Desulfococcus multivorans DSM 2059
Average proteome isoelectric point is 6.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3844 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S7UXY4|S7UXY4_DESML TPR_REGION domain-containing protein OS=Desulfococcus multivorans DSM 2059 OX=1121405 GN=dsmv_2765 PE=4 SV=1
MM1 pKa = 7.68 AEE3 pKa = 4.0 KK4 pKa = 10.81 NMNRR8 pKa = 11.84 WEE10 pKa = 4.46 CPCGYY15 pKa = 9.82 IYY17 pKa = 10.81 DD18 pKa = 4.24 PAEE21 pKa = 4.2 GDD23 pKa = 3.49 AEE25 pKa = 4.24 NNILVGTSFDD35 pKa = 5.03 DD36 pKa = 6.16 LPDD39 pKa = 3.95 DD40 pKa = 3.81 WTCPKK45 pKa = 10.47 CGAEE49 pKa = 3.94 KK50 pKa = 10.82 EE51 pKa = 4.34 FFEE54 pKa = 4.63 EE55 pKa = 5.53 LRR57 pKa = 11.84 DD58 pKa = 3.68
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.948
IPC_protein 3.846
Toseland 3.656
ProMoST 3.91
Dawson 3.821
Bjellqvist 4.05
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.579
Solomon 3.808
Lehninger 3.757
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.961
Patrickios 1.888
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|S7TDM7|S7TDM7_DESML Dynamin family protein OS=Desulfococcus multivorans DSM 2059 OX=1121405 GN=dsmv_3262 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.17 QPSKK9 pKa = 9.15 IKK11 pKa = 10.36 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 6.37 GFLKK20 pKa = 10.63 RR21 pKa = 11.84 MSTKK25 pKa = 10.39 AGRR28 pKa = 11.84 NIIKK32 pKa = 10.23 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.88 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3844
0
3844
1279499
30
2917
332.9
36.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.379 ± 0.044
1.224 ± 0.021
5.769 ± 0.03
6.247 ± 0.034
4.224 ± 0.026
7.868 ± 0.035
2.143 ± 0.02
6.516 ± 0.027
4.603 ± 0.04
9.696 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.752 ± 0.021
3.116 ± 0.024
4.864 ± 0.028
2.832 ± 0.02
7.102 ± 0.036
5.282 ± 0.027
5.236 ± 0.027
7.206 ± 0.032
1.15 ± 0.014
2.793 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here