Magnaporthe oryzae virus 1
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q60GI3|Q60GI3_9VIRU Putative coat protein OS=Magnaporthe oryzae virus 1 OX=271257 PE=4 SV=1
MM1 pKa = 7.18 AQIGAPNFLSSVLGDD16 pKa = 3.38 QRR18 pKa = 11.84 GGVLNSDD25 pKa = 3.67 SVFRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.22 RR32 pKa = 11.84 AAITTSTIVRR42 pKa = 11.84 GVQDD46 pKa = 3.41 TRR48 pKa = 11.84 VGFLVFAVGRR58 pKa = 11.84 VFSSFSAALVRR69 pKa = 11.84 PKK71 pKa = 10.85 SSVPAIDD78 pKa = 3.32 ATYY81 pKa = 8.75 PCPATRR87 pKa = 11.84 SEE89 pKa = 4.4 EE90 pKa = 4.11 FVGLAKK96 pKa = 10.32 KK97 pKa = 10.47 YY98 pKa = 10.76 SNFSSTFTHH107 pKa = 6.79 ANFAGVVEE115 pKa = 4.22 RR116 pKa = 11.84 LSRR119 pKa = 11.84 ALAVLSTLPDD129 pKa = 3.42 SDD131 pKa = 3.95 TAALTSLDD139 pKa = 3.22 IAGGVMPTVYY149 pKa = 10.55 SIATFDD155 pKa = 3.86 SPVNALARR163 pKa = 11.84 VVFIPRR169 pKa = 11.84 IVDD172 pKa = 3.5 SLLSPNSLAVLIAAVAGEE190 pKa = 4.43 GSSVATDD197 pKa = 3.42 ILRR200 pKa = 11.84 LDD202 pKa = 3.35 VSTRR206 pKa = 11.84 KK207 pKa = 10.22 AIVPIVSGPGLATAIVDD224 pKa = 3.99 ALRR227 pKa = 11.84 VLASNMVEE235 pKa = 3.92 AGQGQLFSFAVTRR248 pKa = 11.84 GLHH251 pKa = 4.48 QALNVSSGTDD261 pKa = 3.31 GQYY264 pKa = 11.57 VMQSILRR271 pKa = 11.84 SGRR274 pKa = 11.84 FSPPLGGIHH283 pKa = 6.52 TSLPVFSGLPALVSEE298 pKa = 4.53 SRR300 pKa = 11.84 RR301 pKa = 11.84 DD302 pKa = 3.25 VATFVDD308 pKa = 4.36 ALALVSAAGAAPADD322 pKa = 3.62 PCIVEE327 pKa = 4.51 RR328 pKa = 11.84 GNTFPTVLVSPGQISDD344 pKa = 4.3 LDD346 pKa = 4.16 SSSSMPGAFPSTPQLLGQLKK366 pKa = 9.23 EE367 pKa = 3.91 ALPAFFSNYY376 pKa = 9.25 LRR378 pKa = 11.84 ILSTVFAAGEE388 pKa = 4.14 SDD390 pKa = 5.53 SVALDD395 pKa = 3.72 CACAFASLLEE405 pKa = 4.53 DD406 pKa = 4.15 LPTPLSAPTVLPFFWVEE423 pKa = 3.93 PTSLLPASVFSTTAEE438 pKa = 4.11 SAGFGALATPGSPVTRR454 pKa = 11.84 PAWGAIEE461 pKa = 4.86 HH462 pKa = 6.22 LVSPSSAVSTATVSWTSARR481 pKa = 11.84 QNPFLWHH488 pKa = 5.69 FKK490 pKa = 10.27 GMRR493 pKa = 11.84 SDD495 pKa = 3.57 PLAAVIVDD503 pKa = 3.69 QFDD506 pKa = 3.72 PTDD509 pKa = 3.48 LAFAGPGGAEE519 pKa = 3.84 TTLQKK524 pKa = 9.89 WGRR527 pKa = 11.84 QAPLTDD533 pKa = 3.68 YY534 pKa = 10.92 LWRR537 pKa = 11.84 DD538 pKa = 3.42 SASCLPAPGEE548 pKa = 4.0 LLNLSGAIGLRR559 pKa = 11.84 FRR561 pKa = 11.84 HH562 pKa = 5.95 FVIDD566 pKa = 5.25 DD567 pKa = 3.81 NDD569 pKa = 3.55 DD570 pKa = 3.58 CAFTRR575 pKa = 11.84 IPMFHH580 pKa = 7.4 EE581 pKa = 4.6 IGTGSVTIAVSRR593 pKa = 11.84 PCGLAPDD600 pKa = 5.05 GPPARR605 pKa = 11.84 LEE607 pKa = 4.0 RR608 pKa = 11.84 ATVVSPALGYY618 pKa = 10.33 LRR620 pKa = 11.84 QRR622 pKa = 11.84 GANLRR627 pKa = 11.84 AFGRR631 pKa = 11.84 PDD633 pKa = 3.8 DD634 pKa = 4.36 LLAPRR639 pKa = 11.84 RR640 pKa = 11.84 LGPASLTVAPKK651 pKa = 10.54 APPAPVSAPVSSFAFSGPKK670 pKa = 9.08 TSSRR674 pKa = 11.84 DD675 pKa = 3.62 PLSGEE680 pKa = 4.14 LPTPLVPVQHH690 pKa = 6.38 YY691 pKa = 9.74 KK692 pKa = 11.03 AEE694 pKa = 4.42 TGPKK698 pKa = 9.27 TGGLGAAGGGGGTVKK713 pKa = 10.43 PPTAVPPGLTEE724 pKa = 4.37 VPTAPDD730 pKa = 3.61 AGPTPEE736 pKa = 4.16 PHH738 pKa = 6.31 GAAPSLHH745 pKa = 6.22 EE746 pKa = 4.14
Molecular weight: 77.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.865
IPC2_protein 5.779
IPC_protein 5.83
Toseland 6.186
ProMoST 6.059
Dawson 6.097
Bjellqvist 6.109
Wikipedia 6.097
Rodwell 6.071
Grimsley 6.326
Solomon 6.097
Lehninger 6.084
Nozaki 6.351
DTASelect 6.547
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.453
Patrickios 4.457
IPC_peptide 6.109
IPC2_peptide 6.453
IPC2.peptide.svr19 6.392
Protein with the highest isoelectric point:
>tr|Q60GI3|Q60GI3_9VIRU Putative coat protein OS=Magnaporthe oryzae virus 1 OX=271257 PE=4 SV=1
MM1 pKa = 7.69 DD2 pKa = 5.05 LEE4 pKa = 4.55 ARR6 pKa = 11.84 SKK8 pKa = 10.93 SFGRR12 pKa = 11.84 LGMSCSSIYY21 pKa = 10.65 SKK23 pKa = 11.19 YY24 pKa = 9.88 RR25 pKa = 11.84 DD26 pKa = 3.87 HH27 pKa = 7.49 LPVFSSMDD35 pKa = 3.56 FLSGLTYY42 pKa = 9.28 VTTNVGVLRR51 pKa = 11.84 SHH53 pKa = 7.18 HH54 pKa = 6.85 PLLPTAVSLLCLDD67 pKa = 4.93 FPIQFDD73 pKa = 3.88 TSSSHH78 pKa = 6.92 CLLLAQSAYY87 pKa = 7.27 MTVDD91 pKa = 3.37 EE92 pKa = 5.1 LARR95 pKa = 11.84 SYY97 pKa = 10.44 PCKK100 pKa = 10.35 FGGRR104 pKa = 11.84 RR105 pKa = 11.84 ILGRR109 pKa = 11.84 LIHH112 pKa = 7.31 DD113 pKa = 3.64 STFRR117 pKa = 11.84 EE118 pKa = 3.85 ATFPVKK124 pKa = 10.31 SHH126 pKa = 6.99 PAAHH130 pKa = 6.09 TKK132 pKa = 10.57 AHH134 pKa = 6.54 ISLGALLRR142 pKa = 11.84 SWPSVAPRR150 pKa = 11.84 SEE152 pKa = 3.23 IDD154 pKa = 3.69 YY155 pKa = 10.95 ILARR159 pKa = 11.84 CAGRR163 pKa = 11.84 LTADD167 pKa = 3.16 QAIALIVYY175 pKa = 8.56 HH176 pKa = 6.41 VSLRR180 pKa = 11.84 PTFGLSSARR189 pKa = 11.84 FAVAALMCPGDD200 pKa = 4.35 AKK202 pKa = 11.07 GLSNALKK209 pKa = 10.48 ALGLNGVKK217 pKa = 10.17 EE218 pKa = 4.16 GAVLVEE224 pKa = 4.0 AQTLQGRR231 pKa = 11.84 GVAPIDD237 pKa = 3.38 WGRR240 pKa = 11.84 EE241 pKa = 3.73 IPSRR245 pKa = 11.84 CTDD248 pKa = 3.06 AVHH251 pKa = 6.62 EE252 pKa = 4.29 NTVFIPEE259 pKa = 4.02 ADD261 pKa = 3.39 LRR263 pKa = 11.84 AHH265 pKa = 5.77 VRR267 pKa = 11.84 HH268 pKa = 5.91 FLSSEE273 pKa = 3.85 LDD275 pKa = 3.17 ADD277 pKa = 4.13 TSLEE281 pKa = 4.21 PLDD284 pKa = 4.31 HH285 pKa = 5.7 WWSRR289 pKa = 11.84 RR290 pKa = 11.84 WAWCVNGAHH299 pKa = 6.4 TSAASRR305 pKa = 11.84 ALGIDD310 pKa = 3.22 HH311 pKa = 7.0 RR312 pKa = 11.84 HH313 pKa = 6.07 AFPAHH318 pKa = 5.17 SRR320 pKa = 11.84 VYY322 pKa = 10.73 RR323 pKa = 11.84 RR324 pKa = 11.84 MASEE328 pKa = 3.98 ALEE331 pKa = 4.2 SEE333 pKa = 4.87 PVSKK337 pKa = 10.24 WDD339 pKa = 3.06 GTTFVSASEE348 pKa = 4.02 KK349 pKa = 10.7 LEE351 pKa = 4.25 HH352 pKa = 6.45 GKK354 pKa = 7.98 TRR356 pKa = 11.84 AIFACDD362 pKa = 3.15 TRR364 pKa = 11.84 SYY366 pKa = 10.37 FAWSWLLDD374 pKa = 3.46 PVAANWRR381 pKa = 11.84 NSRR384 pKa = 11.84 ILLDD388 pKa = 3.81 PGRR391 pKa = 11.84 GGTYY395 pKa = 9.99 GSTRR399 pKa = 11.84 RR400 pKa = 11.84 IQNAQLTGGVNVMLDD415 pKa = 3.43 YY416 pKa = 11.58 DD417 pKa = 4.55 DD418 pKa = 6.12 FNSQHH423 pKa = 5.82 STRR426 pKa = 11.84 SMQIVTEE433 pKa = 4.19 EE434 pKa = 3.98 LCSYY438 pKa = 10.37 IGAPQWYY445 pKa = 8.19 TDD447 pKa = 3.79 VLVKK451 pKa = 10.81 SLDD454 pKa = 3.45 SEE456 pKa = 5.17 YY457 pKa = 9.72 ITGHH461 pKa = 5.54 GPPRR465 pKa = 11.84 HH466 pKa = 5.19 VAGTLMSGHH475 pKa = 7.16 RR476 pKa = 11.84 GTTFFNSILNGVYY489 pKa = 9.45 IRR491 pKa = 11.84 HH492 pKa = 6.27 AFGAGAFDD500 pKa = 4.15 SCVSMHH506 pKa = 6.58 TGDD509 pKa = 3.88 DD510 pKa = 3.25 VYY512 pKa = 11.08 MRR514 pKa = 11.84 LRR516 pKa = 11.84 TLRR519 pKa = 11.84 DD520 pKa = 3.05 ASTLLVSLKK529 pKa = 10.93 DD530 pKa = 3.3 LGCRR534 pKa = 11.84 LNPTKK539 pKa = 10.55 QSIGYY544 pKa = 8.49 KK545 pKa = 8.88 HH546 pKa = 6.75 AEE548 pKa = 4.12 FLRR551 pKa = 11.84 VAITPTGSRR560 pKa = 11.84 GYY562 pKa = 10.19 AARR565 pKa = 11.84 SIAALASGNWTDD577 pKa = 4.49 SDD579 pKa = 4.15 PMDD582 pKa = 4.2 ARR584 pKa = 11.84 DD585 pKa = 4.11 GFTTTLANIRR595 pKa = 11.84 TIQLRR600 pKa = 11.84 SGSPDD605 pKa = 3.02 LGRR608 pKa = 11.84 LVGPAVKK615 pKa = 10.11 YY616 pKa = 10.89 ARR618 pKa = 11.84 GLRR621 pKa = 11.84 TRR623 pKa = 11.84 TVIKK627 pKa = 10.07 ILNGEE632 pKa = 3.99 EE633 pKa = 4.49 SYY635 pKa = 11.35 NGSPVYY641 pKa = 10.07 NVPGRR646 pKa = 11.84 TVKK649 pKa = 10.24 VHH651 pKa = 5.82 TFAPKK656 pKa = 10.61 DD657 pKa = 3.63 PDD659 pKa = 3.57 YY660 pKa = 9.72 MLPEE664 pKa = 4.9 IPVSSSWPLNSTYY677 pKa = 10.86 DD678 pKa = 3.88 YY679 pKa = 11.33 LNSHH683 pKa = 5.92 CTGLEE688 pKa = 3.73 SRR690 pKa = 11.84 LFEE693 pKa = 4.19 IAQVSPVPLLAASSYY708 pKa = 11.33 SKK710 pKa = 11.14 GLAQSRR716 pKa = 11.84 QVLTSADD723 pKa = 3.51 LTIRR727 pKa = 11.84 PLGTVSTLGVIDD739 pKa = 4.1 VGSLSAAPPSPPVLAGHH756 pKa = 6.88 PLARR760 pKa = 11.84 LVAARR765 pKa = 11.84 VSDD768 pKa = 3.89 DD769 pKa = 3.8 DD770 pKa = 4.15 LRR772 pKa = 11.84 EE773 pKa = 4.04 ILDD776 pKa = 3.57 QFAIPPPPADD786 pKa = 3.27 MTVRR790 pKa = 11.84 EE791 pKa = 4.2 YY792 pKa = 11.44 AFGYY796 pKa = 9.73 EE797 pKa = 3.87 PHH799 pKa = 6.65 CVNVRR804 pKa = 11.84 GFLPHH809 pKa = 7.27 ADD811 pKa = 3.55 ASSLSARR818 pKa = 11.84 TRR820 pKa = 11.84 AGTIFVPTPVNVV832 pKa = 3.25
Molecular weight: 90.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.529
IPC2_protein 7.805
IPC_protein 7.629
Toseland 7.337
ProMoST 8.2
Dawson 8.302
Bjellqvist 8.712
Wikipedia 8.229
Rodwell 8.317
Grimsley 7.439
Solomon 8.361
Lehninger 8.375
Nozaki 8.843
DTASelect 8.419
Thurlkill 8.463
EMBOSS 8.507
Sillero 8.785
Patrickios 4.317
IPC_peptide 8.346
IPC2_peptide 7.878
IPC2.peptide.svr19 7.833
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1578
746
832
789.0
84.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.217 ± 1.029
1.331 ± 0.266
5.26 ± 0.294
3.169 ± 0.149
4.246 ± 0.575
7.858 ± 0.489
2.535 ± 0.81
3.802 ± 0.306
2.028 ± 0.284
10.266 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.331 ± 0.266
2.281 ± 0.274
7.668 ± 1.254
2.091 ± 0.127
6.591 ± 0.742
10.33 ± 0.176
6.781 ± 0.129
7.731 ± 0.666
1.204 ± 0.271
2.281 ± 0.729
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here