Roseovarius pacificus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6WL23|A0A1M6WL23_9RHOB LuxR family transcriptional regulator OS=Roseovarius pacificus OX=337701 GN=SAMN05444398_10162 PE=4 SV=1
MM1 pKa = 7.6TIADD5 pKa = 4.36TMTLDD10 pKa = 3.57ALAQIAHH17 pKa = 5.05QTYY20 pKa = 6.96QQRR23 pKa = 11.84YY24 pKa = 8.17NPDD27 pKa = 2.69GSINTGSLNDD37 pKa = 4.04PVAGSNGSFYY47 pKa = 10.93TEE49 pKa = 3.28ITQIVNGPLGFQARR63 pKa = 11.84AFFNEE68 pKa = 4.09TTNEE72 pKa = 3.73LVIAFTGTEE81 pKa = 4.23GFPNDD86 pKa = 3.86PAEE89 pKa = 4.14FLPDD93 pKa = 3.95FLADD97 pKa = 4.33FSLAVAGVSPQDD109 pKa = 3.51LAAQAFIKK117 pKa = 10.52QATTLAQLEE126 pKa = 4.35AGPFGSFDD134 pKa = 3.35VTYY137 pKa = 10.39VGHH140 pKa = 6.31SLGGFLAQTASASGPEE156 pKa = 4.17GEE158 pKa = 4.17VVVFNAPGAGGFLGLPEE175 pKa = 4.13NHH177 pKa = 7.32PFPEE181 pKa = 4.55DD182 pKa = 3.09NFTYY186 pKa = 10.44VYY188 pKa = 10.67SDD190 pKa = 3.55PSDD193 pKa = 3.06WGAMGGAIHH202 pKa = 6.73SAGRR206 pKa = 11.84PLSDD210 pKa = 3.57NIYY213 pKa = 9.0YY214 pKa = 10.3VPGAEE219 pKa = 4.17GHH221 pKa = 7.15DD222 pKa = 3.11ISTPDD227 pKa = 3.27EE228 pKa = 4.34TGLADD233 pKa = 3.99ALANGAIPQPTDD245 pKa = 3.63GNIFWPVDD253 pKa = 3.29EE254 pKa = 5.23ALQLLGVSSLLDD266 pKa = 3.87HH267 pKa = 6.99YY268 pKa = 11.67DD269 pKa = 3.44SGDD272 pKa = 3.23APAIHH277 pKa = 6.51GTSGHH282 pKa = 6.55DD283 pKa = 3.68EE284 pKa = 4.49IIGTSGDD291 pKa = 3.56DD292 pKa = 3.52VIYY295 pKa = 10.83GLGGNDD301 pKa = 3.37TLTGGTADD309 pKa = 4.18DD310 pKa = 5.23FIFGGAGDD318 pKa = 4.87DD319 pKa = 3.78VLSGNAGADD328 pKa = 3.66TLDD331 pKa = 4.33GGAGDD336 pKa = 4.25DD337 pKa = 4.0WLNGGWGWDD346 pKa = 3.54EE347 pKa = 5.17LIGGSGADD355 pKa = 2.82RR356 pKa = 11.84FYY358 pKa = 11.29HH359 pKa = 6.21HH360 pKa = 6.88GVEE363 pKa = 4.38TGFGTEE369 pKa = 4.31WIHH372 pKa = 7.32DD373 pKa = 3.96FSDD376 pKa = 3.76TEE378 pKa = 4.02GDD380 pKa = 3.65RR381 pKa = 11.84LVTGASGTAGDD392 pKa = 4.08FTVSFALAEE401 pKa = 4.26GRR403 pKa = 11.84GSGDD407 pKa = 2.8IAEE410 pKa = 4.29AFIRR414 pKa = 11.84YY415 pKa = 9.03KK416 pKa = 9.9PTGQIAWVLEE426 pKa = 4.48DD427 pKa = 3.97GAGLDD432 pKa = 4.01EE433 pKa = 5.68IRR435 pKa = 11.84IQTADD440 pKa = 3.18GGFDD444 pKa = 3.67LLAA447 pKa = 5.52

Molecular weight: 46.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6X7W1|A0A1M6X7W1_9RHOB Uncharacterized protein OS=Roseovarius pacificus OX=337701 GN=SAMN05444398_101319 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4424

0

4424

1358874

26

2365

307.2

33.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.877 ± 0.048

0.937 ± 0.011

6.184 ± 0.034

6.015 ± 0.036

3.665 ± 0.021

8.744 ± 0.04

2.148 ± 0.015

5.23 ± 0.026

3.064 ± 0.028

10.029 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.849 ± 0.018

2.585 ± 0.018

5.057 ± 0.021

3.242 ± 0.021

6.967 ± 0.036

5.13 ± 0.025

5.463 ± 0.024

7.185 ± 0.029

1.368 ± 0.015

2.26 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski