Amino acid dipepetide frequency for Roseobacter phage CRP-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.623AlaAla: 10.623 ± 1.402
0.368AlaCys: 0.368 ± 0.192
6.57AlaAsp: 6.57 ± 0.762
7.614AlaGlu: 7.614 ± 0.957
2.763AlaPhe: 2.763 ± 0.371
8.289AlaGly: 8.289 ± 0.743
0.982AlaHis: 0.982 ± 0.249
4.728AlaIle: 4.728 ± 0.593
5.465AlaLys: 5.465 ± 0.674
7.798AlaLeu: 7.798 ± 1.083
3.009AlaMet: 3.009 ± 0.444
5.035AlaAsn: 5.035 ± 1.267
3.623AlaPro: 3.623 ± 0.532
4.114AlaGln: 4.114 ± 0.569
4.605AlaArg: 4.605 ± 0.691
6.017AlaSer: 6.017 ± 1.006
5.158AlaThr: 5.158 ± 1.001
6.324AlaVal: 6.324 ± 0.539
0.614AlaTrp: 0.614 ± 0.225
2.333AlaTyr: 2.333 ± 0.339
0.0AlaXaa: 0.0 ± 0.0
Cys
0.553CysAla: 0.553 ± 0.247
0.123CysCys: 0.123 ± 0.099
0.553CysAsp: 0.553 ± 0.192
0.614CysGlu: 0.614 ± 0.249
0.368CysPhe: 0.368 ± 0.191
1.044CysGly: 1.044 ± 0.371
0.246CysHis: 0.246 ± 0.144
0.368CysIle: 0.368 ± 0.187
0.368CysLys: 0.368 ± 0.203
0.307CysLeu: 0.307 ± 0.16
0.061CysMet: 0.061 ± 0.059
0.491CysAsn: 0.491 ± 0.165
0.491CysPro: 0.491 ± 0.178
0.246CysGln: 0.246 ± 0.134
0.43CysArg: 0.43 ± 0.175
0.307CysSer: 0.307 ± 0.158
0.553CysThr: 0.553 ± 0.226
0.246CysVal: 0.246 ± 0.155
0.184CysTrp: 0.184 ± 0.128
0.307CysTyr: 0.307 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
6.57AspAla: 6.57 ± 0.875
0.491AspCys: 0.491 ± 0.179
3.991AspAsp: 3.991 ± 0.53
4.851AspGlu: 4.851 ± 0.66
2.886AspPhe: 2.886 ± 0.344
5.526AspGly: 5.526 ± 0.774
0.921AspHis: 0.921 ± 0.314
4.114AspIle: 4.114 ± 0.528
4.114AspLys: 4.114 ± 0.577
5.895AspLeu: 5.895 ± 0.572
2.947AspMet: 2.947 ± 0.442
2.517AspAsn: 2.517 ± 0.333
3.009AspPro: 3.009 ± 0.562
3.193AspGln: 3.193 ± 0.541
3.316AspArg: 3.316 ± 0.39
3.07AspSer: 3.07 ± 0.413
4.36AspThr: 4.36 ± 0.485
4.36AspVal: 4.36 ± 0.575
1.044AspTrp: 1.044 ± 0.205
1.903AspTyr: 1.903 ± 0.402
0.0AspXaa: 0.0 ± 0.0
Glu
7.982GluAla: 7.982 ± 0.848
0.675GluCys: 0.675 ± 0.249
4.789GluAsp: 4.789 ± 0.584
5.403GluGlu: 5.403 ± 1.219
3.193GluPhe: 3.193 ± 0.466
4.175GluGly: 4.175 ± 0.644
0.737GluHis: 0.737 ± 0.181
3.439GluIle: 3.439 ± 0.416
3.684GluLys: 3.684 ± 0.472
5.035GluLeu: 5.035 ± 0.658
2.947GluMet: 2.947 ± 0.486
2.579GluAsn: 2.579 ± 0.388
2.64GluPro: 2.64 ± 0.388
2.64GluGln: 2.64 ± 0.592
3.07GluArg: 3.07 ± 0.606
2.21GluSer: 2.21 ± 0.372
4.175GluThr: 4.175 ± 0.473
5.035GluVal: 5.035 ± 0.703
0.86GluTrp: 0.86 ± 0.235
2.395GluTyr: 2.395 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
2.947PheAla: 2.947 ± 0.249
0.307PheCys: 0.307 ± 0.157
2.825PheAsp: 2.825 ± 0.448
2.763PheGlu: 2.763 ± 0.514
1.105PhePhe: 1.105 ± 0.326
4.175PheGly: 4.175 ± 0.736
0.614PheHis: 0.614 ± 0.173
1.412PheIle: 1.412 ± 0.287
2.026PheLys: 2.026 ± 0.394
2.21PheLeu: 2.21 ± 0.378
0.86PheMet: 0.86 ± 0.208
2.763PheAsn: 2.763 ± 0.382
1.658PhePro: 1.658 ± 0.367
1.167PheGln: 1.167 ± 0.237
2.088PheArg: 2.088 ± 0.357
2.763PheSer: 2.763 ± 0.421
2.702PheThr: 2.702 ± 0.399
1.719PheVal: 1.719 ± 0.348
0.246PheTrp: 0.246 ± 0.107
1.289PheTyr: 1.289 ± 0.313
0.0PheXaa: 0.0 ± 0.0
Gly
5.956GlyAla: 5.956 ± 0.604
0.737GlyCys: 0.737 ± 0.206
5.71GlyAsp: 5.71 ± 0.589
4.237GlyGlu: 4.237 ± 0.577
3.316GlyPhe: 3.316 ± 0.557
10.745GlyGly: 10.745 ± 3.333
0.86GlyHis: 0.86 ± 0.228
4.912GlyIle: 4.912 ± 0.439
3.684GlyLys: 3.684 ± 0.483
6.447GlyLeu: 6.447 ± 0.736
2.702GlyMet: 2.702 ± 0.543
4.482GlyAsn: 4.482 ± 0.755
2.21GlyPro: 2.21 ± 0.367
3.316GlyGln: 3.316 ± 0.424
3.93GlyArg: 3.93 ± 0.555
6.693GlySer: 6.693 ± 1.122
6.386GlyThr: 6.386 ± 1.129
5.158GlyVal: 5.158 ± 0.536
0.737GlyTrp: 0.737 ± 0.227
2.579GlyTyr: 2.579 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
0.86HisAla: 0.86 ± 0.27
0.307HisCys: 0.307 ± 0.122
1.105HisAsp: 1.105 ± 0.269
1.351HisGlu: 1.351 ± 0.38
0.246HisPhe: 0.246 ± 0.141
0.982HisGly: 0.982 ± 0.252
0.368HisHis: 0.368 ± 0.183
0.614HisIle: 0.614 ± 0.263
0.614HisLys: 0.614 ± 0.246
0.798HisLeu: 0.798 ± 0.262
0.491HisMet: 0.491 ± 0.209
0.798HisAsn: 0.798 ± 0.252
0.737HisPro: 0.737 ± 0.24
0.368HisGln: 0.368 ± 0.142
0.675HisArg: 0.675 ± 0.204
0.982HisSer: 0.982 ± 0.255
0.675HisThr: 0.675 ± 0.193
0.86HisVal: 0.86 ± 0.246
0.184HisTrp: 0.184 ± 0.132
0.491HisTyr: 0.491 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
5.158IleAla: 5.158 ± 0.578
0.491IleCys: 0.491 ± 0.157
4.789IleAsp: 4.789 ± 0.444
3.93IleGlu: 3.93 ± 0.674
1.474IlePhe: 1.474 ± 0.268
4.114IleGly: 4.114 ± 0.42
0.798IleHis: 0.798 ± 0.225
2.333IleIle: 2.333 ± 0.426
2.456IleLys: 2.456 ± 0.445
3.132IleLeu: 3.132 ± 0.524
0.86IleMet: 0.86 ± 0.203
3.009IleAsn: 3.009 ± 0.512
2.272IlePro: 2.272 ± 0.513
1.658IleGln: 1.658 ± 0.375
2.825IleArg: 2.825 ± 0.498
4.298IleSer: 4.298 ± 0.586
4.789IleThr: 4.789 ± 1.085
2.272IleVal: 2.272 ± 0.27
0.491IleTrp: 0.491 ± 0.177
1.351IleTyr: 1.351 ± 0.27
0.0IleXaa: 0.0 ± 0.0
Lys
5.956LysAla: 5.956 ± 0.667
0.43LysCys: 0.43 ± 0.24
4.114LysAsp: 4.114 ± 0.541
3.254LysGlu: 3.254 ± 0.439
2.456LysPhe: 2.456 ± 0.409
3.377LysGly: 3.377 ± 0.618
0.86LysHis: 0.86 ± 0.211
3.193LysIle: 3.193 ± 0.52
3.684LysLys: 3.684 ± 0.898
4.36LysLeu: 4.36 ± 0.488
1.842LysMet: 1.842 ± 0.405
2.395LysAsn: 2.395 ± 0.391
2.088LysPro: 2.088 ± 0.417
2.088LysGln: 2.088 ± 0.404
2.088LysArg: 2.088 ± 0.39
3.439LysSer: 3.439 ± 0.487
3.07LysThr: 3.07 ± 0.479
2.456LysVal: 2.456 ± 0.435
0.982LysTrp: 0.982 ± 0.284
1.781LysTyr: 1.781 ± 0.333
0.0LysXaa: 0.0 ± 0.0
Leu
7.982LeuAla: 7.982 ± 0.802
0.614LeuCys: 0.614 ± 0.21
5.772LeuAsp: 5.772 ± 0.554
5.342LeuGlu: 5.342 ± 0.774
2.21LeuPhe: 2.21 ± 0.273
6.324LeuGly: 6.324 ± 0.768
0.798LeuHis: 0.798 ± 0.281
3.254LeuIle: 3.254 ± 0.578
4.114LeuLys: 4.114 ± 0.584
5.342LeuLeu: 5.342 ± 0.742
1.965LeuMet: 1.965 ± 0.451
3.991LeuAsn: 3.991 ± 0.565
2.64LeuPro: 2.64 ± 0.343
2.702LeuGln: 2.702 ± 0.444
4.36LeuArg: 4.36 ± 0.627
5.526LeuSer: 5.526 ± 0.533
4.667LeuThr: 4.667 ± 0.783
4.114LeuVal: 4.114 ± 0.545
0.614LeuTrp: 0.614 ± 0.191
2.395LeuTyr: 2.395 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
3.868MetAla: 3.868 ± 0.485
0.246MetCys: 0.246 ± 0.114
1.719MetAsp: 1.719 ± 0.35
1.719MetGlu: 1.719 ± 0.37
0.798MetPhe: 0.798 ± 0.233
2.456MetGly: 2.456 ± 0.414
0.43MetHis: 0.43 ± 0.221
1.719MetIle: 1.719 ± 0.402
2.026MetLys: 2.026 ± 0.439
1.228MetLeu: 1.228 ± 0.309
0.921MetMet: 0.921 ± 0.265
1.658MetAsn: 1.658 ± 0.354
1.167MetPro: 1.167 ± 0.204
1.535MetGln: 1.535 ± 0.313
1.719MetArg: 1.719 ± 0.343
2.456MetSer: 2.456 ± 0.407
1.719MetThr: 1.719 ± 0.395
1.105MetVal: 1.105 ± 0.214
0.491MetTrp: 0.491 ± 0.199
0.368MetTyr: 0.368 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
5.219AsnAla: 5.219 ± 0.635
0.43AsnCys: 0.43 ± 0.159
2.395AsnAsp: 2.395 ± 0.451
2.456AsnGlu: 2.456 ± 0.46
1.965AsnPhe: 1.965 ± 0.27
3.377AsnGly: 3.377 ± 0.538
0.553AsnHis: 0.553 ± 0.273
3.254AsnIle: 3.254 ± 0.745
2.947AsnLys: 2.947 ± 0.39
4.175AsnLeu: 4.175 ± 0.545
1.412AsnMet: 1.412 ± 0.41
3.316AsnAsn: 3.316 ± 1.02
2.64AsnPro: 2.64 ± 0.404
2.149AsnGln: 2.149 ± 0.48
1.719AsnArg: 1.719 ± 0.346
3.561AsnSer: 3.561 ± 0.61
3.254AsnThr: 3.254 ± 0.42
3.623AsnVal: 3.623 ± 0.69
0.491AsnTrp: 0.491 ± 0.185
1.535AsnTyr: 1.535 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
3.193ProAla: 3.193 ± 0.529
0.184ProCys: 0.184 ± 0.112
2.886ProAsp: 2.886 ± 0.438
4.114ProGlu: 4.114 ± 0.699
1.719ProPhe: 1.719 ± 0.419
2.947ProGly: 2.947 ± 0.561
0.43ProHis: 0.43 ± 0.221
1.903ProIle: 1.903 ± 0.288
2.456ProLys: 2.456 ± 0.398
3.377ProLeu: 3.377 ± 0.561
0.553ProMet: 0.553 ± 0.172
2.026ProAsn: 2.026 ± 0.279
1.289ProPro: 1.289 ± 0.384
1.474ProGln: 1.474 ± 0.415
1.474ProArg: 1.474 ± 0.269
2.763ProSer: 2.763 ± 0.512
2.395ProThr: 2.395 ± 0.312
2.456ProVal: 2.456 ± 0.357
0.246ProTrp: 0.246 ± 0.114
1.289ProTyr: 1.289 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
4.175GlnAla: 4.175 ± 0.55
0.061GlnCys: 0.061 ± 0.067
1.781GlnAsp: 1.781 ± 0.36
3.009GlnGlu: 3.009 ± 0.429
1.903GlnPhe: 1.903 ± 0.418
2.702GlnGly: 2.702 ± 0.525
0.614GlnHis: 0.614 ± 0.191
2.825GlnIle: 2.825 ± 0.404
2.149GlnLys: 2.149 ± 0.374
4.175GlnLeu: 4.175 ± 0.612
1.228GlnMet: 1.228 ± 0.325
1.719GlnAsn: 1.719 ± 0.313
1.596GlnPro: 1.596 ± 0.31
1.596GlnGln: 1.596 ± 0.296
2.149GlnArg: 2.149 ± 0.397
1.965GlnSer: 1.965 ± 0.33
2.64GlnThr: 2.64 ± 0.453
2.456GlnVal: 2.456 ± 0.45
0.368GlnTrp: 0.368 ± 0.176
1.596GlnTyr: 1.596 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
3.684ArgAla: 3.684 ± 0.602
0.246ArgCys: 0.246 ± 0.13
3.623ArgAsp: 3.623 ± 0.503
3.254ArgGlu: 3.254 ± 0.532
2.395ArgPhe: 2.395 ± 0.388
4.237ArgGly: 4.237 ± 0.472
0.982ArgHis: 0.982 ± 0.286
2.64ArgIle: 2.64 ± 0.42
2.517ArgLys: 2.517 ± 0.395
3.991ArgLeu: 3.991 ± 0.418
1.351ArgMet: 1.351 ± 0.271
2.456ArgAsn: 2.456 ± 0.405
1.719ArgPro: 1.719 ± 0.326
2.456ArgGln: 2.456 ± 0.336
3.623ArgArg: 3.623 ± 0.581
2.886ArgSer: 2.886 ± 0.389
3.009ArgThr: 3.009 ± 0.457
2.825ArgVal: 2.825 ± 0.482
0.86ArgTrp: 0.86 ± 0.211
1.289ArgTyr: 1.289 ± 0.33
0.0ArgXaa: 0.0 ± 0.0
Ser
5.219SerAla: 5.219 ± 0.982
0.675SerCys: 0.675 ± 0.207
4.851SerAsp: 4.851 ± 0.559
4.482SerGlu: 4.482 ± 0.625
2.456SerPhe: 2.456 ± 0.408
6.877SerGly: 6.877 ± 1.027
0.737SerHis: 0.737 ± 0.281
4.114SerIle: 4.114 ± 0.614
2.886SerLys: 2.886 ± 0.38
3.684SerLeu: 3.684 ± 0.526
1.596SerMet: 1.596 ± 0.368
3.377SerAsn: 3.377 ± 0.479
2.825SerPro: 2.825 ± 0.406
2.886SerGln: 2.886 ± 0.465
3.623SerArg: 3.623 ± 0.663
4.175SerSer: 4.175 ± 0.654
4.544SerThr: 4.544 ± 0.753
4.544SerVal: 4.544 ± 0.633
0.491SerTrp: 0.491 ± 0.165
1.658SerTyr: 1.658 ± 0.391
0.0SerXaa: 0.0 ± 0.0
Thr
6.14ThrAla: 6.14 ± 0.93
0.123ThrCys: 0.123 ± 0.084
3.991ThrAsp: 3.991 ± 0.472
3.439ThrGlu: 3.439 ± 0.461
2.21ThrPhe: 2.21 ± 0.43
6.816ThrGly: 6.816 ± 1.312
0.614ThrHis: 0.614 ± 0.179
3.07ThrIle: 3.07 ± 0.56
3.07ThrLys: 3.07 ± 0.503
4.789ThrLeu: 4.789 ± 0.547
1.596ThrMet: 1.596 ± 0.305
3.868ThrAsn: 3.868 ± 0.75
3.193ThrPro: 3.193 ± 0.536
3.193ThrGln: 3.193 ± 0.4
3.132ThrArg: 3.132 ± 0.368
5.035ThrSer: 5.035 ± 0.557
4.36ThrThr: 4.36 ± 0.723
4.789ThrVal: 4.789 ± 0.919
0.491ThrTrp: 0.491 ± 0.182
1.719ThrTyr: 1.719 ± 0.329
0.0ThrXaa: 0.0 ± 0.0
Val
6.693ValAla: 6.693 ± 1.034
0.921ValCys: 0.921 ± 0.322
4.482ValAsp: 4.482 ± 0.447
3.193ValGlu: 3.193 ± 0.391
2.886ValPhe: 2.886 ± 0.685
3.807ValGly: 3.807 ± 0.488
0.737ValHis: 0.737 ± 0.271
3.009ValIle: 3.009 ± 0.437
2.886ValLys: 2.886 ± 0.514
4.544ValLeu: 4.544 ± 0.417
1.781ValMet: 1.781 ± 0.323
2.579ValAsn: 2.579 ± 0.48
2.088ValPro: 2.088 ± 0.344
2.149ValGln: 2.149 ± 0.288
2.947ValArg: 2.947 ± 0.417
4.298ValSer: 4.298 ± 0.509
4.667ValThr: 4.667 ± 0.972
3.316ValVal: 3.316 ± 0.407
0.675ValTrp: 0.675 ± 0.196
2.456ValTyr: 2.456 ± 0.397
0.0ValXaa: 0.0 ± 0.0
Trp
1.351TrpAla: 1.351 ± 0.31
0.061TrpCys: 0.061 ± 0.073
0.614TrpAsp: 0.614 ± 0.188
0.43TrpGlu: 0.43 ± 0.153
0.307TrpPhe: 0.307 ± 0.143
0.675TrpGly: 0.675 ± 0.212
0.553TrpHis: 0.553 ± 0.188
0.246TrpIle: 0.246 ± 0.143
0.553TrpLys: 0.553 ± 0.197
0.982TrpLeu: 0.982 ± 0.268
0.491TrpMet: 0.491 ± 0.151
0.368TrpAsn: 0.368 ± 0.156
0.368TrpPro: 0.368 ± 0.157
0.43TrpGln: 0.43 ± 0.159
0.553TrpArg: 0.553 ± 0.195
0.737TrpSer: 0.737 ± 0.205
0.553TrpThr: 0.553 ± 0.144
0.737TrpVal: 0.737 ± 0.227
0.061TrpTrp: 0.061 ± 0.073
0.614TrpTyr: 0.614 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.903TyrAla: 1.903 ± 0.325
0.368TyrCys: 0.368 ± 0.157
2.517TyrAsp: 2.517 ± 0.404
2.21TyrGlu: 2.21 ± 0.323
0.982TyrPhe: 0.982 ± 0.24
2.149TyrGly: 2.149 ± 0.378
0.675TyrHis: 0.675 ± 0.207
1.351TyrIle: 1.351 ± 0.25
2.21TyrLys: 2.21 ± 0.423
2.456TyrLeu: 2.456 ± 0.359
0.675TyrMet: 0.675 ± 0.194
1.044TyrAsn: 1.044 ± 0.25
0.921TyrPro: 0.921 ± 0.267
1.351TyrGln: 1.351 ± 0.295
1.719TyrArg: 1.719 ± 0.324
2.579TyrSer: 2.579 ± 0.39
1.965TyrThr: 1.965 ± 0.407
1.719TyrVal: 1.719 ± 0.249
0.553TyrTrp: 0.553 ± 0.206
1.044TyrTyr: 1.044 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (16287 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski