Amino acid dipepetide frequency for Staphylococcus phage PT1028

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.217AlaAla: 1.217 ± 0.808
0.73AlaCys: 0.73 ± 0.321
1.46AlaAsp: 1.46 ± 0.516
1.46AlaGlu: 1.46 ± 0.501
1.946AlaPhe: 1.946 ± 0.816
2.92AlaGly: 2.92 ± 0.999
0.73AlaHis: 0.73 ± 0.402
4.136AlaIle: 4.136 ± 0.954
3.163AlaLys: 3.163 ± 0.811
3.893AlaLeu: 3.893 ± 0.943
0.487AlaMet: 0.487 ± 0.313
2.676AlaAsn: 2.676 ± 0.743
0.243AlaPro: 0.243 ± 0.218
1.946AlaGln: 1.946 ± 0.767
2.433AlaArg: 2.433 ± 0.815
5.109AlaSer: 5.109 ± 1.24
2.433AlaThr: 2.433 ± 0.668
1.217AlaVal: 1.217 ± 0.395
0.487AlaTrp: 0.487 ± 0.3
0.973AlaTyr: 0.973 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
0.243CysAla: 0.243 ± 0.281
0.243CysCys: 0.243 ± 0.268
0.73CysAsp: 0.73 ± 0.432
0.487CysGlu: 0.487 ± 0.251
1.46CysPhe: 1.46 ± 0.485
0.487CysGly: 0.487 ± 0.334
0.243CysHis: 0.243 ± 0.218
0.487CysIle: 0.487 ± 0.258
0.487CysLys: 0.487 ± 0.279
0.243CysLeu: 0.243 ± 0.22
0.0CysMet: 0.0 ± 0.0
0.243CysAsn: 0.243 ± 0.242
0.243CysPro: 0.243 ± 0.218
0.243CysGln: 0.243 ± 0.191
0.243CysArg: 0.243 ± 0.254
0.973CysSer: 0.973 ± 0.507
0.0CysThr: 0.0 ± 0.0
0.243CysVal: 0.243 ± 0.269
0.0CysTrp: 0.0 ± 0.0
0.73CysTyr: 0.73 ± 0.361
0.0CysXaa: 0.0 ± 0.0
Asp
1.703AspAla: 1.703 ± 0.573
0.73AspCys: 0.73 ± 0.351
3.65AspAsp: 3.65 ± 1.039
5.596AspGlu: 5.596 ± 0.968
1.46AspPhe: 1.46 ± 0.472
3.65AspGly: 3.65 ± 0.819
0.973AspHis: 0.973 ± 0.411
4.623AspIle: 4.623 ± 1.119
6.569AspLys: 6.569 ± 1.113
4.866AspLeu: 4.866 ± 1.23
2.433AspMet: 2.433 ± 0.818
3.893AspAsn: 3.893 ± 0.755
1.217AspPro: 1.217 ± 0.359
0.73AspGln: 0.73 ± 0.38
2.19AspArg: 2.19 ± 0.669
3.406AspSer: 3.406 ± 0.957
1.946AspThr: 1.946 ± 0.645
5.109AspVal: 5.109 ± 0.922
0.243AspTrp: 0.243 ± 0.242
3.893AspTyr: 3.893 ± 0.972
0.0AspXaa: 0.0 ± 0.0
Glu
1.703GluAla: 1.703 ± 0.553
0.487GluCys: 0.487 ± 0.349
3.65GluAsp: 3.65 ± 0.883
4.38GluGlu: 4.38 ± 1.256
3.406GluPhe: 3.406 ± 0.785
2.92GluGly: 2.92 ± 1.061
2.433GluHis: 2.433 ± 0.714
6.569GluIle: 6.569 ± 1.237
6.813GluLys: 6.813 ± 1.066
8.029GluLeu: 8.029 ± 1.647
2.92GluMet: 2.92 ± 0.667
5.596GluAsn: 5.596 ± 1.224
1.46GluPro: 1.46 ± 0.603
4.38GluGln: 4.38 ± 1.102
3.65GluArg: 3.65 ± 0.832
4.136GluSer: 4.136 ± 1.258
4.136GluThr: 4.136 ± 1.231
3.893GluVal: 3.893 ± 1.212
1.46GluTrp: 1.46 ± 0.536
6.083GluTyr: 6.083 ± 1.254
0.0GluXaa: 0.0 ± 0.0
Phe
2.676PheAla: 2.676 ± 0.818
0.0PheCys: 0.0 ± 0.0
3.406PheAsp: 3.406 ± 0.787
4.136PheGlu: 4.136 ± 1.15
0.973PhePhe: 0.973 ± 0.503
2.92PheGly: 2.92 ± 0.493
0.73PheHis: 0.73 ± 0.511
2.433PheIle: 2.433 ± 0.664
4.136PheLys: 4.136 ± 0.885
1.46PheLeu: 1.46 ± 0.7
0.73PheMet: 0.73 ± 0.443
3.406PheAsn: 3.406 ± 0.85
0.73PhePro: 0.73 ± 0.309
1.46PheGln: 1.46 ± 0.547
0.73PheArg: 0.73 ± 0.397
2.676PheSer: 2.676 ± 0.776
2.433PheThr: 2.433 ± 0.601
1.46PheVal: 1.46 ± 0.4
0.487PheTrp: 0.487 ± 0.326
0.73PheTyr: 0.73 ± 0.323
0.0PheXaa: 0.0 ± 0.0
Gly
1.946GlyAla: 1.946 ± 1.225
0.73GlyCys: 0.73 ± 0.383
3.163GlyAsp: 3.163 ± 0.862
3.406GlyGlu: 3.406 ± 0.93
2.433GlyPhe: 2.433 ± 0.717
2.19GlyGly: 2.19 ± 1.363
2.433GlyHis: 2.433 ± 0.732
4.136GlyIle: 4.136 ± 1.063
4.866GlyLys: 4.866 ± 1.224
3.893GlyLeu: 3.893 ± 1.201
0.487GlyMet: 0.487 ± 0.286
1.703GlyAsn: 1.703 ± 0.702
0.0GlyPro: 0.0 ± 0.0
2.19GlyGln: 2.19 ± 0.722
1.46GlyArg: 1.46 ± 0.588
3.65GlySer: 3.65 ± 0.687
2.92GlyThr: 2.92 ± 1.068
2.19GlyVal: 2.19 ± 0.775
0.487GlyTrp: 0.487 ± 0.346
3.893GlyTyr: 3.893 ± 0.96
0.0GlyXaa: 0.0 ± 0.0
His
0.487HisAla: 0.487 ± 0.292
0.0HisCys: 0.0 ± 0.0
2.19HisAsp: 2.19 ± 0.554
2.19HisGlu: 2.19 ± 0.862
0.973HisPhe: 0.973 ± 0.376
0.487HisGly: 0.487 ± 0.365
0.243HisHis: 0.243 ± 0.195
1.46HisIle: 1.46 ± 0.529
1.946HisLys: 1.946 ± 0.661
1.946HisLeu: 1.946 ± 0.839
0.0HisMet: 0.0 ± 0.0
1.217HisAsn: 1.217 ± 0.545
0.487HisPro: 0.487 ± 0.258
0.73HisGln: 0.73 ± 0.372
0.973HisArg: 0.973 ± 0.527
1.703HisSer: 1.703 ± 0.735
1.703HisThr: 1.703 ± 0.705
3.163HisVal: 3.163 ± 0.925
0.0HisTrp: 0.0 ± 0.0
1.217HisTyr: 1.217 ± 0.48
0.0HisXaa: 0.0 ± 0.0
Ile
3.163IleAla: 3.163 ± 1.11
0.243IleCys: 0.243 ± 0.213
5.109IleAsp: 5.109 ± 1.047
9.976IleGlu: 9.976 ± 1.366
3.163IlePhe: 3.163 ± 1.05
4.38IleGly: 4.38 ± 0.983
0.487IleHis: 0.487 ± 0.365
4.136IleIle: 4.136 ± 0.607
10.219IleLys: 10.219 ± 1.113
3.163IleLeu: 3.163 ± 0.712
1.217IleMet: 1.217 ± 0.455
6.326IleAsn: 6.326 ± 1.114
3.163IlePro: 3.163 ± 0.595
2.19IleGln: 2.19 ± 0.674
2.19IleArg: 2.19 ± 0.546
6.813IleSer: 6.813 ± 1.345
4.38IleThr: 4.38 ± 0.762
3.893IleVal: 3.893 ± 0.856
0.0IleTrp: 0.0 ± 0.0
3.163IleTyr: 3.163 ± 0.672
0.0IleXaa: 0.0 ± 0.0
Lys
4.136LysAla: 4.136 ± 1.016
0.243LysCys: 0.243 ± 0.258
6.569LysAsp: 6.569 ± 1.2
9.976LysGlu: 9.976 ± 1.725
1.46LysPhe: 1.46 ± 0.424
4.38LysGly: 4.38 ± 1.423
2.676LysHis: 2.676 ± 0.816
6.569LysIle: 6.569 ± 1.068
8.273LysLys: 8.273 ± 1.416
9.489LysLeu: 9.489 ± 1.29
1.946LysMet: 1.946 ± 0.482
7.056LysAsn: 7.056 ± 0.962
3.163LysPro: 3.163 ± 0.698
5.353LysGln: 5.353 ± 0.926
4.623LysArg: 4.623 ± 0.935
4.866LysSer: 4.866 ± 1.151
6.326LysThr: 6.326 ± 1.342
6.083LysVal: 6.083 ± 1.114
0.973LysTrp: 0.973 ± 0.451
4.38LysTyr: 4.38 ± 0.938
0.0LysXaa: 0.0 ± 0.0
Leu
3.163LeuAla: 3.163 ± 0.557
0.73LeuCys: 0.73 ± 0.379
4.38LeuAsp: 4.38 ± 0.89
9.489LeuGlu: 9.489 ± 1.868
3.406LeuPhe: 3.406 ± 0.987
4.38LeuGly: 4.38 ± 1.529
1.46LeuHis: 1.46 ± 0.426
7.056LeuIle: 7.056 ± 1.155
8.029LeuLys: 8.029 ± 1.272
8.516LeuLeu: 8.516 ± 1.145
0.487LeuMet: 0.487 ± 0.271
7.056LeuAsn: 7.056 ± 1.042
1.703LeuPro: 1.703 ± 0.579
4.38LeuGln: 4.38 ± 1.087
2.676LeuArg: 2.676 ± 0.662
5.839LeuSer: 5.839 ± 1.332
3.406LeuThr: 3.406 ± 0.922
5.353LeuVal: 5.353 ± 0.705
0.487LeuTrp: 0.487 ± 0.326
3.65LeuTyr: 3.65 ± 0.898
0.0LeuXaa: 0.0 ± 0.0
Met
1.46MetAla: 1.46 ± 0.462
0.487MetCys: 0.487 ± 0.297
0.973MetAsp: 0.973 ± 0.47
0.487MetGlu: 0.487 ± 0.298
1.217MetPhe: 1.217 ± 0.447
0.973MetGly: 0.973 ± 0.345
0.0MetHis: 0.0 ± 0.0
0.973MetIle: 0.973 ± 0.494
2.433MetLys: 2.433 ± 0.709
2.676MetLeu: 2.676 ± 0.646
0.487MetMet: 0.487 ± 0.297
2.433MetAsn: 2.433 ± 0.854
0.487MetPro: 0.487 ± 0.341
0.973MetGln: 0.973 ± 0.57
1.217MetArg: 1.217 ± 0.491
0.487MetSer: 0.487 ± 0.319
0.973MetThr: 0.973 ± 0.458
1.703MetVal: 1.703 ± 0.636
0.243MetTrp: 0.243 ± 0.213
0.73MetTyr: 0.73 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
5.109AsnAla: 5.109 ± 0.855
0.243AsnCys: 0.243 ± 0.22
4.623AsnAsp: 4.623 ± 1.003
5.596AsnGlu: 5.596 ± 0.998
1.46AsnPhe: 1.46 ± 0.642
3.893AsnGly: 3.893 ± 0.823
1.46AsnHis: 1.46 ± 0.675
7.299AsnIle: 7.299 ± 0.918
9.002AsnLys: 9.002 ± 1.556
3.893AsnLeu: 3.893 ± 1.04
1.46AsnMet: 1.46 ± 0.487
6.083AsnAsn: 6.083 ± 1.138
2.19AsnPro: 2.19 ± 0.823
2.19AsnGln: 2.19 ± 0.687
1.217AsnArg: 1.217 ± 0.426
5.353AsnSer: 5.353 ± 1.016
2.676AsnThr: 2.676 ± 0.649
5.353AsnVal: 5.353 ± 1.261
0.973AsnTrp: 0.973 ± 0.373
2.92AsnTyr: 2.92 ± 0.776
0.0AsnXaa: 0.0 ± 0.0
Pro
0.73ProAla: 0.73 ± 0.434
0.243ProCys: 0.243 ± 0.268
0.73ProAsp: 0.73 ± 0.457
0.487ProGlu: 0.487 ± 0.292
0.73ProPhe: 0.73 ± 0.285
0.243ProGly: 0.243 ± 0.258
0.487ProHis: 0.487 ± 0.335
1.946ProIle: 1.946 ± 0.694
2.19ProLys: 2.19 ± 0.671
2.676ProLeu: 2.676 ± 0.659
0.973ProMet: 0.973 ± 0.483
2.19ProAsn: 2.19 ± 0.779
0.73ProPro: 0.73 ± 0.434
0.73ProGln: 0.73 ± 0.393
0.487ProArg: 0.487 ± 0.454
2.19ProSer: 2.19 ± 0.605
0.973ProThr: 0.973 ± 0.471
0.973ProVal: 0.973 ± 0.42
0.0ProTrp: 0.0 ± 0.0
3.163ProTyr: 3.163 ± 0.662
0.0ProXaa: 0.0 ± 0.0
Gln
2.19GlnAla: 2.19 ± 0.666
0.487GlnCys: 0.487 ± 0.258
1.46GlnAsp: 1.46 ± 0.456
1.946GlnGlu: 1.946 ± 0.672
1.703GlnPhe: 1.703 ± 0.495
1.217GlnGly: 1.217 ± 0.522
0.0GlnHis: 0.0 ± 0.0
2.19GlnIle: 2.19 ± 0.511
3.893GlnLys: 3.893 ± 0.793
4.623GlnLeu: 4.623 ± 1.073
0.973GlnMet: 0.973 ± 0.418
1.703GlnAsn: 1.703 ± 0.532
1.46GlnPro: 1.46 ± 0.597
1.46GlnGln: 1.46 ± 0.608
2.433GlnArg: 2.433 ± 0.763
4.38GlnSer: 4.38 ± 0.824
2.19GlnThr: 2.19 ± 0.513
2.433GlnVal: 2.433 ± 0.754
0.73GlnTrp: 0.73 ± 0.288
0.973GlnTyr: 0.973 ± 0.47
0.0GlnXaa: 0.0 ± 0.0
Arg
1.217ArgAla: 1.217 ± 0.455
0.487ArgCys: 0.487 ± 0.436
2.19ArgAsp: 2.19 ± 0.688
2.19ArgGlu: 2.19 ± 0.626
1.217ArgPhe: 1.217 ± 0.456
1.946ArgGly: 1.946 ± 0.406
0.973ArgHis: 0.973 ± 0.519
2.676ArgIle: 2.676 ± 0.7
1.46ArgLys: 1.46 ± 0.442
3.893ArgLeu: 3.893 ± 0.903
0.243ArgMet: 0.243 ± 0.239
3.893ArgAsn: 3.893 ± 1.038
0.0ArgPro: 0.0 ± 0.0
1.703ArgGln: 1.703 ± 0.771
1.46ArgArg: 1.46 ± 0.479
3.893ArgSer: 3.893 ± 1.023
2.19ArgThr: 2.19 ± 0.663
3.163ArgVal: 3.163 ± 0.837
0.243ArgTrp: 0.243 ± 0.248
2.676ArgTyr: 2.676 ± 0.666
0.0ArgXaa: 0.0 ± 0.0
Ser
3.163SerAla: 3.163 ± 0.98
0.487SerCys: 0.487 ± 0.37
4.623SerAsp: 4.623 ± 0.92
4.136SerGlu: 4.136 ± 0.958
4.136SerPhe: 4.136 ± 1.078
2.92SerGly: 2.92 ± 0.627
1.703SerHis: 1.703 ± 0.432
5.596SerIle: 5.596 ± 0.903
8.516SerLys: 8.516 ± 1.263
4.623SerLeu: 4.623 ± 0.813
1.703SerMet: 1.703 ± 0.508
6.326SerAsn: 6.326 ± 1.12
1.46SerPro: 1.46 ± 0.657
1.46SerGln: 1.46 ± 0.479
1.946SerArg: 1.946 ± 0.675
6.326SerSer: 6.326 ± 1.158
4.623SerThr: 4.623 ± 1.364
4.866SerVal: 4.866 ± 0.835
0.487SerTrp: 0.487 ± 0.334
4.623SerTyr: 4.623 ± 0.99
0.0SerXaa: 0.0 ± 0.0
Thr
1.946ThrAla: 1.946 ± 0.754
0.243ThrCys: 0.243 ± 0.22
3.406ThrAsp: 3.406 ± 1.39
4.866ThrGlu: 4.866 ± 0.988
1.46ThrPhe: 1.46 ± 0.377
4.136ThrGly: 4.136 ± 1.056
2.433ThrHis: 2.433 ± 1.047
3.893ThrIle: 3.893 ± 0.965
4.136ThrLys: 4.136 ± 0.866
5.839ThrLeu: 5.839 ± 1.011
0.487ThrMet: 0.487 ± 0.364
2.676ThrAsn: 2.676 ± 0.803
0.973ThrPro: 0.973 ± 0.438
1.946ThrGln: 1.946 ± 0.526
2.676ThrArg: 2.676 ± 0.635
3.406ThrSer: 3.406 ± 1.237
4.38ThrThr: 4.38 ± 1.323
4.136ThrVal: 4.136 ± 1.202
0.73ThrTrp: 0.73 ± 0.416
1.46ThrTyr: 1.46 ± 0.358
0.0ThrXaa: 0.0 ± 0.0
Val
1.946ValAla: 1.946 ± 0.613
0.243ValCys: 0.243 ± 0.253
3.406ValAsp: 3.406 ± 0.82
2.676ValGlu: 2.676 ± 1.019
2.676ValPhe: 2.676 ± 0.523
1.703ValGly: 1.703 ± 0.526
0.73ValHis: 0.73 ± 0.352
6.083ValIle: 6.083 ± 1.065
5.596ValLys: 5.596 ± 1.151
6.813ValLeu: 6.813 ± 1.255
2.92ValMet: 2.92 ± 1.165
3.893ValAsn: 3.893 ± 0.834
1.46ValPro: 1.46 ± 0.839
1.703ValGln: 1.703 ± 0.6
1.946ValArg: 1.946 ± 0.773
4.38ValSer: 4.38 ± 1.047
5.353ValThr: 5.353 ± 0.923
3.406ValVal: 3.406 ± 1.254
0.487ValTrp: 0.487 ± 0.258
3.406ValTyr: 3.406 ± 0.876
0.0ValXaa: 0.0 ± 0.0
Trp
0.243TrpAla: 0.243 ± 0.213
0.487TrpCys: 0.487 ± 0.343
0.73TrpAsp: 0.73 ± 0.45
0.487TrpGlu: 0.487 ± 0.333
0.243TrpPhe: 0.243 ± 0.218
0.73TrpGly: 0.73 ± 0.434
0.73TrpHis: 0.73 ± 0.356
0.243TrpIle: 0.243 ± 0.268
0.243TrpLys: 0.243 ± 0.223
0.973TrpLeu: 0.973 ± 0.4
0.487TrpMet: 0.487 ± 0.341
0.487TrpAsn: 0.487 ± 0.283
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.73TrpArg: 0.73 ± 0.4
0.973TrpSer: 0.973 ± 0.532
0.243TrpThr: 0.243 ± 0.241
0.973TrpVal: 0.973 ± 0.468
0.0TrpTrp: 0.0 ± 0.0
0.243TrpTyr: 0.243 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.217TyrAla: 1.217 ± 0.338
0.487TyrCys: 0.487 ± 0.321
2.433TyrAsp: 2.433 ± 0.62
4.136TyrGlu: 4.136 ± 0.826
2.19TyrPhe: 2.19 ± 0.596
1.703TyrGly: 1.703 ± 0.54
2.19TyrHis: 2.19 ± 0.805
4.866TyrIle: 4.866 ± 1.11
6.569TyrLys: 6.569 ± 0.95
4.136TyrLeu: 4.136 ± 0.979
0.73TyrMet: 0.73 ± 0.348
4.136TyrAsn: 4.136 ± 0.96
1.703TyrPro: 1.703 ± 0.507
2.676TyrGln: 2.676 ± 0.582
2.433TyrArg: 2.433 ± 0.636
3.163TyrSer: 3.163 ± 0.81
1.946TyrThr: 1.946 ± 0.621
1.46TyrVal: 1.46 ± 0.662
0.73TyrTrp: 0.73 ± 0.378
3.406TyrTyr: 3.406 ± 0.955
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 22 proteins (4111 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski