Fundicoccus ignavus
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2258 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I2GPF8|A0A6I2GPF8_9LACT Uncharacterized protein OS=Fundicoccus ignavus OX=2664442 GN=GIY09_05520 PE=4 SV=1
MM1 pKa = 7.82 KK2 pKa = 10.24 KK3 pKa = 10.26 RR4 pKa = 11.84 LILTGLTLLALGGILPSTHH23 pKa = 5.64 SVYY26 pKa = 11.0 AEE28 pKa = 3.87 EE29 pKa = 4.55 LVSDD33 pKa = 3.58 VTLNVGILPAEE44 pKa = 3.99 SAIPIIIAKK53 pKa = 9.37 EE54 pKa = 3.74 NGYY57 pKa = 10.44 FEE59 pKa = 5.81 ALGLDD64 pKa = 3.75 VDD66 pKa = 3.96 IHH68 pKa = 5.84 TFAAPTDD75 pKa = 3.81 RR76 pKa = 11.84 NIAIQAKK83 pKa = 9.65 EE84 pKa = 4.08 LDD86 pKa = 3.49 ATIADD91 pKa = 3.97 VMTEE95 pKa = 3.83 ATFKK99 pKa = 11.15 QNGIDD104 pKa = 3.63 MVITSDD110 pKa = 3.1 ILEE113 pKa = 4.52 DD114 pKa = 4.16 FKK116 pKa = 11.92 VLASPNSGVTEE127 pKa = 4.05 MEE129 pKa = 3.83 EE130 pKa = 4.86 LADD133 pKa = 4.45 KK134 pKa = 10.98 DD135 pKa = 3.32 ITLVPNFILEE145 pKa = 4.43 YY146 pKa = 10.72 IMDD149 pKa = 4.24 VYY151 pKa = 10.61 ATEE154 pKa = 5.2 GNFTYY159 pKa = 10.72 NIVEE163 pKa = 4.03 ITSFSARR170 pKa = 11.84 SEE172 pKa = 4.02 ALLNDD177 pKa = 3.55 QVDD180 pKa = 3.5 AAVYY184 pKa = 8.47 TEE186 pKa = 4.6 PQASMLVAQGAINLGSSADD205 pKa = 3.58 AGIKK209 pKa = 10.43 GGTIQFMADD218 pKa = 3.04 VVAEE222 pKa = 4.34 RR223 pKa = 11.84 PQDD226 pKa = 3.07 ISAFYY231 pKa = 9.8 QAYY234 pKa = 9.16 NQAIDD239 pKa = 3.66 YY240 pKa = 9.25 MNEE243 pKa = 3.45 NSAASYY249 pKa = 11.57 AEE251 pKa = 3.75 VLSNYY256 pKa = 7.31 EE257 pKa = 3.91 FPEE260 pKa = 4.25 AMSTYY265 pKa = 10.49 LDD267 pKa = 4.07 SKK269 pKa = 10.4 EE270 pKa = 3.85 VAYY273 pKa = 9.05 PYY275 pKa = 10.72 AQAVPQDD282 pKa = 3.41 QFDD285 pKa = 4.98 NIIEE289 pKa = 4.22 WTVSKK294 pKa = 10.8 GQIDD298 pKa = 3.46 TAYY301 pKa = 9.72 TYY303 pKa = 11.25 DD304 pKa = 3.65 EE305 pKa = 4.3 LTNFTFLTEE314 pKa = 3.94
Molecular weight: 34.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.63
ProMoST 3.948
Dawson 3.795
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.948
Patrickios 1.214
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A6I2GNS9|A0A6I2GNS9_9LACT CBS domain-containing protein OS=Fundicoccus ignavus OX=2664442 GN=GIY09_09810 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.36 KK9 pKa = 7.48 RR10 pKa = 11.84 TRR12 pKa = 11.84 KK13 pKa = 9.06 KK14 pKa = 8.41 VHH16 pKa = 5.85 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.02 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.59 GRR39 pKa = 11.84 KK40 pKa = 8.94 KK41 pKa = 9.83 IAAA44 pKa = 3.97
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.416
IPC2_protein 11.111
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.179
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.995
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2258
0
2258
718277
14
3562
318.1
35.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.709 ± 0.053
0.484 ± 0.012
5.576 ± 0.039
7.601 ± 0.06
4.39 ± 0.041
6.386 ± 0.047
1.976 ± 0.026
7.661 ± 0.051
5.416 ± 0.044
9.986 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.618 ± 0.023
4.793 ± 0.038
3.404 ± 0.028
4.297 ± 0.042
3.948 ± 0.038
6.119 ± 0.04
5.8 ± 0.045
7.13 ± 0.044
0.913 ± 0.017
3.794 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here