Fowl aviadenovirus B
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y5G377|A0A4Y5G377_9ADEN PVII OS=Fowl aviadenovirus B OX=190062 PE=4 SV=1
GG1 pKa = 6.54 QSAVSTGDD9 pKa = 3.16 EE10 pKa = 4.3 FFGDD14 pKa = 3.76 EE15 pKa = 3.81 VDD17 pKa = 4.39 FAARR21 pKa = 11.84 VTATVLADD29 pKa = 3.45 NDD31 pKa = 3.58 ASADD35 pKa = 3.94 CSCGLDD41 pKa = 3.24 TGKK44 pKa = 9.95 NVCSLLSCHH53 pKa = 6.71 CVILL57 pKa = 4.29
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 0.299
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.757
Protein with the highest isoelectric point:
>tr|A0A4Y5G379|A0A4Y5G379_9ADEN ORF57 OS=Fowl aviadenovirus B OX=190062 PE=4 SV=1
MM1 pKa = 7.59 SILVSPNDD9 pKa = 3.21 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SAMRR24 pKa = 11.84 GVGTRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PRR33 pKa = 11.84 RR34 pKa = 11.84 SRR36 pKa = 11.84 MTLRR40 pKa = 11.84 TLLGLGTASRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 GRR55 pKa = 11.84 RR56 pKa = 11.84 VGRR59 pKa = 11.84 RR60 pKa = 11.84 SRR62 pKa = 11.84 PAATSSRR69 pKa = 11.84 LVVVRR74 pKa = 11.84 TSRR77 pKa = 11.84 RR78 pKa = 11.84 SRR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 3.11
Molecular weight: 9.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.359
IPC_protein 12.969
Toseland 13.13
ProMoST 13.627
Dawson 13.13
Bjellqvist 13.13
Wikipedia 13.612
Rodwell 12.632
Grimsley 13.173
Solomon 13.627
Lehninger 13.525
Nozaki 13.13
DTASelect 13.13
Thurlkill 13.13
EMBOSS 13.627
Sillero 13.13
Patrickios 12.34
IPC_peptide 13.627
IPC2_peptide 12.618
IPC2.peptide.svr19 9.289
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
35
1
36
13173
33
1297
365.9
41.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.88 ± 0.385
1.928 ± 0.25
5.853 ± 0.225
6.065 ± 0.524
4.001 ± 0.216
6.566 ± 0.361
2.346 ± 0.18
3.917 ± 0.206
3.621 ± 0.316
8.768 ± 0.414
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.177
4.061 ± 0.436
6.604 ± 0.293
3.575 ± 0.205
7.576 ± 0.585
7.098 ± 0.405
5.982 ± 0.394
6.399 ± 0.279
1.45 ± 0.13
4.001 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here