Bombella sp. KACC 21507
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1570 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P1NIE7|A0A6P1NIE7_9PROT Uncharacterized protein OS=Bombella sp. KACC 21507 OX=2697033 GN=GT348_08845 PE=4 SV=1
MM1 pKa = 7.37 TYY3 pKa = 10.71 VVTEE7 pKa = 3.51 NCIRR11 pKa = 11.84 CKK13 pKa = 11.02 YY14 pKa = 7.77 MDD16 pKa = 4.38 CVEE19 pKa = 4.32 VCPVDD24 pKa = 3.86 CFYY27 pKa = 11.41 AGEE30 pKa = 4.14 NFLVISPDD38 pKa = 3.09 EE39 pKa = 4.97 CIDD42 pKa = 3.9 CGVCEE47 pKa = 4.44 PEE49 pKa = 4.91 CPAEE53 pKa = 4.95 AIFPDD58 pKa = 3.51 SDD60 pKa = 3.59 EE61 pKa = 5.44 RR62 pKa = 11.84 SGPWMEE68 pKa = 4.4 TNAKK72 pKa = 8.73 FAKK75 pKa = 9.31 VWPNLSQKK83 pKa = 8.55 GTAPADD89 pKa = 3.41 AEE91 pKa = 4.22 EE92 pKa = 4.47 WNGKK96 pKa = 7.35 SDD98 pKa = 3.26 KK99 pKa = 10.93 AQYY102 pKa = 10.45 LSEE105 pKa = 5.27 APHH108 pKa = 6.05 QDD110 pKa = 2.76
Molecular weight: 12.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.97
IPC2_protein 4.139
IPC_protein 4.05
Toseland 3.872
ProMoST 4.113
Dawson 4.012
Bjellqvist 4.202
Wikipedia 3.897
Rodwell 3.884
Grimsley 3.783
Solomon 3.999
Lehninger 3.948
Nozaki 4.126
DTASelect 4.279
Thurlkill 3.897
EMBOSS 3.91
Sillero 4.164
Patrickios 0.401
IPC_peptide 3.999
IPC2_peptide 4.151
IPC2.peptide.svr19 4.096
Protein with the highest isoelectric point:
>tr|A0A6P1NCY5|A0A6P1NCY5_9PROT Uncharacterized protein OS=Bombella sp. KACC 21507 OX=2697033 GN=GT348_03025 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.11 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 SATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 TKK37 pKa = 10.41 GRR39 pKa = 11.84 KK40 pKa = 8.93 KK41 pKa = 10.59 LSAA44 pKa = 3.95
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1570
0
1570
501926
37
2861
319.7
35.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.304 ± 0.063
1.02 ± 0.021
5.168 ± 0.041
5.881 ± 0.07
3.927 ± 0.045
7.395 ± 0.073
2.528 ± 0.03
6.393 ± 0.051
5.247 ± 0.065
10.746 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.247 ± 0.028
3.778 ± 0.062
4.703 ± 0.044
3.869 ± 0.041
5.71 ± 0.061
6.784 ± 0.063
5.296 ± 0.049
6.208 ± 0.062
1.261 ± 0.026
2.533 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here