Staphylococcus virus X2
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q4ZA47|Q4ZA47_9CAUD ORF036 OS=Staphylococcus virus X2 OX=320850 PE=4 SV=1
MM1 pKa = 6.89 QQQAYY6 pKa = 9.38 INATIDD12 pKa = 2.95 IRR14 pKa = 11.84 IPTEE18 pKa = 3.45 VEE20 pKa = 3.82 YY21 pKa = 10.92 QHH23 pKa = 6.88 FDD25 pKa = 3.52 DD26 pKa = 4.52 VDD28 pKa = 3.67 KK29 pKa = 11.4 EE30 pKa = 4.28 KK31 pKa = 10.38 EE32 pKa = 4.13 TLADD36 pKa = 3.57 YY37 pKa = 10.81 LYY39 pKa = 10.9 NNPDD43 pKa = 3.71 EE44 pKa = 4.28 ILEE47 pKa = 3.92 YY48 pKa = 11.25 DD49 pKa = 3.43 NLKK52 pKa = 9.69 IRR54 pKa = 11.84 NVNVEE59 pKa = 3.93 VEE61 pKa = 4.1
Molecular weight: 7.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.068
IPC2_protein 4.075
IPC_protein 3.973
Toseland 3.795
ProMoST 4.062
Dawson 3.948
Bjellqvist 4.177
Wikipedia 3.859
Rodwell 3.808
Grimsley 3.706
Solomon 3.935
Lehninger 3.884
Nozaki 4.075
DTASelect 4.228
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.088
Patrickios 3.032
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 4.019
Protein with the highest isoelectric point:
>tr|Q4ZA90|Q4ZA90_9CAUD ORF003 OS=Staphylococcus virus X2 OX=320850 PE=4 SV=1
MM1 pKa = 6.82 MQVSCIGTRR10 pKa = 11.84 VIRR13 pKa = 11.84 IRR15 pKa = 11.84 LILITYY21 pKa = 9.62 KK22 pKa = 10.89 DD23 pKa = 3.61 STSKK27 pKa = 9.83 QRR29 pKa = 11.84 NYY31 pKa = 10.33 CLNVVSII38 pKa = 4.61
Molecular weight: 4.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.545
IPC_protein 10.014
Toseland 10.028
ProMoST 9.838
Dawson 10.277
Bjellqvist 10.058
Wikipedia 10.482
Rodwell 10.511
Grimsley 10.365
Solomon 10.365
Lehninger 10.335
Nozaki 10.16
DTASelect 10.014
Thurlkill 10.131
EMBOSS 10.452
Sillero 10.218
Patrickios 10.423
IPC_peptide 10.35
IPC2_peptide 9.355
IPC2.peptide.svr19 8.259
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
77
0
77
13954
33
989
181.2
20.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.54 ± 0.348
0.545 ± 0.097
6.034 ± 0.222
7.374 ± 0.478
4.221 ± 0.17
5.532 ± 0.381
1.713 ± 0.113
7.41 ± 0.234
8.951 ± 0.397
7.453 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.687 ± 0.19
6.443 ± 0.251
2.852 ± 0.189
4.178 ± 0.225
4.25 ± 0.262
6.07 ± 0.256
6.5 ± 0.283
6.5 ± 0.308
1.225 ± 0.154
4.522 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here