Amino acid dipepetide frequency for Filobacillus milosensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.249AlaAla: 4.249 ± 0.089	0.498AlaCys: 0.498 ± 0.026	3.03AlaAsp: 3.03 ± 0.062	4.124AlaGlu: 4.124 ± 0.078	2.992AlaPhe: 2.992 ± 0.06	4.355AlaGly: 4.355 ± 0.084	1.174AlaHis: 1.174 ± 0.04	5.436AlaIle: 5.436 ± 0.103	4.208AlaLys: 4.208 ± 0.08	6.428AlaLeu: 6.428 ± 0.085	1.754AlaMet: 1.754 ± 0.046	2.693AlaAsn: 2.693 ± 0.053	1.739AlaPro: 1.739 ± 0.045	2.07AlaGln: 2.07 ± 0.047	2.245AlaArg: 2.245 ± 0.054	3.676AlaSer: 3.676 ± 0.074	3.179AlaThr: 3.179 ± 0.063	4.545AlaVal: 4.545 ± 0.074	0.545AlaTrp: 0.545 ± 0.023	2.156AlaTyr: 2.156 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.316CysAla: 0.316 ± 0.021	0.098CysCys: 0.098 ± 0.013	0.371CysAsp: 0.371 ± 0.023	0.453CysGlu: 0.453 ± 0.024	0.276CysPhe: 0.276 ± 0.018	0.599CysGly: 0.599 ± 0.029	0.195CysHis: 0.195 ± 0.015	0.412CysIle: 0.412 ± 0.023	0.318CysLys: 0.318 ± 0.019	0.568CysLeu: 0.568 ± 0.024	0.166CysMet: 0.166 ± 0.013	0.249CysAsn: 0.249 ± 0.017	0.322CysPro: 0.322 ± 0.02	0.205CysGln: 0.205 ± 0.014	0.243CysArg: 0.243 ± 0.018	0.39CysSer: 0.39 ± 0.02	0.328CysThr: 0.328 ± 0.018	0.383CysVal: 0.383 ± 0.02	0.062CysTrp: 0.062 ± 0.008	0.233CysTyr: 0.233 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.153AspAla: 3.153 ± 0.065	0.389AspCys: 0.389 ± 0.024	3.178AspAsp: 3.178 ± 0.074	5.53AspGlu: 5.53 ± 0.095	2.604AspPhe: 2.604 ± 0.052	3.551AspGly: 3.551 ± 0.075	1.38AspHis: 1.38 ± 0.042	4.546AspIle: 4.546 ± 0.074	2.998AspLys: 2.998 ± 0.059	5.627AspLeu: 5.627 ± 0.083	1.447AspMet: 1.447 ± 0.041	2.068AspAsn: 2.068 ± 0.054	2.018AspPro: 2.018 ± 0.049	2.974AspGln: 2.974 ± 0.059	2.34AspArg: 2.34 ± 0.047	2.963AspSer: 2.963 ± 0.058	2.578AspThr: 2.578 ± 0.055	4.263AspVal: 4.263 ± 0.074	0.722AspTrp: 0.722 ± 0.03	2.592AspTyr: 2.592 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	5.154GluAla: 5.154 ± 0.086	0.366GluCys: 0.366 ± 0.021	4.727GluAsp: 4.727 ± 0.082	7.869GluGlu: 7.869 ± 0.13	2.893GluPhe: 2.893 ± 0.061	4.569GluGly: 4.569 ± 0.078	1.747GluHis: 1.747 ± 0.039	5.657GluIle: 5.657 ± 0.092	6.296GluLys: 6.296 ± 0.091	7.541GluLeu: 7.541 ± 0.097	2.22GluMet: 2.22 ± 0.046	4.145GluAsn: 4.145 ± 0.062	2.079GluPro: 2.079 ± 0.046	3.78GluGln: 3.78 ± 0.071	3.434GluArg: 3.434 ± 0.07	3.932GluSer: 3.932 ± 0.067	3.781GluThr: 3.781 ± 0.069	5.614GluVal: 5.614 ± 0.087	0.949GluTrp: 0.949 ± 0.033	2.55GluTyr: 2.55 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.704PheAla: 2.704 ± 0.058	0.284PheCys: 0.284 ± 0.017	2.652PheAsp: 2.652 ± 0.054	3.192PheGlu: 3.192 ± 0.062	2.312PhePhe: 2.312 ± 0.071	3.164PheGly: 3.164 ± 0.068	0.958PheHis: 0.958 ± 0.037	4.233PheIle: 4.233 ± 0.077	2.815PheLys: 2.815 ± 0.051	4.347PheLeu: 4.347 ± 0.092	1.194PheMet: 1.194 ± 0.033	2.38PheAsn: 2.38 ± 0.055	1.584PhePro: 1.584 ± 0.048	1.56PheGln: 1.56 ± 0.041	1.491PheArg: 1.491 ± 0.044	3.146PheSer: 3.146 ± 0.062	2.524PheThr: 2.524 ± 0.059	3.149PheVal: 3.149 ± 0.063	0.478PheTrp: 0.478 ± 0.025	1.909PheTyr: 1.909 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.323GlyAla: 4.323 ± 0.078	0.536GlyCys: 0.536 ± 0.027	3.419GlyAsp: 3.419 ± 0.07	4.745GlyGlu: 4.745 ± 0.082	3.357GlyPhe: 3.357 ± 0.069	4.437GlyGly: 4.437 ± 0.084	1.448GlyHis: 1.448 ± 0.044	5.287GlyIle: 5.287 ± 0.085	4.472GlyLys: 4.472 ± 0.083	6.192GlyLeu: 6.192 ± 0.092	2.017GlyMet: 2.017 ± 0.053	2.629GlyAsn: 2.629 ± 0.058	1.68GlyPro: 1.68 ± 0.046	2.439GlyGln: 2.439 ± 0.057	2.415GlyArg: 2.415 ± 0.055	3.806GlySer: 3.806 ± 0.068	3.529GlyThr: 3.529 ± 0.062	4.991GlyVal: 4.991 ± 0.074	0.704GlyTrp: 0.704 ± 0.027	2.904GlyTyr: 2.904 ± 0.067	0.0GlyXaa: 0.0 ± 0.0
His	1.223HisAla: 1.223 ± 0.037	0.164HisCys: 0.164 ± 0.015	1.203HisAsp: 1.203 ± 0.036	1.596HisGlu: 1.596 ± 0.039	1.052HisPhe: 1.052 ± 0.034	1.356HisGly: 1.356 ± 0.041	0.675HisHis: 0.675 ± 0.03	1.694HisIle: 1.694 ± 0.045	1.16HisLys: 1.16 ± 0.036	2.179HisLeu: 2.179 ± 0.055	0.553HisMet: 0.553 ± 0.023	0.902HisAsn: 0.902 ± 0.031	1.037HisPro: 1.037 ± 0.04	1.033HisGln: 1.033 ± 0.036	0.882HisArg: 0.882 ± 0.031	1.345HisSer: 1.345 ± 0.037	1.019HisThr: 1.019 ± 0.034	1.421HisVal: 1.421 ± 0.04	0.237HisTrp: 0.237 ± 0.016	0.856HisTyr: 0.856 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.348IleAla: 5.348 ± 0.096	0.524IleCys: 0.524 ± 0.022	4.81IleAsp: 4.81 ± 0.076	6.09IleGlu: 6.09 ± 0.086	3.59IlePhe: 3.59 ± 0.069	5.846IleGly: 5.846 ± 0.082	1.672IleHis: 1.672 ± 0.047	6.504IleIle: 6.504 ± 0.109	5.071IleLys: 5.071 ± 0.08	7.208IleLeu: 7.208 ± 0.116	1.883IleMet: 1.883 ± 0.054	4.222IleAsn: 4.222 ± 0.081	3.22IlePro: 3.22 ± 0.063	2.95IleGln: 2.95 ± 0.057	2.907IleArg: 2.907 ± 0.061	5.091IleSer: 5.091 ± 0.091	4.203IleThr: 4.203 ± 0.064	5.529IleVal: 5.529 ± 0.08	0.651IleTrp: 0.651 ± 0.029	2.689IleTyr: 2.689 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	3.986LysAla: 3.986 ± 0.079	0.304LysCys: 0.304 ± 0.019	4.22LysAsp: 4.22 ± 0.065	6.693LysGlu: 6.693 ± 0.083	2.187LysPhe: 2.187 ± 0.05	4.25LysGly: 4.25 ± 0.079	1.557LysHis: 1.557 ± 0.038	4.444LysIle: 4.444 ± 0.065	5.546LysLys: 5.546 ± 0.098	5.75LysLeu: 5.75 ± 0.088	2.004LysMet: 2.004 ± 0.049	3.574LysAsn: 3.574 ± 0.08	2.105LysPro: 2.105 ± 0.048	3.345LysGln: 3.345 ± 0.067	3.222LysArg: 3.222 ± 0.063	3.571LysSer: 3.571 ± 0.057	3.336LysThr: 3.336 ± 0.057	4.724LysVal: 4.724 ± 0.076	0.797LysTrp: 0.797 ± 0.03	2.349LysTyr: 2.349 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	6.234LeuAla: 6.234 ± 0.089	0.5LeuCys: 0.5 ± 0.023	5.313LeuAsp: 5.313 ± 0.076	7.028LeuGlu: 7.028 ± 0.113	4.697LeuPhe: 4.697 ± 0.085	6.173LeuGly: 6.173 ± 0.097	1.736LeuHis: 1.736 ± 0.043	8.099LeuIle: 8.099 ± 0.116	6.74LeuLys: 6.74 ± 0.092	9.299LeuLeu: 9.299 ± 0.135	2.536LeuMet: 2.536 ± 0.052	5.243LeuAsn: 5.243 ± 0.093	3.543LeuPro: 3.543 ± 0.07	3.119LeuGln: 3.119 ± 0.056	3.303LeuArg: 3.303 ± 0.062	6.563LeuSer: 6.563 ± 0.088	5.466LeuThr: 5.466 ± 0.088	6.222LeuVal: 6.222 ± 0.092	0.763LeuTrp: 0.763 ± 0.03	3.221LeuTyr: 3.221 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.8MetAla: 1.8 ± 0.04	0.12MetCys: 0.12 ± 0.01	1.544MetAsp: 1.544 ± 0.037	1.811MetGlu: 1.811 ± 0.044	1.222MetPhe: 1.222 ± 0.038	1.729MetGly: 1.729 ± 0.052	0.408MetHis: 0.408 ± 0.021	2.088MetIle: 2.088 ± 0.052	2.367MetLys: 2.367 ± 0.047	2.513MetLeu: 2.513 ± 0.052	0.904MetMet: 0.904 ± 0.034	1.727MetAsn: 1.727 ± 0.044	0.897MetPro: 0.897 ± 0.032	0.91MetGln: 0.91 ± 0.032	1.061MetArg: 1.061 ± 0.037	1.767MetSer: 1.767 ± 0.047	1.655MetThr: 1.655 ± 0.04	1.92MetVal: 1.92 ± 0.047	0.214MetTrp: 0.214 ± 0.016	0.766MetTyr: 0.766 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	2.509AsnAla: 2.509 ± 0.049	0.301AsnCys: 0.301 ± 0.019	2.994AsnAsp: 2.994 ± 0.06	4.37AsnGlu: 4.37 ± 0.079	1.922AsnPhe: 1.922 ± 0.048	3.108AsnGly: 3.108 ± 0.06	1.374AsnHis: 1.374 ± 0.039	3.728AsnIle: 3.728 ± 0.062	3.451AsnLys: 3.451 ± 0.066	4.214AsnLeu: 4.214 ± 0.072	1.345AsnMet: 1.345 ± 0.036	2.575AsnAsn: 2.575 ± 0.062	2.093AsnPro: 2.093 ± 0.047	2.924AsnGln: 2.924 ± 0.064	2.197AsnArg: 2.197 ± 0.044	2.468AsnSer: 2.468 ± 0.058	2.308AsnThr: 2.308 ± 0.049	3.26AsnVal: 3.26 ± 0.069	0.595AsnTrp: 0.595 ± 0.028	2.019AsnTyr: 2.019 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	1.699ProAla: 1.699 ± 0.045	0.204ProCys: 0.204 ± 0.016	2.033ProAsp: 2.033 ± 0.045	2.866ProGlu: 2.866 ± 0.066	1.854ProPhe: 1.854 ± 0.045	2.08ProGly: 2.08 ± 0.056	0.831ProHis: 0.831 ± 0.032	2.849ProIle: 2.849 ± 0.061	2.188ProLys: 2.188 ± 0.053	3.165ProLeu: 3.165 ± 0.057	0.83ProMet: 0.83 ± 0.032	2.01ProAsn: 2.01 ± 0.052	0.923ProPro: 0.923 ± 0.034	1.069ProGln: 1.069 ± 0.036	1.04ProArg: 1.04 ± 0.032	2.206ProSer: 2.206 ± 0.05	1.884ProThr: 1.884 ± 0.043	2.591ProVal: 2.591 ± 0.051	0.341ProTrp: 0.341 ± 0.019	1.488ProTyr: 1.488 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.677GlnAla: 2.677 ± 0.058	0.154GlnCys: 0.154 ± 0.014	2.036GlnAsp: 2.036 ± 0.054	3.131GlnGlu: 3.131 ± 0.061	1.801GlnPhe: 1.801 ± 0.042	2.359GlnGly: 2.359 ± 0.063	0.82GlnHis: 0.82 ± 0.031	2.984GlnIle: 2.984 ± 0.065	2.768GlnLys: 2.768 ± 0.058	4.396GlnLeu: 4.396 ± 0.076	1.191GlnMet: 1.191 ± 0.036	1.885GlnAsn: 1.885 ± 0.048	1.404GlnPro: 1.404 ± 0.039	1.965GlnGln: 1.965 ± 0.064	1.508GlnArg: 1.508 ± 0.043	2.578GlnSer: 2.578 ± 0.054	2.107GlnThr: 2.107 ± 0.053	2.713GlnVal: 2.713 ± 0.051	0.442GlnTrp: 0.442 ± 0.022	1.485GlnTyr: 1.485 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	2.128ArgAla: 2.128 ± 0.053	0.219ArgCys: 0.219 ± 0.017	2.173ArgAsp: 2.173 ± 0.054	3.18ArgGlu: 3.18 ± 0.064	1.764ArgPhe: 1.764 ± 0.046	2.298ArgGly: 2.298 ± 0.049	0.802ArgHis: 0.802 ± 0.024	2.845ArgIle: 2.845 ± 0.049	2.939ArgLys: 2.939 ± 0.07	3.839ArgLeu: 3.839 ± 0.054	1.168ArgMet: 1.168 ± 0.04	1.805ArgAsn: 1.805 ± 0.04	1.245ArgPro: 1.245 ± 0.04	1.554ArgGln: 1.554 ± 0.036	1.667ArgArg: 1.667 ± 0.046	2.118ArgSer: 2.118 ± 0.048	1.931ArgThr: 1.931 ± 0.048	2.729ArgVal: 2.729 ± 0.056	0.382ArgTrp: 0.382 ± 0.022	1.475ArgTyr: 1.475 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.252SerAla: 3.252 ± 0.071	0.358SerCys: 0.358 ± 0.022	3.33SerAsp: 3.33 ± 0.069	4.347SerGlu: 4.347 ± 0.077	3.198SerPhe: 3.198 ± 0.068	4.075SerGly: 4.075 ± 0.065	1.246SerHis: 1.246 ± 0.039	5.269SerIle: 5.269 ± 0.082	3.946SerLys: 3.946 ± 0.056	5.748SerLeu: 5.748 ± 0.084	1.764SerMet: 1.764 ± 0.05	3.062SerAsn: 3.062 ± 0.062	2.011SerPro: 2.011 ± 0.046	2.26SerGln: 2.26 ± 0.053	2.148SerArg: 2.148 ± 0.049	3.814SerSer: 3.814 ± 0.08	3.022SerThr: 3.022 ± 0.061	4.145SerVal: 4.145 ± 0.08	0.581SerTrp: 0.581 ± 0.024	2.293SerTyr: 2.293 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.098ThrAla: 3.098 ± 0.063	0.329ThrCys: 0.329 ± 0.018	2.818ThrAsp: 2.818 ± 0.065	3.532ThrGlu: 3.532 ± 0.071	2.602ThrPhe: 2.602 ± 0.054	3.662ThrGly: 3.662 ± 0.071	1.076ThrHis: 1.076 ± 0.039	4.62ThrIle: 4.62 ± 0.075	3.091ThrLys: 3.091 ± 0.051	5.157ThrLeu: 5.157 ± 0.072	1.261ThrMet: 1.261 ± 0.032	2.642ThrAsn: 2.642 ± 0.051	2.142ThrPro: 2.142 ± 0.058	1.648ThrGln: 1.648 ± 0.044	1.726ThrArg: 1.726 ± 0.048	3.18ThrSer: 3.18 ± 0.058	2.833ThrThr: 2.833 ± 0.061	3.858ThrVal: 3.858 ± 0.069	0.494ThrTrp: 0.494 ± 0.023	2.057ThrTyr: 2.057 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.61ValAla: 4.61 ± 0.088	0.487ValCys: 0.487 ± 0.023	4.141ValAsp: 4.141 ± 0.071	5.156ValGlu: 5.156 ± 0.089	3.166ValPhe: 3.166 ± 0.064	4.645ValGly: 4.645 ± 0.075	1.336ValHis: 1.336 ± 0.041	5.792ValIle: 5.792 ± 0.098	4.653ValLys: 4.653 ± 0.069	6.739ValLeu: 6.739 ± 0.091	1.931ValMet: 1.931 ± 0.045	3.609ValAsn: 3.609 ± 0.063	2.471ValPro: 2.471 ± 0.051	2.471ValGln: 2.471 ± 0.054	2.498ValArg: 2.498 ± 0.054	4.433ValSer: 4.433 ± 0.074	3.924ValThr: 3.924 ± 0.078	5.141ValVal: 5.141 ± 0.08	0.657ValTrp: 0.657 ± 0.029	2.425ValTyr: 2.425 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.59TrpAla: 0.59 ± 0.027	0.063TrpCys: 0.063 ± 0.008	0.541TrpAsp: 0.541 ± 0.025	0.647TrpGlu: 0.647 ± 0.025	0.577TrpPhe: 0.577 ± 0.028	0.645TrpGly: 0.645 ± 0.027	0.206TrpHis: 0.206 ± 0.016	0.863TrpIle: 0.863 ± 0.032	0.721TrpLys: 0.721 ± 0.03	1.118TrpLeu: 1.118 ± 0.039	0.352TrpMet: 0.352 ± 0.022	0.496TrpAsn: 0.496 ± 0.026	0.243TrpPro: 0.243 ± 0.015	0.374TrpGln: 0.374 ± 0.021	0.377TrpArg: 0.377 ± 0.02	0.585TrpSer: 0.585 ± 0.023	0.491TrpThr: 0.491 ± 0.025	0.752TrpVal: 0.752 ± 0.034	0.144TrpTrp: 0.144 ± 0.013	0.417TrpTyr: 0.417 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.044TyrAla: 2.044 ± 0.057	0.288TyrCys: 0.288 ± 0.018	2.388TyrAsp: 2.388 ± 0.053	2.942TyrGlu: 2.942 ± 0.056	2.011TyrPhe: 2.011 ± 0.045	2.431TyrGly: 2.431 ± 0.052	0.945TyrHis: 0.945 ± 0.035	2.656TyrIle: 2.656 ± 0.058	2.213TyrLys: 2.213 ± 0.06	3.771TyrLeu: 3.771 ± 0.056	0.903TyrMet: 0.903 ± 0.033	1.834TyrAsn: 1.834 ± 0.047	1.399TyrPro: 1.399 ± 0.042	1.751TyrGln: 1.751 ± 0.047	1.559TyrArg: 1.559 ± 0.043	2.238TyrSer: 2.238 ± 0.045	1.728TyrThr: 1.728 ± 0.049	2.335TyrVal: 2.335 ± 0.051	0.446TyrTrp: 0.446 ± 0.022	1.674TyrTyr: 1.674 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3330 proteins (921258 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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