Corvus brachyrhynchos (American crow)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091F5V0|A0A091F5V0_CORBR Scavenger receptor cysteine-rich domain-containing group B protein (Fragment) OS=Corvus brachyrhynchos OX=85066 GN=N302_16433 PE=4 SV=1
DDD2 pKa = 5.37DDD4 pKa = 3.8VAFCVLAADDD14 pKa = 4.63EEE16 pKa = 4.6DD17 pKa = 3.96EE18 pKa = 4.72DDD20 pKa = 3.31ALQIHHH26 pKa = 6.13TLIQAFCCEEE36 pKa = 3.78DDD38 pKa = 2.96DDD40 pKa = 3.95VRR42 pKa = 11.84VNDDD46 pKa = 3.46AKKK49 pKa = 10.32AAIVGPSEEE58 pKa = 5.03DD59 pKa = 3.31GEEE62 pKa = 4.1RR63 pKa = 11.84DDD65 pKa = 3.79HHH67 pKa = 6.98ILITV

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091ESA7|A0A091ESA7_CORBR Uncharacterized protein (Fragment) OS=Corvus brachyrhynchos OX=85066 GN=N302_06631 PE=4 SV=1
VV1 pKa = 7.07LRR3 pKa = 11.84PHH5 pKa = 7.1RR6 pKa = 11.84HH7 pKa = 5.05RR8 pKa = 11.84QLAALLVVQGALRR21 pKa = 11.84VQRR24 pKa = 11.84AAPRR28 pKa = 11.84LHH30 pKa = 5.99RR31 pKa = 11.84HH32 pKa = 3.76QRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84AAARR42 pKa = 11.84QPVAHH47 pKa = 6.81APVGARR53 pKa = 11.84VRR55 pKa = 11.84VPRR58 pKa = 11.84LHH60 pKa = 6.78AHH62 pKa = 5.39HH63 pKa = 5.88QRR65 pKa = 11.84ARR67 pKa = 11.84RR68 pKa = 11.84VVLRR72 pKa = 11.84HH73 pKa = 4.29VRR75 pKa = 11.84AVRR78 pKa = 11.84RR79 pKa = 11.84LLEE82 pKa = 3.66HH83 pKa = 6.33RR84 pKa = 11.84RR85 pKa = 11.84VVVHH89 pKa = 6.07VRR91 pKa = 11.84HH92 pKa = 6.12LQHH95 pKa = 6.4QLPAAPQRR103 pKa = 11.84RR104 pKa = 11.84PAPVPGHH111 pKa = 6.33HH112 pKa = 6.11RR113 pKa = 11.84HH114 pKa = 5.97AVLGHH119 pKa = 7.22LLTVQQ124 pKa = 3.36

Molecular weight: 14.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13621

0

13621

5900384

30

5555

433.2

48.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.502 ± 0.019

2.29 ± 0.02

5.017 ± 0.017

7.144 ± 0.025

3.912 ± 0.018

6.009 ± 0.027

2.59 ± 0.011

4.913 ± 0.015

6.305 ± 0.028

9.774 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.009

4.017 ± 0.013

5.395 ± 0.027

4.686 ± 0.018

5.252 ± 0.016

8.141 ± 0.028

5.338 ± 0.013

6.323 ± 0.018

1.223 ± 0.008

2.943 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski