Odoribacter laneus CAG:561
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2812 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5VJA3|R5VJA3_9BACT SusC/RagA family TonB-linked outer membrane protein OS=Odoribacter laneus CAG:561 OX=1263089 GN=BN709_00202 PE=4 SV=1
MM1 pKa = 7.85 NYY3 pKa = 9.72 RR4 pKa = 11.84 FEE6 pKa = 4.82 ISLPNSSDD14 pKa = 3.42 FRR16 pKa = 11.84 CEE18 pKa = 3.34 IAISGDD24 pKa = 3.19 QTFQQLHH31 pKa = 6.34 DD32 pKa = 5.23 KK33 pKa = 10.33 IIEE36 pKa = 4.01 VLGYY40 pKa = 10.4 DD41 pKa = 3.92 SSQMASFYY49 pKa = 10.92 TLDD52 pKa = 3.26 KK53 pKa = 9.83 MGNRR57 pKa = 11.84 VKK59 pKa = 10.55 EE60 pKa = 3.86 IALMEE65 pKa = 4.24 MSTDD69 pKa = 3.63 DD70 pKa = 4.1 EE71 pKa = 4.45 EE72 pKa = 6.76 ANTLVMDD79 pKa = 3.78 VTLIRR84 pKa = 11.84 EE85 pKa = 4.24 IVNASCIEE93 pKa = 3.92 LEE95 pKa = 4.28 YY96 pKa = 11.42 VFDD99 pKa = 4.57 FFNNRR104 pKa = 11.84 YY105 pKa = 9.06 FRR107 pKa = 11.84 VEE109 pKa = 3.71 YY110 pKa = 10.26 AGEE113 pKa = 4.17 YY114 pKa = 9.76 IADD117 pKa = 3.92 SADD120 pKa = 3.4 VLPVCLYY127 pKa = 10.7 CDD129 pKa = 3.66 GDD131 pKa = 3.42 IPEE134 pKa = 4.12 QMEE137 pKa = 4.92 IEE139 pKa = 5.42 DD140 pKa = 3.93 NDD142 pKa = 2.83 KK143 pKa = 9.97 WAYY146 pKa = 9.73 GKK148 pKa = 10.42 SDD150 pKa = 3.64 EE151 pKa = 4.94 EE152 pKa = 4.52 YY153 pKa = 10.27 DD154 pKa = 3.6 DD155 pKa = 5.18 SFMEE159 pKa = 4.21 EE160 pKa = 3.86 FSDD163 pKa = 3.99 FTEE166 pKa = 4.13 EE167 pKa = 4.29 GEE169 pKa = 4.28 NYY171 pKa = 10.39 GDD173 pKa = 4.0 EE174 pKa = 4.39 EE175 pKa = 4.27 EE176 pKa = 5.13 GGYY179 pKa = 10.66 DD180 pKa = 4.56 DD181 pKa = 6.18 EE182 pKa = 4.88 EE183 pKa = 5.74 DD184 pKa = 3.58 EE185 pKa = 4.73 FGGKK189 pKa = 9.1 GRR191 pKa = 11.84 YY192 pKa = 8.52 DD193 pKa = 3.53 DD194 pKa = 4.66 RR195 pKa = 11.84 YY196 pKa = 10.88 EE197 pKa = 5.39 SIDD200 pKa = 4.16 DD201 pKa = 4.25 YY202 pKa = 11.47 IDD204 pKa = 3.38 RR205 pKa = 11.84 LL206 pKa = 3.74
Molecular weight: 24.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.821
Patrickios 0.744
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|R5VI80|R5VI80_9BACT Uncharacterized protein OS=Odoribacter laneus CAG:561 OX=1263089 GN=BN709_02510 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 5.38 PAGTMTGANSYY13 pKa = 11.04 AEE15 pKa = 4.29 STSALTAFLAEE26 pKa = 4.17 MVFTFIFVLVVLGVTDD42 pKa = 3.71 SDD44 pKa = 3.31 KK45 pKa = 10.76 GTPVMCGLAIGLTLVLVHH63 pKa = 6.36 IVCIPITGTSVNPARR78 pKa = 11.84 SVGPALFEE86 pKa = 4.27 GGRR89 pKa = 11.84 ALSQLWLFIVAPSVGAALLAAGSSKK114 pKa = 10.85 LLYY117 pKa = 8.05 TTGKK121 pKa = 8.59 YY122 pKa = 10.17 CYY124 pKa = 9.93 LILICLSRR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 FIPVARR140 pKa = 11.84 SISKK144 pKa = 10.32 RR145 pKa = 11.84 VLCLICRR152 pKa = 11.84 RR153 pKa = 11.84 NRR155 pKa = 11.84 RR156 pKa = 11.84 ANGWRR161 pKa = 11.84 LSGGRR166 pKa = 11.84 RR167 pKa = 11.84 ITGWKK172 pKa = 10.12 LNN174 pKa = 3.79
Molecular weight: 18.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.589
IPC_protein 10.321
Toseland 10.379
ProMoST 10.131
Dawson 10.54
Bjellqvist 10.306
Wikipedia 10.76
Rodwell 10.73
Grimsley 10.613
Solomon 10.628
Lehninger 10.584
Nozaki 10.467
DTASelect 10.262
Thurlkill 10.423
EMBOSS 10.789
Sillero 10.482
Patrickios 10.452
IPC_peptide 10.628
IPC2_peptide 9.692
IPC2.peptide.svr19 8.419
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2812
0
2812
988689
29
2247
351.6
39.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.89 ± 0.044
1.258 ± 0.016
4.929 ± 0.029
7.018 ± 0.047
4.841 ± 0.036
6.84 ± 0.039
1.813 ± 0.017
7.532 ± 0.043
6.861 ± 0.043
9.686 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.541 ± 0.021
4.763 ± 0.036
3.787 ± 0.022
3.566 ± 0.021
4.712 ± 0.035
5.709 ± 0.029
5.278 ± 0.034
6.365 ± 0.038
1.215 ± 0.017
4.398 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here