Amino acid dipepetide frequency for Ruminococcus sp. CAG:624

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.444AlaAla: 6.444 ± 0.126
1.036AlaCys: 1.036 ± 0.041
5.029AlaAsp: 5.029 ± 0.097
5.991AlaGlu: 5.991 ± 0.115
3.021AlaPhe: 3.021 ± 0.084
4.436AlaGly: 4.436 ± 0.1
0.978AlaHis: 0.978 ± 0.042
4.919AlaIle: 4.919 ± 0.101
4.73AlaLys: 4.73 ± 0.094
6.014AlaLeu: 6.014 ± 0.117
1.984AlaMet: 1.984 ± 0.068
2.823AlaAsn: 2.823 ± 0.071
1.798AlaPro: 1.798 ± 0.061
1.886AlaGln: 1.886 ± 0.055
2.162AlaArg: 2.162 ± 0.068
4.261AlaSer: 4.261 ± 0.098
2.512AlaThr: 2.512 ± 0.091
7.025AlaVal: 7.025 ± 0.124
0.392AlaTrp: 0.392 ± 0.028
2.442AlaTyr: 2.442 ± 0.066
0.005AlaXaa: 0.005 ± 0.003
Cys
1.128CysAla: 1.128 ± 0.046
0.411CysCys: 0.411 ± 0.028
1.111CysAsp: 1.111 ± 0.053
1.128CysGlu: 1.128 ± 0.048
0.878CysPhe: 0.878 ± 0.039
1.763CysGly: 1.763 ± 0.054
0.341CysHis: 0.341 ± 0.023
1.454CysIle: 1.454 ± 0.054
1.092CysLys: 1.092 ± 0.041
1.142CysLeu: 1.142 ± 0.043
0.359CysMet: 0.359 ± 0.024
0.749CysAsn: 0.749 ± 0.04
0.712CysPro: 0.712 ± 0.043
0.39CysGln: 0.39 ± 0.026
0.785CysArg: 0.785 ± 0.035
1.436CysSer: 1.436 ± 0.055
0.896CysThr: 0.896 ± 0.048
0.931CysVal: 0.931 ± 0.038
0.122CysTrp: 0.122 ± 0.014
0.656CysTyr: 0.656 ± 0.032
0.002CysXaa: 0.002 ± 0.002
Asp
4.076AspAla: 4.076 ± 0.102
1.134AspCys: 1.134 ± 0.046
3.707AspAsp: 3.707 ± 0.103
5.005AspGlu: 5.005 ± 0.111
3.112AspPhe: 3.112 ± 0.085
4.083AspGly: 4.083 ± 0.104
0.604AspHis: 0.604 ± 0.029
5.83AspIle: 5.83 ± 0.111
4.795AspLys: 4.795 ± 0.091
4.025AspLeu: 4.025 ± 0.085
1.807AspMet: 1.807 ± 0.057
3.366AspAsn: 3.366 ± 0.083
1.335AspPro: 1.335 ± 0.048
0.745AspGln: 0.745 ± 0.034
1.88AspArg: 1.88 ± 0.061
3.796AspSer: 3.796 ± 0.089
3.142AspThr: 3.142 ± 0.073
3.827AspVal: 3.827 ± 0.094
0.436AspTrp: 0.436 ± 0.034
3.088AspTyr: 3.088 ± 0.072
0.002AspXaa: 0.002 ± 0.002
Glu
4.571GluAla: 4.571 ± 0.089
1.078GluCys: 1.078 ± 0.041
3.341GluAsp: 3.341 ± 0.082
4.93GluGlu: 4.93 ± 0.117
2.843GluPhe: 2.843 ± 0.08
3.282GluGly: 3.282 ± 0.077
1.13GluHis: 1.13 ± 0.043
6.59GluIle: 6.59 ± 0.11
6.906GluLys: 6.906 ± 0.12
6.873GluLeu: 6.873 ± 0.121
2.323GluMet: 2.323 ± 0.067
5.344GluAsn: 5.344 ± 0.093
1.676GluPro: 1.676 ± 0.058
2.302GluGln: 2.302 ± 0.074
3.009GluArg: 3.009 ± 0.071
4.092GluSer: 4.092 ± 0.084
3.588GluThr: 3.588 ± 0.083
3.824GluVal: 3.824 ± 0.087
0.476GluTrp: 0.476 ± 0.029
3.269GluTyr: 3.269 ± 0.094
0.005GluXaa: 0.005 ± 0.003
Phe
2.83PheAla: 2.83 ± 0.076
0.771PheCys: 0.771 ± 0.045
3.033PheAsp: 3.033 ± 0.072
3.018PheGlu: 3.018 ± 0.077
2.006PhePhe: 2.006 ± 0.067
3.199PheGly: 3.199 ± 0.076
0.584PheHis: 0.584 ± 0.033
3.74PheIle: 3.74 ± 0.105
2.862PheLys: 2.862 ± 0.074
3.411PheLeu: 3.411 ± 0.096
1.226PheMet: 1.226 ± 0.053
2.32PheAsn: 2.32 ± 0.065
1.204PhePro: 1.204 ± 0.047
0.862PheGln: 0.862 ± 0.038
1.487PheArg: 1.487 ± 0.046
3.696PheSer: 3.696 ± 0.09
2.561PheThr: 2.561 ± 0.055
2.708PheVal: 2.708 ± 0.072
0.338PheTrp: 0.338 ± 0.024
1.853PheTyr: 1.853 ± 0.055
0.003PheXaa: 0.003 ± 0.003
Gly
4.109GlyAla: 4.109 ± 0.101
1.361GlyCys: 1.361 ± 0.057
3.583GlyAsp: 3.583 ± 0.1
4.151GlyGlu: 4.151 ± 0.096
3.119GlyPhe: 3.119 ± 0.07
4.354GlyGly: 4.354 ± 0.101
1.137GlyHis: 1.137 ± 0.045
6.091GlyIle: 6.091 ± 0.114
5.134GlyLys: 5.134 ± 0.099
5.195GlyLeu: 5.195 ± 0.112
1.86GlyMet: 1.86 ± 0.059
3.297GlyAsn: 3.297 ± 0.066
0.938GlyPro: 0.938 ± 0.037
1.525GlyGln: 1.525 ± 0.05
2.503GlyArg: 2.503 ± 0.072
4.151GlySer: 4.151 ± 0.089
3.938GlyThr: 3.938 ± 0.097
4.368GlyVal: 4.368 ± 0.103
0.471GlyTrp: 0.471 ± 0.031
3.049GlyTyr: 3.049 ± 0.075
0.01GlyXaa: 0.01 ± 0.004
His
0.871HisAla: 0.871 ± 0.043
0.31HisCys: 0.31 ± 0.025
0.843HisAsp: 0.843 ± 0.04
0.95HisGlu: 0.95 ± 0.05
0.838HisPhe: 0.838 ± 0.041
1.088HisGly: 1.088 ± 0.041
0.297HisHis: 0.297 ± 0.026
1.322HisIle: 1.322 ± 0.057
1.09HisLys: 1.09 ± 0.043
1.169HisLeu: 1.169 ± 0.047
0.283HisMet: 0.283 ± 0.022
0.89HisAsn: 0.89 ± 0.044
0.604HisPro: 0.604 ± 0.035
0.395HisGln: 0.395 ± 0.028
0.638HisArg: 0.638 ± 0.031
1.044HisSer: 1.044 ± 0.045
0.911HisThr: 0.911 ± 0.041
0.759HisVal: 0.759 ± 0.037
0.122HisTrp: 0.122 ± 0.015
0.724HisTyr: 0.724 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.165IleAla: 6.165 ± 0.115
1.655IleCys: 1.655 ± 0.06
5.22IleAsp: 5.22 ± 0.093
5.692IleGlu: 5.692 ± 0.106
3.586IlePhe: 3.586 ± 0.088
5.367IleGly: 5.367 ± 0.105
1.251IleHis: 1.251 ± 0.044
7.506IleIle: 7.506 ± 0.132
6.245IleLys: 6.245 ± 0.106
6.751IleLeu: 6.751 ± 0.115
2.173IleMet: 2.173 ± 0.066
4.944IleAsn: 4.944 ± 0.099
3.184IlePro: 3.184 ± 0.086
1.922IleGln: 1.922 ± 0.064
3.205IleArg: 3.205 ± 0.075
7.12IleSer: 7.12 ± 0.118
5.003IleThr: 5.003 ± 0.093
5.073IleVal: 5.073 ± 0.106
0.53IleTrp: 0.53 ± 0.03
3.201IleTyr: 3.201 ± 0.076
0.005IleXaa: 0.005 ± 0.003
Lys
5.46LysAla: 5.46 ± 0.109
1.148LysCys: 1.148 ± 0.051
3.548LysAsp: 3.548 ± 0.091
5.437LysGlu: 5.437 ± 0.12
2.689LysPhe: 2.689 ± 0.064
4.293LysGly: 4.293 ± 0.084
1.148LysHis: 1.148 ± 0.049
6.544LysIle: 6.544 ± 0.115
6.765LysLys: 6.765 ± 0.124
6.71LysLeu: 6.71 ± 0.106
2.325LysMet: 2.325 ± 0.06
5.001LysAsn: 5.001 ± 0.117
2.167LysPro: 2.167 ± 0.077
2.106LysGln: 2.106 ± 0.066
3.121LysArg: 3.121 ± 0.078
5.208LysSer: 5.208 ± 0.105
4.093LysThr: 4.093 ± 0.083
4.153LysVal: 4.153 ± 0.091
0.553LysTrp: 0.553 ± 0.035
3.513LysTyr: 3.513 ± 0.079
0.007LysXaa: 0.007 ± 0.003
Leu
5.575LeuAla: 5.575 ± 0.106
1.625LeuCys: 1.625 ± 0.059
4.8LeuAsp: 4.8 ± 0.09
5.474LeuGlu: 5.474 ± 0.117
3.667LeuPhe: 3.667 ± 0.095
5.146LeuGly: 5.146 ± 0.13
1.205LeuHis: 1.205 ± 0.045
6.924LeuIle: 6.924 ± 0.117
6.684LeuLys: 6.684 ± 0.107
7.004LeuLeu: 7.004 ± 0.136
2.386LeuMet: 2.386 ± 0.075
4.604LeuAsn: 4.604 ± 0.087
3.102LeuPro: 3.102 ± 0.077
1.901LeuGln: 1.901 ± 0.062
3.128LeuArg: 3.128 ± 0.085
6.822LeuSer: 6.822 ± 0.117
5.026LeuThr: 5.026 ± 0.094
4.286LeuVal: 4.286 ± 0.093
0.509LeuTrp: 0.509 ± 0.029
3.086LeuTyr: 3.086 ± 0.084
0.003LeuXaa: 0.003 ± 0.002
Met
1.914MetAla: 1.914 ± 0.06
0.413MetCys: 0.413 ± 0.033
1.415MetAsp: 1.415 ± 0.048
1.716MetGlu: 1.716 ± 0.056
1.043MetPhe: 1.043 ± 0.043
1.735MetGly: 1.735 ± 0.067
0.451MetHis: 0.451 ± 0.028
2.206MetIle: 2.206 ± 0.061
2.386MetLys: 2.386 ± 0.06
2.788MetLeu: 2.788 ± 0.075
0.703MetMet: 0.703 ± 0.036
1.748MetAsn: 1.748 ± 0.056
1.135MetPro: 1.135 ± 0.045
0.854MetGln: 0.854 ± 0.044
1.036MetArg: 1.036 ± 0.047
1.943MetSer: 1.943 ± 0.057
1.524MetThr: 1.524 ± 0.047
1.38MetVal: 1.38 ± 0.049
0.159MetTrp: 0.159 ± 0.016
0.99MetTyr: 0.99 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
3.936AsnAla: 3.936 ± 0.07
1.03AsnCys: 1.03 ± 0.048
3.315AsnAsp: 3.315 ± 0.086
3.988AsnGlu: 3.988 ± 0.095
2.068AsnPhe: 2.068 ± 0.073
4.295AsnGly: 4.295 ± 0.093
0.719AsnHis: 0.719 ± 0.035
5.01AsnIle: 5.01 ± 0.091
4.067AsnLys: 4.067 ± 0.083
4.057AsnLeu: 4.057 ± 0.081
1.433AsnMet: 1.433 ± 0.049
3.275AsnAsn: 3.275 ± 0.091
2.173AsnPro: 2.173 ± 0.072
1.382AsnGln: 1.382 ± 0.051
1.856AsnArg: 1.856 ± 0.057
4.009AsnSer: 4.009 ± 0.087
2.893AsnThr: 2.893 ± 0.076
3.355AsnVal: 3.355 ± 0.088
0.357AsnTrp: 0.357 ± 0.027
2.241AsnTyr: 2.241 ± 0.063
0.003AsnXaa: 0.003 ± 0.002
Pro
2.05ProAla: 2.05 ± 0.067
0.486ProCys: 0.486 ± 0.035
2.397ProAsp: 2.397 ± 0.061
2.886ProGlu: 2.886 ± 0.076
1.454ProPhe: 1.454 ± 0.049
1.305ProGly: 1.305 ± 0.047
0.556ProHis: 0.556 ± 0.031
2.253ProIle: 2.253 ± 0.069
2.008ProLys: 2.008 ± 0.064
2.461ProLeu: 2.461 ± 0.066
0.771ProMet: 0.771 ± 0.035
1.473ProAsn: 1.473 ± 0.054
0.763ProPro: 0.763 ± 0.039
1.046ProGln: 1.046 ± 0.048
0.773ProArg: 0.773 ± 0.042
1.991ProSer: 1.991 ± 0.068
1.545ProThr: 1.545 ± 0.057
2.673ProVal: 2.673 ± 0.071
0.173ProTrp: 0.173 ± 0.015
1.294ProTyr: 1.294 ± 0.044
0.005ProXaa: 0.005 ± 0.003
Gln
1.699GlnAla: 1.699 ± 0.064
0.313GlnCys: 0.313 ± 0.021
1.093GlnAsp: 1.093 ± 0.044
1.571GlnGlu: 1.571 ± 0.051
1.067GlnPhe: 1.067 ± 0.043
1.499GlnGly: 1.499 ± 0.054
0.415GlnHis: 0.415 ± 0.025
2.194GlnIle: 2.194 ± 0.063
2.16GlnLys: 2.16 ± 0.067
2.566GlnLeu: 2.566 ± 0.071
0.747GlnMet: 0.747 ± 0.038
1.587GlnAsn: 1.587 ± 0.055
0.763GlnPro: 0.763 ± 0.038
0.994GlnGln: 0.994 ± 0.054
1.12GlnArg: 1.12 ± 0.043
1.681GlnSer: 1.681 ± 0.054
1.385GlnThr: 1.385 ± 0.043
1.294GlnVal: 1.294 ± 0.043
0.245GlnTrp: 0.245 ± 0.024
1.174GlnTyr: 1.174 ± 0.044
0.005GlnXaa: 0.005 ± 0.003
Arg
2.08ArgAla: 2.08 ± 0.065
0.626ArgCys: 0.626 ± 0.034
1.889ArgAsp: 1.889 ± 0.052
2.655ArgGlu: 2.655 ± 0.071
1.662ArgPhe: 1.662 ± 0.052
1.872ArgGly: 1.872 ± 0.062
0.696ArgHis: 0.696 ± 0.032
3.378ArgIle: 3.378 ± 0.076
3.152ArgLys: 3.152 ± 0.077
3.453ArgLeu: 3.453 ± 0.079
1.097ArgMet: 1.097 ± 0.045
2.169ArgAsn: 2.169 ± 0.066
0.99ArgPro: 0.99 ± 0.045
1.151ArgGln: 1.151 ± 0.047
1.814ArgArg: 1.814 ± 0.062
2.167ArgSer: 2.167 ± 0.065
1.954ArgThr: 1.954 ± 0.057
2.08ArgVal: 2.08 ± 0.059
0.233ArgTrp: 0.233 ± 0.023
1.567ArgTyr: 1.567 ± 0.051
0.002ArgXaa: 0.002 ± 0.002
Ser
5.374SerAla: 5.374 ± 0.098
1.163SerCys: 1.163 ± 0.05
4.952SerAsp: 4.952 ± 0.108
5.628SerGlu: 5.628 ± 0.113
3.271SerPhe: 3.271 ± 0.082
5.65SerGly: 5.65 ± 0.114
1.015SerHis: 1.015 ± 0.045
5.404SerIle: 5.404 ± 0.122
4.657SerLys: 4.657 ± 0.103
5.69SerLeu: 5.69 ± 0.113
1.847SerMet: 1.847 ± 0.07
3.114SerAsn: 3.114 ± 0.08
2.02SerPro: 2.02 ± 0.057
1.894SerGln: 1.894 ± 0.06
2.456SerArg: 2.456 ± 0.075
5.167SerSer: 5.167 ± 0.122
3.311SerThr: 3.311 ± 0.096
5.108SerVal: 5.108 ± 0.107
0.471SerTrp: 0.471 ± 0.023
2.757SerTyr: 2.757 ± 0.077
0.009SerXaa: 0.009 ± 0.004
Thr
4.935ThrAla: 4.935 ± 0.102
0.682ThrCys: 0.682 ± 0.034
3.595ThrAsp: 3.595 ± 0.076
4.113ThrGlu: 4.113 ± 0.098
2.223ThrPhe: 2.223 ± 0.058
4.09ThrGly: 4.09 ± 0.087
0.777ThrHis: 0.777 ± 0.037
4.202ThrIle: 4.202 ± 0.093
3.103ThrLys: 3.103 ± 0.079
4.445ThrLeu: 4.445 ± 0.083
1.169ThrMet: 1.169 ± 0.046
2.274ThrAsn: 2.274 ± 0.066
1.985ThrPro: 1.985 ± 0.065
1.399ThrGln: 1.399 ± 0.052
1.522ThrArg: 1.522 ± 0.058
3.593ThrSer: 3.593 ± 0.081
3.241ThrThr: 3.241 ± 0.277
4.524ThrVal: 4.524 ± 0.115
0.346ThrTrp: 0.346 ± 0.027
1.901ThrTyr: 1.901 ± 0.065
0.009ThrXaa: 0.009 ± 0.004
Val
3.614ValAla: 3.614 ± 0.097
1.259ValCys: 1.259 ± 0.048
3.64ValAsp: 3.64 ± 0.093
4.027ValGlu: 4.027 ± 0.084
2.963ValPhe: 2.963 ± 0.075
3.476ValGly: 3.476 ± 0.088
1.022ValHis: 1.022 ± 0.044
6.0ValIle: 6.0 ± 0.115
4.868ValLys: 4.868 ± 0.097
5.397ValLeu: 5.397 ± 0.101
1.903ValMet: 1.903 ± 0.065
3.513ValAsn: 3.513 ± 0.079
2.265ValPro: 2.265 ± 0.066
1.557ValGln: 1.557 ± 0.052
2.358ValArg: 2.358 ± 0.065
5.185ValSer: 5.185 ± 0.108
3.85ValThr: 3.85 ± 0.089
3.833ValVal: 3.833 ± 0.09
0.481ValTrp: 0.481 ± 0.029
2.68ValTyr: 2.68 ± 0.071
0.01ValXaa: 0.01 ± 0.004
Trp
0.381TrpAla: 0.381 ± 0.03
0.15TrpCys: 0.15 ± 0.014
0.434TrpAsp: 0.434 ± 0.03
0.408TrpGlu: 0.408 ± 0.029
0.315TrpPhe: 0.315 ± 0.027
0.497TrpGly: 0.497 ± 0.034
0.159TrpHis: 0.159 ± 0.018
0.562TrpIle: 0.562 ± 0.033
0.488TrpLys: 0.488 ± 0.027
0.614TrpLeu: 0.614 ± 0.036
0.15TrpMet: 0.15 ± 0.014
0.448TrpAsn: 0.448 ± 0.03
0.126TrpPro: 0.126 ± 0.016
0.273TrpGln: 0.273 ± 0.025
0.226TrpArg: 0.226 ± 0.02
0.436TrpSer: 0.436 ± 0.031
0.383TrpThr: 0.383 ± 0.026
0.303TrpVal: 0.303 ± 0.024
0.089TrpTrp: 0.089 ± 0.013
0.352TrpTyr: 0.352 ± 0.026
0.002TrpXaa: 0.002 ± 0.002
Tyr
2.675TyrAla: 2.675 ± 0.072
0.831TyrCys: 0.831 ± 0.04
2.844TyrAsp: 2.844 ± 0.086
2.682TyrGlu: 2.682 ± 0.07
1.887TyrPhe: 1.887 ± 0.055
2.904TyrGly: 2.904 ± 0.071
0.661TyrHis: 0.661 ± 0.031
3.661TyrIle: 3.661 ± 0.089
2.792TyrLys: 2.792 ± 0.075
3.173TyrLeu: 3.173 ± 0.078
0.988TyrMet: 0.988 ± 0.046
2.519TyrAsn: 2.519 ± 0.075
1.422TyrPro: 1.422 ± 0.052
0.98TyrGln: 0.98 ± 0.044
1.599TyrArg: 1.599 ± 0.057
3.154TyrSer: 3.154 ± 0.086
2.372TyrThr: 2.372 ± 0.07
2.397TyrVal: 2.397 ± 0.065
0.292TyrTrp: 0.292 ± 0.023
1.978TyrTyr: 1.978 ± 0.066
0.005TyrXaa: 0.005 ± 0.003
Xaa
0.005XaaAla: 0.005 ± 0.003
0.003XaaCys: 0.003 ± 0.003
0.003XaaAsp: 0.003 ± 0.003
0.002XaaGlu: 0.002 ± 0.002
0.002XaaPhe: 0.002 ± 0.002
0.01XaaGly: 0.01 ± 0.004
0.005XaaHis: 0.005 ± 0.003
0.007XaaIle: 0.007 ± 0.004
0.002XaaLys: 0.002 ± 0.002
0.017XaaLeu: 0.017 ± 0.005
0.003XaaMet: 0.003 ± 0.002
0.005XaaAsn: 0.005 ± 0.003
0.003XaaPro: 0.003 ± 0.002
0.005XaaGln: 0.005 ± 0.003
0.01XaaArg: 0.01 ± 0.004
0.005XaaSer: 0.005 ± 0.003
0.0XaaThr: 0.0 ± 0.0
0.002XaaVal: 0.002 ± 0.002
0.0XaaTrp: 0.0 ± 0.0
0.002XaaTyr: 0.002 ± 0.002
0.114XaaXaa: 0.114 ± 0.019
Statistics based on 1963 proteins (571658 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski