Mycobacterium phage Ochi17

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411BTK5|A0A411BTK5_9CAUD Uncharacterized protein OS=Mycobacterium phage Ochi17 OX=2502425 GN=86 PE=4 SV=1
MM1 pKa = 7.44TCLLCDD7 pKa = 4.01HH8 pKa = 7.18PRR10 pKa = 11.84STHH13 pKa = 5.0TPQCRR18 pKa = 11.84VRR20 pKa = 11.84LGVDD24 pKa = 2.92ADD26 pKa = 3.62DD27 pKa = 3.59MTRR30 pKa = 11.84YY31 pKa = 6.71TQCLCPGFEE40 pKa = 4.22GTEE43 pKa = 4.11EE44 pKa = 5.26EE45 pKa = 4.28EE46 pKa = 4.68DD47 pKa = 3.38TWLDD51 pKa = 3.26

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411BTN2|A0A411BTN2_9CAUD HNH endonuclease OS=Mycobacterium phage Ochi17 OX=2502425 GN=110 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84RR3 pKa = 11.84MLPHH7 pKa = 6.88PRR9 pKa = 11.84PARR12 pKa = 11.84HH13 pKa = 5.82RR14 pKa = 11.84RR15 pKa = 11.84IHH17 pKa = 4.46HH18 pKa = 5.43HH19 pKa = 5.71RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84PTLHH26 pKa = 7.02PPTPRR31 pKa = 11.84PRR33 pKa = 11.84RR34 pKa = 11.84PPSTHH39 pKa = 6.9RR40 pKa = 11.84GTPP43 pKa = 3.19

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

18686

25

1252

169.9

18.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.773 ± 0.485

1.135 ± 0.149

6.486 ± 0.215

6.042 ± 0.291

3.029 ± 0.195

8.621 ± 0.486

2.221 ± 0.182

4.297 ± 0.184

3.388 ± 0.204

7.289 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.119

3.2 ± 0.149

6.004 ± 0.208

3.537 ± 0.204

6.989 ± 0.397

6.24 ± 0.248

6.588 ± 0.266

7.144 ± 0.237

2.339 ± 0.16

2.467 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski