Amino acid dipepetide frequency for Microbacterium testaceum (strain StLB037)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.373AlaAla: 21.373 ± 0.233	0.678AlaCys: 0.678 ± 0.026	8.716AlaAsp: 8.716 ± 0.112	8.187AlaGlu: 8.187 ± 0.1	4.174AlaPhe: 4.174 ± 0.067	12.217AlaGly: 12.217 ± 0.111	2.618AlaHis: 2.618 ± 0.044	5.595AlaIle: 5.595 ± 0.077	2.432AlaLys: 2.432 ± 0.057	14.765AlaLeu: 14.765 ± 0.151	2.62AlaMet: 2.62 ± 0.047	2.205AlaAsn: 2.205 ± 0.049	7.24AlaPro: 7.24 ± 0.102	3.678AlaGln: 3.678 ± 0.062	10.272AlaArg: 10.272 ± 0.115	7.9AlaSer: 7.9 ± 0.094	7.786AlaThr: 7.786 ± 0.078	12.242AlaVal: 12.242 ± 0.136	2.085AlaTrp: 2.085 ± 0.044	2.473AlaTyr: 2.473 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.687CysAla: 0.687 ± 0.026	0.044CysCys: 0.044 ± 0.007	0.308CysAsp: 0.308 ± 0.018	0.209CysGlu: 0.209 ± 0.013	0.154CysPhe: 0.154 ± 0.009	0.522CysGly: 0.522 ± 0.023	0.103CysHis: 0.103 ± 0.008	0.147CysIle: 0.147 ± 0.012	0.056CysLys: 0.056 ± 0.007	0.423CysLeu: 0.423 ± 0.021	0.07CysMet: 0.07 ± 0.007	0.096CysAsn: 0.096 ± 0.009	0.257CysPro: 0.257 ± 0.017	0.115CysGln: 0.115 ± 0.011	0.29CysArg: 0.29 ± 0.016	0.297CysSer: 0.297 ± 0.016	0.3CysThr: 0.3 ± 0.016	0.416CysVal: 0.416 ± 0.018	0.066CysTrp: 0.066 ± 0.009	0.087CysTyr: 0.087 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	10.372AspAla: 10.372 ± 0.123	0.21AspCys: 0.21 ± 0.013	4.916AspAsp: 4.916 ± 0.069	4.326AspGlu: 4.326 ± 0.075	1.62AspPhe: 1.62 ± 0.038	6.289AspGly: 6.289 ± 0.085	1.15AspHis: 1.15 ± 0.029	2.416AspIle: 2.416 ± 0.043	0.938AspLys: 0.938 ± 0.033	6.499AspLeu: 6.499 ± 0.084	0.8AspMet: 0.8 ± 0.025	0.984AspAsn: 0.984 ± 0.032	4.327AspPro: 4.327 ± 0.066	1.439AspGln: 1.439 ± 0.037	4.623AspArg: 4.623 ± 0.077	2.387AspSer: 2.387 ± 0.05	3.14AspThr: 3.14 ± 0.052	5.76AspVal: 5.76 ± 0.07	0.908AspTrp: 0.908 ± 0.032	1.248AspTyr: 1.248 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.998GluAla: 6.998 ± 0.097	0.21GluCys: 0.21 ± 0.013	2.446GluAsp: 2.446 ± 0.046	2.768GluGlu: 2.768 ± 0.054	1.491GluPhe: 1.491 ± 0.04	4.197GluGly: 4.197 ± 0.056	1.303GluHis: 1.303 ± 0.038	2.671GluIle: 2.671 ± 0.053	1.336GluLys: 1.336 ± 0.039	5.731GluLeu: 5.731 ± 0.073	0.868GluMet: 0.868 ± 0.028	1.271GluAsn: 1.271 ± 0.033	3.003GluPro: 3.003 ± 0.058	1.847GluGln: 1.847 ± 0.041	5.345GluArg: 5.345 ± 0.088	2.605GluSer: 2.605 ± 0.052	3.118GluThr: 3.118 ± 0.051	4.641GluVal: 4.641 ± 0.066	0.886GluTrp: 0.886 ± 0.029	1.074GluTyr: 1.074 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.458PheAla: 4.458 ± 0.061	0.147PheCys: 0.147 ± 0.011	2.432PheAsp: 2.432 ± 0.046	1.517PheGlu: 1.517 ± 0.039	1.185PhePhe: 1.185 ± 0.037	3.37PheGly: 3.37 ± 0.056	0.57PheHis: 0.57 ± 0.024	1.063PheIle: 1.063 ± 0.032	0.376PheLys: 0.376 ± 0.021	2.971PheLeu: 2.971 ± 0.064	0.36PheMet: 0.36 ± 0.017	0.573PheAsn: 0.573 ± 0.023	1.461PhePro: 1.461 ± 0.032	0.754PheGln: 0.754 ± 0.027	1.841PheArg: 1.841 ± 0.04	1.857PheSer: 1.857 ± 0.037	2.087PheThr: 2.087 ± 0.043	3.024PheVal: 3.024 ± 0.051	0.48PheTrp: 0.48 ± 0.023	0.627PheTyr: 0.627 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	10.979GlyAla: 10.979 ± 0.103	0.561GlyCys: 0.561 ± 0.024	5.247GlyAsp: 5.247 ± 0.07	4.857GlyGlu: 4.857 ± 0.065	3.263GlyPhe: 3.263 ± 0.058	7.899GlyGly: 7.899 ± 0.09	1.749GlyHis: 1.749 ± 0.037	4.901GlyIle: 4.901 ± 0.063	1.868GlyLys: 1.868 ± 0.046	8.675GlyLeu: 8.675 ± 0.102	1.819GlyMet: 1.819 ± 0.041	1.638GlyAsn: 1.638 ± 0.045	4.061GlyPro: 4.061 ± 0.063	2.316GlyGln: 2.316 ± 0.049	6.56GlyArg: 6.56 ± 0.09	5.389GlySer: 5.389 ± 0.077	5.645GlyThr: 5.645 ± 0.089	8.305GlyVal: 8.305 ± 0.089	1.745GlyTrp: 1.745 ± 0.042	2.185GlyTyr: 2.185 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.426HisAla: 2.426 ± 0.046	0.105HisCys: 0.105 ± 0.009	1.298HisAsp: 1.298 ± 0.039	1.033HisGlu: 1.033 ± 0.03	0.506HisPhe: 0.506 ± 0.022	1.899HisGly: 1.899 ± 0.04	0.511HisHis: 0.511 ± 0.023	0.67HisIle: 0.67 ± 0.022	0.23HisLys: 0.23 ± 0.014	2.002HisLeu: 2.002 ± 0.044	0.281HisMet: 0.281 ± 0.016	0.322HisAsn: 0.322 ± 0.016	1.514HisPro: 1.514 ± 0.04	0.405HisGln: 0.405 ± 0.019	1.631HisArg: 1.631 ± 0.04	0.892HisSer: 0.892 ± 0.026	0.992HisThr: 0.992 ± 0.028	1.758HisVal: 1.758 ± 0.035	0.275HisTrp: 0.275 ± 0.015	0.393HisTyr: 0.393 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.806IleAla: 6.806 ± 0.082	0.202IleCys: 0.202 ± 0.014	3.461IleAsp: 3.461 ± 0.056	2.572IleGlu: 2.572 ± 0.052	1.116IlePhe: 1.116 ± 0.033	4.341IleGly: 4.341 ± 0.063	0.655IleHis: 0.655 ± 0.024	1.501IleIle: 1.501 ± 0.044	0.603IleLys: 0.603 ± 0.021	3.536IleLeu: 3.536 ± 0.054	0.536IleMet: 0.536 ± 0.024	0.785IleAsn: 0.785 ± 0.028	2.412IlePro: 2.412 ± 0.049	0.912IleGln: 0.912 ± 0.024	2.615IleArg: 2.615 ± 0.051	2.167IleSer: 2.167 ± 0.042	2.575IleThr: 2.575 ± 0.046	4.703IleVal: 4.703 ± 0.072	0.462IleTrp: 0.462 ± 0.02	0.646IleTyr: 0.646 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	2.119LysAla: 2.119 ± 0.052	0.059LysCys: 0.059 ± 0.007	1.046LysAsp: 1.046 ± 0.036	0.838LysGlu: 0.838 ± 0.028	0.434LysPhe: 0.434 ± 0.022	1.43LysGly: 1.43 ± 0.044	0.344LysHis: 0.344 ± 0.018	0.807LysIle: 0.807 ± 0.028	0.715LysLys: 0.715 ± 0.031	1.644LysLeu: 1.644 ± 0.04	0.307LysMet: 0.307 ± 0.017	0.504LysAsn: 0.504 ± 0.021	1.021LysPro: 1.021 ± 0.033	0.566LysGln: 0.566 ± 0.021	1.317LysArg: 1.317 ± 0.036	0.911LysSer: 0.911 ± 0.032	1.195LysThr: 1.195 ± 0.037	1.498LysVal: 1.498 ± 0.045	0.224LysTrp: 0.224 ± 0.013	0.413LysTyr: 0.413 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.79LeuAla: 14.79 ± 0.133	0.432LeuCys: 0.432 ± 0.02	6.85LeuAsp: 6.85 ± 0.083	4.887LeuGlu: 4.887 ± 0.07	2.946LeuPhe: 2.946 ± 0.061	8.929LeuGly: 8.929 ± 0.109	1.774LeuHis: 1.774 ± 0.041	4.071LeuIle: 4.071 ± 0.066	1.47LeuLys: 1.47 ± 0.038	9.858LeuLeu: 9.858 ± 0.116	1.437LeuMet: 1.437 ± 0.038	1.65LeuAsn: 1.65 ± 0.039	5.703LeuPro: 5.703 ± 0.071	2.371LeuGln: 2.371 ± 0.05	7.787LeuArg: 7.787 ± 0.102	6.245LeuSer: 6.245 ± 0.077	6.349LeuThr: 6.349 ± 0.081	9.361LeuVal: 9.361 ± 0.111	1.328LeuTrp: 1.328 ± 0.036	1.563LeuTyr: 1.563 ± 0.034	0.0LeuXaa: 0.0 ± 0.0
Met	1.929MetAla: 1.929 ± 0.045	0.096MetCys: 0.096 ± 0.009	0.77MetAsp: 0.77 ± 0.026	0.534MetGlu: 0.534 ± 0.019	0.491MetPhe: 0.491 ± 0.022	1.223MetGly: 1.223 ± 0.032	0.293MetHis: 0.293 ± 0.016	0.791MetIle: 0.791 ± 0.027	0.375MetLys: 0.375 ± 0.017	1.602MetLeu: 1.602 ± 0.039	0.283MetMet: 0.283 ± 0.017	0.443MetAsn: 0.443 ± 0.02	1.108MetPro: 1.108 ± 0.026	0.477MetGln: 0.477 ± 0.02	1.249MetArg: 1.249 ± 0.031	1.475MetSer: 1.475 ± 0.035	1.769MetThr: 1.769 ± 0.037	1.195MetVal: 1.195 ± 0.036	0.214MetTrp: 0.214 ± 0.011	0.273MetTyr: 0.273 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.495AsnAla: 2.495 ± 0.059	0.088AsnCys: 0.088 ± 0.009	1.208AsnAsp: 1.208 ± 0.033	0.866AsnGlu: 0.866 ± 0.027	0.594AsnPhe: 0.594 ± 0.021	1.952AsnGly: 1.952 ± 0.047	0.355AsnHis: 0.355 ± 0.016	0.782AsnIle: 0.782 ± 0.025	0.33AsnLys: 0.33 ± 0.018	1.777AsnLeu: 1.777 ± 0.038	0.281AsnMet: 0.281 ± 0.016	0.462AsnAsn: 0.462 ± 0.021	1.477AsnPro: 1.477 ± 0.039	0.511AsnGln: 0.511 ± 0.024	1.14AsnArg: 1.14 ± 0.03	0.898AsnSer: 0.898 ± 0.03	1.159AsnThr: 1.159 ± 0.035	1.619AsnVal: 1.619 ± 0.039	0.282AsnTrp: 0.282 ± 0.016	0.434AsnTyr: 0.434 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.85ProAla: 7.85 ± 0.112	0.177ProCys: 0.177 ± 0.011	4.184ProAsp: 4.184 ± 0.066	3.728ProGlu: 3.728 ± 0.062	1.823ProPhe: 1.823 ± 0.04	5.243ProGly: 5.243 ± 0.07	1.138ProHis: 1.138 ± 0.033	1.942ProIle: 1.942 ± 0.041	0.874ProLys: 0.874 ± 0.026	5.176ProLeu: 5.176 ± 0.06	0.843ProMet: 0.843 ± 0.027	0.98ProAsn: 0.98 ± 0.028	2.551ProPro: 2.551 ± 0.064	1.375ProGln: 1.375 ± 0.03	3.937ProArg: 3.937 ± 0.065	3.312ProSer: 3.312 ± 0.05	3.605ProThr: 3.605 ± 0.064	5.267ProVal: 5.267 ± 0.075	0.954ProTrp: 0.954 ± 0.03	1.037ProTyr: 1.037 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.155GlnAla: 3.155 ± 0.056	0.112GlnCys: 0.112 ± 0.009	1.216GlnAsp: 1.216 ± 0.031	1.035GlnGlu: 1.035 ± 0.031	0.792GlnPhe: 0.792 ± 0.028	1.955GlnGly: 1.955 ± 0.045	0.544GlnHis: 0.544 ± 0.02	1.25GlnIle: 1.25 ± 0.035	0.66GlnLys: 0.66 ± 0.026	2.843GlnLeu: 2.843 ± 0.06	0.444GlnMet: 0.444 ± 0.018	0.675GlnAsn: 0.675 ± 0.027	1.409GlnPro: 1.409 ± 0.036	1.037GlnGln: 1.037 ± 0.036	2.279GlnArg: 2.279 ± 0.049	1.458GlnSer: 1.458 ± 0.037	1.55GlnThr: 1.55 ± 0.037	2.152GlnVal: 2.152 ± 0.045	0.49GlnTrp: 0.49 ± 0.019	0.58GlnTyr: 0.58 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	9.996ArgAla: 9.996 ± 0.107	0.307ArgCys: 0.307 ± 0.016	4.6ArgAsp: 4.6 ± 0.078	4.536ArgGlu: 4.536 ± 0.074	2.442ArgPhe: 2.442 ± 0.042	5.979ArgGly: 5.979 ± 0.082	1.6ArgHis: 1.6 ± 0.041	3.406ArgIle: 3.406 ± 0.061	1.163ArgLys: 1.163 ± 0.038	7.522ArgLeu: 7.522 ± 0.091	1.652ArgMet: 1.652 ± 0.038	1.246ArgAsn: 1.246 ± 0.034	3.917ArgPro: 3.917 ± 0.059	1.916ArgGln: 1.916 ± 0.048	7.222ArgArg: 7.222 ± 0.104	4.136ArgSer: 4.136 ± 0.058	4.491ArgThr: 4.491 ± 0.068	6.791ArgVal: 6.791 ± 0.078	1.349ArgTrp: 1.349 ± 0.035	1.496ArgTyr: 1.496 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.569SerAla: 7.569 ± 0.088	0.288SerCys: 0.288 ± 0.018	3.421SerAsp: 3.421 ± 0.057	2.522SerGlu: 2.522 ± 0.05	2.035SerPhe: 2.035 ± 0.041	5.825SerGly: 5.825 ± 0.091	0.954SerHis: 0.954 ± 0.031	2.375SerIle: 2.375 ± 0.051	0.864SerLys: 0.864 ± 0.028	5.428SerLeu: 5.428 ± 0.079	1.11SerMet: 1.11 ± 0.029	1.04SerAsn: 1.04 ± 0.027	3.44SerPro: 3.44 ± 0.057	1.256SerGln: 1.256 ± 0.032	3.952SerArg: 3.952 ± 0.064	3.612SerSer: 3.612 ± 0.072	3.857SerThr: 3.857 ± 0.061	4.871SerVal: 4.871 ± 0.077	0.997SerTrp: 0.997 ± 0.031	1.167SerTyr: 1.167 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	8.334ThrAla: 8.334 ± 0.092	0.265ThrCys: 0.265 ± 0.014	3.832ThrAsp: 3.832 ± 0.057	2.778ThrGlu: 2.778 ± 0.046	1.971ThrPhe: 1.971 ± 0.04	5.751ThrGly: 5.751 ± 0.078	1.092ThrHis: 1.092 ± 0.032	2.636ThrIle: 2.636 ± 0.048	1.035ThrLys: 1.035 ± 0.036	6.365ThrLeu: 6.365 ± 0.076	0.867ThrMet: 0.867 ± 0.03	1.176ThrAsn: 1.176 ± 0.039	4.381ThrPro: 4.381 ± 0.075	1.424ThrGln: 1.424 ± 0.035	4.021ThrArg: 4.021 ± 0.063	3.517ThrSer: 3.517 ± 0.062	3.993ThrThr: 3.993 ± 0.067	6.26ThrVal: 6.26 ± 0.086	0.958ThrTrp: 0.958 ± 0.031	1.228ThrTyr: 1.228 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	12.653ValAla: 12.653 ± 0.125	0.472ValCys: 0.472 ± 0.021	6.125ValAsp: 6.125 ± 0.068	4.93ValGlu: 4.93 ± 0.072	2.892ValPhe: 2.892 ± 0.049	7.461ValGly: 7.461 ± 0.09	1.78ValHis: 1.78 ± 0.042	4.185ValIle: 4.185 ± 0.071	1.448ValLys: 1.448 ± 0.036	9.384ValLeu: 9.384 ± 0.104	1.368ValMet: 1.368 ± 0.037	1.718ValAsn: 1.718 ± 0.039	4.961ValPro: 4.961 ± 0.066	2.199ValGln: 2.199 ± 0.049	6.73ValArg: 6.73 ± 0.087	5.481ValSer: 5.481 ± 0.079	6.021ValThr: 6.021 ± 0.085	9.463ValVal: 9.463 ± 0.101	1.3ValTrp: 1.3 ± 0.036	1.54ValTyr: 1.54 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.719TrpAla: 1.719 ± 0.04	0.082TrpCys: 0.082 ± 0.008	0.907TrpAsp: 0.907 ± 0.029	0.672TrpGlu: 0.672 ± 0.025	0.575TrpPhe: 0.575 ± 0.023	1.301TrpGly: 1.301 ± 0.044	0.35TrpHis: 0.35 ± 0.015	0.744TrpIle: 0.744 ± 0.027	0.308TrpLys: 0.308 ± 0.019	1.621TrpLeu: 1.621 ± 0.038	0.386TrpMet: 0.386 ± 0.019	0.467TrpAsn: 0.467 ± 0.022	0.768TrpPro: 0.768 ± 0.026	0.533TrpGln: 0.533 ± 0.023	1.309TrpArg: 1.309 ± 0.035	0.986TrpSer: 0.986 ± 0.03	1.075TrpThr: 1.075 ± 0.039	1.208TrpVal: 1.208 ± 0.032	0.366TrpTrp: 0.366 ± 0.021	0.304TrpTyr: 0.304 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.547TyrAla: 2.547 ± 0.042	0.11TyrCys: 0.11 ± 0.009	1.373TyrAsp: 1.373 ± 0.044	1.012TyrGlu: 1.012 ± 0.028	0.646TyrPhe: 0.646 ± 0.026	1.88TyrGly: 1.88 ± 0.048	0.262TyrHis: 0.262 ± 0.015	0.651TyrIle: 0.651 ± 0.028	0.312TyrLys: 0.312 ± 0.017	1.979TyrLeu: 1.979 ± 0.04	0.26TyrMet: 0.26 ± 0.015	0.477TyrAsn: 0.477 ± 0.025	0.983TyrPro: 0.983 ± 0.03	0.506TyrGln: 0.506 ± 0.023	1.644TyrArg: 1.644 ± 0.039	1.059TyrSer: 1.059 ± 0.034	1.177TyrThr: 1.177 ± 0.035	1.578TyrVal: 1.578 ± 0.035	0.313TyrTrp: 0.313 ± 0.015	0.447TyrTyr: 0.447 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3671 proteins (1212999 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---