Rhodococcus phage Jace

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Jacevirus; Gordonia virus Jace

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UJ86|A0A2U8UJ86_9CAUD Helix-turn-helix DNA binding protein OS=Rhodococcus phage Jace OX=2182360 GN=38 PE=4 SV=1
MM1 pKa = 7.78LSTLAAIATFVDD13 pKa = 3.97HH14 pKa = 7.12LNEE17 pKa = 4.1PRR19 pKa = 11.84TAALADD25 pKa = 3.45VVAYY29 pKa = 10.16HH30 pKa = 6.6VDD32 pKa = 3.54DD33 pKa = 4.88AGIRR37 pKa = 11.84YY38 pKa = 7.46ATALLADD45 pKa = 4.1GTVTDD50 pKa = 5.4IDD52 pKa = 4.06LQDD55 pKa = 3.15IDD57 pKa = 4.29VRR59 pKa = 11.84PVQPDD64 pKa = 3.79LAAVSS69 pKa = 3.69

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UJ32|A0A2U8UJ32_9CAUD Uncharacterized protein OS=Rhodococcus phage Jace OX=2182360 GN=59 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84LDD4 pKa = 4.47RR5 pKa = 11.84PLQYY9 pKa = 10.55RR10 pKa = 11.84KK11 pKa = 9.82ARR13 pKa = 11.84EE14 pKa = 4.45GIEE17 pKa = 3.88PADD20 pKa = 4.35ALTQNDD26 pKa = 3.96RR27 pKa = 11.84DD28 pKa = 3.97VLITNLLRR36 pKa = 11.84TSRR39 pKa = 11.84DD40 pKa = 3.0VHH42 pKa = 6.18EE43 pKa = 4.5IARR46 pKa = 11.84ITRR49 pKa = 11.84CTPYY53 pKa = 10.29TIDD56 pKa = 4.07RR57 pKa = 11.84ICRR60 pKa = 11.84RR61 pKa = 11.84LRR63 pKa = 11.84RR64 pKa = 11.84EE65 pKa = 3.41HH66 pKa = 7.12AEE68 pKa = 3.55RR69 pKa = 11.84LLTHH73 pKa = 6.16KK74 pKa = 9.19TAAA77 pKa = 3.82

Molecular weight: 9.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

16768

41

1338

180.3

19.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.136 ± 0.453

0.906 ± 0.112

7.019 ± 0.233

5.731 ± 0.229

2.642 ± 0.183

8.242 ± 0.596

2.606 ± 0.249

4.359 ± 0.167

3.519 ± 0.261

8.361 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.115

3.244 ± 0.213

5.087 ± 0.278

3.715 ± 0.219

7.508 ± 0.399

4.986 ± 0.299

6.87 ± 0.234

6.888 ± 0.255

1.747 ± 0.167

2.105 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski