter sp. OR-1 OX=1266765 GN=groL PE=3 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.15 EE3 pKa = 3.99 LRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 8.83 MLARR11 pKa = 11.84 LSPQIAWLIYY21 pKa = 10.27 GLLSLFRR28 pKa = 11.84 MTMRR32 pKa = 11.84 IRR34 pKa = 11.84 MIGGEE39 pKa = 4.07 IMPGLVARR47 pKa = 11.84 GEE49 pKa = 4.35 GFIGTFWHH57 pKa = 6.74 ARR59 pKa = 11.84 LLMIPFIYY67 pKa = 10.0 PGTRR71 pKa = 11.84 MNVLIGTHH79 pKa = 6.92 RR80 pKa = 11.84 DD81 pKa = 3.25 GQLIADD87 pKa = 3.76 VMKK90 pKa = 10.82 CFGFGLVRR98 pKa = 11.84 GSSSKK103 pKa = 11.06 GGVGALRR110 pKa = 11.84 EE111 pKa = 4.15 MGRR114 pKa = 11.84 LLAKK118 pKa = 10.6 GNDD121 pKa = 3.48 VAITPDD127 pKa = 3.74 GPRR130 pKa = 11.84 GPAEE134 pKa = 3.91 VAKK137 pKa = 10.44 MGVAQVARR145 pKa = 11.84 VTGRR149 pKa = 11.84 PVVPIAFSSSRR160 pKa = 11.84 CWRR163 pKa = 11.84 GTSWDD168 pKa = 3.33 RR169 pKa = 11.84 MVIPKK174 pKa = 9.67 PFSQGVFVIGDD185 pKa = 3.45 PVYY188 pKa = 9.97 YY189 pKa = 10.47 SEE191 pKa = 5.11 GEE193 pKa = 3.89 EE194 pKa = 4.3 LEE196 pKa = 4.29 TFRR199 pKa = 11.84 LRR201 pKa = 11.84 IEE203 pKa = 3.85 TALRR207 pKa = 11.84 EE208 pKa = 4.28 TTATADD214 pKa = 3.58 RR215 pKa = 11.84 AADD218 pKa = 3.52 SRR220 pKa = 11.84 QQ221 pKa = 3.04
Molecular weight: 24.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.56
IPC_protein 10.496
Toseland 10.657
ProMoST 10.423
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.847
Grimsley 10.804
Solomon 10.906
Lehninger 10.862
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.57
IPC_peptide 10.906
IPC2_peptide 9.56
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4153
0
4153
1364415
30
4526
328.5
36.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.301 ± 0.046
1.347 ± 0.027
5.229 ± 0.025
6.2 ± 0.05
4.045 ± 0.029
7.935 ± 0.049
1.99 ± 0.027
6.383 ± 0.039
4.992 ± 0.043
10.024 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.457 ± 0.021
3.723 ± 0.035
4.497 ± 0.029
3.163 ± 0.024
5.828 ± 0.044
6.369 ± 0.047
5.493 ± 0.06
7.046 ± 0.033
1.078 ± 0.015
2.899 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here