Hubei lepidoptera virus 3
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KNZ0|A0A1L3KNZ0_9VIRU Cypovirus VP8 OS=Hubei lepidoptera virus 3 OX=1922905 PE=4 SV=1
MM1 pKa = 7.41 LTFQVTFKK9 pKa = 10.87 DD10 pKa = 3.83 VKK12 pKa = 10.43 TNDD15 pKa = 3.16 LKK17 pKa = 11.17 KK18 pKa = 10.71 INIHH22 pKa = 4.61 TAKK25 pKa = 9.72 PQKK28 pKa = 10.72 DD29 pKa = 3.32 YY30 pKa = 11.81 DD31 pKa = 3.84 KK32 pKa = 11.45 FHH34 pKa = 7.89 DD35 pKa = 4.44 KK36 pKa = 10.45 LAKK39 pKa = 9.84 KK40 pKa = 10.21 NRR42 pKa = 11.84 EE43 pKa = 3.79 LTQNAKK49 pKa = 9.26 MSIMPRR55 pKa = 11.84 VVTDD59 pKa = 3.63 KK60 pKa = 10.32 VTADD64 pKa = 4.98 SIIQKK69 pKa = 10.34 NKK71 pKa = 9.71 IEE73 pKa = 4.16 TEE75 pKa = 3.89 VLYY78 pKa = 10.53 ISMRR82 pKa = 11.84 APRR85 pKa = 11.84 IDD87 pKa = 3.75 PDD89 pKa = 4.77 AIVCANEE96 pKa = 3.86 LGLPILSRR104 pKa = 11.84 VGVEE108 pKa = 3.45 EE109 pKa = 4.53 TMFLKK114 pKa = 10.51 DD115 pKa = 3.3 KK116 pKa = 10.43 RR117 pKa = 11.84 YY118 pKa = 9.3 QYY120 pKa = 10.98 VSKK123 pKa = 11.22 EE124 pKa = 3.99 EE125 pKa = 3.95 VDD127 pKa = 3.37 VDD129 pKa = 4.7 FFDD132 pKa = 4.32 EE133 pKa = 4.05 LTMAINRR140 pKa = 11.84 KK141 pKa = 8.95 IGKK144 pKa = 9.69 KK145 pKa = 9.87 SGSDD149 pKa = 3.35 EE150 pKa = 3.73 GTMIKK155 pKa = 9.31 MVKK158 pKa = 10.01 NDD160 pKa = 4.13 LKK162 pKa = 9.73 SASWIMEE169 pKa = 4.08 NNVKK173 pKa = 10.62 LFVRR177 pKa = 11.84 TGLNKK182 pKa = 10.41 KK183 pKa = 10.38 LDD185 pKa = 4.24 DD186 pKa = 4.36 LCVKK190 pKa = 10.35 PSDD193 pKa = 3.31 IGNVRR198 pKa = 11.84 EE199 pKa = 3.8 IRR201 pKa = 11.84 AVDD204 pKa = 3.87 EE205 pKa = 4.29 RR206 pKa = 11.84 WHH208 pKa = 7.45 GEE210 pKa = 4.09 DD211 pKa = 3.33 EE212 pKa = 4.76 GVTLTYY218 pKa = 10.48 SIYY221 pKa = 11.16 SNDD224 pKa = 3.19 FNEE227 pKa = 5.4 LIRR230 pKa = 11.84 TQEE233 pKa = 3.22 IDD235 pKa = 3.03 KK236 pKa = 10.82 AIRR239 pKa = 11.84 DD240 pKa = 4.02 VKK242 pKa = 10.26 EE243 pKa = 4.31 HH244 pKa = 7.18 IEE246 pKa = 4.31 DD247 pKa = 3.46 EE248 pKa = 4.17 HH249 pKa = 8.4 HH250 pKa = 5.75 GAKK253 pKa = 9.52 GRR255 pKa = 11.84 KK256 pKa = 9.07 VYY258 pKa = 10.49 IIYY261 pKa = 10.65 DD262 pKa = 3.8 GEE264 pKa = 4.56 DD265 pKa = 3.41 SNKK268 pKa = 9.62 MKK270 pKa = 10.58 VKK272 pKa = 10.63 KK273 pKa = 10.24 FINEE277 pKa = 4.0 LSYY280 pKa = 10.99 EE281 pKa = 3.77 IDD283 pKa = 3.68 GQVVEE288 pKa = 4.28 YY289 pKa = 10.32 RR290 pKa = 11.84 VNEE293 pKa = 4.13 KK294 pKa = 10.99 NDD296 pKa = 3.32 VKK298 pKa = 11.18 YY299 pKa = 11.19 SLIQTCRR306 pKa = 11.84 IAEE309 pKa = 4.19 SEE311 pKa = 4.64 LEE313 pKa = 4.22 DD314 pKa = 3.55 EE315 pKa = 4.48 TMKK318 pKa = 11.33 DD319 pKa = 3.23 NVIFEE324 pKa = 4.56 GKK326 pKa = 8.44 RR327 pKa = 11.84 TEE329 pKa = 4.03 LKK331 pKa = 10.57 EE332 pKa = 4.06 IILNEE337 pKa = 3.8 IEE339 pKa = 4.01 PLYY342 pKa = 10.79 RR343 pKa = 11.84 KK344 pKa = 9.97 APEE347 pKa = 4.11 SGRR350 pKa = 11.84 EE351 pKa = 4.28 DD352 pKa = 3.5 IDD354 pKa = 4.27 KK355 pKa = 10.6 MKK357 pKa = 10.99 ALLNSEE363 pKa = 5.1 RR364 pKa = 11.84 YY365 pKa = 10.25 VNDD368 pKa = 3.15 TDD370 pKa = 3.94 VIKK373 pKa = 10.92 VEE375 pKa = 5.0 RR376 pKa = 11.84 IPLPSKK382 pKa = 10.44 KK383 pKa = 9.95 PEE385 pKa = 3.51 IAEE388 pKa = 4.22 NVNKK392 pKa = 9.11 MRR394 pKa = 11.84 KK395 pKa = 9.7 DD396 pKa = 3.19 ILVKK400 pKa = 10.34 IGKK403 pKa = 9.63 CDD405 pKa = 3.73 DD406 pKa = 4.23 AKK408 pKa = 11.18 DD409 pKa = 3.58 GAIIMSLEE417 pKa = 3.96 RR418 pKa = 11.84 EE419 pKa = 4.11 LGNNIWRR426 pKa = 11.84 GEE428 pKa = 4.18 EE429 pKa = 3.75 TWQSRR434 pKa = 11.84 MQKK437 pKa = 9.69 RR438 pKa = 11.84 FPEE441 pKa = 4.14 TSVFYY446 pKa = 10.27 AYY448 pKa = 10.48 SDD450 pKa = 3.48 RR451 pKa = 11.84 VGTGDD456 pKa = 4.57 GIKK459 pKa = 9.41 IDD461 pKa = 3.7 KK462 pKa = 10.35 AIKK465 pKa = 10.45 NKK467 pKa = 8.68 MSKK470 pKa = 10.04 PKK472 pKa = 10.24 SVYY475 pKa = 10.36 NKK477 pKa = 10.12 EE478 pKa = 4.25 DD479 pKa = 3.42 LEE481 pKa = 4.47 KK482 pKa = 11.3 YY483 pKa = 9.7 EE484 pKa = 5.02 IIGIEE489 pKa = 3.94 PNYY492 pKa = 10.35 VEE494 pKa = 4.54 SLEE497 pKa = 4.39 EE498 pKa = 3.73 EE499 pKa = 4.11 LDD501 pKa = 3.57 RR502 pKa = 11.84 RR503 pKa = 11.84 NVDD506 pKa = 3.31 LEE508 pKa = 3.94 LRR510 pKa = 11.84 KK511 pKa = 10.13 LISAYY516 pKa = 10.63 SSAKK520 pKa = 9.94 NRR522 pKa = 11.84 MILTEE527 pKa = 4.63 MIACEE532 pKa = 4.13 NYY534 pKa = 10.43 NGNTFIKK541 pKa = 10.33 FIEE544 pKa = 4.05 QMIAASVADD553 pKa = 3.74 KK554 pKa = 10.83 GITMFVEE561 pKa = 4.47 DD562 pKa = 4.73 EE563 pKa = 4.38 SFKK566 pKa = 11.03 TMADD570 pKa = 3.06 KK571 pKa = 11.02 VINKK575 pKa = 8.62 IRR577 pKa = 11.84 NEE579 pKa = 3.73 NMEE582 pKa = 3.75 IRR584 pKa = 11.84 EE585 pKa = 4.19 VYY587 pKa = 10.35 QLFITEE593 pKa = 4.49 KK594 pKa = 10.05 YY595 pKa = 9.83 INARR599 pKa = 11.84 DD600 pKa = 3.73 FLGEE604 pKa = 4.05 SGDD607 pKa = 3.71 IASEE611 pKa = 4.03 VAEE614 pKa = 4.76 DD615 pKa = 4.42 KK616 pKa = 10.84 KK617 pKa = 10.95 DD618 pKa = 3.52 SQIFEE623 pKa = 4.26 EE624 pKa = 4.63 EE625 pKa = 4.37 GNDD628 pKa = 3.37 EE629 pKa = 4.32 YY630 pKa = 11.41 EE631 pKa = 4.14 EE632 pKa = 3.92 EE633 pKa = 4.25 RR634 pKa = 11.84 NLVKK638 pKa = 10.47 EE639 pKa = 4.2 DD640 pKa = 3.6 NGNEE644 pKa = 4.04 EE645 pKa = 4.31 LSDD648 pKa = 3.6 VRR650 pKa = 11.84 DD651 pKa = 3.46 KK652 pKa = 11.54 KK653 pKa = 10.74 EE654 pKa = 3.89 EE655 pKa = 4.03 KK656 pKa = 10.35 EE657 pKa = 3.9 EE658 pKa = 4.57 SNGGKK663 pKa = 9.25 VRR665 pKa = 11.84 YY666 pKa = 9.26 EE667 pKa = 3.79 SVIVNEE673 pKa = 4.6 KK674 pKa = 10.73 NDD676 pKa = 3.65 DD677 pKa = 3.78 KK678 pKa = 11.51 NFVVIDD684 pKa = 3.78 VYY686 pKa = 11.43 EE687 pKa = 4.31 EE688 pKa = 4.21 GKK690 pKa = 11.18 KK691 pKa = 9.51 MLTDD695 pKa = 3.84 AFNRR699 pKa = 11.84 SFEE702 pKa = 4.26 EE703 pKa = 3.81 LRR705 pKa = 11.84 EE706 pKa = 4.26 AKK708 pKa = 9.5 TKK710 pKa = 9.62 EE711 pKa = 3.58 QIEE714 pKa = 4.4 IIKK717 pKa = 10.15 LKK719 pKa = 10.79 SEE721 pKa = 4.37 TYY723 pKa = 10.05 VEE725 pKa = 4.31 LFKK728 pKa = 11.15 IMNRR732 pKa = 11.84 GMGEE736 pKa = 3.75 LEE738 pKa = 4.6 EE739 pKa = 4.06 IRR741 pKa = 11.84 KK742 pKa = 9.75 EE743 pKa = 4.03 NVNKK747 pKa = 9.96 VEE749 pKa = 4.08 RR750 pKa = 11.84 VEE752 pKa = 4.8 EE753 pKa = 3.94 FDD755 pKa = 5.54 KK756 pKa = 11.57 GDD758 pKa = 3.45 NTDD761 pKa = 3.28 NRR763 pKa = 11.84 VVEE766 pKa = 4.35 MKK768 pKa = 10.41 EE769 pKa = 3.95 KK770 pKa = 10.66 VVNALMLTHH779 pKa = 7.02 IDD781 pKa = 3.55 FMEE784 pKa = 4.33 KK785 pKa = 10.27 VEE787 pKa = 4.03 MDD789 pKa = 3.7 EE790 pKa = 4.97 RR791 pKa = 11.84 PIIMVARR798 pKa = 11.84 SEE800 pKa = 4.34 KK801 pKa = 9.66 YY802 pKa = 9.8 SGYY805 pKa = 5.45 EE806 pKa = 3.71 TKK808 pKa = 10.16 MKK810 pKa = 9.73 GTIRR814 pKa = 11.84 YY815 pKa = 9.9 GEE817 pKa = 4.24 TNDD820 pKa = 3.06 RR821 pKa = 11.84 KK822 pKa = 10.81 YY823 pKa = 11.44 NVFEE827 pKa = 4.67 SNTIKK832 pKa = 10.67 KK833 pKa = 9.84 VIQDD837 pKa = 3.49 EE838 pKa = 4.32 MRR840 pKa = 11.84 SGGNEE845 pKa = 3.65 RR846 pKa = 11.84 ATTEE850 pKa = 3.91 AVLNMLKK857 pKa = 10.13 ISLHH861 pKa = 4.92 KK862 pKa = 10.04 RR863 pKa = 11.84 GYY865 pKa = 8.96 VVEE868 pKa = 4.31 TNEE871 pKa = 4.21 EE872 pKa = 3.52 GDD874 pKa = 3.65 IIIRR878 pKa = 11.84 KK879 pKa = 7.87 YY880 pKa = 10.78
Molecular weight: 102.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.138
IPC2_protein 5.143
IPC_protein 5.08
Toseland 4.965
ProMoST 5.169
Dawson 5.016
Bjellqvist 5.169
Wikipedia 4.863
Rodwell 4.94
Grimsley 4.876
Solomon 5.016
Lehninger 4.978
Nozaki 5.13
DTASelect 5.245
Thurlkill 4.952
EMBOSS 4.889
Sillero 5.207
Patrickios 4.431
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.206
Protein with the highest isoelectric point:
>tr|A0A1L3KP70|A0A1L3KP70_9VIRU Cypovirus VP2 OS=Hubei lepidoptera virus 3 OX=1922905 PE=4 SV=1
MM1 pKa = 7.72 EE2 pKa = 5.08 KK3 pKa = 10.3 RR4 pKa = 11.84 VINKK8 pKa = 9.26 NVINKK13 pKa = 9.3 EE14 pKa = 3.63 KK15 pKa = 10.46 EE16 pKa = 3.7 INKK19 pKa = 9.79 KK20 pKa = 10.11 YY21 pKa = 10.2 KK22 pKa = 9.71 PKK24 pKa = 10.62 NRR26 pKa = 11.84 LNVIWTVNLKK36 pKa = 10.37 DD37 pKa = 3.9 KK38 pKa = 11.09 NVLRR42 pKa = 11.84 ILSEE46 pKa = 4.18 LGVEE50 pKa = 4.86 IYY52 pKa = 10.37 RR53 pKa = 11.84 QEE55 pKa = 4.2 GEE57 pKa = 4.18 KK58 pKa = 10.77 KK59 pKa = 9.04 SDD61 pKa = 2.76 RR62 pKa = 11.84 RR63 pKa = 11.84 ARR65 pKa = 11.84 AICANEE71 pKa = 3.95 QIEE74 pKa = 4.49 WASDD78 pKa = 2.88 IHH80 pKa = 6.45 GWLDD84 pKa = 3.63 KK85 pKa = 10.93 LSKK88 pKa = 10.63 RR89 pKa = 11.84 NNEE92 pKa = 3.67 NLMNLGVISKK102 pKa = 9.98 ILRR105 pKa = 11.84 NEE107 pKa = 3.91 VIEE110 pKa = 4.29 YY111 pKa = 10.04 NVSEE115 pKa = 4.25 GQLKK119 pKa = 10.23 RR120 pKa = 11.84 EE121 pKa = 3.98 EE122 pKa = 4.22 TKK124 pKa = 10.52 EE125 pKa = 3.8 IEE127 pKa = 4.33 VEE129 pKa = 4.19 SEE131 pKa = 4.06 GLLKK135 pKa = 10.77 YY136 pKa = 10.63 NRR138 pKa = 11.84 ADD140 pKa = 3.4 NDD142 pKa = 3.41 NVMKK146 pKa = 9.44 LTKK149 pKa = 10.55 EE150 pKa = 3.9 EE151 pKa = 4.11 IEE153 pKa = 4.41 CLKK156 pKa = 9.89 EE157 pKa = 4.0 TIKK160 pKa = 10.15 TGLHH164 pKa = 5.55 SEE166 pKa = 4.32 GAAFKK171 pKa = 9.94 MIEE174 pKa = 4.02 IKK176 pKa = 10.48 IPALMRR182 pKa = 11.84 LTPKK186 pKa = 10.57 YY187 pKa = 10.06 INQLRR192 pKa = 11.84 ARR194 pKa = 11.84 VNVEE198 pKa = 3.83 QISVTEE204 pKa = 4.87 IIHH207 pKa = 6.15 SDD209 pKa = 2.85 NYY211 pKa = 7.11 YY212 pKa = 11.16 ARR214 pKa = 11.84 MIFEE218 pKa = 4.83 KK219 pKa = 10.66 PLLIYY224 pKa = 10.47 NAISMNVIRR233 pKa = 11.84 KK234 pKa = 9.26 LKK236 pKa = 10.56 ADD238 pKa = 3.77 DD239 pKa = 3.56 RR240 pKa = 11.84 QIYY243 pKa = 9.82 IMSGEE248 pKa = 4.21 VNHH251 pKa = 7.27 KK252 pKa = 10.62 DD253 pKa = 3.39 MRR255 pKa = 11.84 DD256 pKa = 3.06 KK257 pKa = 11.14 GLRR260 pKa = 11.84 VYY262 pKa = 10.55 VPQASKK268 pKa = 11.26 LIFTMKK274 pKa = 9.99 NRR276 pKa = 11.84 KK277 pKa = 8.99 LSIYY281 pKa = 10.43 VGLPPQMFQNRR292 pKa = 11.84 IDD294 pKa = 3.63 EE295 pKa = 4.71 RR296 pKa = 11.84 YY297 pKa = 10.02 HH298 pKa = 6.53 NFTNEE303 pKa = 3.52 IADD306 pKa = 3.34 ITMIPSMQSTFGMGMSKK323 pKa = 9.01 LTRR326 pKa = 11.84 VMAATPSTPRR336 pKa = 11.84 LTSKK340 pKa = 9.91 ATLEE344 pKa = 3.99 KK345 pKa = 10.71 LKK347 pKa = 10.45 MEE349 pKa = 4.43 IASDD353 pKa = 3.76 RR354 pKa = 11.84 KK355 pKa = 9.83 VVTCIVIGNKK365 pKa = 10.22 GSGKK369 pKa = 6.85 TTMTKK374 pKa = 10.72 KK375 pKa = 9.8 MVEE378 pKa = 4.53 LLDD381 pKa = 3.73 GMNGRR386 pKa = 11.84 RR387 pKa = 11.84 CYY389 pKa = 10.53 RR390 pKa = 11.84 IDD392 pKa = 3.18 SDD394 pKa = 4.99 APGRR398 pKa = 11.84 WMYY401 pKa = 11.56 DD402 pKa = 2.79 RR403 pKa = 11.84 ANKK406 pKa = 8.81 VVCTSFDD413 pKa = 3.25 EE414 pKa = 4.39 VLSYY418 pKa = 11.06 NSEE421 pKa = 4.3 MYY423 pKa = 10.2 ISLYY427 pKa = 10.87 EE428 pKa = 4.09 KK429 pKa = 10.22 MVDD432 pKa = 5.09 DD433 pKa = 4.14 MMQAEE438 pKa = 4.83 KK439 pKa = 11.12 LDD441 pKa = 3.51 VDD443 pKa = 3.77 KK444 pKa = 11.01 FNRR447 pKa = 11.84 MSNAKK452 pKa = 9.32 KK453 pKa = 10.67 NEE455 pKa = 4.04 MIDD458 pKa = 3.26 ALKK461 pKa = 11.0 RR462 pKa = 11.84 MIDD465 pKa = 4.14 DD466 pKa = 3.85 KK467 pKa = 11.46 LISNKK472 pKa = 10.31 EE473 pKa = 3.98 EE474 pKa = 3.89 VNEE477 pKa = 3.99 KK478 pKa = 10.19 QFYY481 pKa = 11.18 DD482 pKa = 3.72 MVHH485 pKa = 6.19 EE486 pKa = 4.14 LVPVNSTLIIEE497 pKa = 4.54 AHH499 pKa = 7.4 RR500 pKa = 11.84 ITQDD504 pKa = 2.94 AVLGGTDD511 pKa = 2.86 ISMLYY516 pKa = 10.41 EE517 pKa = 4.68 GVQDD521 pKa = 3.79 PLIAFMRR528 pKa = 11.84 RR529 pKa = 11.84 GALLRR534 pKa = 11.84 DD535 pKa = 4.31 LILRR539 pKa = 11.84 EE540 pKa = 4.49 IYY542 pKa = 10.45 QKK544 pKa = 10.73 DD545 pKa = 3.63 YY546 pKa = 11.07 SYY548 pKa = 11.56 SHH550 pKa = 6.88 VMINAMEE557 pKa = 4.16 MGVVLSEE564 pKa = 4.07
Molecular weight: 65.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.291
IPC2_protein 8.273
IPC_protein 8.156
Toseland 8.843
ProMoST 8.697
Dawson 9.165
Bjellqvist 8.975
Wikipedia 9.311
Rodwell 9.385
Grimsley 9.18
Solomon 9.224
Lehninger 9.18
Nozaki 9.092
DTASelect 8.902
Thurlkill 9.048
EMBOSS 9.311
Sillero 9.194
Patrickios 4.774
IPC_peptide 9.209
IPC2_peptide 7.527
IPC2.peptide.svr19 7.621
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
7784
253
1335
778.4
89.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.959 ± 0.327
0.732 ± 0.23
6.115 ± 0.249
8.941 ± 0.695
3.25 ± 0.2
5.588 ± 0.395
1.375 ± 0.11
8.363 ± 0.338
7.027 ± 0.878
7.798 ± 0.363
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.188 ± 0.218
6.385 ± 0.336
2.595 ± 0.198
2.762 ± 0.287
6.655 ± 0.258
6.166 ± 0.365
5.383 ± 0.393
6.706 ± 0.361
0.797 ± 0.086
4.214 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here