Bacteroidales bacterium WCE2004

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1930 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5JNY1|A0A1T5JNY1_9BACT 1-deoxy-D-xylulose 5-phosphate reductoisomerase OS=Bacteroidales bacterium WCE2004 OX=1945890 GN=dxr PE=3 SV=1
MM1 pKa = 7.42MKK3 pKa = 10.18TMDD6 pKa = 5.06HH7 pKa = 6.9IIGMAALALTAATLLACSDD26 pKa = 3.59KK27 pKa = 11.29DD28 pKa = 3.62ALTSAQQPRR37 pKa = 11.84TYY39 pKa = 10.66TLTTTLSPKK48 pKa = 10.5GGVEE52 pKa = 4.3TRR54 pKa = 11.84SSMTDD59 pKa = 2.94NGDD62 pKa = 3.43GSISAAWEE70 pKa = 3.82VGDD73 pKa = 4.7QIWVLYY79 pKa = 8.99TNIGDD84 pKa = 3.84ASEE87 pKa = 4.06EE88 pKa = 4.06TMATVTAVDD97 pKa = 4.46PSTKK101 pKa = 10.35AATISLTLTNPKK113 pKa = 10.21NGGSISFGYY122 pKa = 9.24PAGFFNDD129 pKa = 3.95PKK131 pKa = 10.64DD132 pKa = 3.4PCIAQTGTLDD142 pKa = 5.13DD143 pKa = 4.06INANFAAISGTEE155 pKa = 3.88TMFVFDD161 pKa = 5.6SEE163 pKa = 4.58VTLPDD168 pKa = 3.48VTMMPAMCIWKK179 pKa = 10.12FSFTDD184 pKa = 3.61GTDD187 pKa = 4.24DD188 pKa = 3.19ITSAVTKK195 pKa = 10.28LVVNFPANSMTYY207 pKa = 10.5AVTPTSLDD215 pKa = 3.51NIYY218 pKa = 10.74VAMYY222 pKa = 10.58GDD224 pKa = 5.01IINGQPVSVIAQTDD238 pKa = 3.42LGVYY242 pKa = 9.75RR243 pKa = 11.84KK244 pKa = 7.36TAASVTLASGTTYY257 pKa = 10.66TSTGLEE263 pKa = 4.17LTSAAITNAVADD275 pKa = 5.32DD276 pKa = 3.4IGQLVGADD284 pKa = 3.33GNIYY288 pKa = 10.14PNADD292 pKa = 2.78IATAFGTSGVAMIGYY307 pKa = 9.81VGDD310 pKa = 4.41EE311 pKa = 4.25SDD313 pKa = 3.82CTHH316 pKa = 6.18GVAVALDD323 pKa = 3.98DD324 pKa = 4.44EE325 pKa = 4.55NGKK328 pKa = 10.61AKK330 pKa = 10.02MEE332 pKa = 4.31LSAATAACAAKK343 pKa = 10.49DD344 pKa = 3.8AVPDD348 pKa = 4.07GTWRR352 pKa = 11.84LPSRR356 pKa = 11.84KK357 pKa = 9.04DD358 pKa = 3.06WQYY361 pKa = 11.94LFIGCGSSWTYY372 pKa = 10.25TDD374 pKa = 4.24SPSGSIDD381 pKa = 3.45CSGLQSALDD390 pKa = 3.68AAGGILMSDD399 pKa = 3.88RR400 pKa = 11.84YY401 pKa = 10.03ICEE404 pKa = 3.39EE405 pKa = 3.88GARR408 pKa = 11.84VDD410 pKa = 4.19FTEE413 pKa = 4.69TSASFGFTSNPWYY426 pKa = 10.43VRR428 pKa = 11.84AVLAFF433 pKa = 4.01

Molecular weight: 45.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5ISF5|A0A1T5ISF5_9BACT Threonine/homoserine/homoserine lactone efflux protein OS=Bacteroidales bacterium WCE2004 OX=1945890 GN=SAMN06298214_0510 PE=4 SV=1
MM1 pKa = 7.84PNGKK5 pKa = 8.55KK6 pKa = 9.72HH7 pKa = 6.1KK8 pKa = 7.52RR9 pKa = 11.84HH10 pKa = 5.93KK11 pKa = 9.95MATHH15 pKa = 6.02KK16 pKa = 10.29RR17 pKa = 11.84KK18 pKa = 9.83KK19 pKa = 9.33RR20 pKa = 11.84LRR22 pKa = 11.84LNRR25 pKa = 11.84HH26 pKa = 5.05KK27 pKa = 10.84KK28 pKa = 9.26KK29 pKa = 10.89

Molecular weight: 3.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1930

0

1930

724282

29

1758

375.3

41.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.53 ± 0.061

1.279 ± 0.019

6.062 ± 0.036

6.11 ± 0.046

4.494 ± 0.034

7.923 ± 0.052

1.823 ± 0.021

5.518 ± 0.049

4.835 ± 0.052

9.415 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.024

3.628 ± 0.043

4.403 ± 0.035

3.156 ± 0.025

5.899 ± 0.048

5.777 ± 0.037

5.237 ± 0.035

7.199 ± 0.039

1.41 ± 0.022

3.89 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski