bat polyomavirus 5b1
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5ZZ30|A0A0D5ZZ30_9POLY Minor capsid protein OS=bat polyomavirus 5b1 OX=2758139 GN=VP2 PE=3 SV=1
MM1 pKa = 7.54 GGFISLLLNLAEE13 pKa = 4.86 ALPEE17 pKa = 4.02 LSAATGFSVEE27 pKa = 4.58 AIISGEE33 pKa = 3.55 AAAAIEE39 pKa = 5.39 AEE41 pKa = 4.45 ASWLMSSTGLALTAAEE57 pKa = 4.22 ALEE60 pKa = 4.23 ATGITAEE67 pKa = 4.32 SISMLFAVPSALRR80 pKa = 11.84 DD81 pKa = 3.66 LVGLGITFQTVTGVSALVAAGIRR104 pKa = 11.84 LEE106 pKa = 3.9 QHH108 pKa = 4.86 QVSVVNQNNMALSIWRR124 pKa = 11.84 PQDD127 pKa = 3.2 YY128 pKa = 11.08 YY129 pKa = 11.42 DD130 pKa = 3.73 ILFPGVNSFVQAINVVEE147 pKa = 4.08 NWGSSLFNYY156 pKa = 9.62 LGRR159 pKa = 11.84 YY160 pKa = 8.17 IWDD163 pKa = 3.48 SLVRR167 pKa = 11.84 EE168 pKa = 4.24 ARR170 pKa = 11.84 LQIGQATNEE179 pKa = 4.04 LAVRR183 pKa = 11.84 AHH185 pKa = 6.52 HH186 pKa = 6.33 SFHH189 pKa = 7.63 DD190 pKa = 3.59 ALARR194 pKa = 11.84 VAEE197 pKa = 4.15 NTRR200 pKa = 11.84 WVITNGPANAYY211 pKa = 9.42 SALADD216 pKa = 3.65 YY217 pKa = 10.58 YY218 pKa = 11.64 GRR220 pKa = 11.84 LPPLNPAQVRR230 pKa = 11.84 QLEE233 pKa = 4.24 RR234 pKa = 11.84 RR235 pKa = 11.84 IEE237 pKa = 4.08 APPRR241 pKa = 11.84 NRR243 pKa = 11.84 RR244 pKa = 11.84 AIEE247 pKa = 3.95 EE248 pKa = 4.22 EE249 pKa = 4.37 DD250 pKa = 3.37 PHH252 pKa = 8.64 SSGEE256 pKa = 4.02 VVEE259 pKa = 5.58 RR260 pKa = 11.84 YY261 pKa = 7.52 EE262 pKa = 4.37 APGGAAQRR270 pKa = 11.84 ITPDD274 pKa = 2.47 WMLPLILGLYY284 pKa = 10.46 GDD286 pKa = 4.56 ITPAWGAEE294 pKa = 3.71 IEE296 pKa = 4.49 KK297 pKa = 10.18 IEE299 pKa = 4.9 EE300 pKa = 3.57 EE301 pKa = 4.49 DD302 pKa = 3.85 GPKK305 pKa = 10.07 KK306 pKa = 10.09 KK307 pKa = 9.97 RR308 pKa = 11.84 RR309 pKa = 11.84 RR310 pKa = 11.84 LL311 pKa = 3.4
Molecular weight: 33.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.958
IPC2_protein 4.965
IPC_protein 4.876
Toseland 4.787
ProMoST 4.978
Dawson 4.825
Bjellqvist 4.965
Wikipedia 4.647
Rodwell 4.749
Grimsley 4.698
Solomon 4.825
Lehninger 4.774
Nozaki 4.94
DTASelect 5.016
Thurlkill 4.774
EMBOSS 4.685
Sillero 5.016
Patrickios 4.113
IPC_peptide 4.838
IPC2_peptide 5.016
IPC2.peptide.svr19 4.966
Protein with the highest isoelectric point:
>tr|A0A0D5ZZ30|A0A0D5ZZ30_9POLY Minor capsid protein OS=bat polyomavirus 5b1 OX=2758139 GN=VP2 PE=3 SV=1
MM1 pKa = 7.37 APKK4 pKa = 10.43 RR5 pKa = 11.84 KK6 pKa = 10.27 GGDD9 pKa = 3.29 SKK11 pKa = 11.69 GEE13 pKa = 3.97 KK14 pKa = 9.85 SCSTKK19 pKa = 10.3 CSPKK23 pKa = 10.39 ACPTVNPVPKK33 pKa = 10.2 LIVKK37 pKa = 10.44 GGVQVLSVRR46 pKa = 11.84 TGPDD50 pKa = 3.06 SITQIEE56 pKa = 4.95 AYY58 pKa = 10.14 LNPRR62 pKa = 11.84 MGTLADD68 pKa = 3.64 TSKK71 pKa = 8.12 MTGYY75 pKa = 10.93 SMPVGVATSWSSDD88 pKa = 3.33 NPKK91 pKa = 10.46 AEE93 pKa = 4.13 HH94 pKa = 6.62 LPTYY98 pKa = 9.72 SCARR102 pKa = 11.84 IALPVLNEE110 pKa = 4.7 DD111 pKa = 4.08 LTCDD115 pKa = 3.58 TLQMWEE121 pKa = 4.04 AVSVKK126 pKa = 9.75 TEE128 pKa = 4.02 VVGAPSLTDD137 pKa = 2.63 VHH139 pKa = 6.84 FYY141 pKa = 10.1 AKK143 pKa = 10.47 KK144 pKa = 9.17 MYY146 pKa = 9.46 DD147 pKa = 3.32 GKK149 pKa = 11.2 GIAIPIEE156 pKa = 4.11 GMAMHH161 pKa = 6.75 FFAVGGEE168 pKa = 3.94 PLDD171 pKa = 3.77 LQGIVANGQAKK182 pKa = 9.66 YY183 pKa = 9.76 PEE185 pKa = 4.53 GMVSIPGMTHH195 pKa = 7.09 LNQVLQPSAKK205 pKa = 10.36 AKK207 pKa = 8.4 LTKK210 pKa = 10.56 DD211 pKa = 2.75 GAYY214 pKa = 9.2 PVEE217 pKa = 4.54 VWSPDD222 pKa = 3.29 PAKK225 pKa = 10.96 NEE227 pKa = 3.57 NTRR230 pKa = 11.84 YY231 pKa = 9.81 FGTYY235 pKa = 9.05 TGGQTTPPVLQFTNTVTTVLLDD257 pKa = 3.84 EE258 pKa = 5.12 NGVGPLCKK266 pKa = 10.08 GDD268 pKa = 4.65 GLFLSCVDD276 pKa = 2.84 ICGFLVDD283 pKa = 5.6 GYY285 pKa = 8.72 TSQMSFRR292 pKa = 11.84 GLPRR296 pKa = 11.84 YY297 pKa = 10.0 FNVTLRR303 pKa = 11.84 KK304 pKa = 9.9 RR305 pKa = 11.84 NVKK308 pKa = 8.72 TLTLL312 pKa = 3.76
Molecular weight: 33.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.247
IPC2_protein 7.571
IPC_protein 7.454
Toseland 7.015
ProMoST 8.009
Dawson 8.156
Bjellqvist 8.521
Wikipedia 8.068
Rodwell 8.17
Grimsley 7.015
Solomon 8.244
Lehninger 8.258
Nozaki 8.756
DTASelect 8.244
Thurlkill 8.302
EMBOSS 8.375
Sillero 8.639
Patrickios 4.139
IPC_peptide 8.229
IPC2_peptide 7.483
IPC2.peptide.svr19 7.656
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1437
188
626
359.3
40.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.029 ± 1.887
2.714 ± 0.967
5.219 ± 0.799
6.472 ± 0.918
4.384 ± 0.841
6.333 ± 1.229
2.227 ± 0.409
4.941 ± 0.661
7.307 ± 1.756
9.812 ± 0.452
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.132 ± 0.483
4.384 ± 0.238
5.776 ± 0.597
4.036 ± 0.411
3.897 ± 0.772
5.846 ± 0.661
5.428 ± 1.077
6.124 ± 1.014
1.531 ± 0.6
3.41 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here