Halovirus VNH-1
Average proteome isoelectric point is 4.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A068IVG8|A0A068IVG8_9VIRU NAD dependent epimerase OS=Halovirus VNH-1 OX=1500510 PE=4 SV=1
MM1 pKa = 7.6 SNEE4 pKa = 3.83 DD5 pKa = 4.62 CEE7 pKa = 4.67 TKK9 pKa = 10.8 DD10 pKa = 3.55 SIEE13 pKa = 4.16 TRR15 pKa = 11.84 TEE17 pKa = 3.79 RR18 pKa = 11.84 ALTEE22 pKa = 4.24 CMTVLPDD29 pKa = 3.39 HH30 pKa = 6.69 GRR32 pKa = 11.84 AEE34 pKa = 4.26 DD35 pKa = 3.82 APGLFVVVGEE45 pKa = 4.36 NCNGEE50 pKa = 4.31 YY51 pKa = 10.67 LVDD54 pKa = 3.85 TRR56 pKa = 11.84 TEE58 pKa = 3.85 SCEE61 pKa = 4.24 CKK63 pKa = 9.81 DD64 pKa = 3.3 AKK66 pKa = 10.95 YY67 pKa = 10.34 RR68 pKa = 11.84 DD69 pKa = 3.79 PEE71 pKa = 4.64 GGCKK75 pKa = 10.06 HH76 pKa = 6.08 IRR78 pKa = 11.84 RR79 pKa = 11.84 CRR81 pKa = 11.84 IAQGEE86 pKa = 4.59 TPVPAGALGEE96 pKa = 4.08 ITIDD100 pKa = 3.57 STFGAQLEE108 pKa = 4.63 TSAKK112 pKa = 9.95 FATADD117 pKa = 3.34 GGIIDD122 pKa = 4.58 AEE124 pKa = 4.32 SGEE127 pKa = 4.44 KK128 pKa = 9.95 ISDD131 pKa = 3.64 EE132 pKa = 4.51 TEE134 pKa = 4.21 STTSWSDD141 pKa = 3.12 PMAEE145 pKa = 3.79 TDD147 pKa = 4.45 KK148 pKa = 11.26 YY149 pKa = 11.08 GKK151 pKa = 8.14 PTGDD155 pKa = 3.65 HH156 pKa = 6.51 YY157 pKa = 10.2 VTCQEE162 pKa = 4.62 CGIEE166 pKa = 4.3 VLTALADD173 pKa = 3.92 CATHH177 pKa = 7.66 RR178 pKa = 11.84 EE179 pKa = 4.57 GCSEE183 pKa = 3.94
Molecular weight: 19.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.251
IPC2_protein 4.355
IPC_protein 4.291
Toseland 4.139
ProMoST 4.368
Dawson 4.24
Bjellqvist 4.418
Wikipedia 4.101
Rodwell 4.126
Grimsley 4.05
Solomon 4.24
Lehninger 4.19
Nozaki 4.342
DTASelect 4.495
Thurlkill 4.139
EMBOSS 4.126
Sillero 4.406
Patrickios 3.121
IPC_peptide 4.24
IPC2_peptide 4.393
IPC2.peptide.svr19 4.314
Protein with the highest isoelectric point:
>tr|A0A068IVW4|A0A068IVW4_9VIRU Hypothetical conserved protein OS=Halovirus VNH-1 OX=1500510 PE=4 SV=1
MM1 pKa = 7.48 SIKK4 pKa = 10.69 EE5 pKa = 4.08 NTLTGAPEE13 pKa = 4.02 TVDD16 pKa = 3.12 EE17 pKa = 4.74 HH18 pKa = 8.83 EE19 pKa = 4.66 EE20 pKa = 3.92 MIQILDD26 pKa = 3.7 EE27 pKa = 5.12 LISEE31 pKa = 4.84 GLRR34 pKa = 11.84 KK35 pKa = 8.64 FTGDD39 pKa = 3.64 GRR41 pKa = 11.84 IRR43 pKa = 11.84 DD44 pKa = 3.78 NKK46 pKa = 9.46 KK47 pKa = 8.95 EE48 pKa = 4.01 KK49 pKa = 10.67 VRR51 pKa = 11.84 IQYY54 pKa = 7.35 MKK56 pKa = 9.78 RR57 pKa = 11.84 TEE59 pKa = 3.68 QAIRR63 pKa = 11.84 AKK65 pKa = 10.35 RR66 pKa = 11.84 QVVKK70 pKa = 10.68 DD71 pKa = 3.61 KK72 pKa = 10.86 QLQQMGRR79 pKa = 11.84 ALEE82 pKa = 4.1 RR83 pKa = 11.84 LQEE86 pKa = 4.23 EE87 pKa = 4.23 NDD89 pKa = 3.4 LDD91 pKa = 4.97 LNLSNN96 pKa = 5.17
Molecular weight: 11.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.804
IPC2_protein 5.83
IPC_protein 5.779
Toseland 5.944
ProMoST 5.944
Dawson 5.842
Bjellqvist 5.893
Wikipedia 5.753
Rodwell 5.792
Grimsley 5.995
Solomon 5.842
Lehninger 5.817
Nozaki 6.008
DTASelect 6.16
Thurlkill 6.008
EMBOSS 5.957
Sillero 6.109
Patrickios 5.156
IPC_peptide 5.855
IPC2_peptide 6.059
IPC2.peptide.svr19 6.352
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1191
75
489
170.1
18.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.137 ± 0.682
1.679 ± 0.961
8.984 ± 1.025
10.243 ± 1.502
1.847 ± 0.52
8.48 ± 1.0
1.511 ± 0.257
5.122 ± 0.727
4.282 ± 0.586
6.969 ± 0.806
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.931 ± 0.594
3.442 ± 0.661
3.023 ± 0.436
4.03 ± 0.864
6.801 ± 0.487
6.969 ± 0.894
7.473 ± 1.553
5.793 ± 0.591
1.259 ± 0.407
3.023 ± 0.569
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here