Vibrio phage fNo16

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Vinavirales; Corticoviridae; unclassified Corticoviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1SVL5|A0A3G1SVL5_9VIRU Uncharacterized protein OS=Vibrio phage fNo16 OX=2315335 GN=fNo16_0003 PE=4 SV=1
MM1 pKa = 7.65IEE3 pKa = 3.78QGIEE7 pKa = 3.76NEE9 pKa = 4.41TIEE12 pKa = 4.44SVEE15 pKa = 3.95VDD17 pKa = 3.21SGEE20 pKa = 4.47LGDD23 pKa = 4.54GADD26 pKa = 4.0SARR29 pKa = 11.84EE30 pKa = 3.9MAQLLAIAKK39 pKa = 9.03EE40 pKa = 3.76IDD42 pKa = 3.5GAQVVPLTEE51 pKa = 4.89DD52 pKa = 2.99EE53 pKa = 4.05QARR56 pKa = 11.84IDD58 pKa = 3.63EE59 pKa = 4.58SEE61 pKa = 4.2RR62 pKa = 11.84VAAIDD67 pKa = 3.69NAAADD72 pKa = 3.79AFEE75 pKa = 4.34AQYY78 pKa = 10.06EE79 pKa = 4.16QAVAIGIDD87 pKa = 3.94ALWTAAAPNWKK98 pKa = 10.18LEE100 pKa = 4.11EE101 pKa = 4.93AEE103 pKa = 4.3LTPMAKK109 pKa = 8.28LTKK112 pKa = 9.68MVIDD116 pKa = 3.29KK117 pKa = 9.86HH118 pKa = 6.02FPNAAEE124 pKa = 4.1KK125 pKa = 9.82MGPEE129 pKa = 4.06VMLGMIALTAISSRR143 pKa = 11.84VAAGIPPRR151 pKa = 11.84GEE153 pKa = 3.52IKK155 pKa = 10.8AKK157 pKa = 10.44GGEE160 pKa = 4.11DD161 pKa = 3.77EE162 pKa = 4.29QTHH165 pKa = 5.55

Molecular weight: 17.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1SVN4|A0A3G1SVN4_9VIRU Uncharacterized protein OS=Vibrio phage fNo16 OX=2315335 GN=fNo16_0018 PE=4 SV=1
MM1 pKa = 7.26FFAIGTRR8 pKa = 11.84FTAAIVTSVTSTRR21 pKa = 11.84GRR23 pKa = 11.84YY24 pKa = 8.85FLIILITYY32 pKa = 7.99VSASRR37 pKa = 11.84RR38 pKa = 11.84EE39 pKa = 4.22QINSRR44 pKa = 11.84ASPDD48 pKa = 3.42VPQLIEE54 pKa = 3.81PP55 pKa = 4.56

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

3250

46

633

141.3

15.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.2 ± 0.862

1.108 ± 0.257

5.385 ± 0.366

6.4 ± 0.743

4.0 ± 0.355

7.877 ± 0.647

1.477 ± 0.304

6.031 ± 0.426

6.338 ± 0.383

7.908 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.284

4.892 ± 0.506

3.908 ± 0.314

4.4 ± 0.434

5.323 ± 0.521

6.308 ± 0.714

5.938 ± 0.583

6.769 ± 0.665

1.292 ± 0.178

2.708 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski