Dysgonomonas alginatilytica
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4322 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V3PRK5|A0A2V3PRK5_9BACT Uncharacterized protein OS=Dysgonomonas alginatilytica OX=1605892 GN=CLV62_10813 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.21 ISPNKK7 pKa = 9.62 FVSLSYY13 pKa = 10.8 DD14 pKa = 3.62 LNVGEE19 pKa = 4.67 GDD21 pKa = 3.42 EE22 pKa = 4.38 KK23 pKa = 11.42 EE24 pKa = 4.5 LMEE27 pKa = 4.92 KK28 pKa = 9.17 ATPEE32 pKa = 3.81 QPLEE36 pKa = 4.37 FIFGTNSMLEE46 pKa = 4.03 AFEE49 pKa = 4.87 KK50 pKa = 11.02 NVDD53 pKa = 3.51 GLAEE57 pKa = 4.37 GDD59 pKa = 3.78 SFDD62 pKa = 6.45 FILTPDD68 pKa = 3.47 QAYY71 pKa = 10.41 GEE73 pKa = 4.48 YY74 pKa = 10.26 DD75 pKa = 3.75 DD76 pKa = 5.02 EE77 pKa = 5.52 HH78 pKa = 8.85 LVDD81 pKa = 4.02 LPKK84 pKa = 11.0 NIFEE88 pKa = 4.37 VDD90 pKa = 3.53 GKK92 pKa = 10.87 VDD94 pKa = 3.45 EE95 pKa = 4.63 SVVFEE100 pKa = 4.66 GNTIPMMDD108 pKa = 3.38 SNGNRR113 pKa = 11.84 LNGSVVSVSDD123 pKa = 3.95 DD124 pKa = 3.39 TVQMDD129 pKa = 4.73 FNHH132 pKa = 6.85 PLAGEE137 pKa = 4.2 TLHH140 pKa = 6.11 FTGNVLSIRR149 pKa = 11.84 EE150 pKa = 4.01 ASAEE154 pKa = 3.94 EE155 pKa = 3.93 IAALFAPQGGGCGSGCGCGSSDD177 pKa = 3.55 EE178 pKa = 4.89 SEE180 pKa = 4.62 GCGSGCGCGSSDD192 pKa = 3.38 EE193 pKa = 4.92 SEE195 pKa = 4.28 GSSCGSGCGCHH206 pKa = 6.4
Molecular weight: 21.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.884
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.732
Sillero 3.961
Patrickios 0.846
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A2V3PJX7|A0A2V3PJX7_9BACT Uncharacterized protein OS=Dysgonomonas alginatilytica OX=1605892 GN=CLV62_15220 PE=4 SV=1
MM1 pKa = 7.29 ATLTFYY7 pKa = 11.1 LRR9 pKa = 11.84 ASSRR13 pKa = 11.84 IGCHH17 pKa = 6.53 AGTLCFRR24 pKa = 11.84 IIHH27 pKa = 5.83 ARR29 pKa = 11.84 RR30 pKa = 11.84 VLSHH34 pKa = 5.47 STNIRR39 pKa = 11.84 LYY41 pKa = 10.03 CRR43 pKa = 11.84 EE44 pKa = 3.79 WDD46 pKa = 3.57 STSRR50 pKa = 11.84 RR51 pKa = 11.84 IVLSDD56 pKa = 3.18 SSEE59 pKa = 3.81 EE60 pKa = 4.06 RR61 pKa = 11.84 ITYY64 pKa = 9.87 LNKK67 pKa = 10.38 AEE69 pKa = 3.98 QQQQNIEE76 pKa = 3.8 QSFAHH81 pKa = 7.1 IIHH84 pKa = 6.31 NLEE87 pKa = 3.33 EE88 pKa = 4.11 SGRR91 pKa = 11.84 YY92 pKa = 8.58 RR93 pKa = 11.84 VEE95 pKa = 6.13 DD96 pKa = 2.85 IMGRR100 pKa = 11.84 YY101 pKa = 9.11 RR102 pKa = 11.84 LRR104 pKa = 11.84 LDD106 pKa = 3.51 SHH108 pKa = 6.21 WLYY111 pKa = 10.99 HH112 pKa = 5.1 FTGQQSHH119 pKa = 5.68 TLIASGRR126 pKa = 11.84 EE127 pKa = 3.7 RR128 pKa = 11.84 TARR131 pKa = 11.84 AYY133 pKa = 8.14 RR134 pKa = 11.84 TAARR138 pKa = 11.84 GLIAFNAGRR147 pKa = 11.84 DD148 pKa = 3.8 LPLKK152 pKa = 10.16 HH153 pKa = 6.73 LNSCLIKK160 pKa = 10.89 DD161 pKa = 4.49 FEE163 pKa = 5.23 NDD165 pKa = 3.24 MKK167 pKa = 11.27 HH168 pKa = 6.6 KK169 pKa = 10.69 GKK171 pKa = 8.13 TLNTISFYY179 pKa = 10.26 MRR181 pKa = 11.84 NLRR184 pKa = 11.84 AIYY187 pKa = 10.18 NKK189 pKa = 10.44 AIACKK194 pKa = 10.09 RR195 pKa = 11.84 IADD198 pKa = 3.78 TGQNPFSDD206 pKa = 4.32 VYY208 pKa = 10.99 TGVQKK213 pKa = 9.54 TRR215 pKa = 11.84 KK216 pKa = 9.28 RR217 pKa = 11.84 SLTMDD222 pKa = 3.26 QLKK225 pKa = 8.51 TLNDD229 pKa = 3.86 LDD231 pKa = 4.67 LSLGSGTTAGAKK243 pKa = 9.53 EE244 pKa = 4.17 GSSVRR249 pKa = 11.84 DD250 pKa = 3.28 QEE252 pKa = 4.13 IFRR255 pKa = 11.84 GLDD258 pKa = 2.85 AARR261 pKa = 11.84 RR262 pKa = 11.84 LFLFCFHH269 pKa = 6.83 ARR271 pKa = 11.84 GMSFVDD277 pKa = 3.74 LAYY280 pKa = 10.55 LRR282 pKa = 11.84 KK283 pKa = 10.12 EE284 pKa = 4.04 NIQGKK289 pKa = 9.05 RR290 pKa = 11.84 IRR292 pKa = 11.84 YY293 pKa = 7.09 YY294 pKa = 10.19 RR295 pKa = 11.84 KK296 pKa = 8.28 KK297 pKa = 8.92 TGGLIEE303 pKa = 5.01 LKK305 pKa = 8.82 ITPVMQEE312 pKa = 4.19 IIHH315 pKa = 5.88 SFSLQTRR322 pKa = 11.84 DD323 pKa = 2.97 SLYY326 pKa = 10.04 IFPILVSPHH335 pKa = 6.02 KK336 pKa = 9.81 PLRR339 pKa = 11.84 LQYY342 pKa = 10.73 EE343 pKa = 4.19 SGLRR347 pKa = 11.84 LQNNRR352 pKa = 11.84 LKK354 pKa = 10.78 RR355 pKa = 11.84 LARR358 pKa = 11.84 LAGIHH363 pKa = 5.97 SAVSTHH369 pKa = 5.53 VSRR372 pKa = 11.84 HH373 pKa = 4.32 SWASAAKK380 pKa = 10.07 KK381 pKa = 10.14 EE382 pKa = 4.13 NLPVIPVRR390 pKa = 11.84 SMPRR394 pKa = 11.84 RR395 pKa = 3.52
Molecular weight: 45.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.692
IPC_protein 10.438
Toseland 10.511
ProMoST 10.277
Dawson 10.657
Bjellqvist 10.365
Wikipedia 10.862
Rodwell 10.891
Grimsley 10.716
Solomon 10.73
Lehninger 10.687
Nozaki 10.511
DTASelect 10.365
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.584
Patrickios 10.54
IPC_peptide 10.73
IPC2_peptide 9.37
IPC2.peptide.svr19 8.593
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4322
0
4322
1489738
29
2897
344.7
38.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.255 ± 0.036
0.955 ± 0.012
5.763 ± 0.023
6.211 ± 0.031
4.816 ± 0.029
6.421 ± 0.035
1.627 ± 0.015
8.071 ± 0.036
7.416 ± 0.03
9.055 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.347 ± 0.016
5.972 ± 0.033
3.48 ± 0.02
3.38 ± 0.022
3.86 ± 0.024
6.813 ± 0.029
5.736 ± 0.028
6.007 ± 0.029
1.219 ± 0.015
4.596 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here