uncultured marine virus

Taxonomy: Viruses; environmental samples

Average proteome isoelectric point is 8.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9MHA2|M9MHA2_9VIRU Replication protein OS=uncultured marine virus OX=186617 PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84SQHH5 pKa = 5.85QFSQVPRR12 pKa = 11.84ADD14 pKa = 3.13IARR17 pKa = 11.84SSFNLSHH24 pKa = 6.2GVKK27 pKa = 9.11STFDD31 pKa = 3.24VDD33 pKa = 3.96FLVPILVMDD42 pKa = 5.74IIPGDD47 pKa = 3.8TFNVKK52 pKa = 10.24CSFFMRR58 pKa = 11.84LATPLHH64 pKa = 6.26PLMDD68 pKa = 3.62NMYY71 pKa = 10.08VEE73 pKa = 4.53TFFFFVAYY81 pKa = 8.37RR82 pKa = 11.84TIWDD86 pKa = 3.79SFEE89 pKa = 4.34KK90 pKa = 10.64FHH92 pKa = 7.04GAQDD96 pKa = 3.99DD97 pKa = 4.17PGDD100 pKa = 4.66SIDD103 pKa = 3.59FTIPVLSAGIVGTGDD118 pKa = 3.0LSDD121 pKa = 4.09YY122 pKa = 10.47MGLPLALDD130 pKa = 3.6ASVTNDD136 pKa = 2.97VSSLPFRR143 pKa = 11.84AYY145 pKa = 10.48RR146 pKa = 11.84KK147 pKa = 9.04IWNDD151 pKa = 2.61WFRR154 pKa = 11.84DD155 pKa = 3.47EE156 pKa = 5.31NLQDD160 pKa = 3.51TLDD163 pKa = 3.9FNTNNGPDD171 pKa = 3.48NNTNSGMADD180 pKa = 2.96IPLKK184 pKa = 10.01RR185 pKa = 11.84GKK187 pKa = 10.37RR188 pKa = 11.84FDD190 pKa = 4.01YY191 pKa = 8.25FTSSLPAPQKK201 pKa = 10.56GSAVALPLGTRR212 pKa = 11.84ASIATDD218 pKa = 3.42AAAGGLITVKK228 pKa = 10.43ALHH231 pKa = 6.73PALLTQNQLDD241 pKa = 3.8AGGATVDD248 pKa = 4.42LSATTGATGDD258 pKa = 3.95EE259 pKa = 4.44LFADD263 pKa = 4.69LTNATAATINDD274 pKa = 3.0IRR276 pKa = 11.84LAFQTQKK283 pKa = 10.97LIEE286 pKa = 4.58RR287 pKa = 11.84DD288 pKa = 3.39ARR290 pKa = 11.84SGTRR294 pKa = 11.84YY295 pKa = 10.06VEE297 pKa = 3.87TLKK300 pKa = 11.02AHH302 pKa = 6.67WGVTSPDD309 pKa = 3.12HH310 pKa = 6.28RR311 pKa = 11.84LQRR314 pKa = 11.84AEE316 pKa = 3.84FLGGGSSGINITPVAQTSGQTAPVDD341 pKa = 3.64ADD343 pKa = 3.78TMGMLAGFGTASGTHH358 pKa = 5.93GFSKK362 pKa = 10.8SFVEE366 pKa = 4.05HH367 pKa = 6.3GVLIGICNVRR377 pKa = 11.84GDD379 pKa = 3.41ITYY382 pKa = 10.37SQGIDD387 pKa = 3.53RR388 pKa = 11.84MWSKK392 pKa = 9.38STRR395 pKa = 11.84FDD397 pKa = 3.27FFIRR401 pKa = 11.84SSRR404 pKa = 11.84KK405 pKa = 8.43LASRR409 pKa = 11.84RR410 pKa = 11.84YY411 pKa = 9.16SIKK414 pKa = 10.62NMVGRR419 pKa = 11.84EE420 pKa = 4.12CEE422 pKa = 3.62RR423 pKa = 11.84SISVRR428 pKa = 11.84LSRR431 pKa = 11.84KK432 pKa = 6.65VCRR435 pKa = 11.84ISACTIAFMRR445 pKa = 11.84IVV447 pKa = 3.11

Molecular weight: 48.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9M201|M9M201_9VIRU Replication protein (Fragment) OS=uncultured marine virus OX=186617 PE=4 SV=1
DDD2 pKa = 4.72GGSLTWSDDD11 pKa = 3.38ALEEE15 pKa = 4.61DDD17 pKa = 4.87SAALAPNVNDDD28 pKa = 3.15RR29 pKa = 11.84LAFATQHHH37 pKa = 5.4LEEE40 pKa = 4.57DDD42 pKa = 3.38RR43 pKa = 11.84SGTRR47 pKa = 11.84YYY49 pKa = 9.92EEE51 pKa = 4.08LKKK54 pKa = 10.9RR55 pKa = 11.84WGVTSPDDD63 pKa = 3.1RR64 pKa = 11.84LQRR67 pKa = 11.84AEEE70 pKa = 3.84YY71 pKa = 10.06GGGSTRR77 pKa = 11.84INVAPVQQNTASTTPTSPAAQDDD100 pKa = 3.48KK101 pKa = 10.41GNLAGVGTANGTHHH115 pKa = 6.47WSKKK119 pKa = 10.94FVEEE123 pKa = 4.24HH124 pKa = 6.06VIIILGNLRR133 pKa = 11.84GDDD136 pKa = 3.2SYYY139 pKa = 10.53QGVDDD144 pKa = 3.47YYY146 pKa = 9.35SKKK149 pKa = 11.43NTLRR153 pKa = 11.84FRR155 pKa = 11.84ISRR158 pKa = 11.84NGKKK162 pKa = 7.78HH163 pKa = 5.77RR164 pKa = 11.84TSRR167 pKa = 11.84VKKK170 pKa = 10.35RR171 pKa = 11.84NLDDD175 pKa = 3.05HH176 pKa = 6.95HHH178 pKa = 5.49HHH180 pKa = 4.45CH

Molecular weight: 20.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1743

162

447

290.5

32.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.09 ± 0.468

1.721 ± 0.24

6.368 ± 0.458

3.729 ± 0.564

4.246 ± 0.623

7.803 ± 0.156

3.385 ± 0.477

6.024 ± 0.136

4.705 ± 0.661

7.057 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.328 ± 0.434

4.705 ± 0.256

4.59 ± 0.298

2.754 ± 0.153

8.032 ± 0.503

5.565 ± 1.012

6.942 ± 0.467

5.106 ± 0.381

2.811 ± 0.523

2.983 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski