Amino acid dipepetide frequency for Calothrix sp. NIES-4101

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.904AlaAla: 6.904 ± 0.075
0.83AlaCys: 0.83 ± 0.023
3.815AlaAsp: 3.815 ± 0.05
5.007AlaGlu: 5.007 ± 0.06
2.954AlaPhe: 2.954 ± 0.045
5.251AlaGly: 5.251 ± 0.063
1.198AlaHis: 1.198 ± 0.025
7.08AlaIle: 7.08 ± 0.066
4.393AlaLys: 4.393 ± 0.054
7.943AlaLeu: 7.943 ± 0.08
1.649AlaMet: 1.649 ± 0.034
3.901AlaAsn: 3.901 ± 0.062
2.8AlaPro: 2.8 ± 0.047
3.748AlaGln: 3.748 ± 0.045
3.393AlaArg: 3.393 ± 0.046
5.09AlaSer: 5.09 ± 0.054
4.675AlaThr: 4.675 ± 0.055
5.273AlaVal: 5.273 ± 0.059
0.997AlaTrp: 0.997 ± 0.025
2.286AlaTyr: 2.286 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
0.636CysAla: 0.636 ± 0.018
0.165CysCys: 0.165 ± 0.009
0.65CysAsp: 0.65 ± 0.019
0.573CysGlu: 0.573 ± 0.015
0.419CysPhe: 0.419 ± 0.013
0.827CysGly: 0.827 ± 0.021
0.283CysHis: 0.283 ± 0.013
0.649CysIle: 0.649 ± 0.021
0.415CysLys: 0.415 ± 0.014
1.15CysLeu: 1.15 ± 0.026
0.185CysMet: 0.185 ± 0.01
0.438CysAsn: 0.438 ± 0.015
0.506CysPro: 0.506 ± 0.017
0.584CysGln: 0.584 ± 0.016
0.53CysArg: 0.53 ± 0.018
0.612CysSer: 0.612 ± 0.02
0.499CysThr: 0.499 ± 0.014
0.598CysVal: 0.598 ± 0.02
0.145CysTrp: 0.145 ± 0.009
0.343CysTyr: 0.343 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.789AspAla: 3.789 ± 0.05
0.547AspCys: 0.547 ± 0.018
2.182AspAsp: 2.182 ± 0.045
2.949AspGlu: 2.949 ± 0.039
2.389AspPhe: 2.389 ± 0.033
3.335AspGly: 3.335 ± 0.056
0.56AspHis: 0.56 ± 0.017
3.9AspIle: 3.9 ± 0.048
2.639AspLys: 2.639 ± 0.043
5.086AspLeu: 5.086 ± 0.058
0.807AspMet: 0.807 ± 0.022
2.289AspAsn: 2.289 ± 0.039
1.703AspPro: 1.703 ± 0.035
1.225AspGln: 1.225 ± 0.027
2.887AspArg: 2.887 ± 0.045
2.989AspSer: 2.989 ± 0.048
2.568AspThr: 2.568 ± 0.041
3.039AspVal: 3.039 ± 0.044
0.848AspTrp: 0.848 ± 0.021
1.813AspTyr: 1.813 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
4.992GluAla: 4.992 ± 0.064
0.505GluCys: 0.505 ± 0.018
2.618GluAsp: 2.618 ± 0.042
4.047GluGlu: 4.047 ± 0.06
2.539GluPhe: 2.539 ± 0.035
3.041GluGly: 3.041 ± 0.049
0.992GluHis: 0.992 ± 0.023
5.361GluIle: 5.361 ± 0.059
3.973GluLys: 3.973 ± 0.042
6.835GluLeu: 6.835 ± 0.066
1.352GluMet: 1.352 ± 0.027
3.317GluAsn: 3.317 ± 0.049
2.241GluPro: 2.241 ± 0.035
3.249GluGln: 3.249 ± 0.044
3.176GluArg: 3.176 ± 0.044
3.527GluSer: 3.527 ± 0.044
3.422GluThr: 3.422 ± 0.043
4.214GluVal: 4.214 ± 0.045
0.793GluTrp: 0.793 ± 0.022
1.961GluTyr: 1.961 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.353PheAla: 3.353 ± 0.042
0.554PheCys: 0.554 ± 0.019
2.256PheAsp: 2.256 ± 0.041
2.071PheGlu: 2.071 ± 0.033
1.742PhePhe: 1.742 ± 0.035
2.922PheGly: 2.922 ± 0.041
0.757PheHis: 0.757 ± 0.021
2.631PheIle: 2.631 ± 0.039
1.678PheLys: 1.678 ± 0.032
4.242PheLeu: 4.242 ± 0.059
0.73PheMet: 0.73 ± 0.018
1.842PheAsn: 1.842 ± 0.036
1.873PhePro: 1.873 ± 0.031
1.874PheGln: 1.874 ± 0.03
1.853PheArg: 1.853 ± 0.031
3.139PheSer: 3.139 ± 0.044
2.356PheThr: 2.356 ± 0.036
2.514PheVal: 2.514 ± 0.046
0.683PheTrp: 0.683 ± 0.019
1.35PheTyr: 1.35 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
4.568GlyAla: 4.568 ± 0.062
0.828GlyCys: 0.828 ± 0.021
3.278GlyAsp: 3.278 ± 0.05
4.173GlyGlu: 4.173 ± 0.051
2.946GlyPhe: 2.946 ± 0.044
4.585GlyGly: 4.585 ± 0.076
1.095GlyHis: 1.095 ± 0.031
5.354GlyIle: 5.354 ± 0.058
4.631GlyLys: 4.631 ± 0.053
6.449GlyLeu: 6.449 ± 0.067
1.531GlyMet: 1.531 ± 0.03
3.496GlyAsn: 3.496 ± 0.066
0.925GlyPro: 0.925 ± 0.022
2.515GlyGln: 2.515 ± 0.046
2.984GlyArg: 2.984 ± 0.04
3.873GlySer: 3.873 ± 0.053
3.756GlyThr: 3.756 ± 0.061
4.529GlyVal: 4.529 ± 0.057
1.047GlyTrp: 1.047 ± 0.029
2.353GlyTyr: 2.353 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
1.095HisAla: 1.095 ± 0.024
0.252HisCys: 0.252 ± 0.011
0.773HisAsp: 0.773 ± 0.02
1.015HisGlu: 1.015 ± 0.023
0.82HisPhe: 0.82 ± 0.022
1.06HisGly: 1.06 ± 0.025
0.57HisHis: 0.57 ± 0.021
1.177HisIle: 1.177 ± 0.022
0.837HisLys: 0.837 ± 0.02
2.127HisLeu: 2.127 ± 0.032
0.264HisMet: 0.264 ± 0.013
0.811HisAsn: 0.811 ± 0.019
1.313HisPro: 1.313 ± 0.029
1.143HisGln: 1.143 ± 0.024
0.986HisArg: 0.986 ± 0.021
1.172HisSer: 1.172 ± 0.025
0.928HisThr: 0.928 ± 0.023
0.847HisVal: 0.847 ± 0.021
0.323HisTrp: 0.323 ± 0.013
0.663HisTyr: 0.663 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.294IleAla: 7.294 ± 0.057
0.856IleCys: 0.856 ± 0.021
3.928IleAsp: 3.928 ± 0.044
4.388IleGlu: 4.388 ± 0.05
3.022IlePhe: 3.022 ± 0.04
4.559IleGly: 4.559 ± 0.05
1.382IleHis: 1.382 ± 0.025
4.639IleIle: 4.639 ± 0.062
3.547IleLys: 3.547 ± 0.051
7.141IleLeu: 7.141 ± 0.076
1.017IleMet: 1.017 ± 0.02
3.756IleAsn: 3.756 ± 0.048
3.948IlePro: 3.948 ± 0.051
3.337IleGln: 3.337 ± 0.047
3.275IleArg: 3.275 ± 0.044
5.193IleSer: 5.193 ± 0.056
4.212IleThr: 4.212 ± 0.046
4.338IleVal: 4.338 ± 0.059
0.939IleTrp: 0.939 ± 0.026
2.123IleTyr: 2.123 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.942LysAla: 3.942 ± 0.046
0.416LysCys: 0.416 ± 0.015
2.229LysAsp: 2.229 ± 0.04
2.953LysGlu: 2.953 ± 0.041
2.062LysPhe: 2.062 ± 0.032
2.708LysGly: 2.708 ± 0.039
0.926LysHis: 0.926 ± 0.022
4.317LysIle: 4.317 ± 0.056
2.746LysLys: 2.746 ± 0.043
5.895LysLeu: 5.895 ± 0.064
1.12LysMet: 1.12 ± 0.024
2.74LysAsn: 2.74 ± 0.037
2.64LysPro: 2.64 ± 0.037
2.975LysGln: 2.975 ± 0.048
2.479LysArg: 2.479 ± 0.04
3.46LysSer: 3.46 ± 0.045
3.142LysThr: 3.142 ± 0.049
3.309LysVal: 3.309 ± 0.045
0.58LysTrp: 0.58 ± 0.019
1.793LysTyr: 1.793 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
9.112LeuAla: 9.112 ± 0.066
1.028LeuCys: 1.028 ± 0.022
5.002LeuAsp: 5.002 ± 0.058
7.054LeuGlu: 7.054 ± 0.066
3.846LeuPhe: 3.846 ± 0.051
7.201LeuGly: 7.201 ± 0.06
1.87LeuHis: 1.87 ± 0.029
6.992LeuIle: 6.992 ± 0.069
5.447LeuLys: 5.447 ± 0.057
10.74LeuLeu: 10.74 ± 0.096
2.051LeuMet: 2.051 ± 0.039
4.785LeuAsn: 4.785 ± 0.052
5.422LeuPro: 5.422 ± 0.061
5.813LeuGln: 5.813 ± 0.069
5.407LeuArg: 5.407 ± 0.057
7.174LeuSer: 7.174 ± 0.071
6.01LeuThr: 6.01 ± 0.058
6.824LeuVal: 6.824 ± 0.061
1.332LeuTrp: 1.332 ± 0.036
2.581LeuTyr: 2.581 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
1.607MetAla: 1.607 ± 0.032
0.125MetCys: 0.125 ± 0.008
0.699MetAsp: 0.699 ± 0.021
1.004MetGlu: 1.004 ± 0.022
0.63MetPhe: 0.63 ± 0.018
1.371MetGly: 1.371 ± 0.025
0.332MetHis: 0.332 ± 0.013
1.09MetIle: 1.09 ± 0.026
1.073MetLys: 1.073 ± 0.024
1.976MetLeu: 1.976 ± 0.033
0.481MetMet: 0.481 ± 0.018
0.931MetAsn: 0.931 ± 0.024
0.921MetPro: 0.921 ± 0.023
1.03MetGln: 1.03 ± 0.022
1.085MetArg: 1.085 ± 0.027
1.4MetSer: 1.4 ± 0.028
1.302MetThr: 1.302 ± 0.026
1.262MetVal: 1.262 ± 0.028
0.187MetTrp: 0.187 ± 0.01
0.428MetTyr: 0.428 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
3.365AsnAla: 3.365 ± 0.054
0.554AsnCys: 0.554 ± 0.018
1.78AsnAsp: 1.78 ± 0.033
2.078AsnGlu: 2.078 ± 0.035
2.196AsnPhe: 2.196 ± 0.035
2.837AsnGly: 2.837 ± 0.054
1.037AsnHis: 1.037 ± 0.024
3.687AsnIle: 3.687 ± 0.055
2.162AsnLys: 2.162 ± 0.032
6.051AsnLeu: 6.051 ± 0.078
0.748AsnMet: 0.748 ± 0.02
2.772AsnAsn: 2.772 ± 0.056
3.217AsnPro: 3.217 ± 0.044
3.087AsnGln: 3.087 ± 0.05
2.493AsnArg: 2.493 ± 0.039
3.664AsnSer: 3.664 ± 0.057
2.66AsnThr: 2.66 ± 0.046
2.428AsnVal: 2.428 ± 0.036
0.828AsnTrp: 0.828 ± 0.023
1.794AsnTyr: 1.794 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
3.132ProAla: 3.132 ± 0.048
0.338ProCys: 0.338 ± 0.012
2.647ProAsp: 2.647 ± 0.039
3.792ProGlu: 3.792 ± 0.049
1.64ProPhe: 1.64 ± 0.027
2.983ProGly: 2.983 ± 0.042
0.866ProHis: 0.866 ± 0.017
3.09ProIle: 3.09 ± 0.044
2.289ProLys: 2.289 ± 0.035
4.295ProLeu: 4.295 ± 0.047
0.717ProMet: 0.717 ± 0.016
2.408ProAsn: 2.408 ± 0.038
2.074ProPro: 2.074 ± 0.044
2.568ProGln: 2.568 ± 0.041
1.623ProArg: 1.623 ± 0.031
2.837ProSer: 2.837 ± 0.042
2.769ProThr: 2.769 ± 0.048
3.108ProVal: 3.108 ± 0.045
0.563ProTrp: 0.563 ± 0.017
1.194ProTyr: 1.194 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.578GlnAla: 4.578 ± 0.049
0.351GlnCys: 0.351 ± 0.015
2.086GlnAsp: 2.086 ± 0.033
3.629GlnGlu: 3.629 ± 0.054
1.727GlnPhe: 1.727 ± 0.031
3.112GlnGly: 3.112 ± 0.043
0.869GlnHis: 0.869 ± 0.02
3.936GlnIle: 3.936 ± 0.05
3.08GlnLys: 3.08 ± 0.047
5.509GlnLeu: 5.509 ± 0.067
1.046GlnMet: 1.046 ± 0.024
2.449GlnAsn: 2.449 ± 0.041
2.436GlnPro: 2.436 ± 0.045
3.708GlnGln: 3.708 ± 0.064
2.77GlnArg: 2.77 ± 0.044
2.884GlnSer: 2.884 ± 0.036
2.952GlnThr: 2.952 ± 0.042
3.739GlnVal: 3.739 ± 0.05
0.621GlnTrp: 0.621 ± 0.022
1.281GlnTyr: 1.281 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
3.03ArgAla: 3.03 ± 0.048
0.485ArgCys: 0.485 ± 0.016
2.652ArgAsp: 2.652 ± 0.04
3.321ArgGlu: 3.321 ± 0.042
2.124ArgPhe: 2.124 ± 0.034
2.941ArgGly: 2.941 ± 0.049
1.04ArgHis: 1.04 ± 0.022
3.451ArgIle: 3.451 ± 0.044
2.479ArgLys: 2.479 ± 0.039
5.395ArgLeu: 5.395 ± 0.066
1.009ArgMet: 1.009 ± 0.022
2.415ArgAsn: 2.415 ± 0.03
1.767ArgPro: 1.767 ± 0.031
2.99ArgGln: 2.99 ± 0.042
2.836ArgArg: 2.836 ± 0.048
3.071ArgSer: 3.071 ± 0.039
2.452ArgThr: 2.452 ± 0.033
3.207ArgVal: 3.207 ± 0.043
0.726ArgTrp: 0.726 ± 0.023
1.839ArgTyr: 1.839 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
4.573SerAla: 4.573 ± 0.051
0.643SerCys: 0.643 ± 0.02
3.164SerAsp: 3.164 ± 0.038
3.846SerGlu: 3.846 ± 0.048
2.643SerPhe: 2.643 ± 0.038
4.665SerGly: 4.665 ± 0.056
1.435SerHis: 1.435 ± 0.027
4.222SerIle: 4.222 ± 0.046
3.005SerLys: 3.005 ± 0.044
7.457SerLeu: 7.457 ± 0.072
1.179SerMet: 1.179 ± 0.027
3.162SerAsn: 3.162 ± 0.05
3.402SerPro: 3.402 ± 0.045
4.068SerGln: 4.068 ± 0.048
3.284SerArg: 3.284 ± 0.039
4.714SerSer: 4.714 ± 0.061
3.593SerThr: 3.593 ± 0.049
3.958SerVal: 3.958 ± 0.049
0.897SerTrp: 0.897 ± 0.021
1.836SerTyr: 1.836 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
4.642ThrAla: 4.642 ± 0.055
0.488ThrCys: 0.488 ± 0.017
2.546ThrAsp: 2.546 ± 0.041
3.281ThrGlu: 3.281 ± 0.045
2.175ThrPhe: 2.175 ± 0.033
4.312ThrGly: 4.312 ± 0.06
0.985ThrHis: 0.985 ± 0.024
3.985ThrIle: 3.985 ± 0.054
2.598ThrLys: 2.598 ± 0.041
5.929ThrLeu: 5.929 ± 0.062
0.793ThrMet: 0.793 ± 0.021
2.75ThrAsn: 2.75 ± 0.047
3.38ThrPro: 3.38 ± 0.057
2.953ThrGln: 2.953 ± 0.041
2.342ThrArg: 2.342 ± 0.036
3.841ThrSer: 3.841 ± 0.046
3.499ThrThr: 3.499 ± 0.057
3.967ThrVal: 3.967 ± 0.046
0.727ThrTrp: 0.727 ± 0.019
1.585ThrTyr: 1.585 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
5.62ValAla: 5.62 ± 0.059
0.668ValCys: 0.668 ± 0.017
3.191ValAsp: 3.191 ± 0.042
4.348ValGlu: 4.348 ± 0.047
2.539ValPhe: 2.539 ± 0.039
4.27ValGly: 4.27 ± 0.049
0.94ValHis: 0.94 ± 0.022
4.604ValIle: 4.604 ± 0.056
3.417ValLys: 3.417 ± 0.045
6.134ValLeu: 6.134 ± 0.062
1.43ValMet: 1.43 ± 0.031
3.238ValAsn: 3.238 ± 0.05
2.669ValPro: 2.669 ± 0.039
2.674ValGln: 2.674 ± 0.037
3.113ValArg: 3.113 ± 0.048
4.276ValSer: 4.276 ± 0.042
3.87ValThr: 3.87 ± 0.053
4.627ValVal: 4.627 ± 0.055
0.794ValTrp: 0.794 ± 0.02
1.789ValTyr: 1.789 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.851TrpAla: 0.851 ± 0.021
0.163TrpCys: 0.163 ± 0.009
0.682TrpAsp: 0.682 ± 0.022
0.947TrpGlu: 0.947 ± 0.024
0.597TrpPhe: 0.597 ± 0.019
0.95TrpGly: 0.95 ± 0.025
0.36TrpHis: 0.36 ± 0.013
0.803TrpIle: 0.803 ± 0.021
0.676TrpLys: 0.676 ± 0.02
1.79TrpLeu: 1.79 ± 0.034
0.328TrpMet: 0.328 ± 0.014
0.638TrpAsn: 0.638 ± 0.018
0.098TrpPro: 0.098 ± 0.007
1.163TrpGln: 1.163 ± 0.028
0.818TrpArg: 0.818 ± 0.022
0.803TrpSer: 0.803 ± 0.024
0.566TrpThr: 0.566 ± 0.018
0.885TrpVal: 0.885 ± 0.023
0.25TrpTrp: 0.25 ± 0.014
0.423TrpTyr: 0.423 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.101TyrAla: 2.101 ± 0.035
0.413TyrCys: 0.413 ± 0.013
1.363TyrAsp: 1.363 ± 0.025
1.679TyrGlu: 1.679 ± 0.033
1.408TyrPhe: 1.408 ± 0.031
1.988TyrGly: 1.988 ± 0.032
0.682TyrHis: 0.682 ± 0.02
1.926TyrIle: 1.926 ± 0.032
1.35TyrLys: 1.35 ± 0.029
3.543TyrLeu: 3.543 ± 0.043
0.498TyrMet: 0.498 ± 0.017
1.298TyrAsn: 1.298 ± 0.03
1.6TyrPro: 1.6 ± 0.028
2.06TyrGln: 2.06 ± 0.04
1.895TyrArg: 1.895 ± 0.036
2.028TyrSer: 2.028 ± 0.039
1.545TyrThr: 1.545 ± 0.03
1.545TyrVal: 1.545 ± 0.03
0.511TyrTrp: 0.511 ± 0.013
1.117TyrTyr: 1.117 ± 0.028
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6218 proteins (1974457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski