Amino acid dipepetide frequency for Mycobacterium phage ACFishhook

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.435AlaAla: 10.435 ± 0.993
0.622AlaCys: 0.622 ± 0.209
4.976AlaAsp: 4.976 ± 0.692
7.187AlaGlu: 7.187 ± 0.854
3.455AlaPhe: 3.455 ± 0.479
7.049AlaGly: 7.049 ± 0.799
1.797AlaHis: 1.797 ± 0.381
4.423AlaIle: 4.423 ± 0.606
4.285AlaLys: 4.285 ± 0.615
9.537AlaLeu: 9.537 ± 1.23
2.695AlaMet: 2.695 ± 0.478
2.972AlaAsn: 2.972 ± 0.56
4.63AlaPro: 4.63 ± 0.82
4.492AlaGln: 4.492 ± 0.662
6.427AlaArg: 6.427 ± 0.628
5.46AlaSer: 5.46 ± 0.649
6.911AlaThr: 6.911 ± 0.624
6.496AlaVal: 6.496 ± 0.789
2.142AlaTrp: 2.142 ± 0.377
2.419AlaTyr: 2.419 ± 0.423
0.0AlaXaa: 0.0 ± 0.0
Cys
0.553CysAla: 0.553 ± 0.194
0.0CysCys: 0.0 ± 0.0
0.415CysAsp: 0.415 ± 0.19
0.484CysGlu: 0.484 ± 0.164
0.069CysPhe: 0.069 ± 0.06
0.484CysGly: 0.484 ± 0.158
0.207CysHis: 0.207 ± 0.112
0.138CysIle: 0.138 ± 0.09
0.276CysLys: 0.276 ± 0.128
0.415CysLeu: 0.415 ± 0.161
0.138CysMet: 0.138 ± 0.084
0.622CysAsn: 0.622 ± 0.22
0.346CysPro: 0.346 ± 0.162
0.138CysGln: 0.138 ± 0.094
0.553CysArg: 0.553 ± 0.163
0.276CysSer: 0.276 ± 0.14
0.415CysThr: 0.415 ± 0.225
0.622CysVal: 0.622 ± 0.211
0.069CysTrp: 0.069 ± 0.077
0.415CysTyr: 0.415 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
6.911AspAla: 6.911 ± 0.868
0.691AspCys: 0.691 ± 0.177
3.732AspAsp: 3.732 ± 0.604
3.801AspGlu: 3.801 ± 0.573
2.211AspPhe: 2.211 ± 0.348
5.045AspGly: 5.045 ± 0.565
1.52AspHis: 1.52 ± 0.419
2.557AspIle: 2.557 ± 0.405
2.695AspLys: 2.695 ± 0.811
5.39AspLeu: 5.39 ± 0.586
1.106AspMet: 1.106 ± 0.259
1.728AspAsn: 1.728 ± 0.39
5.321AspPro: 5.321 ± 0.765
1.728AspGln: 1.728 ± 0.358
3.455AspArg: 3.455 ± 0.561
3.594AspSer: 3.594 ± 0.443
3.801AspThr: 3.801 ± 0.605
3.939AspVal: 3.939 ± 0.447
1.244AspTrp: 1.244 ± 0.278
2.626AspTyr: 2.626 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
7.256GluAla: 7.256 ± 0.82
0.207GluCys: 0.207 ± 0.11
4.976GluAsp: 4.976 ± 0.623
5.39GluGlu: 5.39 ± 0.626
2.142GluPhe: 2.142 ± 0.329
4.907GluGly: 4.907 ± 0.659
1.313GluHis: 1.313 ± 0.294
3.317GluIle: 3.317 ± 0.49
2.35GluLys: 2.35 ± 0.408
6.289GluLeu: 6.289 ± 0.62
2.35GluMet: 2.35 ± 0.372
2.35GluAsn: 2.35 ± 0.376
2.972GluPro: 2.972 ± 0.503
2.903GluGln: 2.903 ± 0.341
4.838GluArg: 4.838 ± 0.624
2.764GluSer: 2.764 ± 0.462
3.455GluThr: 3.455 ± 0.462
4.354GluVal: 4.354 ± 0.553
1.244GluTrp: 1.244 ± 0.32
2.281GluTyr: 2.281 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
3.317PheAla: 3.317 ± 0.546
0.138PheCys: 0.138 ± 0.098
2.833PheAsp: 2.833 ± 0.511
2.004PheGlu: 2.004 ± 0.379
0.622PhePhe: 0.622 ± 0.243
3.525PheGly: 3.525 ± 0.584
0.553PheHis: 0.553 ± 0.216
1.728PheIle: 1.728 ± 0.331
1.175PheLys: 1.175 ± 0.313
3.179PheLeu: 3.179 ± 0.464
0.968PheMet: 0.968 ± 0.277
1.52PheAsn: 1.52 ± 0.342
1.797PhePro: 1.797 ± 0.384
1.175PheGln: 1.175 ± 0.301
1.797PheArg: 1.797 ± 0.366
2.004PheSer: 2.004 ± 0.368
2.35PheThr: 2.35 ± 0.364
2.073PheVal: 2.073 ± 0.446
0.484PheTrp: 0.484 ± 0.201
0.691PheTyr: 0.691 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
7.602GlyAla: 7.602 ± 0.937
0.622GlyCys: 0.622 ± 0.228
6.911GlyAsp: 6.911 ± 1.537
4.838GlyGlu: 4.838 ± 0.637
3.594GlyPhe: 3.594 ± 0.512
7.671GlyGly: 7.671 ± 1.378
1.797GlyHis: 1.797 ± 0.416
4.008GlyIle: 4.008 ± 0.543
3.041GlyLys: 3.041 ± 0.41
6.151GlyLeu: 6.151 ± 0.772
2.35GlyMet: 2.35 ± 0.423
3.525GlyAsn: 3.525 ± 0.602
6.496GlyPro: 6.496 ± 2.489
3.041GlyGln: 3.041 ± 0.448
4.147GlyArg: 4.147 ± 0.478
4.077GlySer: 4.077 ± 0.626
5.39GlyThr: 5.39 ± 0.614
6.151GlyVal: 6.151 ± 0.735
2.004GlyTrp: 2.004 ± 0.357
2.903GlyTyr: 2.903 ± 0.486
0.0GlyXaa: 0.0 ± 0.0
His
1.244HisAla: 1.244 ± 0.314
0.346HisCys: 0.346 ± 0.152
1.52HisAsp: 1.52 ± 0.347
1.52HisGlu: 1.52 ± 0.332
0.553HisPhe: 0.553 ± 0.199
2.004HisGly: 2.004 ± 0.488
0.622HisHis: 0.622 ± 0.195
0.968HisIle: 0.968 ± 0.178
0.622HisLys: 0.622 ± 0.225
1.589HisLeu: 1.589 ± 0.557
0.346HisMet: 0.346 ± 0.15
0.76HisAsn: 0.76 ± 0.209
1.037HisPro: 1.037 ± 0.273
0.968HisGln: 0.968 ± 0.284
1.866HisArg: 1.866 ± 0.427
0.553HisSer: 0.553 ± 0.202
1.037HisThr: 1.037 ± 0.268
1.313HisVal: 1.313 ± 0.284
0.415HisTrp: 0.415 ± 0.207
0.622HisTyr: 0.622 ± 0.192
0.0HisXaa: 0.0 ± 0.0
Ile
4.907IleAla: 4.907 ± 0.783
0.276IleCys: 0.276 ± 0.126
3.594IleAsp: 3.594 ± 0.391
3.386IleGlu: 3.386 ± 0.565
1.589IlePhe: 1.589 ± 0.319
4.147IleGly: 4.147 ± 0.636
0.898IleHis: 0.898 ± 0.257
1.313IleIle: 1.313 ± 0.347
1.797IleLys: 1.797 ± 0.343
3.248IleLeu: 3.248 ± 0.413
0.76IleMet: 0.76 ± 0.254
1.313IleAsn: 1.313 ± 0.357
3.594IlePro: 3.594 ± 0.442
1.866IleGln: 1.866 ± 0.448
2.903IleArg: 2.903 ± 0.409
2.419IleSer: 2.419 ± 0.447
3.11IleThr: 3.11 ± 0.529
3.455IleVal: 3.455 ± 0.438
1.106IleTrp: 1.106 ± 0.276
1.175IleTyr: 1.175 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
4.423LysAla: 4.423 ± 0.577
0.138LysCys: 0.138 ± 0.093
2.626LysAsp: 2.626 ± 0.474
2.557LysGlu: 2.557 ± 0.389
1.589LysPhe: 1.589 ± 0.254
4.423LysGly: 4.423 ± 1.475
1.106LysHis: 1.106 ± 0.301
2.281LysIle: 2.281 ± 0.411
2.488LysLys: 2.488 ± 0.462
3.317LysLeu: 3.317 ± 0.527
0.76LysMet: 0.76 ± 0.202
0.691LysAsn: 0.691 ± 0.213
2.211LysPro: 2.211 ± 0.446
2.281LysGln: 2.281 ± 0.398
3.317LysArg: 3.317 ± 0.657
1.935LysSer: 1.935 ± 0.402
2.695LysThr: 2.695 ± 0.36
2.972LysVal: 2.972 ± 0.477
0.829LysTrp: 0.829 ± 0.249
0.553LysTyr: 0.553 ± 0.154
0.0LysXaa: 0.0 ± 0.0
Leu
8.155LeuAla: 8.155 ± 1.123
0.207LeuCys: 0.207 ± 0.11
4.976LeuAsp: 4.976 ± 0.61
5.39LeuGlu: 5.39 ± 0.582
2.419LeuPhe: 2.419 ± 0.385
6.704LeuGly: 6.704 ± 0.542
1.797LeuHis: 1.797 ± 0.382
4.354LeuIle: 4.354 ± 0.478
4.077LeuLys: 4.077 ± 0.528
6.358LeuLeu: 6.358 ± 0.688
2.281LeuMet: 2.281 ± 0.43
2.833LeuAsn: 2.833 ± 0.522
4.285LeuPro: 4.285 ± 0.552
2.557LeuGln: 2.557 ± 0.635
6.911LeuArg: 6.911 ± 0.773
4.838LeuSer: 4.838 ± 0.566
5.114LeuThr: 5.114 ± 0.643
5.114LeuVal: 5.114 ± 0.705
1.175LeuTrp: 1.175 ± 0.276
2.626LeuTyr: 2.626 ± 0.426
0.0LeuXaa: 0.0 ± 0.0
Met
2.764MetAla: 2.764 ± 0.446
0.0MetCys: 0.0 ± 0.0
1.244MetAsp: 1.244 ± 0.383
1.244MetGlu: 1.244 ± 0.336
0.76MetPhe: 0.76 ± 0.257
2.004MetGly: 2.004 ± 0.306
0.276MetHis: 0.276 ± 0.13
1.728MetIle: 1.728 ± 0.327
1.451MetLys: 1.451 ± 0.37
1.797MetLeu: 1.797 ± 0.302
0.553MetMet: 0.553 ± 0.235
1.106MetAsn: 1.106 ± 0.25
1.935MetPro: 1.935 ± 0.432
0.76MetGln: 0.76 ± 0.378
2.004MetArg: 2.004 ± 0.309
1.797MetSer: 1.797 ± 0.331
2.073MetThr: 2.073 ± 0.28
1.659MetVal: 1.659 ± 0.332
0.138MetTrp: 0.138 ± 0.091
0.691MetTyr: 0.691 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
3.732AsnAla: 3.732 ± 0.621
0.207AsnCys: 0.207 ± 0.124
2.35AsnAsp: 2.35 ± 0.349
1.797AsnGlu: 1.797 ± 0.31
1.244AsnPhe: 1.244 ± 0.348
3.455AsnGly: 3.455 ± 0.62
0.898AsnHis: 0.898 ± 0.253
1.313AsnIle: 1.313 ± 0.417
1.106AsnLys: 1.106 ± 0.289
2.35AsnLeu: 2.35 ± 0.409
0.829AsnMet: 0.829 ± 0.248
0.76AsnAsn: 0.76 ± 0.216
2.419AsnPro: 2.419 ± 0.435
0.691AsnGln: 0.691 ± 0.338
1.451AsnArg: 1.451 ± 0.35
1.589AsnSer: 1.589 ± 0.337
1.659AsnThr: 1.659 ± 0.389
3.179AsnVal: 3.179 ± 0.361
0.622AsnTrp: 0.622 ± 0.198
1.037AsnTyr: 1.037 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
5.874ProAla: 5.874 ± 0.691
0.346ProCys: 0.346 ± 0.19
2.833ProAsp: 2.833 ± 0.422
4.492ProGlu: 4.492 ± 0.591
1.935ProPhe: 1.935 ± 0.339
5.252ProGly: 5.252 ± 0.82
1.382ProHis: 1.382 ± 0.274
2.281ProIle: 2.281 ± 0.384
2.972ProLys: 2.972 ± 0.934
3.939ProLeu: 3.939 ± 0.633
1.866ProMet: 1.866 ± 0.394
2.073ProAsn: 2.073 ± 0.439
2.281ProPro: 2.281 ± 0.454
3.11ProGln: 3.11 ± 1.178
2.972ProArg: 2.972 ± 0.513
2.557ProSer: 2.557 ± 0.358
4.354ProThr: 4.354 ± 0.659
3.939ProVal: 3.939 ± 0.549
0.76ProTrp: 0.76 ± 0.383
1.382ProTyr: 1.382 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
4.285GlnAla: 4.285 ± 0.585
0.138GlnCys: 0.138 ± 0.085
2.073GlnAsp: 2.073 ± 0.311
1.728GlnGlu: 1.728 ± 0.281
1.797GlnPhe: 1.797 ± 0.475
4.561GlnGly: 4.561 ± 1.934
0.484GlnHis: 0.484 ± 0.189
2.419GlnIle: 2.419 ± 0.348
1.451GlnLys: 1.451 ± 0.306
3.594GlnLeu: 3.594 ± 0.618
1.175GlnMet: 1.175 ± 0.569
0.622GlnAsn: 0.622 ± 0.212
2.281GlnPro: 2.281 ± 0.444
2.004GlnGln: 2.004 ± 0.663
3.11GlnArg: 3.11 ± 0.464
1.313GlnSer: 1.313 ± 0.304
2.419GlnThr: 2.419 ± 0.462
2.281GlnVal: 2.281 ± 0.395
0.76GlnTrp: 0.76 ± 0.211
0.622GlnTyr: 0.622 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
6.012ArgAla: 6.012 ± 0.682
0.76ArgCys: 0.76 ± 0.266
3.663ArgAsp: 3.663 ± 0.475
5.529ArgGlu: 5.529 ± 0.602
1.935ArgPhe: 1.935 ± 0.42
4.699ArgGly: 4.699 ± 0.555
1.451ArgHis: 1.451 ± 0.272
3.455ArgIle: 3.455 ± 0.426
3.594ArgLys: 3.594 ± 0.602
5.321ArgLeu: 5.321 ± 0.671
2.211ArgMet: 2.211 ± 0.44
1.797ArgAsn: 1.797 ± 0.346
2.142ArgPro: 2.142 ± 0.382
1.935ArgGln: 1.935 ± 0.333
5.874ArgArg: 5.874 ± 0.663
4.077ArgSer: 4.077 ± 0.56
3.525ArgThr: 3.525 ± 0.449
5.045ArgVal: 5.045 ± 0.555
1.52ArgTrp: 1.52 ± 0.308
2.764ArgTyr: 2.764 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
4.147SerAla: 4.147 ± 0.621
0.138SerCys: 0.138 ± 0.1
2.695SerAsp: 2.695 ± 0.448
3.594SerGlu: 3.594 ± 0.525
2.557SerPhe: 2.557 ± 0.426
5.045SerGly: 5.045 ± 0.852
0.691SerHis: 0.691 ± 0.215
2.695SerIle: 2.695 ± 0.332
2.903SerLys: 2.903 ± 0.512
4.423SerLeu: 4.423 ± 0.611
1.451SerMet: 1.451 ± 0.479
1.451SerAsn: 1.451 ± 0.297
2.903SerPro: 2.903 ± 0.347
2.419SerGln: 2.419 ± 0.504
3.663SerArg: 3.663 ± 0.56
2.142SerSer: 2.142 ± 0.341
2.281SerThr: 2.281 ± 0.429
3.317SerVal: 3.317 ± 0.486
1.106SerTrp: 1.106 ± 0.247
1.037SerTyr: 1.037 ± 0.247
0.0SerXaa: 0.0 ± 0.0
Thr
5.39ThrAla: 5.39 ± 0.65
0.276ThrCys: 0.276 ± 0.147
4.008ThrAsp: 4.008 ± 0.697
4.423ThrGlu: 4.423 ± 0.529
2.211ThrPhe: 2.211 ± 0.328
6.565ThrGly: 6.565 ± 1.424
0.691ThrHis: 0.691 ± 0.241
1.935ThrIle: 1.935 ± 0.439
2.488ThrLys: 2.488 ± 0.43
5.736ThrLeu: 5.736 ± 0.647
1.175ThrMet: 1.175 ± 0.282
1.659ThrAsn: 1.659 ± 0.379
4.008ThrPro: 4.008 ± 0.627
3.041ThrGln: 3.041 ± 0.493
3.801ThrArg: 3.801 ± 0.493
1.935ThrSer: 1.935 ± 0.368
3.732ThrThr: 3.732 ± 0.549
5.183ThrVal: 5.183 ± 0.717
1.037ThrTrp: 1.037 ± 0.253
2.211ThrTyr: 2.211 ± 0.377
0.0ThrXaa: 0.0 ± 0.0
Val
6.704ValAla: 6.704 ± 0.719
0.898ValCys: 0.898 ± 0.294
4.838ValAsp: 4.838 ± 0.617
4.768ValGlu: 4.768 ± 0.576
2.004ValPhe: 2.004 ± 0.489
4.907ValGly: 4.907 ± 0.537
1.589ValHis: 1.589 ± 0.333
3.179ValIle: 3.179 ± 0.407
3.525ValLys: 3.525 ± 0.453
5.252ValLeu: 5.252 ± 0.698
1.52ValMet: 1.52 ± 0.311
2.626ValAsn: 2.626 ± 0.431
3.525ValPro: 3.525 ± 0.505
2.211ValGln: 2.211 ± 0.369
4.907ValArg: 4.907 ± 0.632
4.976ValSer: 4.976 ± 0.562
4.008ValThr: 4.008 ± 0.572
5.39ValVal: 5.39 ± 0.542
1.313ValTrp: 1.313 ± 0.345
1.52ValTyr: 1.52 ± 0.289
0.0ValXaa: 0.0 ± 0.0
Trp
1.797TrpAla: 1.797 ± 0.354
0.276TrpCys: 0.276 ± 0.158
1.313TrpAsp: 1.313 ± 0.314
0.968TrpGlu: 0.968 ± 0.24
0.622TrpPhe: 0.622 ± 0.19
1.451TrpGly: 1.451 ± 0.354
0.346TrpHis: 0.346 ± 0.156
1.382TrpIle: 1.382 ± 0.337
0.138TrpLys: 0.138 ± 0.099
1.382TrpLeu: 1.382 ± 0.292
0.622TrpMet: 0.622 ± 0.183
1.106TrpAsn: 1.106 ± 0.332
1.037TrpPro: 1.037 ± 0.305
0.76TrpGln: 0.76 ± 0.24
1.106TrpArg: 1.106 ± 0.279
0.898TrpSer: 0.898 ± 0.251
1.52TrpThr: 1.52 ± 0.321
1.244TrpVal: 1.244 ± 0.244
0.553TrpTrp: 0.553 ± 0.252
0.553TrpTyr: 0.553 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.903TyrAla: 2.903 ± 0.53
0.346TyrCys: 0.346 ± 0.152
1.52TyrAsp: 1.52 ± 0.289
2.557TyrGlu: 2.557 ± 0.459
0.622TyrPhe: 0.622 ± 0.151
2.419TyrGly: 2.419 ± 0.457
0.484TyrHis: 0.484 ± 0.183
1.382TyrIle: 1.382 ± 0.284
0.76TyrLys: 0.76 ± 0.2
2.903TyrLeu: 2.903 ± 0.364
0.76TyrMet: 0.76 ± 0.228
1.175TyrAsn: 1.175 ± 0.24
1.382TyrPro: 1.382 ± 0.306
1.037TyrGln: 1.037 ± 0.239
2.211TyrArg: 2.211 ± 0.492
1.451TyrSer: 1.451 ± 0.328
1.659TyrThr: 1.659 ± 0.342
1.935TyrVal: 1.935 ± 0.349
0.553TyrTrp: 0.553 ± 0.211
1.175TyrTyr: 1.175 ± 0.332
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (14471 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski