Giardia intestinalis (strain P15) (Giardia lamblia)

Taxonomy: cellular organisms; Eukaryota; Metamonada; Fornicata; Diplomonadida; Hexamitidae; Giardiinae; Giardia; Giardia intestinalis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4998 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1F975|E1F975_GIAIA Protein 21.1 OS=Giardia intestinalis (strain P15) OX=658858 GN=GLP15_3195 PE=4 SV=1
MM1 pKa = 7.66TLRR4 pKa = 11.84CIYY7 pKa = 10.35FPDD10 pKa = 4.44GEE12 pKa = 4.37NPIPSRR18 pKa = 11.84IIIEE22 pKa = 4.25PPLKK26 pKa = 10.41KK27 pKa = 10.18RR28 pKa = 11.84GGLPTTFQCSLQEE41 pKa = 4.04QTGSDD46 pKa = 4.17LIISGSCITMDD57 pKa = 4.91GDD59 pKa = 3.87TLLINARR66 pKa = 11.84EE67 pKa = 4.2DD68 pKa = 3.83TTPVAYY74 pKa = 8.94TVEE77 pKa = 4.63HH78 pKa = 6.58IGWLTQDD85 pKa = 3.91AYY87 pKa = 10.67EE88 pKa = 4.59EE89 pKa = 4.22LQQTADD95 pKa = 3.3YY96 pKa = 9.6WDD98 pKa = 3.42QLAEE102 pKa = 4.08YY103 pKa = 10.26SSDD106 pKa = 3.41TFDD109 pKa = 5.35PEE111 pKa = 4.82DD112 pKa = 4.26SNCEE116 pKa = 3.69GARR119 pKa = 11.84GNDD122 pKa = 3.66YY123 pKa = 10.81PSTPTSRR130 pKa = 11.84EE131 pKa = 3.68SDD133 pKa = 3.73VEE135 pKa = 3.97EE136 pKa = 4.04WDD138 pKa = 3.39

Molecular weight: 15.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1F6A2|E1F6A2_GIAIA ARID1 protein OS=Giardia intestinalis (strain P15) OX=658858 GN=GLP15_4122 PE=4 SV=1
MM1 pKa = 7.34NPTVKK6 pKa = 10.25VFGPTGTQVAEE17 pKa = 4.29LPRR20 pKa = 11.84PRR22 pKa = 11.84VFNVPLRR29 pKa = 11.84PDD31 pKa = 3.99IINFVHH37 pKa = 5.51TQLRR41 pKa = 11.84KK42 pKa = 9.71CLRR45 pKa = 11.84TPYY48 pKa = 10.4AVSRR52 pKa = 11.84YY53 pKa = 10.48AGVQCTAHH61 pKa = 5.25SWGPGRR67 pKa = 11.84AVARR71 pKa = 11.84LPRR74 pKa = 11.84KK75 pKa = 9.66HH76 pKa = 6.43GGIGAYY82 pKa = 10.49ANFARR87 pKa = 11.84GGHH90 pKa = 5.4MAHH93 pKa = 6.95PTTVIRR99 pKa = 11.84RR100 pKa = 11.84WCRR103 pKa = 11.84KK104 pKa = 8.57VNLNQRR110 pKa = 11.84RR111 pKa = 11.84YY112 pKa = 9.75AVASALAASANASLVEE128 pKa = 4.1ARR130 pKa = 11.84GHH132 pKa = 6.83RR133 pKa = 11.84IADD136 pKa = 3.57VPSIPLVVDD145 pKa = 3.55TDD147 pKa = 3.76NVTKK151 pKa = 10.51TKK153 pKa = 10.67DD154 pKa = 2.91ALAIIKK160 pKa = 10.23AVGVLRR166 pKa = 11.84DD167 pKa = 3.44VEE169 pKa = 4.19RR170 pKa = 11.84CKK172 pKa = 10.79DD173 pKa = 3.44SRR175 pKa = 11.84HH176 pKa = 4.91IRR178 pKa = 11.84AGRR181 pKa = 11.84GKK183 pKa = 9.03MRR185 pKa = 11.84NRR187 pKa = 11.84RR188 pKa = 11.84YY189 pKa = 8.63ITRR192 pKa = 11.84KK193 pKa = 9.51GPLVIYY199 pKa = 8.86NTEE202 pKa = 3.73DD203 pKa = 3.06VARR206 pKa = 11.84GFRR209 pKa = 11.84NISGVDD215 pKa = 3.49LCHH218 pKa = 6.43VSSIRR223 pKa = 11.84LLEE226 pKa = 4.15LAPGSHH232 pKa = 7.85PGRR235 pKa = 11.84LILWTKK241 pKa = 10.44SAFASLDD248 pKa = 3.27DD249 pKa = 4.71VYY251 pKa = 11.33AAKK254 pKa = 10.36QNYY257 pKa = 6.77TLPRR261 pKa = 11.84ACITQTDD268 pKa = 3.89IEE270 pKa = 5.22RR271 pKa = 11.84ILQSDD276 pKa = 4.47AVQKK280 pKa = 9.65TFVPKK285 pKa = 10.03RR286 pKa = 11.84DD287 pKa = 3.51PLVIEE292 pKa = 4.52RR293 pKa = 11.84KK294 pKa = 9.63VDD296 pKa = 3.49PFSSKK301 pKa = 10.23EE302 pKa = 3.57ALARR306 pKa = 11.84LDD308 pKa = 3.37PSGTVMSKK316 pKa = 10.75

Molecular weight: 35.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4998

0

4998

3057358

38

8149

611.7

68.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.034

2.433 ± 0.043

5.393 ± 0.017

5.879 ± 0.031

3.538 ± 0.023

5.218 ± 0.04

2.401 ± 0.015

6.069 ± 0.023

5.217 ± 0.02

10.531 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.011

4.255 ± 0.016

4.525 ± 0.024

3.984 ± 0.02

5.19 ± 0.023

9.112 ± 0.036

6.543 ± 0.02

5.85 ± 0.017

0.653 ± 0.007

3.431 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski