Bat circovirus POA/2012/VI
Average proteome isoelectric point is 9.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0YUE0|A0A0A0YUE0_9CIRC Capsid OS=Bat circovirus POA/2012/VI OX=1572239 GN=Cap PE=4 SV=1
MM1 pKa = 7.62 AFRR4 pKa = 11.84 RR5 pKa = 11.84 FRR7 pKa = 11.84 RR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 MPLRR15 pKa = 11.84 RR16 pKa = 11.84 NRR18 pKa = 11.84 FRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 IFRR25 pKa = 11.84 HH26 pKa = 4.88 RR27 pKa = 11.84 LRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 VVRR34 pKa = 11.84 RR35 pKa = 11.84 TPTLYY40 pKa = 10.89 VKK42 pKa = 9.38 LTRR45 pKa = 11.84 TIQIAIPPSVEE56 pKa = 3.96 FNQPLHH62 pKa = 6.64 VSLNDD67 pKa = 3.48 FAEE70 pKa = 5.04 HH71 pKa = 6.88 INLAPNFEE79 pKa = 4.3 RR80 pKa = 11.84 VKK82 pKa = 10.84 ALRR85 pKa = 11.84 QTVRR89 pKa = 11.84 VYY91 pKa = 10.06 PQQNVSNTSTSRR103 pKa = 11.84 VGNYY107 pKa = 10.15 CMLPYY112 pKa = 10.0 HH113 pKa = 6.51 KK114 pKa = 9.68 PVPTSLINFPTALSVDD130 pKa = 3.25 KK131 pKa = 11.18 AKK133 pKa = 10.75 VFRR136 pKa = 11.84 CTQKK140 pKa = 10.55 GRR142 pKa = 11.84 MSFVPATRR150 pKa = 11.84 LDD152 pKa = 3.59 ADD154 pKa = 3.94 GVGGNQTIRR163 pKa = 11.84 TDD165 pKa = 3.05 WRR167 pKa = 11.84 PEE169 pKa = 3.81 LEE171 pKa = 4.32 IGSTATNVIMYY182 pKa = 8.82 TGMIVFEE189 pKa = 4.92 NINAGLTGTTAYY201 pKa = 8.76 YY202 pKa = 9.95 TVVQDD207 pKa = 4.33 LYY209 pKa = 11.69 VRR211 pKa = 11.84 YY212 pKa = 9.71 KK213 pKa = 9.84 NQRR216 pKa = 11.84 SFII219 pKa = 4.23
Molecular weight: 25.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.174
IPC2_protein 10.262
IPC_protein 11.447
Toseland 11.52
ProMoST 11.974
Dawson 11.535
Bjellqvist 11.506
Wikipedia 11.989
Rodwell 11.286
Grimsley 11.579
Solomon 11.989
Lehninger 11.901
Nozaki 11.52
DTASelect 11.506
Thurlkill 11.52
EMBOSS 12.003
Sillero 11.52
Patrickios 10.994
IPC_peptide 11.989
IPC2_peptide 10.979
IPC2.peptide.svr19 9.195
Protein with the highest isoelectric point:
>tr|A0A0A0YUE0|A0A0A0YUE0_9CIRC Capsid OS=Bat circovirus POA/2012/VI OX=1572239 GN=Cap PE=4 SV=1
MM1 pKa = 7.38 SNSTVRR7 pKa = 11.84 RR8 pKa = 11.84 FCFTWNNYY16 pKa = 7.15 TVADD20 pKa = 4.1 TLTVKK25 pKa = 10.69 DD26 pKa = 6.02 FFIQHH31 pKa = 6.26 CKK33 pKa = 10.2 YY34 pKa = 10.51 GIAGEE39 pKa = 4.53 EE40 pKa = 3.96 IAPTTGTPHH49 pKa = 6.23 LQGFANLSKK58 pKa = 10.6 PMRR61 pKa = 11.84 FSTIKK66 pKa = 10.06 KK67 pKa = 8.07 HH68 pKa = 5.0 LHH70 pKa = 5.39 NGIHH74 pKa = 6.11 IEE76 pKa = 4.0 KK77 pKa = 10.66 ANGSDD82 pKa = 3.6 EE83 pKa = 4.48 QNQAYY88 pKa = 9.27 CKK90 pKa = 10.51 KK91 pKa = 9.91 SGTWFEE97 pKa = 4.06 QGTPCSQGSRR107 pKa = 11.84 SDD109 pKa = 3.53 LADD112 pKa = 3.38 VVSAIQNGTNTTQAIAQLYY131 pKa = 7.72 PIAYY135 pKa = 7.69 IKK137 pKa = 10.26 YY138 pKa = 7.88 YY139 pKa = 10.66 KK140 pKa = 10.33 GINEE144 pKa = 4.05 YY145 pKa = 11.01 LKK147 pKa = 9.42 IQHH150 pKa = 7.19 PITPRR155 pKa = 11.84 RR156 pKa = 11.84 HH157 pKa = 5.04 KK158 pKa = 9.46 TWVYY162 pKa = 9.69 YY163 pKa = 9.85 YY164 pKa = 9.15 WGPPGTGKK172 pKa = 10.03 SRR174 pKa = 11.84 RR175 pKa = 11.84 ALKK178 pKa = 10.01 KK179 pKa = 10.4 RR180 pKa = 11.84 RR181 pKa = 11.84 VSTQKK186 pKa = 10.48 VSTTSQGACGGTDD199 pKa = 3.19 TSNKK203 pKa = 7.89 RR204 pKa = 11.84 TSSSTTSTGGSNTTNYY220 pKa = 10.79 SKK222 pKa = 11.34 LLTDD226 pKa = 4.0 TLTKK230 pKa = 10.8 YY231 pKa = 10.43 KK232 pKa = 10.85
Molecular weight: 25.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.175
IPC2_protein 9.326
IPC_protein 9.238
Toseland 9.838
ProMoST 9.619
Dawson 10.087
Bjellqvist 9.794
Wikipedia 10.262
Rodwell 10.482
Grimsley 10.175
Solomon 10.101
Lehninger 10.058
Nozaki 9.897
DTASelect 9.765
Thurlkill 9.926
EMBOSS 10.248
Sillero 10.014
Patrickios 7.527
IPC_peptide 10.101
IPC2_peptide 8.551
IPC2.peptide.svr19 8.296
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
451
219
232
225.5
25.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.543 ± 0.043
1.552 ± 0.435
2.882 ± 0.097
2.661 ± 0.054
4.213 ± 0.862
6.208 ± 1.429
2.439 ± 0.417
5.543 ± 0.043
5.987 ± 1.899
5.765 ± 0.738
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.774 ± 0.657
5.987 ± 0.276
4.878 ± 0.72
4.656 ± 0.372
9.313 ± 3.296
6.874 ± 1.571
11.308 ± 1.481
6.208 ± 1.99
1.33 ± 0.284
4.878 ± 0.523
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here