Ruminococcus sp. CAG:624

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7MSH4|R7MSH4_9FIRM Malonyl CoA-acyl carrier protein transacylase OS=Ruminococcus sp. CAG:624 OX=1262965 GN=BN739_01946 PE=3 SV=1
MM1 pKa = 7.26NKK3 pKa = 9.73SRR5 pKa = 11.84ILTIILTALICVSAVSCNKK24 pKa = 9.97NSNKK28 pKa = 9.98SSEE31 pKa = 4.09NSSNEE36 pKa = 3.29ASQFATEE43 pKa = 4.21TTEE46 pKa = 3.98EE47 pKa = 4.26SSEE50 pKa = 4.06QTTEE54 pKa = 3.89EE55 pKa = 3.88QTDD58 pKa = 3.99TEE60 pKa = 4.38EE61 pKa = 4.35STDD64 pKa = 3.79SKK66 pKa = 11.54NEE68 pKa = 4.04SPDD71 pKa = 3.35NPFGKK76 pKa = 8.35TADD79 pKa = 3.37INDD82 pKa = 4.25YY83 pKa = 7.4FTPDD87 pKa = 3.37APEE90 pKa = 4.07PALWKK95 pKa = 9.61VTDD98 pKa = 4.37DD99 pKa = 3.92DD100 pKa = 4.68SGNVMYY106 pKa = 10.6MMGTIHH112 pKa = 6.32VASDD116 pKa = 3.35NSFSLPDD123 pKa = 4.09YY124 pKa = 11.42IMDD127 pKa = 4.36AYY129 pKa = 10.51EE130 pKa = 4.15NADD133 pKa = 3.51GVAVEE138 pKa = 3.92YY139 pKa = 10.56DD140 pKa = 3.38INKK143 pKa = 9.79LSSSLTSLALYY154 pKa = 10.51QNALLYY160 pKa = 10.67KK161 pKa = 10.62DD162 pKa = 3.23GTTVKK167 pKa = 10.53DD168 pKa = 3.59HH169 pKa = 6.96LSSEE173 pKa = 4.58TYY175 pKa = 10.15EE176 pKa = 3.95KK177 pKa = 10.93AKK179 pKa = 10.97NYY181 pKa = 10.04FDD183 pKa = 5.29DD184 pKa = 4.49LGVYY188 pKa = 10.02NEE190 pKa = 4.39QLDD193 pKa = 4.15KK194 pKa = 10.9YY195 pKa = 7.35TTGYY199 pKa = 9.7WIQQLNMIMFQRR211 pKa = 11.84MEE213 pKa = 4.24NLNLTGIDD221 pKa = 3.52SYY223 pKa = 11.62FIGLANKK230 pKa = 9.78DD231 pKa = 3.33GKK233 pKa = 10.32EE234 pKa = 3.97VVSIEE239 pKa = 4.33EE240 pKa = 3.96ISAQTDD246 pKa = 3.28ALNAYY251 pKa = 10.34SDD253 pKa = 4.43DD254 pKa = 3.76LADD257 pKa = 3.84YY258 pKa = 10.14VISDD262 pKa = 3.64MVDD265 pKa = 3.26NIDD268 pKa = 4.18EE269 pKa = 4.42IEE271 pKa = 4.66EE272 pKa = 4.25YY273 pKa = 8.36TQSVADD279 pKa = 5.22MYY281 pKa = 11.49NCWAKK286 pKa = 11.18GDD288 pKa = 3.67VDD290 pKa = 4.13KK291 pKa = 11.37LQEE294 pKa = 3.83IDD296 pKa = 3.47ADD298 pKa = 4.05EE299 pKa = 4.36MGEE302 pKa = 4.1LPKK305 pKa = 10.56EE306 pKa = 4.17LEE308 pKa = 4.22DD309 pKa = 6.49DD310 pKa = 3.86YY311 pKa = 11.94VQYY314 pKa = 11.44EE315 pKa = 4.19NIILHH320 pKa = 6.0NRR322 pKa = 11.84NQVMAQRR329 pKa = 11.84ASEE332 pKa = 4.39FIEE335 pKa = 4.61NNDD338 pKa = 3.26NLFYY342 pKa = 10.57MVGSLHH348 pKa = 6.41FAGDD352 pKa = 3.43QGVDD356 pKa = 3.9DD357 pKa = 5.1LLEE360 pKa = 4.81DD361 pKa = 3.01MGYY364 pKa = 7.96TVEE367 pKa = 4.69KK368 pKa = 10.58LHH370 pKa = 7.04

Molecular weight: 41.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7MQF4|R7MQF4_9FIRM 50S ribosomal protein L10 OS=Ruminococcus sp. CAG:624 OX=1262965 GN=rplJ PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.14YY3 pKa = 11.04VLIALIKK10 pKa = 10.17LYY12 pKa = 10.62RR13 pKa = 11.84KK14 pKa = 10.0LISPLFPPRR23 pKa = 11.84CKK25 pKa = 10.55YY26 pKa = 10.9YY27 pKa = 8.29PTCSSYY33 pKa = 11.48ALKK36 pKa = 10.55AVEE39 pKa = 3.69RR40 pKa = 11.84FGFFRR45 pKa = 11.84GTALAVWRR53 pKa = 11.84ILRR56 pKa = 11.84CNPWSLGGIDD66 pKa = 3.94PVPEE70 pKa = 3.69RR71 pKa = 11.84FSFKK75 pKa = 10.48RR76 pKa = 11.84NRR78 pKa = 3.34

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1963

0

1963

571658

31

1910

291.2

32.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.989 ± 0.054

1.749 ± 0.023

5.979 ± 0.047

7.145 ± 0.059

4.371 ± 0.041

6.434 ± 0.05

1.515 ± 0.019

8.58 ± 0.048

7.599 ± 0.043

8.296 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.754 ± 0.028

5.546 ± 0.051

3.036 ± 0.03

2.557 ± 0.031

3.669 ± 0.042

7.096 ± 0.054

5.413 ± 0.046

6.39 ± 0.045

0.679 ± 0.016

4.182 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski