Dragonfly associated cyclovirus 3
Average proteome isoelectric point is 8.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0A1K7|K0A1K7_9CIRC Spliced replication-associated protein OS=Dragonfly associated cyclovirus 3 OX=1234881 PE=4 SV=1
MM1 pKa = 7.9 PSPRR5 pKa = 11.84 DD6 pKa = 3.16 LKK8 pKa = 10.82 GAVRR12 pKa = 11.84 LGIYY16 pKa = 8.15 VLRR19 pKa = 11.84 CLYY22 pKa = 10.42 NGTMSARR29 pKa = 11.84 QRR31 pKa = 11.84 LKK33 pKa = 10.78 RR34 pKa = 11.84 IVFTLNNYY42 pKa = 10.06 CEE44 pKa = 4.55 DD45 pKa = 3.86 DD46 pKa = 3.88 FVRR49 pKa = 11.84 IQSYY53 pKa = 9.01 VEE55 pKa = 3.95 IYY57 pKa = 9.68 QYY59 pKa = 11.1 CVVGRR64 pKa = 11.84 EE65 pKa = 4.18 TAPEE69 pKa = 4.04 TGTPHH74 pKa = 6.13 LQGFINFKK82 pKa = 9.91 SKK84 pKa = 11.2 RR85 pKa = 11.84 EE86 pKa = 3.75 FGTIKK91 pKa = 10.07 TIVGDD96 pKa = 3.69 RR97 pKa = 11.84 AHH99 pKa = 7.14 IEE101 pKa = 3.85 PAKK104 pKa = 10.88 GSDD107 pKa = 3.6 VQNKK111 pKa = 8.23 EE112 pKa = 3.92 YY113 pKa = 9.25 CTKK116 pKa = 10.92 GGDD119 pKa = 3.41 YY120 pKa = 9.68 WEE122 pKa = 5.54 CGTPSGPGYY131 pKa = 10.44 RR132 pKa = 11.84 SDD134 pKa = 4.17 LADD137 pKa = 3.11 VVQTVKK143 pKa = 10.32 GAKK146 pKa = 9.34 RR147 pKa = 11.84 LRR149 pKa = 11.84 EE150 pKa = 3.89 VVEE153 pKa = 3.76 RR154 pKa = 11.84 HH155 pKa = 4.82 PCEE158 pKa = 3.95 FIKK161 pKa = 9.71 YY162 pKa = 9.32 HH163 pKa = 6.67 RR164 pKa = 11.84 GIEE167 pKa = 4.14 KK168 pKa = 10.41 LFGMLSEE175 pKa = 4.2 RR176 pKa = 11.84 EE177 pKa = 3.79 KK178 pKa = 11.16 RR179 pKa = 11.84 NWKK182 pKa = 8.47 TEE184 pKa = 4.04 TIVYY188 pKa = 10.28 YY189 pKa = 10.74 GDD191 pKa = 3.83 PGAGKK196 pKa = 9.66 SRR198 pKa = 11.84 KK199 pKa = 9.0 AAEE202 pKa = 3.91 VGAAAEE208 pKa = 4.23 GGVYY212 pKa = 10.49 YY213 pKa = 9.22 KK214 pKa = 10.39 TRR216 pKa = 11.84 GPWWDD221 pKa = 3.66 GYY223 pKa = 11.25 NGEE226 pKa = 4.39 TTVIVDD232 pKa = 5.39 DD233 pKa = 4.36 YY234 pKa = 10.71 YY235 pKa = 11.83 GWLAYY240 pKa = 10.53 DD241 pKa = 4.01 EE242 pKa = 4.4 VLKK245 pKa = 9.99 ITDD248 pKa = 3.59 RR249 pKa = 11.84 YY250 pKa = 10.33 LL251 pKa = 4.8
Molecular weight: 28.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.683
IPC2_protein 7.922
IPC_protein 7.834
Toseland 7.556
ProMoST 8.302
Dawson 8.507
Bjellqvist 8.741
Wikipedia 8.478
Rodwell 8.521
Grimsley 7.512
Solomon 8.624
Lehninger 8.609
Nozaki 8.799
DTASelect 8.521
Thurlkill 8.565
EMBOSS 8.682
Sillero 8.843
Patrickios 4.05
IPC_peptide 8.609
IPC2_peptide 7.658
IPC2.peptide.svr19 7.895
Protein with the highest isoelectric point:
>tr|K0A154|K0A154_9CIRC Replication-associated protein OS=Dragonfly associated cyclovirus 3 OX=1234881 PE=4 SV=1
MM1 pKa = 7.74 AYY3 pKa = 10.44 LRR5 pKa = 11.84 ARR7 pKa = 11.84 YY8 pKa = 6.89 TNRR11 pKa = 11.84 RR12 pKa = 11.84 FSRR15 pKa = 11.84 RR16 pKa = 11.84 GLRR19 pKa = 11.84 QVQRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 LIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 PRR34 pKa = 11.84 KK35 pKa = 9.96 SNLSCKK41 pKa = 9.1 FTTNLVLKK49 pKa = 8.44 QTTGIQYY56 pKa = 11.14 AHH58 pKa = 7.68 IAPALEE64 pKa = 4.82 DD65 pKa = 3.67 FAEE68 pKa = 4.42 TTNLITNFEE77 pKa = 4.39 YY78 pKa = 11.07 YY79 pKa = 10.45 KK80 pKa = 10.06 IRR82 pKa = 11.84 KK83 pKa = 8.51 VRR85 pKa = 11.84 ITVTPRR91 pKa = 11.84 NNVAYY96 pKa = 10.1 QGQTVPDD103 pKa = 3.75 YY104 pKa = 11.24 VIAPFHH110 pKa = 6.76 HH111 pKa = 6.77 PVDD114 pKa = 4.46 DD115 pKa = 4.33 KK116 pKa = 11.64 SISVDD121 pKa = 3.33 SLLTLDD127 pKa = 3.23 RR128 pKa = 11.84 HH129 pKa = 5.37 KK130 pKa = 10.66 KK131 pKa = 9.38 YY132 pKa = 10.59 RR133 pKa = 11.84 GTQRR137 pKa = 11.84 GHH139 pKa = 4.75 MTFKK143 pKa = 10.31 PAVIGLASTSLDD155 pKa = 3.7 DD156 pKa = 4.94 SSGTYY161 pKa = 9.1 ATMRR165 pKa = 11.84 WSPKK169 pKa = 10.12 ILITNNTTPQQVKK182 pKa = 9.72 HH183 pKa = 5.4 FCGLLAFAPQGSNAQEE199 pKa = 3.79 YY200 pKa = 10.07 EE201 pKa = 3.98 ITLDD205 pKa = 4.2 AYY207 pKa = 10.17 CTFYY211 pKa = 10.57 NQKK214 pKa = 9.89 INKK217 pKa = 8.94 LLL219 pKa = 3.84
Molecular weight: 25.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.882
IPC_protein 10.584
Toseland 10.482
ProMoST 10.218
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.921
Grimsley 10.73
Solomon 10.716
Lehninger 10.672
Nozaki 10.467
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.891
Sillero 10.57
Patrickios 10.57
IPC_peptide 10.716
IPC2_peptide 9.355
IPC2.peptide.svr19 8.409
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
770
219
300
256.7
29.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.714 ± 0.44
1.948 ± 0.229
4.935 ± 0.33
5.714 ± 1.265
3.377 ± 0.296
9.091 ± 1.823
2.078 ± 0.259
5.195 ± 0.3
6.494 ± 0.285
7.013 ± 0.692
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.169 ± 0.088
3.636 ± 0.672
4.286 ± 0.132
3.117 ± 0.868
8.571 ± 0.709
4.416 ± 0.425
7.662 ± 0.878
7.143 ± 0.773
1.688 ± 0.458
6.753 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here