Denitrobacterium detoxificans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Denitrobacterium

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8R313|A0A1H8R313_9ACTN Uncharacterized protein OS=Denitrobacterium detoxificans OX=79604 GN=SAMN02910314_00636 PE=4 SV=1
MM1 pKa = 7.7PSLEE5 pKa = 4.39RR6 pKa = 11.84GMNMICPKK14 pKa = 10.4CGASQLEE21 pKa = 4.37GTKK24 pKa = 10.16FCTSCGSEE32 pKa = 4.39LSGADD37 pKa = 3.03AAQPTVAQPAAPAQPAVAPTQPVAAPTQPVTGTVPPAGYY76 pKa = 6.61TQPGTAPASQPSNGKK91 pKa = 7.95VTGAFVRR98 pKa = 11.84GILSIIFALFMPIVGLILGIVAIAIGSSAYY128 pKa = 10.39RR129 pKa = 11.84EE130 pKa = 4.33TQEE133 pKa = 5.06SRR135 pKa = 11.84AKK137 pKa = 9.44TGKK140 pKa = 7.5TCGIVGLVISVLWIVIAIIFSATIFSATILAMVSSADD177 pKa = 3.55PSLLNSNGGSSINTPATPSTPSSDD201 pKa = 4.11DD202 pKa = 3.06EE203 pKa = 4.37AAAMASVQSYY213 pKa = 11.3LDD215 pKa = 3.64TNLTFSDD222 pKa = 4.32SEE224 pKa = 4.15ISTLASEE231 pKa = 4.55IDD233 pKa = 3.61SSFSSSTGFGLSQIGVDD250 pKa = 3.53PNQFAKK256 pKa = 10.19WLVGDD261 pKa = 4.42LSGTATSATVEE272 pKa = 4.33DD273 pKa = 4.56GEE275 pKa = 4.78GTVTCSITTRR285 pKa = 11.84DD286 pKa = 3.24FDD288 pKa = 4.24YY289 pKa = 11.35FMTDD293 pKa = 3.37FQTSVNNLDD302 pKa = 3.52YY303 pKa = 11.51SSFSDD308 pKa = 3.44MDD310 pKa = 3.6SAYY313 pKa = 10.41QAIGALMLQSMDD325 pKa = 3.28STPVVTRR332 pKa = 11.84TLDD335 pKa = 3.13IDD337 pKa = 3.83VEE339 pKa = 4.45NNLGDD344 pKa = 3.54WDD346 pKa = 3.84IEE348 pKa = 4.49YY349 pKa = 8.5PALTASDD356 pKa = 4.56LSDD359 pKa = 4.94LIYY362 pKa = 11.15GGMNLL367 pKa = 4.49

Molecular weight: 37.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8TPG9|A0A1H8TPG9_9ACTN Phage uncharacterized protein (Phage_XkdX) OS=Denitrobacterium detoxificans OX=79604 GN=SAMN02910314_01651 PE=4 SV=1
MM1 pKa = 7.91KK2 pKa = 10.51SLLLLALGLARR13 pKa = 11.84TVVLGARR20 pKa = 11.84IEE22 pKa = 4.02AEE24 pKa = 3.85RR25 pKa = 11.84IVVSVRR31 pKa = 11.84PYY33 pKa = 9.83KK34 pKa = 10.34RR35 pKa = 11.84EE36 pKa = 3.63QRR38 pKa = 11.84RR39 pKa = 11.84CPVCGRR45 pKa = 11.84ACDD48 pKa = 4.34FYY50 pKa = 12.06DD51 pKa = 3.32MANRR55 pKa = 11.84GAPRR59 pKa = 11.84LWW61 pKa = 4.65

Molecular weight: 6.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1932

0

1932

715596

22

15195

370.4

40.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.151 ± 0.073

1.61 ± 0.044

5.912 ± 0.06

6.403 ± 0.098

3.755 ± 0.04

8.134 ± 0.073

1.889 ± 0.031

5.291 ± 0.069

3.623 ± 0.051

8.647 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.038

3.378 ± 0.073

4.134 ± 0.039

3.201 ± 0.045

5.288 ± 0.103

6.513 ± 0.072

5.897 ± 0.205

7.981 ± 0.053

1.172 ± 0.046

3.295 ± 0.083
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski