Corynebacterium alimapuense
Average proteome isoelectric point is 5.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2040 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M8K5W8|A0A3M8K5W8_9CORY SAF domain-containing protein OS=Corynebacterium alimapuense OX=1576874 GN=C5L39_08930 PE=4 SV=1
MM1 pKa = 7.93 TYY3 pKa = 10.67 RR4 pKa = 11.84 KK5 pKa = 7.71 TLGVLTATTLALSLVACSDD24 pKa = 3.95 SEE26 pKa = 4.35 GDD28 pKa = 3.85 SSATGDD34 pKa = 3.6 GSDD37 pKa = 3.28 GGNYY41 pKa = 9.4 VLANGSEE48 pKa = 4.18 PQNPLIPANTNEE60 pKa = 4.07 VGGGNIIDD68 pKa = 5.35 LIFSGLVYY76 pKa = 10.61 YY77 pKa = 10.69 DD78 pKa = 3.84 ADD80 pKa = 3.94 GEE82 pKa = 4.38 IHH84 pKa = 6.53 NEE86 pKa = 3.76 VAEE89 pKa = 4.41 SIEE92 pKa = 4.07 LEE94 pKa = 3.81 GDD96 pKa = 2.74 KK97 pKa = 10.45 TYY99 pKa = 10.99 RR100 pKa = 11.84 VVLQDD105 pKa = 2.51 GWTFNDD111 pKa = 4.23 GSAVTADD118 pKa = 3.35 SFVNAWNYY126 pKa = 10.43 AVANSQLSSYY136 pKa = 10.16 FFEE139 pKa = 5.83 PILGYY144 pKa = 10.4 EE145 pKa = 4.21 EE146 pKa = 5.33 GVEE149 pKa = 4.15 SMEE152 pKa = 4.09 GLTVIDD158 pKa = 4.27 DD159 pKa = 3.79 TTFTIEE165 pKa = 4.89 LIEE168 pKa = 4.28 PQADD172 pKa = 3.51 FPTRR176 pKa = 11.84 LGYY179 pKa = 10.24 SAFYY183 pKa = 9.44 PLPEE187 pKa = 4.0 EE188 pKa = 5.25 AFDD191 pKa = 4.62 DD192 pKa = 3.78 MAAFGEE198 pKa = 4.68 TPNGNGPYY206 pKa = 10.2 KK207 pKa = 10.44 LSEE210 pKa = 3.99 WNHH213 pKa = 5.25 NQDD216 pKa = 3.02 ATIVPSEE223 pKa = 4.24 TYY225 pKa = 9.65 EE226 pKa = 4.37 GQRR229 pKa = 11.84 TPQNDD234 pKa = 3.25 GVKK237 pKa = 10.11 FVFYY241 pKa = 10.21 AQQDD245 pKa = 3.29 AAYY248 pKa = 10.25 NDD250 pKa = 4.23 LLAGNLDD257 pKa = 3.9 TLDD260 pKa = 5.46 AIPDD264 pKa = 3.65 SAFATFNEE272 pKa = 4.25 EE273 pKa = 4.02 LGDD276 pKa = 3.74 RR277 pKa = 11.84 AVNQPAAVFQSFTIPMEE294 pKa = 4.22 AEE296 pKa = 3.96 HH297 pKa = 6.8 FSGEE301 pKa = 4.01 EE302 pKa = 3.91 GQLRR306 pKa = 11.84 RR307 pKa = 11.84 QALSLAINRR316 pKa = 11.84 EE317 pKa = 4.34 EE318 pKa = 3.6 ITEE321 pKa = 4.45 TIFQDD326 pKa = 3.14 TRR328 pKa = 11.84 TPASEE333 pKa = 3.89 FTTPVLPGFDD343 pKa = 4.41 DD344 pKa = 5.59 EE345 pKa = 5.08 IPGSEE350 pKa = 4.13 VLDD353 pKa = 4.01 YY354 pKa = 11.25 DD355 pKa = 4.04 PEE357 pKa = 4.34 LANEE361 pKa = 4.63 LWAQADD367 pKa = 4.67 EE368 pKa = 4.19 IDD370 pKa = 3.77 PWSGEE375 pKa = 3.78 FTLSYY380 pKa = 10.58 NADD383 pKa = 3.71 GGHH386 pKa = 6.65 DD387 pKa = 3.11 AWVNAVTNSIKK398 pKa = 9.68 NTLGIEE404 pKa = 4.63 AIGNPYY410 pKa = 10.09 PDD412 pKa = 4.23 FKK414 pKa = 11.17 SLRR417 pKa = 11.84 DD418 pKa = 3.62 DD419 pKa = 3.41 VTNRR423 pKa = 11.84 TIEE426 pKa = 3.98 GAFRR430 pKa = 11.84 TGWQADD436 pKa = 3.95 YY437 pKa = 10.05 PSQGNFLGPLYY448 pKa = 10.0 GTGAGSNDD456 pKa = 3.04 GDD458 pKa = 3.81 YY459 pKa = 11.48 SNPEE463 pKa = 3.42 FDD465 pKa = 5.1 AKK467 pKa = 10.81 LSEE470 pKa = 4.57 ANSASSTEE478 pKa = 3.84 EE479 pKa = 3.28 SSRR482 pKa = 11.84 IFNEE486 pKa = 3.78 SQEE489 pKa = 4.26 ILLSDD494 pKa = 5.0 LPATPLWYY502 pKa = 10.78 SNVAGGFSEE511 pKa = 5.09 NVDD514 pKa = 2.97 NVVFSWKK521 pKa = 10.01 SVPVYY526 pKa = 10.54 YY527 pKa = 10.45 EE528 pKa = 3.38 ITKK531 pKa = 10.19 QQ532 pKa = 3.2
Molecular weight: 57.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.872
Dawson 3.719
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.567
Grimsley 3.439
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.63
Sillero 3.859
Patrickios 1.38
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A3M8K5L2|A0A3M8K5L2_9CORY Non-specific serine/threonine protein kinase OS=Corynebacterium alimapuense OX=1576874 GN=pknB PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.97 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MSTRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.73 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2040
0
2040
679389
33
3023
333.0
36.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.448 ± 0.07
0.653 ± 0.014
6.005 ± 0.047
6.369 ± 0.049
3.245 ± 0.029
8.373 ± 0.047
2.095 ± 0.026
5.368 ± 0.04
2.687 ± 0.041
10.155 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.023
2.697 ± 0.031
4.976 ± 0.037
3.432 ± 0.03
6.164 ± 0.059
6.46 ± 0.043
6.012 ± 0.039
8.162 ± 0.047
1.412 ± 0.026
2.104 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here