Amino acid dipepetide frequency for Clostridioides difficile (strain 630) (Peptoclostridium difficile)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	2.923AlaAla: 2.923 ± 0.063	0.759AlaCys: 0.759 ± 0.026	2.569AlaAsp: 2.569 ± 0.049	2.978AlaGlu: 2.978 ± 0.06	2.362AlaPhe: 2.362 ± 0.058	3.664AlaGly: 3.664 ± 0.07	0.79AlaHis: 0.79 ± 0.028	5.722AlaIle: 5.722 ± 0.087	4.715AlaLys: 4.715 ± 0.064	5.44AlaLeu: 5.44 ± 0.082	1.67AlaMet: 1.67 ± 0.048	2.697AlaAsn: 2.697 ± 0.053	1.372AlaPro: 1.372 ± 0.038	1.453AlaGln: 1.453 ± 0.035	1.843AlaArg: 1.843 ± 0.053	3.38AlaSer: 3.38 ± 0.061	3.091AlaThr: 3.091 ± 0.084	3.848AlaVal: 3.848 ± 0.069	0.332AlaTrp: 0.332 ± 0.019	1.98AlaTyr: 1.98 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.642CysAla: 0.642 ± 0.027	0.238CysCys: 0.238 ± 0.015	0.759CysAsp: 0.759 ± 0.028	0.869CysGlu: 0.869 ± 0.031	0.479CysPhe: 0.479 ± 0.022	1.084CysGly: 1.084 ± 0.037	0.204CysHis: 0.204 ± 0.014	1.234CysIle: 1.234 ± 0.038	1.049CysLys: 1.049 ± 0.032	0.976CysLeu: 0.976 ± 0.029	0.377CysMet: 0.377 ± 0.016	0.739CysAsn: 0.739 ± 0.026	0.49CysPro: 0.49 ± 0.026	0.248CysGln: 0.248 ± 0.014	0.381CysArg: 0.381 ± 0.018	0.868CysSer: 0.868 ± 0.029	0.571CysThr: 0.571 ± 0.023	0.777CysVal: 0.777 ± 0.025	0.071CysTrp: 0.071 ± 0.008	0.417CysTyr: 0.417 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	2.686AspAla: 2.686 ± 0.05	0.667AspCys: 0.667 ± 0.024	2.929AspAsp: 2.929 ± 0.062	4.903AspGlu: 4.903 ± 0.072	2.752AspPhe: 2.752 ± 0.054	3.329AspGly: 3.329 ± 0.065	0.437AspHis: 0.437 ± 0.019	6.529AspIle: 6.529 ± 0.085	5.863AspLys: 5.863 ± 0.079	5.088AspLeu: 5.088 ± 0.067	1.688AspMet: 1.688 ± 0.038	3.323AspAsn: 3.323 ± 0.07	1.089AspPro: 1.089 ± 0.036	0.648AspGln: 0.648 ± 0.029	1.838AspArg: 1.838 ± 0.042	3.428AspSer: 3.428 ± 0.062	2.838AspThr: 2.838 ± 0.051	3.876AspVal: 3.876 ± 0.06	0.374AspTrp: 0.374 ± 0.017	2.698AspTyr: 2.698 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	3.835GluAla: 3.835 ± 0.07	0.769GluCys: 0.769 ± 0.03	4.443GluAsp: 4.443 ± 0.068	6.76GluGlu: 6.76 ± 0.11	3.014GluPhe: 3.014 ± 0.047	3.789GluGly: 3.789 ± 0.068	1.087GluHis: 1.087 ± 0.034	7.04GluIle: 7.04 ± 0.087	7.702GluLys: 7.702 ± 0.099	6.969GluLeu: 6.969 ± 0.1	1.961GluMet: 1.961 ± 0.045	5.55GluAsn: 5.55 ± 0.078	1.408GluPro: 1.408 ± 0.038	1.902GluGln: 1.902 ± 0.044	2.413GluArg: 2.413 ± 0.053	3.945GluSer: 3.945 ± 0.061	2.903GluThr: 2.903 ± 0.064	4.914GluVal: 4.914 ± 0.078	0.418GluTrp: 0.418 ± 0.021	3.16GluTyr: 3.16 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.341PheAla: 2.341 ± 0.047	0.541PheCys: 0.541 ± 0.02	2.579PheAsp: 2.579 ± 0.044	3.027PheGlu: 3.027 ± 0.054	2.007PhePhe: 2.007 ± 0.048	2.989PheGly: 2.989 ± 0.058	0.49PheHis: 0.49 ± 0.024	4.535PheIle: 4.535 ± 0.083	3.643PheLys: 3.643 ± 0.056	3.949PheLeu: 3.949 ± 0.069	1.242PheMet: 1.242 ± 0.036	2.741PheAsn: 2.741 ± 0.061	1.128PhePro: 1.128 ± 0.036	0.815PheGln: 0.815 ± 0.033	1.237PheArg: 1.237 ± 0.03	3.119PheSer: 3.119 ± 0.055	2.236PheThr: 2.236 ± 0.049	2.836PheVal: 2.836 ± 0.059	0.284PheTrp: 0.284 ± 0.016	1.725PheTyr: 1.725 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.067GlyAla: 4.067 ± 0.104	0.996GlyCys: 0.996 ± 0.031	3.074GlyAsp: 3.074 ± 0.069	4.02GlyGlu: 4.02 ± 0.067	3.16GlyPhe: 3.16 ± 0.055	4.217GlyGly: 4.217 ± 0.078	0.963GlyHis: 0.963 ± 0.028	6.797GlyIle: 6.797 ± 0.083	5.435GlyLys: 5.435 ± 0.078	5.42GlyLeu: 5.42 ± 0.073	1.848GlyMet: 1.848 ± 0.047	3.39GlyAsn: 3.39 ± 0.054	1.228GlyPro: 1.228 ± 0.066	1.491GlyGln: 1.491 ± 0.038	2.049GlyArg: 2.049 ± 0.044	3.729GlySer: 3.729 ± 0.063	3.265GlyThr: 3.265 ± 0.062	4.572GlyVal: 4.572 ± 0.074	0.472GlyTrp: 0.472 ± 0.027	3.051GlyTyr: 3.051 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	0.665HisAla: 0.665 ± 0.022	0.207HisCys: 0.207 ± 0.013	0.76HisAsp: 0.76 ± 0.027	0.869HisGlu: 0.869 ± 0.031	0.579HisPhe: 0.579 ± 0.025	0.934HisGly: 0.934 ± 0.029	0.265HisHis: 0.265 ± 0.017	1.4HisIle: 1.4 ± 0.035	1.068HisLys: 1.068 ± 0.032	1.204HisLeu: 1.204 ± 0.034	0.373HisMet: 0.373 ± 0.021	0.815HisAsn: 0.815 ± 0.023	0.563HisPro: 0.563 ± 0.022	0.355HisGln: 0.355 ± 0.018	0.453HisArg: 0.453 ± 0.022	0.862HisSer: 0.862 ± 0.029	0.75HisThr: 0.75 ± 0.025	0.791HisVal: 0.791 ± 0.024	0.097HisTrp: 0.097 ± 0.01	0.562HisTyr: 0.562 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.686IleAla: 5.686 ± 0.086	1.358IleCys: 1.358 ± 0.034	6.255IleAsp: 6.255 ± 0.091	7.279IleGlu: 7.279 ± 0.1	4.064IlePhe: 4.064 ± 0.083	6.369IleGly: 6.369 ± 0.088	1.289IleHis: 1.289 ± 0.032	9.061IleIle: 9.061 ± 0.122	8.669IleLys: 8.669 ± 0.096	9.183IleLeu: 9.183 ± 0.102	2.388IleMet: 2.388 ± 0.052	6.184IleAsn: 6.184 ± 0.087	3.398IlePro: 3.398 ± 0.061	2.304IleGln: 2.304 ± 0.048	2.992IleArg: 2.992 ± 0.053	7.313IleSer: 7.313 ± 0.096	4.747IleThr: 4.747 ± 0.074	6.631IleVal: 6.631 ± 0.093	0.48IleTrp: 0.48 ± 0.021	3.505IleTyr: 3.505 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.363LysAla: 4.363 ± 0.071	0.887LysCys: 0.887 ± 0.035	5.923LysAsp: 5.923 ± 0.084	8.784LysGlu: 8.784 ± 0.097	3.337LysPhe: 3.337 ± 0.054	4.928LysGly: 4.928 ± 0.072	1.205LysHis: 1.205 ± 0.033	8.26LysIle: 8.26 ± 0.109	8.566LysLys: 8.566 ± 0.09	7.718LysLeu: 7.718 ± 0.067	2.589LysMet: 2.589 ± 0.054	6.717LysAsn: 6.717 ± 0.089	2.042LysPro: 2.042 ± 0.047	2.465LysGln: 2.465 ± 0.049	2.88LysArg: 2.88 ± 0.049	5.665LysSer: 5.665 ± 0.073	4.091LysThr: 4.091 ± 0.061	5.861LysVal: 5.861 ± 0.067	0.52LysTrp: 0.52 ± 0.02	4.261LysTyr: 4.261 ± 0.069	0.0LysXaa: 0.0 ± 0.0
Leu	5.19LeuAla: 5.19 ± 0.078	1.106LeuCys: 1.106 ± 0.032	5.667LeuAsp: 5.667 ± 0.077	6.709LeuGlu: 6.709 ± 0.105	3.858LeuPhe: 3.858 ± 0.075	6.072LeuGly: 6.072 ± 0.08	1.102LeuHis: 1.102 ± 0.032	8.172LeuIle: 8.172 ± 0.102	8.444LeuLys: 8.444 ± 0.096	7.926LeuLeu: 7.926 ± 0.105	2.274LeuMet: 2.274 ± 0.051	5.967LeuAsn: 5.967 ± 0.072	2.747LeuPro: 2.747 ± 0.051	2.043LeuGln: 2.043 ± 0.046	2.951LeuArg: 2.951 ± 0.054	6.75LeuSer: 6.75 ± 0.083	4.433LeuThr: 4.433 ± 0.074	5.795LeuVal: 5.795 ± 0.078	0.527LeuTrp: 0.527 ± 0.021	3.218LeuTyr: 3.218 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	1.697MetAla: 1.697 ± 0.046	0.32MetCys: 0.32 ± 0.016	1.603MetAsp: 1.603 ± 0.038	1.88MetGlu: 1.88 ± 0.038	1.093MetPhe: 1.093 ± 0.034	1.818MetGly: 1.818 ± 0.041	0.348MetHis: 0.348 ± 0.02	2.458MetIle: 2.458 ± 0.052	2.506MetLys: 2.506 ± 0.05	2.419MetLeu: 2.419 ± 0.043	0.77MetMet: 0.77 ± 0.027	1.734MetAsn: 1.734 ± 0.04	0.889MetPro: 0.889 ± 0.032	0.681MetGln: 0.681 ± 0.022	0.879MetArg: 0.879 ± 0.03	1.923MetSer: 1.923 ± 0.04	1.298MetThr: 1.298 ± 0.032	1.692MetVal: 1.692 ± 0.04	0.152MetTrp: 0.152 ± 0.011	0.987MetTyr: 0.987 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.772AsnAla: 2.772 ± 0.048	0.768AsnCys: 0.768 ± 0.029	3.134AsnAsp: 3.134 ± 0.057	4.153AsnGlu: 4.153 ± 0.061	2.567AsnPhe: 2.567 ± 0.046	3.482AsnGly: 3.482 ± 0.067	0.857AsnHis: 0.857 ± 0.025	7.218AsnIle: 7.218 ± 0.082	6.172AsnLys: 6.172 ± 0.075	6.005AsnLeu: 6.005 ± 0.081	1.835AsnMet: 1.835 ± 0.044	4.286AsnAsn: 4.286 ± 0.087	2.219AsnPro: 2.219 ± 0.042	1.631AsnGln: 1.631 ± 0.04	2.047AsnArg: 2.047 ± 0.046	4.048AsnSer: 4.048 ± 0.075	3.077AsnThr: 3.077 ± 0.069	3.738AsnVal: 3.738 ± 0.062	0.418AsnTrp: 0.418 ± 0.018	2.585AsnTyr: 2.585 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	1.4ProAla: 1.4 ± 0.042	0.343ProCys: 0.343 ± 0.019	1.504ProAsp: 1.504 ± 0.046	2.024ProGlu: 2.024 ± 0.048	1.389ProPhe: 1.389 ± 0.035	1.561ProGly: 1.561 ± 0.045	0.474ProHis: 0.474 ± 0.019	2.852ProIle: 2.852 ± 0.047	2.285ProLys: 2.285 ± 0.046	2.333ProLeu: 2.333 ± 0.044	0.68ProMet: 0.68 ± 0.028	1.605ProAsn: 1.605 ± 0.04	0.628ProPro: 0.628 ± 0.032	0.773ProGln: 0.773 ± 0.028	0.725ProArg: 0.725 ± 0.025	1.705ProSer: 1.705 ± 0.043	1.695ProThr: 1.695 ± 0.067	2.048ProVal: 2.048 ± 0.047	0.227ProTrp: 0.227 ± 0.014	1.161ProTyr: 1.161 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	1.335GlnAla: 1.335 ± 0.036	0.26GlnCys: 0.26 ± 0.019	1.242GlnAsp: 1.242 ± 0.029	1.862GlnGlu: 1.862 ± 0.044	0.917GlnPhe: 0.917 ± 0.026	1.532GlnGly: 1.532 ± 0.034	0.332GlnHis: 0.332 ± 0.018	2.308GlnIle: 2.308 ± 0.045	2.059GlnLys: 2.059 ± 0.053	2.125GlnLeu: 2.125 ± 0.047	0.673GlnMet: 0.673 ± 0.021	1.577GlnAsn: 1.577 ± 0.038	0.593GlnPro: 0.593 ± 0.024	0.708GlnGln: 0.708 ± 0.027	0.976GlnArg: 0.976 ± 0.038	1.471GlnSer: 1.471 ± 0.039	1.186GlnThr: 1.186 ± 0.04	1.563GlnVal: 1.563 ± 0.038	0.167GlnTrp: 0.167 ± 0.013	0.995GlnTyr: 0.995 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	1.815ArgAla: 1.815 ± 0.052	0.382ArgCys: 0.382 ± 0.019	1.769ArgAsp: 1.769 ± 0.043	2.978ArgGlu: 2.978 ± 0.063	1.471ArgPhe: 1.471 ± 0.032	1.837ArgGly: 1.837 ± 0.041	0.469ArgHis: 0.469 ± 0.022	2.871ArgIle: 2.871 ± 0.059	3.001ArgLys: 3.001 ± 0.055	2.869ArgLeu: 2.869 ± 0.055	0.892ArgMet: 0.892 ± 0.025	1.965ArgAsn: 1.965 ± 0.05	0.896ArgPro: 0.896 ± 0.033	0.924ArgGln: 0.924 ± 0.031	1.273ArgArg: 1.273 ± 0.038	1.467ArgSer: 1.467 ± 0.035	1.492ArgThr: 1.492 ± 0.035	2.208ArgVal: 2.208 ± 0.044	0.21ArgTrp: 0.21 ± 0.012	1.416ArgTyr: 1.416 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	3.176SerAla: 3.176 ± 0.057	0.715SerCys: 0.715 ± 0.029	3.382SerAsp: 3.382 ± 0.061	4.053SerGlu: 4.053 ± 0.066	3.039SerPhe: 3.039 ± 0.062	4.225SerGly: 4.225 ± 0.073	0.932SerHis: 0.932 ± 0.03	7.086SerIle: 7.086 ± 0.092	6.348SerLys: 6.348 ± 0.091	6.055SerLeu: 6.055 ± 0.084	1.839SerMet: 1.839 ± 0.039	4.11SerAsn: 4.11 ± 0.07	1.592SerPro: 1.592 ± 0.039	1.721SerGln: 1.721 ± 0.036	2.165SerArg: 2.165 ± 0.046	4.521SerSer: 4.521 ± 0.075	3.323SerThr: 3.323 ± 0.057	4.144SerVal: 4.144 ± 0.059	0.412SerTrp: 0.412 ± 0.02	2.555SerTyr: 2.555 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	2.823ThrAla: 2.823 ± 0.057	0.561ThrCys: 0.561 ± 0.022	2.559ThrAsp: 2.559 ± 0.058	2.853ThrGlu: 2.853 ± 0.053	2.147ThrPhe: 2.147 ± 0.041	3.97ThrGly: 3.97 ± 0.162	0.834ThrHis: 0.834 ± 0.026	4.926ThrIle: 4.926 ± 0.075	3.788ThrLys: 3.788 ± 0.062	4.786ThrLeu: 4.786 ± 0.072	1.145ThrMet: 1.145 ± 0.031	2.766ThrAsn: 2.766 ± 0.06	1.787ThrPro: 1.787 ± 0.04	1.189ThrGln: 1.189 ± 0.034	1.534ThrArg: 1.534 ± 0.04	3.36ThrSer: 3.36 ± 0.06	2.629ThrThr: 2.629 ± 0.053	3.289ThrVal: 3.289 ± 0.061	0.32ThrTrp: 0.32 ± 0.014	1.989ThrTyr: 1.989 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	3.998ValAla: 3.998 ± 0.069	0.957ValCys: 0.957 ± 0.031	4.061ValAsp: 4.061 ± 0.065	4.741ValGlu: 4.741 ± 0.066	2.995ValPhe: 2.995 ± 0.06	4.538ValGly: 4.538 ± 0.079	0.828ValHis: 0.828 ± 0.025	5.942ValIle: 5.942 ± 0.077	5.43ValLys: 5.43 ± 0.081	6.296ValLeu: 6.296 ± 0.079	1.691ValMet: 1.691 ± 0.042	3.744ValAsn: 3.744 ± 0.052	1.94ValPro: 1.94 ± 0.047	1.38ValGln: 1.38 ± 0.03	1.986ValArg: 1.986 ± 0.048	4.656ValSer: 4.656 ± 0.071	3.23ValThr: 3.23 ± 0.056	4.848ValVal: 4.848 ± 0.083	0.386ValTrp: 0.386 ± 0.018	2.519ValTyr: 2.519 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.335TrpAla: 0.335 ± 0.017	0.096TrpCys: 0.096 ± 0.01	0.364TrpAsp: 0.364 ± 0.017	0.383TrpGlu: 0.383 ± 0.019	0.291TrpPhe: 0.291 ± 0.015	0.43TrpGly: 0.43 ± 0.02	0.114TrpHis: 0.114 ± 0.011	0.604TrpIle: 0.604 ± 0.024	0.499TrpLys: 0.499 ± 0.02	0.549TrpLeu: 0.549 ± 0.024	0.172TrpMet: 0.172 ± 0.013	0.393TrpAsn: 0.393 ± 0.02	0.141TrpPro: 0.141 ± 0.011	0.199TrpGln: 0.199 ± 0.017	0.224TrpArg: 0.224 ± 0.014	0.381TrpSer: 0.381 ± 0.017	0.296TrpThr: 0.296 ± 0.016	0.369TrpVal: 0.369 ± 0.018	0.071TrpTrp: 0.071 ± 0.008	0.289TrpTyr: 0.289 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.837TyrAla: 1.837 ± 0.037	0.54TyrCys: 0.54 ± 0.024	2.41TyrAsp: 2.41 ± 0.045	2.854TyrGlu: 2.854 ± 0.051	1.939TyrPhe: 1.939 ± 0.061	2.472TyrGly: 2.472 ± 0.046	0.554TyrHis: 0.554 ± 0.021	4.141TyrIle: 4.141 ± 0.067	3.819TyrLys: 3.819 ± 0.063	3.64TyrLeu: 3.64 ± 0.063	1.041TyrMet: 1.041 ± 0.033	2.712TyrAsn: 2.712 ± 0.055	1.262TyrPro: 1.262 ± 0.04	0.951TyrGln: 0.951 ± 0.032	1.362TyrArg: 1.362 ± 0.038	2.804TyrSer: 2.804 ± 0.053	2.103TyrThr: 2.103 ± 0.044	2.367TyrVal: 2.367 ± 0.052	0.263TyrTrp: 0.263 ± 0.013	1.781TyrTyr: 1.781 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3762 proteins (1161445 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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