Amino acid dipepetide frequency for Eubacterium sp. CAG:581

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.077AlaAla: 3.077 ± 0.113	0.954AlaCys: 0.954 ± 0.046	3.644AlaAsp: 3.644 ± 0.098	3.916AlaGlu: 3.916 ± 0.116	2.768AlaPhe: 2.768 ± 0.078	4.209AlaGly: 4.209 ± 0.091	0.88AlaHis: 0.88 ± 0.042	5.327AlaIle: 5.327 ± 0.108	5.392AlaLys: 5.392 ± 0.111	5.616AlaLeu: 5.616 ± 0.119	1.894AlaMet: 1.894 ± 0.055	2.989AlaAsn: 2.989 ± 0.084	1.694AlaPro: 1.694 ± 0.073	1.531AlaGln: 1.531 ± 0.06	1.961AlaArg: 1.961 ± 0.068	3.511AlaSer: 3.511 ± 0.087	4.099AlaThr: 4.099 ± 0.114	5.146AlaVal: 5.146 ± 0.112	0.339AlaTrp: 0.339 ± 0.025	2.334AlaTyr: 2.334 ± 0.059	0.002AlaXaa: 0.002 ± 0.002
Cys	0.921CysAla: 0.921 ± 0.045	0.337CysCys: 0.337 ± 0.03	1.078CysAsp: 1.078 ± 0.047	0.96CysGlu: 0.96 ± 0.044	0.659CysPhe: 0.659 ± 0.033	1.614CysGly: 1.614 ± 0.066	0.329CysHis: 0.329 ± 0.024	1.076CysIle: 1.076 ± 0.047	1.384CysLys: 1.384 ± 0.061	1.119CysLeu: 1.119 ± 0.046	0.341CysMet: 0.341 ± 0.028	0.902CysAsn: 0.902 ± 0.046	0.624CysPro: 0.624 ± 0.035	0.324CysGln: 0.324 ± 0.025	0.463CysArg: 0.463 ± 0.03	1.1CysSer: 1.1 ± 0.053	0.843CysThr: 0.843 ± 0.041	1.085CysVal: 1.085 ± 0.046	0.088CysTrp: 0.088 ± 0.013	0.718CysTyr: 0.718 ± 0.034	0.0CysXaa: 0.0 ± 0.0
Asp	3.282AspAla: 3.282 ± 0.093	0.933AspCys: 0.933 ± 0.042	3.592AspAsp: 3.592 ± 0.09	4.689AspGlu: 4.689 ± 0.11	3.177AspPhe: 3.177 ± 0.08	4.23AspGly: 4.23 ± 0.092	0.723AspHis: 0.723 ± 0.042	5.197AspIle: 5.197 ± 0.102	5.748AspLys: 5.748 ± 0.109	4.474AspLeu: 4.474 ± 0.097	1.71AspMet: 1.71 ± 0.056	3.819AspAsn: 3.819 ± 0.083	1.382AspPro: 1.382 ± 0.054	0.876AspGln: 0.876 ± 0.043	1.908AspArg: 1.908 ± 0.066	3.851AspSer: 3.851 ± 0.103	3.733AspThr: 3.733 ± 0.087	3.895AspVal: 3.895 ± 0.101	0.407AspTrp: 0.407 ± 0.029	3.13AspTyr: 3.13 ± 0.081	0.004AspXaa: 0.004 ± 0.002
Glu	3.396GluAla: 3.396 ± 0.103	0.857GluCys: 0.857 ± 0.043	3.857GluAsp: 3.857 ± 0.106	5.422GluGlu: 5.422 ± 0.134	2.473GluPhe: 2.473 ± 0.07	3.375GluGly: 3.375 ± 0.093	0.904GluHis: 0.904 ± 0.046	5.666GluIle: 5.666 ± 0.113	7.0GluLys: 7.0 ± 0.149	5.361GluLeu: 5.361 ± 0.131	2.056GluMet: 2.056 ± 0.073	5.011GluAsn: 5.011 ± 0.102	1.538GluPro: 1.538 ± 0.067	1.811GluGln: 1.811 ± 0.06	2.298GluArg: 2.298 ± 0.063	3.255GluSer: 3.255 ± 0.084	3.358GluThr: 3.358 ± 0.091	4.166GluVal: 4.166 ± 0.092	0.36GluTrp: 0.36 ± 0.026	2.856GluTyr: 2.856 ± 0.078	0.0GluXaa: 0.0 ± 0.0
Phe	2.875PheAla: 2.875 ± 0.082	0.826PheCys: 0.826 ± 0.041	2.907PheAsp: 2.907 ± 0.075	2.323PheGlu: 2.323 ± 0.072	1.915PhePhe: 1.915 ± 0.072	3.077PheGly: 3.077 ± 0.086	0.653PheHis: 0.653 ± 0.03	3.122PheIle: 3.122 ± 0.094	2.995PheLys: 2.995 ± 0.073	3.6PheLeu: 3.6 ± 0.099	1.135PheMet: 1.135 ± 0.052	2.521PheAsn: 2.521 ± 0.071	1.319PhePro: 1.319 ± 0.056	1.03PheGln: 1.03 ± 0.044	1.321PheArg: 1.321 ± 0.054	3.303PheSer: 3.303 ± 0.093	2.879PheThr: 2.879 ± 0.075	3.259PheVal: 3.259 ± 0.085	0.289PheTrp: 0.289 ± 0.027	1.755PheTyr: 1.755 ± 0.061	0.0PheXaa: 0.0 ± 0.0
Gly	4.394GlyAla: 4.394 ± 0.112	1.205GlyCys: 1.205 ± 0.046	3.863GlyAsp: 3.863 ± 0.093	4.219GlyGlu: 4.219 ± 0.088	2.877GlyPhe: 2.877 ± 0.085	4.428GlyGly: 4.428 ± 0.117	1.019GlyHis: 1.019 ± 0.049	5.942GlyIle: 5.942 ± 0.129	5.955GlyLys: 5.955 ± 0.119	5.041GlyLeu: 5.041 ± 0.111	1.97GlyMet: 1.97 ± 0.066	3.431GlyAsn: 3.431 ± 0.095	1.02GlyPro: 1.02 ± 0.05	1.552GlyGln: 1.552 ± 0.059	2.25GlyArg: 2.25 ± 0.071	3.888GlySer: 3.888 ± 0.099	4.192GlyThr: 4.192 ± 0.106	5.283GlyVal: 5.283 ± 0.102	0.514GlyTrp: 0.514 ± 0.04	3.196GlyTyr: 3.196 ± 0.085	0.004GlyXaa: 0.004 ± 0.003
His	0.725HisAla: 0.725 ± 0.038	0.341HisCys: 0.341 ± 0.027	0.655HisAsp: 0.655 ± 0.041	0.634HisGlu: 0.634 ± 0.033	0.763HisPhe: 0.763 ± 0.037	0.998HisGly: 0.998 ± 0.047	0.367HisHis: 0.367 ± 0.036	1.283HisIle: 1.283 ± 0.045	1.218HisLys: 1.218 ± 0.048	1.213HisLeu: 1.213 ± 0.046	0.396HisMet: 0.396 ± 0.028	1.062HisAsn: 1.062 ± 0.044	0.695HisPro: 0.695 ± 0.039	0.411HisGln: 0.411 ± 0.026	0.613HisArg: 0.613 ± 0.031	1.003HisSer: 1.003 ± 0.05	0.935HisThr: 0.935 ± 0.044	0.562HisVal: 0.562 ± 0.039	0.12HisTrp: 0.12 ± 0.015	0.796HisTyr: 0.796 ± 0.044	0.002HisXaa: 0.002 ± 0.002
Ile	5.544IleAla: 5.544 ± 0.102	1.5IleCys: 1.5 ± 0.056	5.249IleAsp: 5.249 ± 0.094	4.881IleGlu: 4.881 ± 0.104	3.419IlePhe: 3.419 ± 0.106	5.043IleGly: 5.043 ± 0.098	1.112IleHis: 1.112 ± 0.055	6.707IleIle: 6.707 ± 0.138	6.566IleLys: 6.566 ± 0.121	6.669IleLeu: 6.669 ± 0.134	2.077IleMet: 2.077 ± 0.072	4.64IleAsn: 4.64 ± 0.104	2.957IlePro: 2.957 ± 0.075	1.599IleGln: 1.599 ± 0.056	2.559IleArg: 2.559 ± 0.076	5.803IleSer: 5.803 ± 0.107	5.161IleThr: 5.161 ± 0.1	5.723IleVal: 5.723 ± 0.13	0.451IleTrp: 0.451 ± 0.036	2.903IleTyr: 2.903 ± 0.09	0.004IleXaa: 0.004 ± 0.003
Lys	5.496LysAla: 5.496 ± 0.121	1.053LysCys: 1.053 ± 0.046	5.464LysAsp: 5.464 ± 0.106	7.267LysGlu: 7.267 ± 0.143	2.861LysPhe: 2.861 ± 0.078	5.327LysGly: 5.327 ± 0.118	1.098LysHis: 1.098 ± 0.055	6.77LysIle: 6.77 ± 0.138	8.457LysLys: 8.457 ± 0.165	6.642LysLeu: 6.642 ± 0.129	2.557LysMet: 2.557 ± 0.067	5.795LysAsn: 5.795 ± 0.122	2.355LysPro: 2.355 ± 0.076	1.98LysGln: 1.98 ± 0.066	2.947LysArg: 2.947 ± 0.077	5.176LysSer: 5.176 ± 0.11	4.617LysThr: 4.617 ± 0.109	6.46LysVal: 6.46 ± 0.129	0.632LysTrp: 0.632 ± 0.037	4.019LysTyr: 4.019 ± 0.089	0.0LysXaa: 0.0 ± 0.0
Leu	5.26LeuAla: 5.26 ± 0.122	1.517LeuCys: 1.517 ± 0.055	4.721LeuAsp: 4.721 ± 0.104	4.573LeuGlu: 4.573 ± 0.114	3.606LeuPhe: 3.606 ± 0.095	5.384LeuGly: 5.384 ± 0.118	1.236LeuHis: 1.236 ± 0.056	6.14LeuIle: 6.14 ± 0.126	6.981LeuLys: 6.981 ± 0.119	7.253LeuLeu: 7.253 ± 0.18	2.307LeuMet: 2.307 ± 0.07	4.565LeuAsn: 4.565 ± 0.096	3.031LeuPro: 3.031 ± 0.077	2.159LeuGln: 2.159 ± 0.078	2.806LeuArg: 2.806 ± 0.082	6.364LeuSer: 6.364 ± 0.123	4.948LeuThr: 4.948 ± 0.095	5.403LeuVal: 5.403 ± 0.113	0.533LeuTrp: 0.533 ± 0.036	3.078LeuTyr: 3.078 ± 0.08	0.002LeuXaa: 0.002 ± 0.002
Met	2.083MetAla: 2.083 ± 0.064	0.396MetCys: 0.396 ± 0.031	1.633MetAsp: 1.633 ± 0.058	1.822MetGlu: 1.822 ± 0.064	1.127MetPhe: 1.127 ± 0.054	1.913MetGly: 1.913 ± 0.065	0.364MetHis: 0.364 ± 0.026	2.058MetIle: 2.058 ± 0.067	2.54MetLys: 2.54 ± 0.076	2.254MetLeu: 2.254 ± 0.075	0.815MetMet: 0.815 ± 0.041	1.67MetAsn: 1.67 ± 0.06	0.958MetPro: 0.958 ± 0.042	0.636MetGln: 0.636 ± 0.037	0.923MetArg: 0.923 ± 0.042	1.82MetSer: 1.82 ± 0.056	1.544MetThr: 1.544 ± 0.053	1.772MetVal: 1.772 ± 0.057	0.215MetTrp: 0.215 ± 0.017	0.958MetTyr: 0.958 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.358AsnAla: 3.358 ± 0.085	0.96AsnCys: 0.96 ± 0.043	3.425AsnAsp: 3.425 ± 0.089	3.37AsnGlu: 3.37 ± 0.079	2.486AsnPhe: 2.486 ± 0.064	4.476AsnGly: 4.476 ± 0.112	0.963AsnHis: 0.963 ± 0.044	5.163AsnIle: 5.163 ± 0.101	5.317AsnLys: 5.317 ± 0.109	4.598AsnLeu: 4.598 ± 0.107	1.493AsnMet: 1.493 ± 0.045	4.099AsnAsn: 4.099 ± 0.116	2.189AsnPro: 2.189 ± 0.069	1.58AsnGln: 1.58 ± 0.072	1.925AsnArg: 1.925 ± 0.059	4.04AsnSer: 4.04 ± 0.113	3.179AsnThr: 3.179 ± 0.081	4.086AsnVal: 4.086 ± 0.086	0.406AsnTrp: 0.406 ± 0.027	2.696AsnTyr: 2.696 ± 0.084	0.0AsnXaa: 0.0 ± 0.0
Pro	1.704ProAla: 1.704 ± 0.059	0.512ProCys: 0.512 ± 0.033	1.91ProAsp: 1.91 ± 0.06	2.498ProGlu: 2.498 ± 0.077	1.451ProPhe: 1.451 ± 0.053	1.437ProGly: 1.437 ± 0.061	0.508ProHis: 0.508 ± 0.033	2.317ProIle: 2.317 ± 0.069	2.425ProLys: 2.425 ± 0.072	2.465ProLeu: 2.465 ± 0.067	0.784ProMet: 0.784 ± 0.036	1.715ProAsn: 1.715 ± 0.059	0.672ProPro: 0.672 ± 0.03	0.857ProGln: 0.857 ± 0.051	0.813ProArg: 0.813 ± 0.041	1.929ProSer: 1.929 ± 0.071	2.096ProThr: 2.096 ± 0.069	2.345ProVal: 2.345 ± 0.071	0.228ProTrp: 0.228 ± 0.023	1.382ProTyr: 1.382 ± 0.047	0.002ProXaa: 0.002 ± 0.002
Gln	1.47GlnAla: 1.47 ± 0.063	0.327GlnCys: 0.327 ± 0.024	1.178GlnAsp: 1.178 ± 0.051	1.493GlnGlu: 1.493 ± 0.054	0.982GlnPhe: 0.982 ± 0.043	1.567GlnGly: 1.567 ± 0.061	0.411GlnHis: 0.411 ± 0.027	1.961GlnIle: 1.961 ± 0.063	2.079GlnLys: 2.079 ± 0.068	2.123GlnLeu: 2.123 ± 0.062	0.788GlnMet: 0.788 ± 0.04	1.574GlnAsn: 1.574 ± 0.062	0.657GlnPro: 0.657 ± 0.036	0.904GlnGln: 0.904 ± 0.047	0.979GlnArg: 0.979 ± 0.049	1.523GlnSer: 1.523 ± 0.055	1.199GlnThr: 1.199 ± 0.055	1.521GlnVal: 1.521 ± 0.054	0.228GlnTrp: 0.228 ± 0.022	1.262GlnTyr: 1.262 ± 0.054	0.0GlnXaa: 0.0 ± 0.0
Arg	1.742ArgAla: 1.742 ± 0.068	0.466ArgCys: 0.466 ± 0.029	1.814ArgAsp: 1.814 ± 0.063	2.488ArgGlu: 2.488 ± 0.081	1.395ArgPhe: 1.395 ± 0.056	1.967ArgGly: 1.967 ± 0.063	0.524ArgHis: 0.524 ± 0.03	2.81ArgIle: 2.81 ± 0.084	3.099ArgLys: 3.099 ± 0.082	2.625ArgLeu: 2.625 ± 0.074	1.017ArgMet: 1.017 ± 0.046	2.056ArgAsn: 2.056 ± 0.065	0.937ArgPro: 0.937 ± 0.046	1.047ArgGln: 1.047 ± 0.038	1.443ArgArg: 1.443 ± 0.067	1.662ArgSer: 1.662 ± 0.056	1.822ArgThr: 1.822 ± 0.063	2.281ArgVal: 2.281 ± 0.073	0.242ArgTrp: 0.242 ± 0.022	1.359ArgTyr: 1.359 ± 0.056	0.002ArgXaa: 0.002 ± 0.002
Ser	4.226SerAla: 4.226 ± 0.096	0.828SerCys: 0.828 ± 0.04	4.046SerAsp: 4.046 ± 0.099	3.623SerGlu: 3.623 ± 0.088	3.002SerPhe: 3.002 ± 0.079	4.799SerGly: 4.799 ± 0.095	0.958SerHis: 0.958 ± 0.044	4.845SerIle: 4.845 ± 0.105	5.409SerLys: 5.409 ± 0.103	5.675SerLeu: 5.675 ± 0.118	1.672SerMet: 1.672 ± 0.062	3.752SerAsn: 3.752 ± 0.113	1.761SerPro: 1.761 ± 0.057	1.698SerGln: 1.698 ± 0.061	2.138SerArg: 2.138 ± 0.073	4.653SerSer: 4.653 ± 0.159	3.762SerThr: 3.762 ± 0.102	5.167SerVal: 5.167 ± 0.1	0.442SerTrp: 0.442 ± 0.033	2.879SerTyr: 2.879 ± 0.091	0.0SerXaa: 0.0 ± 0.0
Thr	3.893ThrAla: 3.893 ± 0.106	0.763ThrCys: 0.763 ± 0.035	3.802ThrAsp: 3.802 ± 0.088	3.75ThrGlu: 3.75 ± 0.093	2.631ThrPhe: 2.631 ± 0.072	4.325ThrGly: 4.325 ± 0.093	0.975ThrHis: 0.975 ± 0.043	4.655ThrIle: 4.655 ± 0.091	4.327ThrLys: 4.327 ± 0.091	5.296ThrLeu: 5.296 ± 0.094	1.363ThrMet: 1.363 ± 0.049	2.831ThrAsn: 2.831 ± 0.084	2.307ThrPro: 2.307 ± 0.07	1.352ThrGln: 1.352 ± 0.056	1.559ThrArg: 1.559 ± 0.056	3.893ThrSer: 3.893 ± 0.103	4.289ThrThr: 4.289 ± 0.163	5.683ThrVal: 5.683 ± 0.123	0.394ThrTrp: 0.394 ± 0.024	2.591ThrTyr: 2.591 ± 0.1	0.0ThrXaa: 0.0 ± 0.0
Val	5.222ValAla: 5.222 ± 0.113	1.264ValCys: 1.264 ± 0.052	4.512ValAsp: 4.512 ± 0.091	4.232ValGlu: 4.232 ± 0.107	3.12ValPhe: 3.12 ± 0.071	4.525ValGly: 4.525 ± 0.102	0.948ValHis: 0.948 ± 0.043	5.7ValIle: 5.7 ± 0.112	6.001ValLys: 6.001 ± 0.11	6.022ValLeu: 6.022 ± 0.117	1.923ValMet: 1.923 ± 0.057	3.948ValAsn: 3.948 ± 0.09	2.519ValPro: 2.519 ± 0.071	1.536ValGln: 1.536 ± 0.059	2.229ValArg: 2.229 ± 0.069	5.02ValSer: 5.02 ± 0.107	5.095ValThr: 5.095 ± 0.124	5.879ValVal: 5.879 ± 0.125	0.525ValTrp: 0.525 ± 0.035	2.711ValTyr: 2.711 ± 0.067	0.006ValXaa: 0.006 ± 0.004
Trp	0.396TrpAla: 0.396 ± 0.029	0.12TrpCys: 0.12 ± 0.016	0.438TrpAsp: 0.438 ± 0.033	0.36TrpGlu: 0.36 ± 0.023	0.341TrpPhe: 0.341 ± 0.029	0.478TrpGly: 0.478 ± 0.034	0.152TrpHis: 0.152 ± 0.016	0.461TrpIle: 0.461 ± 0.031	0.505TrpLys: 0.505 ± 0.034	0.605TrpLeu: 0.605 ± 0.035	0.177TrpMet: 0.177 ± 0.016	0.392TrpAsn: 0.392 ± 0.025	0.137TrpPro: 0.137 ± 0.019	0.286TrpGln: 0.286 ± 0.022	0.23TrpArg: 0.23 ± 0.019	0.485TrpSer: 0.485 ± 0.034	0.352TrpThr: 0.352 ± 0.031	0.417TrpVal: 0.417 ± 0.033	0.082TrpTrp: 0.082 ± 0.014	0.354TrpTyr: 0.354 ± 0.032	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.212TyrAla: 2.212 ± 0.065	0.805TyrCys: 0.805 ± 0.044	2.974TyrAsp: 2.974 ± 0.075	2.5TyrGlu: 2.5 ± 0.072	2.048TyrPhe: 2.048 ± 0.063	3.067TyrGly: 3.067 ± 0.083	0.668TyrHis: 0.668 ± 0.034	3.37TyrIle: 3.37 ± 0.088	3.575TyrLys: 3.575 ± 0.09	3.208TyrLeu: 3.208 ± 0.076	1.024TyrMet: 1.024 ± 0.045	3.035TyrAsn: 3.035 ± 0.088	1.296TyrPro: 1.296 ± 0.053	1.057TyrGln: 1.057 ± 0.05	1.432TyrArg: 1.432 ± 0.059	3.052TyrSer: 3.052 ± 0.093	2.576TyrThr: 2.576 ± 0.09	2.806TyrVal: 2.806 ± 0.079	0.272TyrTrp: 0.272 ± 0.024	2.296TyrTyr: 2.296 ± 0.084	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.004XaaAla: 0.004 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.008XaaLeu: 0.008 ± 0.004	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.004XaaGln: 0.004 ± 0.003	0.002XaaArg: 0.002 ± 0.002	0.002XaaSer: 0.002 ± 0.002	0.002XaaThr: 0.002 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.015XaaXaa: 0.015 ± 0.007

Statistics based on 1746 proteins (525267 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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