Mycoplasmopsis columbinum SF7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis; Mycoplasmopsis columbina

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 614 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9UJX0|F9UJX0_9MOLU DNA-damage repair protein OS=Mycoplasmopsis columbinum SF7 OX=1037410 GN=MCSF7_00884 PE=3 SV=1
MM1 pKa = 7.48FKK3 pKa = 10.61QIDD6 pKa = 3.96LHH8 pKa = 8.28GLDD11 pKa = 4.69QIQALSKK18 pKa = 11.14VEE20 pKa = 4.16LAFLDD25 pKa = 4.02AQEE28 pKa = 4.67HH29 pKa = 6.18NISKK33 pKa = 10.24IEE35 pKa = 4.26IITGKK40 pKa = 10.32GSKK43 pKa = 8.45TLFTVVEE50 pKa = 4.7DD51 pKa = 3.87YY52 pKa = 11.56LMKK55 pKa = 10.23HH56 pKa = 6.7DD57 pKa = 4.14YY58 pKa = 10.88SYY60 pKa = 11.65AITNDD65 pKa = 2.87NGAFEE70 pKa = 5.09VYY72 pKa = 10.48LEE74 pKa = 3.75QDD76 pKa = 3.06YY77 pKa = 11.13WDD79 pKa = 5.3DD80 pKa = 3.95EE81 pKa = 4.67EE82 pKa = 4.83EE83 pKa = 5.07DD84 pKa = 3.93NYY86 pKa = 11.4CDD88 pKa = 3.89VLKK91 pKa = 10.34EE92 pKa = 4.09YY93 pKa = 10.05PFPEE97 pKa = 5.6DD98 pKa = 3.96EE99 pKa = 4.38NCCC102 pKa = 3.8

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9UJE5|F9UJE5_9MOLU Peptide/nickel transport system ATP-binding protein OS=Mycoplasmopsis columbinum SF7 OX=1037410 GN=MCSF7_03018 PE=4 SV=1
MM1 pKa = 7.67SLRR4 pKa = 11.84TYY6 pKa = 10.45QPNKK10 pKa = 8.2RR11 pKa = 11.84QHH13 pKa = 6.34AKK15 pKa = 7.64THH17 pKa = 5.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTANGRR29 pKa = 11.84KK30 pKa = 8.89VLAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.76GRR40 pKa = 11.84KK41 pKa = 8.93RR42 pKa = 11.84LTVSDD47 pKa = 3.65KK48 pKa = 11.1RR49 pKa = 3.83

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

614

0

614

228039

30

6244

371.4

42.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.849 ± 0.133

0.376 ± 0.027

5.423 ± 0.106

7.135 ± 0.098

5.234 ± 0.128

4.408 ± 0.115

1.373 ± 0.047

8.828 ± 0.16

9.996 ± 0.109

10.159 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.584 ± 0.068

8.388 ± 0.235

2.591 ± 0.046

3.517 ± 0.072

2.864 ± 0.062

6.282 ± 0.081

5.54 ± 0.174

5.53 ± 0.084

0.847 ± 0.035

4.076 ± 0.072
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski