Ruminococcus sp. AM42-11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; unclassified Ruminococcus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417CV03|A0A417CV03_9FIRM HNH endonuclease OS=Ruminococcus sp. AM42-11 OX=2292372 GN=DW904_10695 PE=4 SV=1
MM1 pKa = 7.41LAGMTLCAVPVMAEE15 pKa = 3.92DD16 pKa = 4.33AEE18 pKa = 4.57TPEE21 pKa = 4.46NEE23 pKa = 4.26VTGDD27 pKa = 3.45ASADD31 pKa = 3.41DD32 pKa = 4.65SFVVWGWNDD41 pKa = 4.05DD42 pKa = 3.25IKK44 pKa = 11.21KK45 pKa = 10.34ILDD48 pKa = 3.8GPFKK52 pKa = 10.32EE53 pKa = 5.41AYY55 pKa = 8.5PDD57 pKa = 3.59DD58 pKa = 3.97YY59 pKa = 11.7EE60 pKa = 6.05RR61 pKa = 11.84IVFVNTGGSDD71 pKa = 3.99YY72 pKa = 10.65YY73 pKa = 10.72QSKK76 pKa = 10.47LDD78 pKa = 4.18PVLDD82 pKa = 4.33DD83 pKa = 4.97PSNEE87 pKa = 4.6LYY89 pKa = 10.16PDD91 pKa = 3.54MMGLEE96 pKa = 4.0VDD98 pKa = 4.13YY99 pKa = 10.89VQKK102 pKa = 10.79YY103 pKa = 8.64VNSDD107 pKa = 2.59WVQNVADD114 pKa = 4.84LGITADD120 pKa = 4.71DD121 pKa = 3.97YY122 pKa = 12.15ANSYY126 pKa = 10.0QYY128 pKa = 11.64NLDD131 pKa = 3.92LGSDD135 pKa = 3.14VDD137 pKa = 4.12GNVRR141 pKa = 11.84ALFWQATPGCYY152 pKa = 9.5AVRR155 pKa = 11.84ADD157 pKa = 4.6LAEE160 pKa = 4.86KK161 pKa = 10.91YY162 pKa = 10.74LGTTDD167 pKa = 3.67PAALAEE173 pKa = 4.31KK174 pKa = 10.35FKK176 pKa = 11.4DD177 pKa = 3.2LDD179 pKa = 4.3TIVATAKK186 pKa = 9.95EE187 pKa = 4.32VNDD190 pKa = 4.27ASGGKK195 pKa = 9.74CKK197 pKa = 10.42LFSGYY202 pKa = 10.71DD203 pKa = 3.42EE204 pKa = 4.74IKK206 pKa = 10.56RR207 pKa = 11.84SLTGSRR213 pKa = 11.84TQGFYY218 pKa = 11.22DD219 pKa = 4.09EE220 pKa = 4.85NDD222 pKa = 3.99KK223 pKa = 9.84ITIDD227 pKa = 4.45DD228 pKa = 4.3NIKK231 pKa = 10.09TYY233 pKa = 10.45MEE235 pKa = 4.22TAKK238 pKa = 9.92TLYY241 pKa = 10.93DD242 pKa = 3.65EE243 pKa = 6.02DD244 pKa = 4.05LTFNTDD250 pKa = 2.19QWSADD255 pKa = 2.68WSANMSGDD263 pKa = 3.64GVDD266 pKa = 3.89SNAALAYY273 pKa = 8.12MGCPWFVYY281 pKa = 9.68WSLSDD286 pKa = 3.06AWKK289 pKa = 10.88GNTILVPTQTKK300 pKa = 9.85CYY302 pKa = 9.28WGGTGLAATTDD313 pKa = 3.87CADD316 pKa = 3.52TEE318 pKa = 4.2LAAKK322 pKa = 9.88IMKK325 pKa = 9.78FFTCDD330 pKa = 3.01EE331 pKa = 4.75DD332 pKa = 4.96GMVAINALNSDD343 pKa = 4.24YY344 pKa = 11.58VNNTAAVSKK353 pKa = 10.13IIEE356 pKa = 4.73AGTSADD362 pKa = 3.52GNGYY366 pKa = 10.13LYY368 pKa = 10.15PDD370 pKa = 4.25AGQNFMEE377 pKa = 5.18FFLPLADD384 pKa = 4.62GLDD387 pKa = 3.5ASMVTAEE394 pKa = 4.37DD395 pKa = 3.72QQILSLMDD403 pKa = 3.66TQTKK407 pKa = 10.37AYY409 pKa = 8.2ATGEE413 pKa = 4.11KK414 pKa = 10.76DD415 pKa = 4.75LDD417 pKa = 3.67TALADD422 pKa = 3.84LTASIHH428 pKa = 6.09DD429 pKa = 3.83TFSYY433 pKa = 10.81LSAEE437 pKa = 4.21

Molecular weight: 47.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417CSJ0|A0A417CSJ0_9FIRM DUF4446 family protein OS=Ruminococcus sp. AM42-11 OX=2292372 GN=DW904_11800 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3917

0

3917

1165353

24

2560

297.5

33.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.274 ± 0.039

1.564 ± 0.02

5.74 ± 0.041

7.676 ± 0.047

4.068 ± 0.025

6.989 ± 0.041

1.72 ± 0.019

7.044 ± 0.031

7.22 ± 0.036

8.772 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.09 ± 0.022

4.524 ± 0.028

3.238 ± 0.024

3.385 ± 0.024

4.2 ± 0.035

5.842 ± 0.036

5.583 ± 0.046

6.778 ± 0.036

1.002 ± 0.016

4.292 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski