Alces alces faeces associated microvirus MP18 4940
Average proteome isoelectric point is 5.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL43|A0A2Z5CL43_9VIRU Nonstructural protein OS=Alces alces faeces associated microvirus MP18 4940 OX=2219137 PE=4 SV=1
MM1 pKa = 7.42 VKK3 pKa = 10.37 FKK5 pKa = 11.07 NWFDD9 pKa = 3.22 DD10 pKa = 3.88 RR11 pKa = 11.84 KK12 pKa = 10.3 RR13 pKa = 11.84 VTSNPGSKK21 pKa = 10.05 FVDD24 pKa = 3.27 VMQPVVQSDD33 pKa = 3.77 GSVEE37 pKa = 3.98 LEE39 pKa = 3.93 VVGKK43 pKa = 10.17 HH44 pKa = 5.38 NLYY47 pKa = 11.01 DD48 pKa = 4.66 EE49 pKa = 4.76 IQSHH53 pKa = 5.94 ARR55 pKa = 11.84 SVDD58 pKa = 2.98 INVILQRR65 pKa = 11.84 YY66 pKa = 8.76 ANGDD70 pKa = 3.58 LSALSARR77 pKa = 11.84 VPMYY81 pKa = 10.96 LDD83 pKa = 3.81 LEE85 pKa = 4.49 GMPTNYY91 pKa = 9.39 MEE93 pKa = 4.44 VLNSVIAGEE102 pKa = 4.69 KK103 pKa = 10.29 FFSDD107 pKa = 3.98 LPADD111 pKa = 3.94 VKK113 pKa = 11.4 SKK115 pKa = 10.84 FDD117 pKa = 3.58 NNFYY121 pKa = 10.95 KK122 pKa = 10.55 FVSSIGSDD130 pKa = 3.25 EE131 pKa = 4.25 FKK133 pKa = 10.97 AAFGLKK139 pKa = 10.08 SSDD142 pKa = 3.25 VVEE145 pKa = 4.3 PVVSVEE151 pKa = 3.95 KK152 pKa = 10.76 EE153 pKa = 4.24 GVISEE158 pKa = 4.31
Molecular weight: 17.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.851
IPC2_protein 4.825
IPC_protein 4.724
Toseland 4.571
ProMoST 4.762
Dawson 4.685
Bjellqvist 4.876
Wikipedia 4.571
Rodwell 4.571
Grimsley 4.482
Solomon 4.685
Lehninger 4.635
Nozaki 4.787
DTASelect 4.978
Thurlkill 4.584
EMBOSS 4.584
Sillero 4.851
Patrickios 4.24
IPC_peptide 4.685
IPC2_peptide 4.838
IPC2.peptide.svr19 4.817
Protein with the highest isoelectric point:
>tr|A0A2Z5CIA3|A0A2Z5CIA3_9VIRU Minor capsid protein OS=Alces alces faeces associated microvirus MP18 4940 OX=2219137 PE=4 SV=1
MM1 pKa = 7.66 PCYY4 pKa = 10.28 HH5 pKa = 7.38 PLKK8 pKa = 10.0 AFKK11 pKa = 10.9 VGITKK16 pKa = 10.38 NNKK19 pKa = 9.4 DD20 pKa = 3.31 KK21 pKa = 11.43 LLITSYY27 pKa = 11.04 KK28 pKa = 9.86 VDD30 pKa = 2.99 HH31 pKa = 6.82 LEE33 pKa = 4.05 YY34 pKa = 10.96 NNRR37 pKa = 11.84 LNKK40 pKa = 9.57 FDD42 pKa = 4.29 RR43 pKa = 11.84 VYY45 pKa = 11.29 SSDD48 pKa = 3.62 LVNPYY53 pKa = 9.89 ADD55 pKa = 3.76 KK56 pKa = 11.15 VISCFDD62 pKa = 3.79 IPCGHH67 pKa = 7.38 CIGCRR72 pKa = 11.84 LDD74 pKa = 3.78 YY75 pKa = 10.78 SRR77 pKa = 11.84 NWACRR82 pKa = 11.84 MLLEE86 pKa = 4.44 VEE88 pKa = 4.38 EE89 pKa = 5.12 HH90 pKa = 6.0 SEE92 pKa = 4.06 SWFCTFTYY100 pKa = 10.84 DD101 pKa = 3.79 NAHH104 pKa = 6.7 IPTSVGYY111 pKa = 10.28 DD112 pKa = 3.16 VDD114 pKa = 4.02 SGEE117 pKa = 4.28 MFPTVTLRR125 pKa = 11.84 KK126 pKa = 9.51 RR127 pKa = 11.84 DD128 pKa = 3.12 WQLFMKK134 pKa = 10.6 RR135 pKa = 11.84 LRR137 pKa = 11.84 KK138 pKa = 9.34 WFDD141 pKa = 3.01 SVYY144 pKa = 10.33 PGEE147 pKa = 4.04 KK148 pKa = 9.65 LKK150 pKa = 11.21 YY151 pKa = 9.2 FAAGEE156 pKa = 4.2 YY157 pKa = 10.72 GDD159 pKa = 3.84 TTFRR163 pKa = 11.84 PHH165 pKa = 4.73 IHH167 pKa = 6.81 AIIFGLHH174 pKa = 6.19 LPDD177 pKa = 5.12 LRR179 pKa = 11.84 PSDD182 pKa = 4.01 RR183 pKa = 11.84 FFSRR187 pKa = 11.84 DD188 pKa = 3.22 FQYY191 pKa = 11.25 YY192 pKa = 9.8 EE193 pKa = 4.28 SDD195 pKa = 3.07 ILSSIWNMGHH205 pKa = 5.47 VVVANVTFEE214 pKa = 4.12 TCAYY218 pKa = 6.21 TARR221 pKa = 11.84 YY222 pKa = 8.27 IVKK225 pKa = 10.23 KK226 pKa = 10.9 LNGKK230 pKa = 8.56 QADD233 pKa = 4.42 LYY235 pKa = 10.44 DD236 pKa = 3.36 QLGIEE241 pKa = 4.5 RR242 pKa = 11.84 EE243 pKa = 3.92 WALMSRR249 pKa = 11.84 KK250 pKa = 9.41 PGIGSAYY257 pKa = 9.12 ADD259 pKa = 3.71 RR260 pKa = 11.84 NIEE263 pKa = 3.94 HH264 pKa = 7.14 LLDD267 pKa = 4.41 DD268 pKa = 4.74 KK269 pKa = 11.58 YY270 pKa = 11.49 LIISTEE276 pKa = 4.0 DD277 pKa = 3.11 GGKK280 pKa = 9.94 KK281 pKa = 10.04 FPVPNYY287 pKa = 9.46 YY288 pKa = 9.89 KK289 pKa = 10.52 RR290 pKa = 11.84 KK291 pKa = 7.75 FDD293 pKa = 3.77 SWFDD297 pKa = 3.57 SQDD300 pKa = 3.33 MVEE303 pKa = 4.22 SSWYY307 pKa = 10.54 KK308 pKa = 10.07 FDD310 pKa = 4.16 RR311 pKa = 11.84 SQAAIRR317 pKa = 11.84 VMEE320 pKa = 4.8 SIKK323 pKa = 10.16 QQQLSNTDD331 pKa = 3.09 LSYY334 pKa = 11.11 IDD336 pKa = 4.6 LLAVNEE342 pKa = 4.23 AAQQAKK348 pKa = 9.58 MKK350 pKa = 10.36 GLEE353 pKa = 4.05 RR354 pKa = 11.84 KK355 pKa = 8.84 VV356 pKa = 3.19
Molecular weight: 41.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.674
IPC2_protein 6.839
IPC_protein 6.883
Toseland 6.781
ProMoST 7.38
Dawson 7.497
Bjellqvist 7.585
Wikipedia 7.439
Rodwell 7.497
Grimsley 6.883
Solomon 7.541
Lehninger 7.556
Nozaki 7.834
DTASelect 7.688
Thurlkill 7.732
EMBOSS 7.775
Sillero 7.936
Patrickios 4.012
IPC_peptide 7.541
IPC2_peptide 7.146
IPC2.peptide.svr19 7.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
897
79
356
224.3
25.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.138 ± 1.988
0.892 ± 0.647
7.135 ± 0.744
5.128 ± 0.616
5.24 ± 0.635
5.351 ± 0.448
1.784 ± 0.552
4.348 ± 0.609
6.689 ± 0.596
6.8 ± 0.665
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.567 ± 0.585
5.797 ± 0.894
2.676 ± 0.69
3.902 ± 0.972
4.125 ± 0.851
10.479 ± 1.607
4.013 ± 0.887
7.692 ± 2.343
1.449 ± 0.449
4.794 ± 0.78
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here