Arthrobacter sp. MYb227

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3811 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S8WFT2|A0A2S8WFT2_9MICC Hydroxymethylpyrimidine kinase OS=Arthrobacter sp. MYb227 OX=1848601 GN=thiD PE=4 SV=1
MM1 pKa = 7.3SKK3 pKa = 10.28VLASLRR9 pKa = 11.84YY10 pKa = 8.65SAEE13 pKa = 4.14HH14 pKa = 6.38EE15 pKa = 4.33WLDD18 pKa = 3.66DD19 pKa = 3.51ATPAKK24 pKa = 10.71VGITQVAADD33 pKa = 3.75ALGDD37 pKa = 3.73VVYY40 pKa = 11.27VDD42 pKa = 4.35LPEE45 pKa = 5.2VGDD48 pKa = 3.71AVTAGEE54 pKa = 4.3TCGEE58 pKa = 4.21VEE60 pKa = 4.36STKK63 pKa = 10.7SVSDD67 pKa = 4.05LYY69 pKa = 11.64SPVTGTIVAVNQDD82 pKa = 4.42AIDD85 pKa = 3.86NPAILNEE92 pKa = 4.18DD93 pKa = 3.85PYY95 pKa = 11.17GAGWLFTVEE104 pKa = 4.19VTEE107 pKa = 4.42EE108 pKa = 4.66GPLLTAAEE116 pKa = 4.26YY117 pKa = 10.96ASANGGDD124 pKa = 3.8VAA126 pKa = 5.53

Molecular weight: 13.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S8WW95|A0A2S8WW95_9MICC ABC transporter ATP-binding protein OS=Arthrobacter sp. MYb227 OX=1848601 GN=CQ018_03555 PE=4 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.44KK7 pKa = 9.48RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.32RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3811

0

3811

1217330

32

1804

319.4

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.034 ± 0.055

0.64 ± 0.01

5.132 ± 0.032

5.935 ± 0.04

3.425 ± 0.025

8.493 ± 0.035

2.13 ± 0.019

5.415 ± 0.03

3.308 ± 0.033

10.487 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.019

2.909 ± 0.021

4.98 ± 0.026

3.243 ± 0.021

5.789 ± 0.035

6.474 ± 0.03

6.095 ± 0.028

7.731 ± 0.033

1.41 ± 0.016

2.079 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski