Staphylococcus phage PVL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; Staphylococcus virus PVL

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O80096|O80096_9CAUD Uncharacterized protein OS=Staphylococcus phage PVL OX=71366 PE=4 SV=1
MM1 pKa = 7.28GCLVVVKK8 pKa = 10.07EE9 pKa = 3.89ILRR12 pKa = 11.84LLFLLAMYY20 pKa = 10.04EE21 pKa = 3.83LGKK24 pKa = 10.83YY25 pKa = 7.27VTEE28 pKa = 3.84QVYY31 pKa = 10.94IMMTANDD38 pKa = 4.14DD39 pKa = 3.7VEE41 pKa = 5.3APSDD45 pKa = 3.63YY46 pKa = 11.0VFRR49 pKa = 11.84AEE51 pKa = 4.19VSEE54 pKa = 4.17

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O80066|O80066_9CAUD LukS-PV OS=Staphylococcus phage PVL OX=71366 PE=1 SV=1
MM1 pKa = 7.54IKK3 pKa = 10.33KK4 pKa = 9.52LLWSTISWFYY14 pKa = 11.55VYY16 pKa = 10.47YY17 pKa = 9.12QSKK20 pKa = 9.42YY21 pKa = 10.26KK22 pKa = 10.83LFIICLVMNGFFRR35 pKa = 11.84NNSKK39 pKa = 8.53KK40 pKa = 8.2THH42 pKa = 5.55LL43 pKa = 4.1

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12383

43

759

199.7

22.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.176 ± 0.477

0.557 ± 0.102

6.178 ± 0.276

7.26 ± 0.476

3.973 ± 0.235

5.701 ± 0.466

1.712 ± 0.181

7.89 ± 0.284

9.117 ± 0.332

8.092 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.188

7.155 ± 0.28

2.512 ± 0.178

4.03 ± 0.248

4.329 ± 0.234

6.202 ± 0.273

6.162 ± 0.239

5.903 ± 0.23

1.09 ± 0.143

4.361 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski