Capybara microvirus Cap1_SP_240
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVR4|A0A4V1FVR4_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_240 OX=2585417 PE=3 SV=1
MM1 pKa = 7.76 IYY3 pKa = 10.19 FVYY6 pKa = 10.44 SIYY9 pKa = 11.07 DD10 pKa = 3.77 SVDD13 pKa = 2.7 KK14 pKa = 11.02 SYY16 pKa = 10.77 TPPQLYY22 pKa = 9.86 RR23 pKa = 11.84 SEE25 pKa = 5.64 DD26 pKa = 3.32 SAQRR30 pKa = 11.84 AFNHH34 pKa = 5.67 FVEE37 pKa = 5.21 CNFIGKK43 pKa = 8.83 DD44 pKa = 3.53 CKK46 pKa = 10.52 LVCLGSWSDD55 pKa = 3.73 TDD57 pKa = 5.4 CKK59 pKa = 10.86 FCLLDD64 pKa = 3.95 PYY66 pKa = 10.46 EE67 pKa = 4.63 VYY69 pKa = 11.06 VSGEE73 pKa = 4.19 VKK75 pKa = 10.63 SDD77 pKa = 3.32 VV78 pKa = 3.38
Molecular weight: 9.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.365
IPC2_protein 4.507
IPC_protein 4.368
Toseland 4.177
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.291
Rodwell 4.202
Grimsley 4.088
Solomon 4.342
Lehninger 4.304
Nozaki 4.469
DTASelect 4.698
Thurlkill 4.228
EMBOSS 4.304
Sillero 4.495
Patrickios 0.35
IPC_peptide 4.342
IPC2_peptide 4.469
IPC2.peptide.svr19 4.397
Protein with the highest isoelectric point:
>tr|A0A4P8W4L3|A0A4P8W4L3_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_240 OX=2585417 PE=4 SV=1
MM1 pKa = 7.95 CINPKK6 pKa = 9.54 RR7 pKa = 11.84 VKK9 pKa = 10.14 IKK11 pKa = 10.69 NPCHH15 pKa = 6.43 FNGALNEE22 pKa = 4.04 FFRR25 pKa = 11.84 EE26 pKa = 4.0 PFIIRR31 pKa = 11.84 YY32 pKa = 7.49 VPCKK36 pKa = 10.33 RR37 pKa = 11.84 CVEE40 pKa = 4.17 CQINEE45 pKa = 4.25 SKK47 pKa = 10.45 EE48 pKa = 3.56 WALRR52 pKa = 11.84 MSLEE56 pKa = 3.75 ADD58 pKa = 3.91 KK59 pKa = 11.62 YY60 pKa = 10.62 QDD62 pKa = 3.63 CCFLTLTYY70 pKa = 11.13 DD71 pKa = 3.8 DD72 pKa = 5.39 DD73 pKa = 4.57 NNPRR77 pKa = 11.84 SLNKK81 pKa = 9.31 TDD83 pKa = 3.36 YY84 pKa = 11.09 QKK86 pKa = 10.99 FLKK89 pKa = 10.22 RR90 pKa = 11.84 LRR92 pKa = 11.84 KK93 pKa = 9.68 HH94 pKa = 6.41 ISPTKK99 pKa = 9.71 IKK101 pKa = 10.26 YY102 pKa = 9.48 FLSAEE107 pKa = 4.21 YY108 pKa = 10.38 GGKK111 pKa = 7.22 TFRR114 pKa = 11.84 PHH116 pKa = 4.25 YY117 pKa = 10.0 HH118 pKa = 6.99 LIIFGWKK125 pKa = 9.81 PEE127 pKa = 4.09 DD128 pKa = 3.76 AFPLKK133 pKa = 10.52 VDD135 pKa = 3.41 EE136 pKa = 5.59 KK137 pKa = 11.29 GNQLYY142 pKa = 10.39 YY143 pKa = 10.86 SPTVAKK149 pKa = 9.56 LWLYY153 pKa = 9.86 GFHH156 pKa = 7.03 SVGEE160 pKa = 4.15 PTQQSMLYY168 pKa = 8.53 CAKK171 pKa = 10.25 YY172 pKa = 9.48 LQKK175 pKa = 10.62 LQSVPDD181 pKa = 3.97 GFVKK185 pKa = 10.54 PFVAMSKK192 pKa = 9.47 MDD194 pKa = 3.67 GPLGYY199 pKa = 9.35 PGQLSEE205 pKa = 4.51 GQSTYY210 pKa = 11.44 DD211 pKa = 3.17 RR212 pKa = 11.84 LSYY215 pKa = 10.31 AGKK218 pKa = 9.84 IEE220 pKa = 4.04 YY221 pKa = 9.96 VPQSFWRR228 pKa = 11.84 RR229 pKa = 11.84 AVKK232 pKa = 10.32 DD233 pKa = 3.66 DD234 pKa = 3.45 EE235 pKa = 5.41 LIRR238 pKa = 11.84 LDD240 pKa = 3.16 RR241 pKa = 11.84 ALAVEE246 pKa = 3.95 RR247 pKa = 11.84 RR248 pKa = 11.84 LRR250 pKa = 11.84 SFKK253 pKa = 10.78 IPDD256 pKa = 3.52 LEE258 pKa = 4.24 SVEE261 pKa = 4.1 RR262 pKa = 11.84 RR263 pKa = 11.84 KK264 pKa = 10.21 QRR266 pKa = 11.84 ILEE269 pKa = 4.09 NLHH272 pKa = 7.02 LGLDD276 pKa = 3.5 KK277 pKa = 11.07 QKK279 pKa = 11.24 NIDD282 pKa = 3.54 YY283 pKa = 11.13 NKK285 pKa = 9.96 IEE287 pKa = 4.38 VKK289 pKa = 9.64 RR290 pKa = 11.84 AHH292 pKa = 5.26 VRR294 pKa = 11.84 RR295 pKa = 11.84 VNARR299 pKa = 11.84 MRR301 pKa = 11.84 VRR303 pKa = 11.84 TRR305 pKa = 11.84 KK306 pKa = 10.2 AMIDD310 pKa = 3.5 YY311 pKa = 8.74 QLSMRR316 pKa = 11.84 EE317 pKa = 3.86 VYY319 pKa = 10.34 DD320 pKa = 3.37 YY321 pKa = 11.8 DD322 pKa = 5.18 KK323 pKa = 11.27 IFF325 pKa = 3.77
Molecular weight: 38.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.861
IPC2_protein 8.946
IPC_protein 8.843
Toseland 9.458
ProMoST 9.282
Dawson 9.765
Bjellqvist 9.516
Wikipedia 9.955
Rodwell 10.014
Grimsley 9.853
Solomon 9.794
Lehninger 9.736
Nozaki 9.604
DTASelect 9.472
Thurlkill 9.604
EMBOSS 9.911
Sillero 9.721
Patrickios 4.8
IPC_peptide 9.78
IPC2_peptide 8.244
IPC2.peptide.svr19 7.853
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1360
78
552
226.7
25.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.618 ± 1.305
1.397 ± 0.632
5.882 ± 0.483
5.735 ± 0.806
4.779 ± 0.957
5.588 ± 0.928
1.691 ± 0.272
5.0 ± 0.565
6.397 ± 1.155
7.941 ± 0.549
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.985 ± 0.258
5.809 ± 0.657
4.706 ± 0.626
4.265 ± 0.564
5.368 ± 0.849
7.426 ± 0.682
5.662 ± 0.882
6.912 ± 0.53
1.25 ± 0.26
5.588 ± 0.933
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here