Runella sp. SP2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Runella; unclassified Runella

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3GJR2|A0A3G3GJR2_9BACT FecR family protein OS=Runella sp. SP2 OX=2268026 GN=DTQ70_05275 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.37ALQTFIFVLSLFCIVKK18 pKa = 10.61GNAQDD23 pKa = 3.41VGINILNQPASVSQGSTNGRR43 pKa = 11.84IIIDD47 pKa = 3.17ICNFDD52 pKa = 4.22GGTTSTPLNRR62 pKa = 11.84IQPLITLPSALVDD75 pKa = 3.57TFVTAISFDD84 pKa = 3.05GWSIVSKK91 pKa = 10.53SGSTIRR97 pKa = 11.84FQNTVSITPGEE108 pKa = 4.31CKK110 pKa = 10.4QIVLGYY116 pKa = 10.25KK117 pKa = 9.51GVNVGGPLTVTGTIQFSGPQTSGNNPANDD146 pKa = 3.38NSTTSIAVTSAPVDD160 pKa = 3.49TDD162 pKa = 3.23GDD164 pKa = 4.53GIPDD168 pKa = 3.83STDD171 pKa = 3.7LDD173 pKa = 4.3DD174 pKa = 6.68DD175 pKa = 4.23NDD177 pKa = 5.09GILDD181 pKa = 3.91TVEE184 pKa = 3.91NAAVCSTVLFNGVANTDD201 pKa = 3.69CDD203 pKa = 4.01GDD205 pKa = 4.4GIPNYY210 pKa = 10.62LDD212 pKa = 4.76LDD214 pKa = 3.98SDD216 pKa = 3.89NDD218 pKa = 4.58GINDD222 pKa = 3.36VNEE225 pKa = 4.07AAGVDD230 pKa = 3.81VNGDD234 pKa = 3.47GFADD238 pKa = 4.28GTPDD242 pKa = 5.05AITGIPASAGNGLTPPNTDD261 pKa = 2.64GTGGTDD267 pKa = 4.17PYY269 pKa = 11.65DD270 pKa = 4.27LDD272 pKa = 4.39SDD274 pKa = 4.33NDD276 pKa = 4.14GQSDD280 pKa = 3.87LAEE283 pKa = 4.9GGLNASILDD292 pKa = 3.89PDD294 pKa = 3.91GDD296 pKa = 4.31GIVNCTANCDD306 pKa = 3.8PDD308 pKa = 4.02KK309 pKa = 11.39DD310 pKa = 4.52GILAPVDD317 pKa = 4.05GLPAAYY323 pKa = 10.1GDD325 pKa = 5.05AITNRR330 pKa = 11.84PPVANNDD337 pKa = 3.66TYY339 pKa = 11.58SILQGVILTDD349 pKa = 3.42NVLTNDD355 pKa = 3.9TDD357 pKa = 4.14PDD359 pKa = 4.14NNTLTVATTPTVAPTQGTVVLQTNGAFTYY388 pKa = 9.35TPTSATFTGQDD399 pKa = 3.35SFCYY403 pKa = 9.71LVCDD407 pKa = 3.52NGTPSLCKK415 pKa = 9.18TACATINIGAGIVDD429 pKa = 4.05LSISKK434 pKa = 10.29VLVGSKK440 pKa = 9.65IRR442 pKa = 11.84SLNDD446 pKa = 3.28TITYY450 pKa = 9.9RR451 pKa = 11.84IVVKK455 pKa = 9.79NTGNITATNIVVEE468 pKa = 4.68DD469 pKa = 3.73STTTGLQIISGTPTSGSFSGTNWTIPSLASGDD501 pKa = 3.75STTLVIQAKK510 pKa = 10.07VIAEE514 pKa = 3.79GVNFNFALIKK524 pKa = 10.85SADD527 pKa = 3.74QPDD530 pKa = 4.13TVATNDD536 pKa = 3.66TANDD540 pKa = 4.68CITVPIGLCSGQKK553 pKa = 10.0VEE555 pKa = 4.2VSVPSTYY562 pKa = 11.12TNVIWFKK569 pKa = 11.04NGAQIASGNVVLFSEE584 pKa = 4.54EE585 pKa = 3.53GTYY588 pKa = 10.54TFTASNVTCPVSGCCPLIIQSGTNCCPVQLCIPVTVVRR626 pKa = 11.84RR627 pKa = 11.84KK628 pKa = 9.75KK629 pKa = 10.53

Molecular weight: 64.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3GRY5|A0A3G3GRY5_9BACT Uncharacterized protein OS=Runella sp. SP2 OX=2268026 GN=DTQ70_18960 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.3KK3 pKa = 9.77IVEE6 pKa = 4.15FLQLGEE12 pKa = 4.04VFGYY16 pKa = 8.46FLRR19 pKa = 11.84VFQKK23 pKa = 10.17PDD25 pKa = 3.38PSRR28 pKa = 11.84PSTTSLRR35 pKa = 11.84VMHH38 pKa = 7.16GINRR42 pKa = 11.84ISIVMFLFCLGVMLYY57 pKa = 10.62RR58 pKa = 11.84FFTRR62 pKa = 4.43

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5998

0

5998

2298142

25

4859

383.2

42.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.595 ± 0.033

0.9 ± 0.017

4.91 ± 0.025

5.628 ± 0.041

4.896 ± 0.027

7.102 ± 0.047

1.76 ± 0.02

6.175 ± 0.028

6.226 ± 0.043

9.506 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.02

5.307 ± 0.04

4.177 ± 0.02

4.103 ± 0.023

4.267 ± 0.03

6.562 ± 0.043

6.627 ± 0.069

6.886 ± 0.034

1.379 ± 0.013

3.899 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski