Betapapillomavirus 4
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AL17|A0A2D2AL17_9PAPI Regulatory protein E2 OS=Betapapillomavirus 4 OX=334208 GN=E2 PE=3 SV=1
MM1 pKa = 7.6 IGKK4 pKa = 8.64 QATIPEE10 pKa = 4.39 IVLDD14 pKa = 4.27 LQDD17 pKa = 4.12 LVQPIDD23 pKa = 3.44 LHH25 pKa = 8.22 CDD27 pKa = 3.25 EE28 pKa = 5.03 EE29 pKa = 4.89 LEE31 pKa = 4.28 NQVEE35 pKa = 4.37 EE36 pKa = 4.62 EE37 pKa = 4.23 PAPQRR42 pKa = 11.84 IDD44 pKa = 3.45 YY45 pKa = 10.25 KK46 pKa = 10.65 IVSSCGGCGIKK57 pKa = 10.43 LRR59 pKa = 11.84 IFATCTQFGIRR70 pKa = 11.84 TLQDD74 pKa = 3.47 LLLEE78 pKa = 4.9 EE79 pKa = 4.78 IALLCPDD86 pKa = 4.81 CKK88 pKa = 10.68 NGRR91 pKa = 3.72
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.326
IPC2_protein 4.418
IPC_protein 4.291
Toseland 4.126
ProMoST 4.418
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.126
Rodwell 4.139
Grimsley 4.05
Solomon 4.24
Lehninger 4.19
Nozaki 4.368
DTASelect 4.507
Thurlkill 4.151
EMBOSS 4.151
Sillero 4.406
Patrickios 3.325
IPC_peptide 4.24
IPC2_peptide 4.393
IPC2.peptide.svr19 4.322
Protein with the highest isoelectric point:
>tr|A0A2D2AL52|A0A2D2AL52_9PAPI Protein E6 OS=Betapapillomavirus 4 OX=334208 GN=E6 PE=3 SV=1
MM1 pKa = 7.5 EE2 pKa = 5.42 ALSDD6 pKa = 4.12 RR7 pKa = 11.84 FNALQDD13 pKa = 3.51 KK14 pKa = 10.97 LMTIYY19 pKa = 10.57 EE20 pKa = 4.33 KK21 pKa = 10.81 ASEE24 pKa = 4.26 SLTDD28 pKa = 5.54 QIEE31 pKa = 4.11 HH32 pKa = 6.5 WNLLRR37 pKa = 11.84 QEE39 pKa = 4.21 QVLFHH44 pKa = 6.04 YY45 pKa = 10.25 ARR47 pKa = 11.84 QRR49 pKa = 11.84 GVMRR53 pKa = 11.84 LGYY56 pKa = 10.1 QPVPALTISEE66 pKa = 4.36 AKK68 pKa = 10.21 AKK70 pKa = 10.0 EE71 pKa = 4.12 AIAMVLHH78 pKa = 6.59 LEE80 pKa = 4.11 ALQRR84 pKa = 11.84 SPYY87 pKa = 10.01 KK88 pKa = 10.23 DD89 pKa = 3.66 EE90 pKa = 4.24 KK91 pKa = 8.2 WTLVNTSVEE100 pKa = 4.39 TFRR103 pKa = 11.84 TPPEE107 pKa = 3.76 NCFKK111 pKa = 10.82 KK112 pKa = 10.56 GPKK115 pKa = 9.09 TIEE118 pKa = 3.58 IVYY121 pKa = 10.4 DD122 pKa = 3.78 GNPDD126 pKa = 2.96 NTMLYY131 pKa = 9.05 TIWTFIYY138 pKa = 11.02 YY139 pKa = 10.31 EE140 pKa = 4.44 DD141 pKa = 6.11 DD142 pKa = 3.26 EE143 pKa = 5.95 GNWQKK148 pKa = 9.59 TEE150 pKa = 3.87 GQLDD154 pKa = 3.69 YY155 pKa = 11.36 HH156 pKa = 6.29 GAYY159 pKa = 10.5 YY160 pKa = 10.95 LDD162 pKa = 4.19 GLNKK166 pKa = 9.71 QYY168 pKa = 10.88 YY169 pKa = 9.58 IRR171 pKa = 11.84 FAQDD175 pKa = 2.32 ARR177 pKa = 11.84 RR178 pKa = 11.84 YY179 pKa = 9.75 SEE181 pKa = 3.82 TGEE184 pKa = 4.01 WEE186 pKa = 4.2 VKK188 pKa = 10.11 FNNEE192 pKa = 3.3 ILFAPVTSSTNSEE205 pKa = 4.13 EE206 pKa = 4.22 EE207 pKa = 3.85 RR208 pKa = 11.84 DD209 pKa = 3.94 RR210 pKa = 11.84 PAPASDD216 pKa = 4.23 PGSLPQTSGRR226 pKa = 11.84 LSPSPVQRR234 pKa = 11.84 KK235 pKa = 7.21 QLSKK239 pKa = 10.48 RR240 pKa = 11.84 RR241 pKa = 11.84 YY242 pKa = 7.71 GRR244 pKa = 11.84 KK245 pKa = 9.65 DD246 pKa = 3.22 SSPTATSRR254 pKa = 11.84 VQRR257 pKa = 11.84 RR258 pKa = 11.84 PKK260 pKa = 10.18 ASPRR264 pKa = 11.84 RR265 pKa = 11.84 SRR267 pKa = 11.84 SRR269 pKa = 11.84 SGSRR273 pKa = 11.84 SGSQGDD279 pKa = 3.62 ARR281 pKa = 11.84 TLLSLRR287 pKa = 11.84 RR288 pKa = 11.84 GEE290 pKa = 4.1 RR291 pKa = 11.84 EE292 pKa = 3.63 RR293 pKa = 11.84 GEE295 pKa = 4.04 GRR297 pKa = 11.84 GTGIRR302 pKa = 11.84 VRR304 pKa = 11.84 GRR306 pKa = 11.84 SGSRR310 pKa = 11.84 GRR312 pKa = 11.84 SRR314 pKa = 11.84 SRR316 pKa = 11.84 SRR318 pKa = 11.84 SRR320 pKa = 11.84 SRR322 pKa = 11.84 SRR324 pKa = 11.84 SRR326 pKa = 11.84 SRR328 pKa = 11.84 SSRR331 pKa = 11.84 SRR333 pKa = 11.84 GRR335 pKa = 11.84 GNTTTRR341 pKa = 11.84 GRR343 pKa = 11.84 GGYY346 pKa = 7.01 RR347 pKa = 11.84 TRR349 pKa = 11.84 RR350 pKa = 11.84 SRR352 pKa = 11.84 SKK354 pKa = 11.01 SVDD357 pKa = 3.07 TSGIPPDD364 pKa = 3.38 QVGRR368 pKa = 11.84 SLQSVGRR375 pKa = 11.84 QHH377 pKa = 6.97 SGRR380 pKa = 11.84 LARR383 pKa = 11.84 LLDD386 pKa = 3.84 EE387 pKa = 5.59 ARR389 pKa = 11.84 DD390 pKa = 3.77 PPVILLKK397 pKa = 11.05 GKK399 pKa = 10.59 ANTLKK404 pKa = 10.49 CYY406 pKa = 10.29 RR407 pKa = 11.84 YY408 pKa = 9.45 RR409 pKa = 11.84 AKK411 pKa = 10.46 EE412 pKa = 3.99 KK413 pKa = 10.72 YY414 pKa = 8.94 KK415 pKa = 10.68 GYY417 pKa = 9.79 YY418 pKa = 10.1 DD419 pKa = 3.54 RR420 pKa = 11.84 VSTTWSWVGAGSNDD434 pKa = 3.64 RR435 pKa = 11.84 IGRR438 pKa = 11.84 SRR440 pKa = 11.84 MLFSFTSKK448 pKa = 9.95 SQRR451 pKa = 11.84 QMFLNIMKK459 pKa = 10.12 LPKK462 pKa = 10.5 GVDD465 pKa = 2.93 WSFGCFDD472 pKa = 4.93 SII474 pKa = 5.05
Molecular weight: 54.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.333
IPC2_protein 9.765
IPC_protein 10.628
Toseland 10.613
ProMoST 10.365
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.906
Grimsley 10.804
Solomon 10.847
Lehninger 10.804
Nozaki 10.584
DTASelect 10.467
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.555
IPC_peptide 10.847
IPC2_peptide 9.414
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2554
91
612
364.9
41.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.951 ± 0.4
2.271 ± 0.86
6.46 ± 0.427
6.852 ± 0.545
3.876 ± 0.425
6.891 ± 0.792
1.879 ± 0.257
4.777 ± 0.654
5.442 ± 0.789
8.614 ± 0.78
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.331 ± 0.281
4.15 ± 0.551
5.873 ± 0.778
4.62 ± 0.282
7.009 ± 1.4
7.244 ± 1.023
6.147 ± 0.908
5.912 ± 0.679
1.253 ± 0.292
3.446 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here