Aeriscardovia aeriphila
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1287 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A261FC38|A0A261FC38_9BIFI DNA-binding protein OS=Aeriscardovia aeriphila OX=218139 GN=AEAE_0016 PE=4 SV=1
MM1 pKa = 7.61 PLHH4 pKa = 5.42 YY5 pKa = 9.71 TSVADD10 pKa = 5.69 AGIQPINLAQMRR22 pKa = 11.84 QDD24 pKa = 3.02 VHH26 pKa = 8.79 LDD28 pKa = 3.5 FTHH31 pKa = 7.03 AACLLSVEE39 pKa = 4.42 PEE41 pKa = 4.05 VVEE44 pKa = 3.85 RR45 pKa = 11.84 WEE47 pKa = 4.25 RR48 pKa = 11.84 NLEE51 pKa = 4.03 VLPVAVYY58 pKa = 9.99 KK59 pKa = 10.74 KK60 pKa = 10.63 YY61 pKa = 10.94 VATLEE66 pKa = 4.18 KK67 pKa = 10.38 AGRR70 pKa = 11.84 IYY72 pKa = 10.68 QQDD75 pKa = 3.72 PDD77 pKa = 4.01 NLIFTIDD84 pKa = 3.7 LASLDD89 pKa = 4.2 RR90 pKa = 11.84 LDD92 pKa = 5.53 HH93 pKa = 6.32 PMTYY97 pKa = 9.03 TACTEE102 pKa = 4.19 EE103 pKa = 4.39 YY104 pKa = 10.51 VEE106 pKa = 4.34 QVLALGVDD114 pKa = 4.49 FPEE117 pKa = 5.22 DD118 pKa = 3.53 FWPYY122 pKa = 10.46 EE123 pKa = 4.08 FVPEE127 pKa = 3.97 YY128 pKa = 10.33 PIVLRR133 pKa = 11.84 EE134 pKa = 3.9 QIAYY138 pKa = 5.6 EE139 pKa = 4.13 TEE141 pKa = 4.54 GIEE144 pKa = 4.2 PYY146 pKa = 10.35 PGFGEE151 pKa = 3.94 AWEE154 pKa = 4.37 TFQDD158 pKa = 3.81 NLDD161 pKa = 3.79 EE162 pKa = 4.42 VDD164 pKa = 4.65 SIVHH168 pKa = 6.43 QILADD173 pKa = 3.42 QGGKK177 pKa = 9.22 VLYY180 pKa = 10.6 DD181 pKa = 3.81 DD182 pKa = 5.28 DD183 pKa = 6.54 DD184 pKa = 5.21 YY185 pKa = 12.07 YY186 pKa = 11.63 GYY188 pKa = 10.86 DD189 pKa = 4.07 LPDD192 pKa = 3.45 GVNFEE197 pKa = 4.36 GDD199 pKa = 3.74 SVALADD205 pKa = 5.05 LARR208 pKa = 11.84 EE209 pKa = 4.12 NNTFDD214 pKa = 5.45 DD215 pKa = 5.58 DD216 pKa = 4.47 STFDD220 pKa = 5.88 DD221 pKa = 5.46 DD222 pKa = 6.61 DD223 pKa = 6.11 FDD225 pKa = 6.61 DD226 pKa = 5.83 GSTFNDD232 pKa = 3.89 GDD234 pKa = 3.94 FEE236 pKa = 4.69 DD237 pKa = 6.02 ANDD240 pKa = 4.06 ADD242 pKa = 4.97 DD243 pKa = 5.82 DD244 pKa = 4.49 FSEE247 pKa = 5.22 SYY249 pKa = 10.74 TDD251 pKa = 4.6 ANFADD256 pKa = 5.51 FAARR260 pKa = 11.84 LSQFSSGQEE269 pKa = 3.66 DD270 pKa = 4.25 GAPSLRR276 pKa = 11.84 YY277 pKa = 10.12 DD278 pKa = 3.04 NGAIHH283 pKa = 6.73 FEE285 pKa = 4.32 VVDD288 pKa = 4.48 LDD290 pKa = 3.78 AGKK293 pKa = 10.18 DD294 pKa = 3.27 ADD296 pKa = 4.13 TQTDD300 pKa = 3.32 TGATRR305 pKa = 11.84 SAALDD310 pKa = 3.67 ADD312 pKa = 4.28 TNADD316 pKa = 3.33 AVTSANVNANAEE328 pKa = 4.26 ANPIPITSARR338 pKa = 11.84 IQRR341 pKa = 11.84 GDD343 pKa = 3.21 GSLSASHH350 pKa = 5.93 STPPSEE356 pKa = 4.41
Molecular weight: 39.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.63
IPC_protein 3.681
Toseland 3.439
ProMoST 3.834
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.503
Grimsley 3.35
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.05
Thurlkill 3.503
EMBOSS 3.63
Sillero 3.795
Patrickios 1.85
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A261F7X0|A0A261F7X0_9BIFI Ammonium transporter OS=Aeriscardovia aeriphila OX=218139 GN=AEAE_1215 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.8 MKK15 pKa = 9.45 HH16 pKa = 5.74 GFRR19 pKa = 11.84 EE20 pKa = 4.06 RR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 ALINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.455
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.369
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.119
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1287
0
1287
474780
29
1774
368.9
40.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.335 ± 0.074
0.748 ± 0.02
5.905 ± 0.066
5.952 ± 0.068
3.519 ± 0.045
6.982 ± 0.061
2.45 ± 0.033
5.083 ± 0.056
4.327 ± 0.068
9.204 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.42 ± 0.031
3.699 ± 0.047
4.604 ± 0.051
4.629 ± 0.059
5.573 ± 0.068
7.195 ± 0.072
5.897 ± 0.054
7.381 ± 0.061
1.406 ± 0.026
2.69 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here