Capybara microvirus Cap1_SP_66
Average proteome isoelectric point is 5.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7J6|A0A4P8W7J6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_66 OX=2584788 PE=4 SV=1
MM1 pKa = 7.42 TYY3 pKa = 10.53 SIIALYY9 pKa = 10.25 EE10 pKa = 4.11 DD11 pKa = 4.41 EE12 pKa = 5.43 NEE14 pKa = 4.31 CLHH17 pKa = 6.96 QITLFNRR24 pKa = 11.84 LCSLDD29 pKa = 3.45 SALPLLKK36 pKa = 10.72 NIFQTPSDD44 pKa = 4.38 YY45 pKa = 10.55 IACDD49 pKa = 3.32 SLVLVDD55 pKa = 6.0 CYY57 pKa = 10.94 IVDD60 pKa = 3.83 CEE62 pKa = 4.25 GFRR65 pKa = 11.84 VRR67 pKa = 11.84 VSKK70 pKa = 11.17 NN71 pKa = 2.82
Molecular weight: 8.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.256
IPC2_protein 4.444
IPC_protein 4.279
Toseland 4.088
ProMoST 4.431
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.19
Rodwell 4.113
Grimsley 4.012
Solomon 4.24
Lehninger 4.202
Nozaki 4.38
DTASelect 4.584
Thurlkill 4.139
EMBOSS 4.19
Sillero 4.393
Patrickios 0.362
IPC_peptide 4.24
IPC2_peptide 4.38
IPC2.peptide.svr19 4.308
Protein with the highest isoelectric point:
>tr|A0A4P8W3Y1|A0A4P8W3Y1_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_66 OX=2584788 PE=3 SV=1
MM1 pKa = 7.27 SCSNPTYY8 pKa = 10.47 FFNKK12 pKa = 9.81 KK13 pKa = 10.17 FIQRR17 pKa = 11.84 YY18 pKa = 7.74 YY19 pKa = 10.03 SLVDD23 pKa = 3.18 GFHH26 pKa = 5.86 YY27 pKa = 10.73 HH28 pKa = 6.43 GVDD31 pKa = 3.52 YY32 pKa = 10.15 PFKK35 pKa = 10.56 PSVSDD40 pKa = 3.36 VASIFDD46 pKa = 3.8 EE47 pKa = 5.25 FKK49 pKa = 10.87 AGQTFDD55 pKa = 3.16 FRR57 pKa = 11.84 LYY59 pKa = 10.01 LTGAYY64 pKa = 10.25 DD65 pKa = 3.51 GDD67 pKa = 4.44 YY68 pKa = 10.59 VVHH71 pKa = 5.96 YY72 pKa = 8.16 PINPFLVIPCHH83 pKa = 5.78 NCPSCLHH90 pKa = 7.05 DD91 pKa = 3.85 KK92 pKa = 10.64 QATKK96 pKa = 10.64 SALIALEE103 pKa = 4.39 SVGKK107 pKa = 9.33 PSPLFVTLTYY117 pKa = 10.82 ADD119 pKa = 3.9 EE120 pKa = 5.31 PINGVSVRR128 pKa = 11.84 DD129 pKa = 3.44 VQLFLKK135 pKa = 10.16 RR136 pKa = 11.84 LRR138 pKa = 11.84 KK139 pKa = 8.74 MFPQSKK145 pKa = 9.36 IRR147 pKa = 11.84 YY148 pKa = 7.82 HH149 pKa = 5.9 CTAEE153 pKa = 4.37 YY154 pKa = 10.68 GSHH157 pKa = 6.22 TYY159 pKa = 9.92 RR160 pKa = 11.84 PHH162 pKa = 4.93 YY163 pKa = 9.87 HH164 pKa = 6.75 LLIFGLPLEE173 pKa = 4.59 SEE175 pKa = 4.4 QFVEE179 pKa = 5.12 SIKK182 pKa = 10.81 SRR184 pKa = 11.84 YY185 pKa = 8.37 NVPNYY190 pKa = 9.3 PVGYY194 pKa = 9.74 GYY196 pKa = 11.25 YY197 pKa = 9.43 LDD199 pKa = 3.92 YY200 pKa = 10.84 SIRR203 pKa = 11.84 QAWQLGITHH212 pKa = 6.19 SRR214 pKa = 11.84 VVLNPAVIRR223 pKa = 11.84 YY224 pKa = 7.26 VSKK227 pKa = 9.85 YY228 pKa = 7.4 TSKK231 pKa = 10.81 QSDD234 pKa = 3.73 VLLGKK239 pKa = 10.02 NPPFMLQSTHH249 pKa = 6.21 NGGLGASVLRR259 pKa = 11.84 DD260 pKa = 3.25 YY261 pKa = 10.96 IINNNFDD268 pKa = 4.29 SNCGISLNGRR278 pKa = 11.84 YY279 pKa = 7.43 FTIPSNLFNQRR290 pKa = 11.84 VCLSINRR297 pKa = 11.84 YY298 pKa = 8.4 IGYY301 pKa = 9.69 YY302 pKa = 8.22 WLLVMKK308 pKa = 10.53 ALDD311 pKa = 4.23 HH312 pKa = 7.26 LSLLPDD318 pKa = 4.27 LPPIIMQAVHH328 pKa = 6.57 SYY330 pKa = 9.89 IVPRR334 pKa = 11.84 GTNKK338 pKa = 10.11 SLEE341 pKa = 3.95 FLEE344 pKa = 4.75 YY345 pKa = 10.48 INDD348 pKa = 3.39 EE349 pKa = 4.43 TFPARR354 pKa = 11.84 YY355 pKa = 8.83 IPAVSARR362 pKa = 11.84 FNDD365 pKa = 4.43 DD366 pKa = 3.18 YY367 pKa = 11.57 AQKK370 pKa = 10.86 LEE372 pKa = 3.83 QFLKK376 pKa = 10.39 IKK378 pKa = 10.67 HH379 pKa = 5.37 EE380 pKa = 5.0 FIAKK384 pKa = 8.46 RR385 pKa = 11.84 TIPEE389 pKa = 3.91 VPNQNFLQNYY399 pKa = 7.78 RR400 pKa = 11.84 AEE402 pKa = 4.16 IQLNRR407 pKa = 11.84 QKK409 pKa = 10.93 EE410 pKa = 4.27 KK411 pKa = 11.23 EE412 pKa = 4.09 KK413 pKa = 11.13 LL414 pKa = 3.49
Molecular weight: 47.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.358
IPC2_protein 8.346
IPC_protein 8.229
Toseland 8.39
ProMoST 8.624
Dawson 8.916
Bjellqvist 8.96
Wikipedia 9.063
Rodwell 8.96
Grimsley 8.741
Solomon 8.99
Lehninger 8.946
Nozaki 8.946
DTASelect 8.829
Thurlkill 8.843
EMBOSS 9.033
Sillero 9.063
Patrickios 3.503
IPC_peptide 8.975
IPC2_peptide 7.849
IPC2.peptide.svr19 7.827
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1777
71
750
296.2
33.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.246 ± 0.693
1.463 ± 0.288
5.909 ± 0.371
4.333 ± 0.746
4.502 ± 1.022
5.459 ± 0.606
2.195 ± 0.493
6.078 ± 0.243
5.065 ± 0.413
9.06 ± 0.844
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.589 ± 0.42
6.64 ± 0.586
5.065 ± 0.597
4.952 ± 0.629
4.389 ± 0.271
7.822 ± 0.532
5.796 ± 0.654
5.965 ± 0.273
0.732 ± 0.193
5.74 ± 0.957
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here