Pantoea sp. BRM17

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Pantoea; unclassified Pantoea

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2031 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S6W9A1|A0A2S6W9A1_9GAMM Amino acid ABC transporter permease OS=Pantoea sp. BRM17 OX=1343867 GN=CRX72_06050 PE=3 SV=1
MM1 pKa = 7.73DD2 pKa = 3.46QFITEE7 pKa = 4.4VIDD10 pKa = 4.17PNGLAYY16 pKa = 10.43DD17 pKa = 4.12GSGYY21 pKa = 10.34LVWEE25 pKa = 4.84GLVCMQQTGKK35 pKa = 8.69CTDD38 pKa = 3.02EE39 pKa = 4.26HH40 pKa = 7.39RR41 pKa = 11.84EE42 pKa = 4.07LVCKK46 pKa = 9.74WLEE49 pKa = 3.83GRR51 pKa = 11.84EE52 pKa = 4.01LSDD55 pKa = 3.31IQITEE60 pKa = 4.34LFDD63 pKa = 3.16VWSIDD68 pKa = 3.16

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S6VSG3|A0A2S6VSG3_9GAMM Uncharacterized protein OS=Pantoea sp. BRM17 OX=1343867 GN=CRX72_27595 PE=4 SV=1
MM1 pKa = 7.67LSQIQVMPLNSLSVTTQRR19 pKa = 11.84LLKK22 pKa = 9.92RR23 pKa = 11.84TGQRR27 pKa = 11.84LLAFKK32 pKa = 10.17MVFQRR37 pKa = 11.84QANNRR42 pKa = 11.84RR43 pKa = 11.84VAVFKK48 pKa = 10.67IRR50 pKa = 11.84NTVGQFFPANITRR63 pKa = 11.84QRR65 pKa = 11.84FTIIAGKK72 pKa = 10.09

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2031

0

2031

514811

22

2853

253.5

27.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.804 ± 0.076

1.065 ± 0.022

5.07 ± 0.048

5.398 ± 0.065

3.63 ± 0.036

7.484 ± 0.072

2.257 ± 0.036

5.366 ± 0.044

3.629 ± 0.056

11.242 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.03

3.31 ± 0.047

4.574 ± 0.043

4.922 ± 0.055

6.306 ± 0.068

5.905 ± 0.05

5.315 ± 0.068

6.934 ± 0.056

1.506 ± 0.029

2.518 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski